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Journal : agriTECH

Pengaruh Konsumsi Tempe Kedelai Hitam terhadap Aktivitas Makrofag dan Kadar Interleukin 1(IL-1) pada Tikus Secara in vivo Nurrahman Nurrahman; Nurhidajah Nurhidajah
agriTECH Vol 35, No 3 (2015)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (600.727 KB) | DOI: 10.22146/agritech.9340

Abstract

Black soybean tempe diet could increase the stimulation index of proliferation T cells, lymphocytes durability of hydrogen peroxide and the enzyme activity of SOD.The purpose of this study was to determine the effect of black soybean tempe consumption on on the activity of macrophages and the levels of IL-1 in rat in vivo. A total of 30 rats were grouped into 5 (five), each group of rats were 6. For 30 days each group was maintained by administering a standard diet and diet plus black soybean tempe flour (25, 50, 75 and 100% instead of casein). After the peritoneal fluid was taken to be used for analysis of macrophages activity and the levels of IL-1. The results showed highe rnumber of black soybean tempe in the feed, the higher the index of phagocytosis and the levels of IL-1. Consumption of black soybean tempe effect on macrophages activity and the levels of IL-1 (p < 0.05). Increased macrophage activity was positively correlated to the amount of IL-1, by 0.9 coefficient of correlation.ABSTRAKDiit tempe kedelai hitam dapat meningkatkan indeks stimulasi prolifersi sel T, meningkat daya tahan limfosit dari hidrogen peroksida dan aktivitas enzim SOD. Tujuan penelitian ini untuk mengetahui pengaruh konsumsi tempe kedelai hitam terhadap aktivitas makrofag dan kadar IL-1pada tikus secara in vivo. Sebanyak 30 ekor tikus dikelompokan menjadi 5 (lima), masing-masing kelompok sebanyak 6 ekor tikus. Selama 30 hari masing-masing kelompok dipelihara dengan pemberian diit standar dan diit ditambah tepung tempe kedelai hitam (25, 50, 75 dan 100% sebagai ganti kasein). Setelah itu diambil cairan peritoneal yang akan digunakan untuk analisis aktivitas makrofag dan kadar IL1. Hasil penelitian menujukkan semakin tinggi jumlah tempe kedelai hitam di dalam pakan, semakin tinggi indeks fagositosis dan kadar L-1. Konsumsi tempe kedelai hitam berpengaruh terhadap aktivitas makrofag dan kadar IL-1(p<0,05). Peningkatan aktivitas makrofag berkorelasi positif terhadap jumlah IL-1, dengan koefisien korelasi 0,9.
Efek Hipoglikemik Kecambah Beras Merah pada Tikus yang Diinduksi STZ-NA dengan Parameter Kadar Insulin, Indeks HOMA-IR dan HOMA β Nurhidajah Nurhidajah; Nurrahman Nurrahman
agriTECH Vol 36, No 4 (2016)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (626.019 KB) | DOI: 10.22146/agritech.16767

Abstract

The process of germination of grains such as rice, could increase some nutritional values of  amino acids and dietary fiber. Red rice and its sprouts are believed to be able to decrease blood glucose in patients with diabetes mellitus (DM). The aim of this study was to evaluate the hypoglycemic effect of red rice sprouts in STZ-NA induced diabetic rats on blood glucose level, insulin level, and HOMA-IR and HOMA-β index. This experimental study was conducted based on randomized post test only control group design using 24 male Wistar rats aged 2.5 months. Rats were divided into 4 groups, one group without induction of STZ-NA fed with a standard diet (control) and three groups of STZ- NA induced with a standard diet, red rice and red rice germ. Experiments were conducted for 6 weeks. The results showed that sprouted red rice lowered blood glucose levels by 61.88 % and the value of HOMA-IR (insulin resistance parameters) by 56.82%. Insulin level increased by 16.35 % and HOMA-β by 763.6 %. This study showed that red rice germ was able to decrease blood glucose levels and increase insulin resistance of DM rats and the strength of the pancreatic beta cells. ABSTRAKProses perkecambahan biji-bijian seperti beras, dapat meningkatkan beberapa nilai gizi seperti asam amino dan serat pangan. Beras merah dan kecambahnya diyakini mampu menurunkan glukosa darah pada penderita diabetes melitus (DM). Tujuan penelitian ini adalah mengevaluasi efek hipoglikemik kecambah beras merah pada tikus diabetes yang diinduksi STZ-NA terhadap kadar glukosa darah, insulin, serta indeks HOMA-IR dan HOMA β. Penelitian ini bersifat eksperimental in vivo pada hewan coba tikus Wistar jantan usia 2,5 bulan sebanyak 24 ekor dengan desain penelitian randomized post test only control group. Tikus dibagi menjadi 4 kelompok, masing-masing 1 kelompok tanpa induksi STZ-NA dengan diet standar dan 3 kelompok diinduksi STZ-NA dengan diet standar, beras merah dan kecambah beras merah. Percobaan dilakukan selama 6 minggu. Hasil penelitian menunjukkan kecambah beras merah mampu menurunkan kadar glukosa darah sebesar 61,88 % dan nilai HOMA-IR (parameter resistensi insulin) 56,82 %. Kadar insulin meningkat 16,35 % dan HOMA β 763,6 %. Disimpulkan, kecambah beras merah mampu menurunkan kadar glukosa darah dan memperbaiki kondisi resistensi insulin tikus DM, dan kekuatan sel beta pankreas.
Status Hematologi, Kadar IgG dan IgA Tikus yang Mengonsumsi berbagai Variasi Jumlah Tempe Kedelai Hitam Nurrahman Nurrahman; Mariyam Mariyam
agriTECH Vol 39, No 3 (2019)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (194.275 KB) | DOI: 10.22146/agritech.26118

Abstract

Consumption of black soybean tempe can provide rapid improvement in children with poor nutritional status and/or suffering from diarrhea. The antioxidant activity of black soybean tempe is higher than yellow soybean tempe. This study aimed to examine the effect of the amount of black soybean tempe in the feed on hematological status, IgG levels, and IgA levels of rat blood. There was a total of 6 groups, each containing 5 rats. The rats were kept in animal laboratories at room temperature (25–27°C) for 25 days. The six groups were induced Enteropathogenic Escherichia coli (EPEC) suspension with a concentration of 107 CFU / mL of 1 mL per day for seven days, orally using a feeding tube. Parameters analyzed included hematology status, IgG levels and IgA levels. The administration of black soybean tempe had an effect on increasing hemoglobin levels, the number of erythrocytes and the percentage of hematocrit, and it gave the effect of decreasing the number of leukocytes and platelets of rat blood induced with EPEC bacteria. The group of rats that consumed black soybean tempe had no influence on IgG and IgA levels of blood plasma.