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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences

C Sumantri

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Author-ID : 464956

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

Published : 28 Documents Claim Missing Document

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Articles

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Growth Hormone Gene Polymorphism and Its Association with Partial Cumulative Milk Yields of Holstein Friesian Dairy Cattle R Misrianti; A Anggraeni; E Andreas; C Sumantri
Media Peternakan Vol. 35 No. 3 (2012): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Growth hormone gene (GH gene) plays an important role in regulating body growth and in developing mammary gland, similar with its interaction to specific receptors. The GH gene has been considered as one of candidate gene associated with selection on lactation trait and milk production. This study was aimed to determine genetic polymorphism of the GH-AluI gene and to associate its genotype variants on various 15-d partial cumulative milk yields in Holstein Friesian (HF) dairy cows. A number of 370 blood samples were collected from six HF populations, respectively from small dairy farmer under the supervision of the North Bandung Milk Cooperation (NBMC) in Cilumber (98) and Pasir Kemis village (96), Dairy Cattle Breeding and Improvement Station (Cikole DCBIS) Cikole (88), Lembang Artificial Insemination Center (Lembang AIC) (17), Singosari Artificial Insemination Center (Singosari AIC (32), and Cipelang Livestock Embryo Center (Cipelang LEC) (40). A polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP) method was used to identify variant genotypes of the GH gene using AluI restriction enzyme. Genotyping results produced only two genotypes, i.e. LL and LV genotypes, without VV genotype. Frequency of the former was dominant, whilst that was low for the latter (89% vs. 11%); leading to the frequency of L allele was very high (94%) compared to that of V allele (6%). No significant association between variant genotypes (LL and LV) and various 15-d partial cumulative milk yields.

Histomorphology and Physical Characteristics of Buffalo Meat at Different Sex and Age H Nuraini; . Mahmudah; A Winarto; C Sumantri
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The aim of this experiment was to evaluate histology and physical variables of buffalo meat at different sex and ages. Thirty head of buffaloes were used in this experiment. Muscle fiber diameter, fasciculus diameter, thickness of connective tissue, pH, water holding capacity, Warner-Bratzler shear force and cooking loss were measured in this experiment. Analysis of variance of randomized factorial design was used to analyse the data. The differences were analysed by Tukey test. The result showed that muscle fiber diameter was influenced by ages. The difference muscle fiber diameter among ages indicated that 8-12 mo and 1.5 yr old have smaller diameter compared to three year old (P<0.05), but there was no differences between two, three and four years old. The fasciculus diameter, thickness of connective tissue, tenderness, pH value, water holding capacity, and cooking lost indicated no significant found in all stage of age and sex of samples. Meat qualities of buffaloes were not affected by age (2-4 yr) and sex.

Identification of Single Nucleotide Polymorphism on Growth Hormone Gene in Aceh Cattle E M Sari; R R Noor; C Sumantri; M Yunus; Han J L; . Muladno
Media Peternakan Vol. 36 No. 1 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This research was aimed to identify the changes of nucleotide (Single Nucleotide Polymorphism) growth hormone gene in the population of Aceh cattle. There were 44 samples of DNA sequenced, and a few samples from Gen Bank (M57764). Based on the analysis using MEGA program, it was identified one new mutation on exon five on 2230 bp in which C nucleotide turned into T nucleotide, and this was called Silent Mutation (Leusine-Leusine/ CTC-CTT). The frequency of Single Nucleotide Polymorphism (SNP) genotype on 2230 bp (C/T) was CC (0.36), TT (0.14) and CT (0.50). The genotype TT was not possessed by Aceh cattle from Saree, but possessed by those from Banda Aceh and Indrapuri. Chi-square test showed not significant differences in allele frequencies for three population. The frequency of genotype SNP on 2291 bp (A/C) was AC (0.11) and CC (0.89). The frequency of allele C was higher than allele A and T.

Microanatomical Structure and Physical Characteristics of Thin Tail Hogget with Calpastatin (CAST-1) Genotype Differences B W Putra; C Sumantri; . Nurhidayat
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Thin tail sheep has good adaptation in tropics condition, but they have low meat quality. Quality of thin tail hogget can be improved by selection. Calpastatin (CAST) gene is an indigenous inhibitor of calpain that involved in regulation of protein turn over and growth. The objective of this research was to determine the effect of calpastatin-genotype on microanatomical structure and physical characteristics of thin tail hodget. Nine thin tail sheep from Jonggol were used for this research.PCR-RFLP method was carried out to identify genetic variation of calpastatin gene, based on the identificationof CAST variation genotype. It was found that MM and MN genotypes forcalpastatin gene with TT as asingle Calpain genotype variation. The sheep wasclustered based on the variation of calpastatin gene, 5 sheep hadMM genotype and 4 sheep hadMN genotype. Physical and microanatomical characteristics were analyzed from their meats. Sheep with MN genotype showed tougher meat, it was characterized with a greater of muscle fiber surface area, the number of muscle per muscle bundle and muscle bundle area and harder meat tenderness than in MM genotypes. Hypertrophy and hyperplasia of MN were greater than MM.

