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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Ternak Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences

C Sumantri

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Author-ID : 464956

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary

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Morphometric performances of Katingan cattle in Central Kalimantan Bambang Ngadji Utomo; R.R Noor; C Sumantri; I Supriatna; E.D Gunardi
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 3 (2010): SEPTEMBER 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The information of Katingan cattle as an indigenous cattle in Central Kalimantan is very limited, especially phenotypic characterization. A number of 72 heads, consist of 57 heads female and 15 heads male of Katingan cattle in adults category were used in this study. The samples were obtained from three sub districts in district of Katingan, those were Tewah Sanggalang Garing (Pendahara Village), Pulau Malan (Buntut Bali Village) and Katingan Tengah (Tumbang Lahang Village). The location were used as the variable of groups (subpopulation). This study was conducted to describe morphometric performance, which was analysed by discriminant and canonical to estimate the phylogenic relationship and to determine the discriminant variable among the group (subpopulation). The SAS package program version 6.0 and MEGA 2 program was used to analyze data. The observed parameters were body length, wither height, chest width, chest depth, chest circumference, hip heigth, hip width, head height, head length and head width. The chest depth and hip height of female cattle from group of Buntut Bali were lower (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The parameter of: chest width, hip width and body length of Buntut Bali groups were higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups. The average body weight of male Katingan cattle from Buntut Bali was higher (P < 0.05) than that of Pendahara and Tumbang Lahang groups but there were not different (P > 0.05) on bodyweight of female Katingan cattle among location. The highest similarity value was Katingan cattle from group of Tumbang Lahang (92,86%) followed by group of Pendahara (40.91%) and group of Buntut Bali (38.89%), repectivelly. Katingan cattle from group of Pendahara and Tumbang Lahang had closest genetic distance (1.406), while the longest genetic distance (2.046) was between group of Buntut Bali and group of Tumbang Lahang. Genetic distance between group of Buntut Bali and Pendahara was 1.929. Katingan cattle from Pendahara and Tumbang Lahang within one cluster. Body length in canonical structure 2 can be used as discriminant variable. Key Words: Katingan Cattle, Morphometric, Genetic Distance, Discriminant Analysis, Canonical

The qualitative variation of Katingan cattle Bambang Ngaji Utomo; R.R. Noor; C Sumantri; I Supriatna; E.D Gunardi; B Tiesnamurti
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The characterization is main activities in order to identify important trait of Katingan cattle. Information of its phenotypic characteristic is not available yet. The aim of the study is to describe the qualitative characteristic of Katingan cattle. Three location of the study were sub-district of Tewah Sanggalang Garing (Pendahara Village), sub-district of Pulau Malan (Buntut Bali Village), and sub-district of Katingan Tengah (Tumbang Lahang Village). The samples of Katingan cattle were taken as many as possible based on the field conditions to observe pattern of colour, growth of horn and gibbosity appear among two horns. General characteristic of Katingan cattle was various in colours of coat, having horns, humped and dewlop. The main characteristics were expressed at female of Katingan cattle. There were six variations of horn shape at female, however the growth of arching forwards was dominant (78.4%). Horn shape of male was generally grown upside (98.3%). Gibbosity was founded among horn only at female cattle (92.6%). There were 9 combinations of coat colour of female Katingan Cattle, those were brown reddish (27%), brown whitish (14.1%), brown like colour of Bali cattle (13.8%), black (12.5%), brown dull (9.6%), brown sorrel (9.3%), blackish (7.1%), white brownish (5.5%) and white grayish (4.5%). Male of Katingan cattle had eight colour combinations, those were brown whitish (14.8%), brown whitish and reddish (14.8%), brown reddish (13.1%), blackish (12.3%), brown whiteish with black withers (10.7%), brown sorrel (9.8%) and brown sorrel with black withers (7.8%). Based on qualitative analysis, the Katingan cattle had variations in coat of colour, growth of horn and the gibbosity. The variation of coat colour had potentially selection related to the value of cultural. Key Words: Katingan Cattle, Qualitative, Coat Colour, Horn, Gibbosity

Behaviour and production traits correlation of five breeds of sheep Eko Handiwirawan; R.R Noor; C Sumantri; Subandriyo .
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 3 (2012): SEPTEMBER 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Information about correlations among behavioral traits and production traits may facilitate using easily measured traits to be used as alternative criteria for indirect selection to improve other traits in sheep. The purpose of this study was to estimate correlations among behavioral and production traits of five breeds of sheep. A total of 128 head of weaned lambs and 168 ewes with nursing lambs of five breeds were used in this study, i.e. Barbados Black Belly Cross (BC), Composite Garut (KG), Local Garut (LG), Composite Sumatra (KS) and St. Croix Cross (SC). Temperament was assessed in a test arena, for subsequent association with growth rate and ewe productivity. Analysis of variance of production and behavioral traits among breeds was carried out using PROC GLM of SAS software Ver. 9.0. PROCCORR was used to estimate correlations among production and behavioral variables. Weaned lambs that were more docile towards the observer had higher daily gains. Ewes that bleated more frequently when separated from their lamb had higher lamb weaning weights and lamb survival than ewes bleating fewer times under the same conditions. In BC ewes, a positive correlation was observed between frequency of bleats and lamb survival. Key Words: Sheep, Behavior, Growth rate, Ewe productivity

Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 – exon 6) genotypes variation Muhammad Ihsan Andi Dagong; R Herman; C Sumantri; R.R Noor; M Yamin
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

Polymorphism of Calpastatin gene and its effect on body weight of local sheeps C Sumantri; R Diyono; A Farajallah; I Inounu
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The objectives of this research were to identify polymorphism of calpastatin gene and to investigate any association of calpastatin genotype on body weight of local sheeps. A total number of DNA samples were collected from 288 heads of local sheeps from 8 populations. Two local sheep samples were medium tail sheeps (MTSs) of a Garut fighting type from Ciomas/Bogor (29) and a Garut meat type from Margawati (29). The remaining six local sheep population were one thin tail sheep (TTS) from Jonggol (36); and five fat tail (FTSs) from Indramayu (43), Madura (43), Sumbawa (26), Rote (36) and Donggala (46) respectively. Genomic DNAs of those blood of local sheeps were extracted by a standard phenol-chloroform protocol and amplified using a polymerase chain reaction (PCR) technique. PCR reaction was carried out in a thermocycler (Takara PCR of Thermal Cycler MP4) and PCR products were digested with Msp 1 enzyme restriction using a Restriction Fragment Length Polymorphism (RFLP) technique. The PCR-RFLP products were separated at 8% polyacrylamide gel electrophoresis (PAGE). A silver-staining method then was applied to detect fragments. Genetic variations between local sheep populations were calculated based on frequencies of genotypes and alelles. The association between genotype of calpastatin gene and body weight of local sheeps were calculated by General Linear Model method by SAS version 6.12. A length of 622 base pairs (bp) of the calpastatin gene of the Indonesian local sheeps was successfully amplified by the PCR technique. An MspI restriction enzyme cut the PCR product into two different length fragments, those were 336 bp and 286 bp designated as M allele of the CAST-Msp1; whilst that unsuccessfully cut PCR product resulted one fragment 622 bp designated as N allele of the CAST-Msp1. Locus of the CAST-Msp1 gene in most local sheeps studied was polymorphic, the exception was in the FTS from Rote of which monomorphic. The highest frequency of the M allele was in the fighting Garut sheep from Ciomas (0.29), whilst the lowest was in the FTS from Rote (0.00). However, frequencies of the M allele of FTSs from Sumbawa and Madura were similar (0.04). Further, frequencies of the M allele of local sheeps from Margawati, Jonggol, Indramayu, and Donggala were 0.24, 0.16, 0.13 and 0.12 respectively. The highest frequency of MN genotype was observed in the Garut fighting sheep from Ciomas (0.58), but the lowest was in the FTS from Rote (0.00). The heterosigosity was observed differently among populations. The highest heterosigosity was also identified in the Garut fighting sheep in Ciomas (0.43), whilst FTSs both in Sumbawa and Madura were for the lowest (0.08). Results of this study showed that there was a definit association betwen calpastatin genotype and body weight of male sheeps from which the MN genotype significantly related to a higher body weight compared to that of the NN genotype. Key Words : Local Sheeps, Calpastatin Gene, Polymorphism, Live Weight

Performances and phylogenic relationships among local sheep in Indonesia by morphological analysis C Sumantri; A Einstiana; J.F Salamena; I Inounu
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 1 (2007): MARCH 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The morphological discriminant and canonical analysis were carried out to estimate the phylogenic relationship and determine the discriminant variables between Indonesian local sheep of thin tail sheep from Jonggol (Bogor) and Garut and fat tail sheep from Indramayu (originated from East Java), Donggala, Madura, Kisar, Rote and Sumbawa. The number of sheep used was totally 818 heads collected from 8 populations, were Jonggol (185), Garut (74), Indramayu (100), Donggala (60), Madura (86) Kisar (231), Rote (52), and Sumbawa (30). Discriminant analysis used for body weight and body measurements were body length, wither height, chest width, chest depth, chest circumference, skull length, skull width, skull height, tail lenght, tail width, ear length and ear width. SAS package program was used to analyze the data. The results from analysis variant showed that the body weight and body measurenment of Garut sheep almost the same with sheep from Indramayu and significantly higher (P<0,05) compared to another breeds. Whereas, Kisar and Rote have body weight and body measurenment significantly lower (P<0,05) compared to another breeds. The results from Mahalanobis distance (phenogram tree) and canonical analyses showed that breeds were divided into five groups, the first group is Garut, the second is thin-tail sheep which existed in Jonggol, the third was fat-tailed sheep which existed in Kisar, Rote, and Sumbawa, the fourth is fat-tail sheep which existed in Donggala, and the fiveth was fat-tailed sheep which existed in Indramayu and Madura. The groups of sheep between Kisar and Rote had the smallest genetic distance value equal to 1,623 and groups between Donggala and Garut had the biggest genetic distance value equal to 7,994. The highest similarity between individual inside the group was obtained from Sumbawa (100%) and the lowest similarity was from Rote (72,41%). The results from canonical analyses showed that tail lenght, ear width, ear lenght, skull lenght, body lenght, and tail width were the most discriminant variables to determine the diferences between breeds. Key Words: Sheep, Genetic Distance, Analysis Discriminant and Cannonica