Avian Sex Determination Based on Chromo Helicase DNA-binding (CHD) Genes Using Polymerase Chain Reaction (PCR) I Khaerunnisa; E Sari; M Ulfah; . Jakaria; C Sumantri
Media Peternakan Vol. 36 No. 2 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Several bird species are sexually monomorphic. In this case, molecular approach is an efficient method for their sex determination. The sexes of monomorphic birds can be determined by PCR amplification of the CHD genes. CHD genes are preserved within avian Z and W sex chromosomes. The objective of this research was to determine sex of 21 individuals quails, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos based on CHD genes using two pairs of primers: 2550F-2718R and P2-P8. Samples from Kampong chickens and ducks were used as controls. PCR products were screened by agarose gel electrophoresis with ethidium bromide. Results showed that 2550F and 2718R primers could be used to identify sex of Kampong chickens, quails, ducks, pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos. Individuals showed double (ZW) and single (ZZ) bands were identified as females and males, respectively in Kampong chickens, quails, hill myna, salmon-crested cockatoos and yellow-crested cockatoos. Males and females in ducks and pigeons showed single band in different length of base pairs. P2 and P8 primers could be used to identify the sex of pigeons, hill myna, salmon-crested cockatoos, and yellow-crested cockatoos. All of those samples showed bouble (ZW) bands for females and single (ZZ) band for males.

Genetic Variation of mtDNA Cytochrome Oxidase Subunit I (COI) in Local Swamp Buffaloes in Indonesia F Saputra; . Jakaria; C Sumantri
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

The objective of this research was to identify genetic variation of mitochondria DNA especially in cytochrome oxidase subunit I (COI) among population of Indonesian buffaloes. Samples of swamp buffaloes were collected from Aceh (n= 3), North Sumatra (n= 3), Riau (n= 3), Banten (n= 3), Central Java (n= 3), West Nusa Tenggara (n= 3) and South Sulawesi (n= 3), and riverine buffalo from North Sumatra (n= 1) out of group for comparison. Sequence of COI was analyzed using MEGA 5.10 software with neighbor-joining method kimura 2-parameter model to reconstruct phylogeny tree. The result showed that three haplotypes for swamp buffalo and one haplotype for riverine buffalo in Indonesia resulted from 41 polymorphic sites. This finding showed that the COI gene could be considered as a marker to distinguish among swamp buffaloes in Indonesia.

Species Authentication of Dog, Cat, and Tiger Using Cytochrome β Gene . Irine; H Nuraini; C Sumantri
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Adulteration of animal food products for economic reason has happened during the last decades. Species identification method development was needed to prevent falsification information. The objective of this research was to study species authentication (dog, cat, and tiger) to ensure animal origin in products using cyt β gene specific marker. DNA extraction and fragment amplification were conducted using phenol-chloroform and multiplex PCR (Polymerase Chain Reaction) method, respectively. This research showed that fragment length of amplification for species tested (dog, cat, and tiger) were 523, 331, 319 bp, respectively. Species specificity was also indicated by high reverse primers homology percentage. Multiplex PCR technique succeed to amplify DNA fragment from species tested, but has a limitation to amplify total DNA composite of mix DNA.

GHRH|HaeIII Gene Polymorphism in Dairy and Beef Cattle at National Livestock Breeding Centers A O Rini; C Sumantri; A Anggraini
Media Peternakan Vol. 36 No. 3 (2013): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

This study was aimed to identify polymorphism of growth hormone releasing hormone (GHRH) gene in 89 heads of Holstein-Friesian (HF) dairy cattle from Lembang Artificial Insemination Center/LAIC (17 bulls), Singosari Artificial Insemination Center/SAIC (32 bulls), and Cipelang Livestock Embryo Center/CLEC (40 cows); as well as in 4 breeds of female beef cattle from CLEC for comparison, providing Simmental (13 cows), Limousin (14 cows), Brahman (5 cows), and Angus (5 cows). This study used PCR-RFLP method by using HaeIII restriction enzyme. The enzyme cut the GHRH gene at nucleotides of GG|CC at the base positions of 118, 312, and 406 and produced 4 fragments of 118, 194, 94, and 45 bp respectively. Genotyping the GHRH gene produced two types of allele, namely A (312, 94, and 45 bp) and B (194, 118, 94, and 45 bp). These two alleles resulted in three types of genotype, namely AA (312, 94, and 45 bp), AB (312, 194, 118, 94, and 45 bp), and BB (194, 118, 94, and 45 bp). Frequency of the B allele was dominant to the A allele. Chi-Square analysis showed that all of HF dairy and beef cattle observed were in Hardy-Weinberg equilibrium (X2 < X2(0.05)). The highest heterozygosity value was 0.471 for HF bulls in LAIC, while the lowest one was for HF bulls in SAIC. Heterozygosity values in Simmental and Limousin cattles were higher than that of in HF cattle. The GHRH gene in HF and beef cattle was polymorphic, the exception was for Brahman with the only B allele. This result will improve the understanding of the polymorphism of GHRH gene in dairy and beef cattle.