Variation on DNA microsatellite of Holstein-Friesian dairy cows in Baturaden Dairy Cattle Breeding Center C Sumantri; A Anggraeni; A Farajallah; D Perwitasari
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Eight microsatellite loci of which the locations were three on chromosome 6 (BTA6) namely CSN 3, BM 143 and BM 415; two on chromosome 9 (BTA9) namely ETH 225 and BM 4208; and three on chromosome 10 (BTA10) namely BP 31, BM 1237 and BM 888 were used to investigate genotypic variation of Holstein-Friesian (HF) dairy cows in Baturraden Dairy Cattle Breeding Centre. Research activities were carried out through some steps involving blood collection, DNA isolation, amplification on DNA fragments by polymerase chain reaction (PCR) and separation by electrophoresis with silver staining. Frequency and heterozygosity of genes under consideration were calculated. The results showed that the eight microsatellite loci exhibited a total number of 33 alleles. Locations of those alleles were five in BM 143 and BM 4208 loci; four in BM 415, CSN 3, ETH 225, BM 1237, BM 888 loci; and three in BP 31 locus. A number of 14 out of 33 alleles might be specific alleles for HF dairy cattle in Baturraden Dairy Cattle Breeding Centre. The lowest heterozygosity per locus (ĥ) was 0.6151 for BM 415 whilst the highest one was 0.7301 for BM 888. Additionally, the average heterozygosity for all loci (Ĥ) detected in this study was 0.6768. The genotype BB on locus BM 143 and AC on locus CSN-3 significantly affected (P<0.05) the estimated breeding value of milk yield of HF cows in this location. Key Words: Holstein-Friesian Cows, DNA Microsatellite, Allele, EBV of Milk Yield

The Role of Triglyceride Lipase, Fatty Acid Synthase and Fatty Acid Binding Protein Family Genes on Fat Metabolism of Broiler Chickens Niken Ulupi; C Sumantri
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 25, No 1 (2015): MARCH 2015
Publisher : Indonesian Center for Animal Research and Development

Broiler chicken has high fat content, especially abdominal and subcutan fat which reduced carcass quality and efficiency of feed energy. Genetic approach could be potentially applied to reduce high abdominal and intramuscular fat in broiler chicken through the selection program at gene level related to fat metabolism. This paper describes the metabolism and biosynthesis of body fat and the role of its controlled genes. Fat synthesis is controlled by feed quality and metabolism and biosynthesis process occurred in liver. These processes are controlled by many family genes, but certain genes have dominant role in the process; those are triglyceride lipase genes, fatty acid synthase genes and fatty acid binding protein genes. Expression of fatty acid synthase genes has positive correlation with fat content in liver and intramuscular. Expression of fatty acid binding protein genes was related to the increased abdominal fat deposit. These genes are polymorphic, so that they can be used as a genetic marker in selection to optimize feed efficiency, to minimize abdominal fat and to increase economic value of broiler chicken. Key words: Fat, triglyceride lipase genes, fatty acid synthase genes, fatty acid binding protein genes

Co-Authors . Irine . Mahmudah . Maskur A Anggraeni A Anggraini A Einstiana A Muzani A O Rini A Primasari A Tapyadi A Winarto ACHMAD FARAJALLAH B Brahmantiyo B Tiesnamurti B W Putra Bambang Ngadji Utomo Bambang Ngaji Utomo C Arman D Perwitasari E Andreas E Gurnadi E M Sari E Sari E.D Gunardi Eko Handiwirawan F Saputra Gurnadi E H Nuraini Han J L I Inounu I Khaerunnisa I Supriatna J F Salamena J.F Salamena Jakaria Jakaria M I.A Dagong M Yamin M Yunus Maria Ulfah Muhammad Ihsan Andi Dagong Nena Hilmia Niken Ulupi Nurhidayat - R Diyono R Herman R Misrianti R Priyanto R R Noor R.R Noor R.R. Noor Ronny R Noor S S Mansjoer Subandriyo . Sutikno Sutikno T Kertanugraha

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