Analisis Hubungan Keragaman Gen SCD1 (Stearoyl CoA Desaturase) dengan Komposisi Asam lemak Daging Sapi Lokal di Ciamis Nena Hilmia; R.R Noor; C Sumantri; R Priyanto; Gurnadi E
Jurnal Ilmu Ternak Vol 16, No 2 (2016)
Publisher : Fakultas Peternakan, Universitas Padjadjaran

SCD1 merupakan kandidat gen yang berperan dalam mengkonversi asam lemak jenuhmenjadi tidak jenuh, dan rasio keduanya menentukan kualitas daging. Single NucleotidePolymorphism (SNP) V293A pada gen SCD1 yang mengubah asam amino Valina menjadiAlaninaberpengaruh terhadap komposisi asam lemak daging. Penelitian ini bertujuan mengidentifikasi keragaman gen SCD1 berdasarkan SNP V293A, dan hubungannya dengan kandungan asam lemak pada sapi lokal di Ciamis. Penelitian ini menggunakan 14 sampel daging dan DNA dengan genotipe yang berbeda. Metode PCR-RFLPdengan enzim Aci1digunakan untuk mendeteksi SNP V293A pada exon 5. Hubungan keragaman gen SCD1 dengan komposisi asam lemak daging dianalisis dengan uji T. Hasil penelitian ini menunjukkan gen SCD1 pada sapi lokal Ciamis polimorfik, terdapat dua alel, yaitu alel T dan C dengan tiga genotipe, yaitu TT, CT dan CC. SNP V293A pada gen SCD1 tidak berpengaruh terhadap komposisi asam lemak daging sapi lokal di Ciamis Jawa Barat.Kata kunci : asam lemak daging, PCR-RFLP, Sapi lokal Ciamis, SCD1.

Identification of Single Nucleotide Polymorphism (SNP) in Mono Amine Oxidase A (MAO-A) Gene as a genetic marker for aggressiveness in sheep Eko Handiwirawan; Ronny R Noor; C Sumantri; Subandriyo .; I Inounu
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 4 (2012): DECEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v17i4.692

In the population, there are aggressive sheep in a small number which requires special management those specific animal house and routine management. The purpose of this study was to identify the variation of DNA marker SNP (single nucleotide polymorphism) as a genetic marker for the aggressive trait in several of sheep breed. The identification of point mutations in exon 8 of MAO-A gene associated with aggressive behavior in sheep may be further useful to become of DNA markers for the aggressive trait in sheep. Five of sheep breed were used, i.e.: Barbados Black belly Cross sheep (BC), Composite Garut (KG), Local Garut (LG), Composite Sumatra (KS) and St. Cross Croix (SC). Duration of ten behavior traits, blood serotonin concentrations and DNA sequence of exon 8 of MAO-A gene from the sheep aggressive and nonaggressive were observed. PROC GLM of SAS Ver. 9.0 program was used to analyze variable behavior and blood serotonin concentrations. DNA polymorphism in exon 8 of MAO-A gene was analyzed using the MEGA software Ver. 4.0. The results show that the percentage of the aggressive rams of each breed was less than 10 percent; except for the KS sheep is higher (23%). Based on the duration of behavior, aggressive sheep group was not significantly different with non aggressive sheep group, except duration of care giving and drinking behavior. It is known that concentration of blood serotonin in aggressive and non aggressive rams was not significantly different. The aggressive trait in sheep has a mechanism or a different cause like that occurs in mice and humans. In this study, aggressive behavior in sheep was not associated with a mutation in exon 8 of MAO-A gene. Key Words: Sheep, Aggressive, Exon 8 Of MAO-A Gene, Single Nucleotide Polymorphism

Co-Authors . Irine . Mahmudah . Maskur A Anggraeni A Anggraini A Einstiana A Muzani A O Rini A Primasari A Tapyadi A Winarto ACHMAD FARAJALLAH B Brahmantiyo B Tiesnamurti B W Putra Bambang Ngadji Utomo Bambang Ngaji Utomo C Arman D Perwitasari E Andreas E Gurnadi E M Sari E Sari E.D Gunardi Eko Handiwirawan F Saputra Gurnadi E H Nuraini Han J L I Inounu I Khaerunnisa I Supriatna J F Salamena J.F Salamena Jakaria Jakaria M I.A Dagong M Yamin M Yunus Maria Ulfah Muhammad Ihsan Andi Dagong Nena Hilmia Niken Ulupi Nurhidayat - R Diyono R Herman R Misrianti R Priyanto R R Noor R.R Noor R.R. Noor Ronny R Noor S S Mansjoer Subandriyo . Sutikno Sutikno T Kertanugraha

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