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, Alimuddin

Department of Aquaculture

Author-ID : 480043

Agriculture, Biological Sciences & Forestry

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Sexual dimorphism related to growth in climbing perch Anabas testudineus Hidayat, Rahmat; Carman, Odang; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRCT The phenomenon that often occurs in climbing perch culture (Anabas testudineus, Bloch) is a significant size difference between individuals of male and female those lead to high variation in culture yields. In addition to genetic factors, the difference possibly reflects the sexual dimorphism in this fish; female grows faster than male. This research was conducted to examine sexual dimorphism related to growth quantitatively. Sixty individuals of 45-days old juvenile derived from mating of five pairs broods were randomly picked up and reared individually in 20×20×100 cm3 net cages for 135 days. Fish were fed on commercial feed (30% protein) three times a day at 10% feeding rate. Body weight and body length were individually recorded every 15 days, sex was determined at the end of the experiment by surgering the fish and gonad was weighed to calculate gonadosomatic index (GSI). The results showed that growth and specific growth rate of female were 1.17 and 1.48 fold respectively higher than male. This result indicates that female monosex culture of climbing perch is highly promising. Keyword: dimorphism, growth, climbing perch, Anabas testudineus ABSTRAK Salah satu fenomena yang sering dijumpai dalam budidaya ikan papuyu (Anabas testudineus, Bloch) adalah perbedaan ukuran yang mencolok antara individu jantan dan betina yang menyebabkan hasil panen ikan sangat bervariasi. Selain faktor genetik, perbedaan ini diduga disebabkan oleh adanya dimorfisme seksual terkait pertumbuhan. Penelitian ini dilakukan untuk menguji dimorfisme seksual terkait dengan pertumbuhan ikan papuyu secara kuantitatif. Benih ikan umur 45 hari hasil pemijahan lima pasang induk diambil secara acak sebanyak 60 ekor dan dipelihara selama 135 hari secara individu di dalam 60 unit hapa (20×20×100 cm3). Ikan diberi pakan komersial (protein 30%) tiga kali sehari sebanyak 10% bobot tubuh. Bobot dan panjang semua ikan dicatat setiap 15 hari sekali, jenis kelamin ditentukan di akhir penelitian dengan cara membedah, mengamati gonad, dan menimbangnya untuk menentukan indeks gonadosomatik (IGS). Hasil penelitian membuktikan adanya dimorfisme seksual terkait pertumbuhan pada ikan papuyu; pertumbuhan bobot mutlak dan laju pertumbuhan bobot harian ikan betina masing-masing 1,48 kali dan 1,17 kali lebih tinggi dibandingkan dengan ikan jantan. Hasil penelitian ini mengindikasikan bahwa pengembangan budidaya ikan papuyu monoseks betina prospektif untuk dilakukan. Kata kunci: dimorfisme, pertumbuhan, papuyu, Anabas testudineus

Digestive enzymes activities in Oreochromis niloticus fed diet supplemented with recombinant growth hormone Vinasyiam, Apriana; Suprayudi, Muhammad Agus; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 15 No. 1 (2016): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT The specific activity of the digestive enzymes, namely: pepsin, amylase, lipase, trypsin, and chymotrypsin were studied in Nile tilapia Oreochromis niloticus fed diet supplemented with recombinant Ephinephelus lanceolatus growth hormone (rElGH). The results showed that fish treated with rE1GH showed lower lipase and chymotrypsin specific activities (P>0.05), while the trypsin/chymotrypsin specific activity (T/C ratio) was found higher compared to control fish. Moreover, higher protein digestibility, higher protein retention and a lower ammonia excretion rate were measured for rE1GH treated fish. Oral rElGH administration enhanced Nile tilapia growth up to 20.04%, without affecting survival. This study suggested that rapid growth performance induced by rElGH was linked with T/C ratio rather than the specific activity of other digestive enzymes. Keywords: recombinant growth hormone, digestive enzyme, digestibility, Oreochromis niloticus ABSTRAK Aktivitas spesifik enzim pencernaan pepsin, amilase, lipase, tripsin, dan kemotripsin diamati pada ikan nila Oreochromis niloticus yang diberi pakan mengandung hormon pertumbuhan rekombinan ikan kerapu kertang Ephinephelus lanceolatus (rElGH)). Hasil menunjukkan bahwa ikan uji pada perlakuan rElGH memiliki aktivitas spesifik enzim lipase dan kemotripsin yang lebih rendah, sedangkan rasio tripsin/kemotripsin (rasio T/C) yang lebih tinggi dibandingan ikan kontrol. Kecernaan protein dan retensi protein bernilai lebih tinggi sementara laju ekskresi amonia bernilai lebih rendah pada ikan perlakuan. Pemberian rElGH secara oral mampu mempercepat laju pertumbuhan ikan nila hingga 20,04% tanpa memengaruhi kelangsungan hidup. Berdasarkan penelitian ini, dapat disimpulkan bahwa laju pertumbuhan cepat yang diinduksi oleh rElGH berhubungan dengan rasio T/C dibandingkan dengan aktivitas spesifik enzim pencernaan lain. Kata kunci: hormon pertumbuhan rekombinan, enzim pencernaan, kecernaan, Oreochromis niloticus

Performance of broodstock and hybrid juvenile of Egyptian and sangkuriang Clarias gariepinus strains Zulfania, Putri; Junior, Muhammad Zairin; Alimuddin, ,; Sunarma, Ade
Jurnal Akuakultur Indonesia Vol. 14 No. 2 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT This study was conducted to evaluate reproduction of broodstock and intraspecific hybrid juvenile performance of Egyptian (M) and sangkuriang (S) strain African catfish Clarias gariepinus at nursery phase. Intraspecific hybridization of African catfish was carried out reciprocally (SM and MS) and purebreed (SS and MM), each was with three replicates. Fish spawning was conducted by artificial fertilization, and larvae were reared at 1st, 2nd and 3rd nursery phases, subsequently. The results showed that broodstock performance (male and female) of both strains were not significantly different (P>0.05) on all reproduction traits, except female’s gonadosomatic index. Fertilization and hatching rates of all hybrids were not significantly different (P>0.05). MM juvenile had higher growth performances than other juvenile hybrids. Heterosis of total length, standard length, and body weight were varied, whereas the survival showed positive heterosis. SM juvenile showed positive growth heterosis in 3rd nursery phase (total length, standard length, and body weight were 2.61%; 2.16%, and 4.79% respectively). Survival heterosis of MS juvenile (24.20% for total length; 103.13% for standard length and 11.62% body weight) was higher than SM juvenile (6.86%; 48.57%, and 3.09%) on all nursery phases Keywords: African catfish, intraspecific hybridization, growth, survival, heterosis ABSTRAK Penelitian ini dilakukan untuk menguji performa reproduksi induk dan benih hasil hibridisasi intraspesifik ikan lele Afrika Clarias gariepinus strain Sangkuriang (S) dan Mesir (M) pada fase pendederan. Hibridisasi intraspesifik ikan lele Afrika dilakukan secara resiprokal (SM dan MS) dan galur murni (SS dan MM) masing-masing dengan tiga ulangan. Pemijahan dilakukan secara buatan dan larva yang dihasilkan dipelihara pada fase pendederan pertama, kedua, dan ketiga. Hasil penelitian menunjukkan bahwa performa induk ikan lele jantan dan betina pada kedua strain tidak berbeda nyata (P>0,05) pada seluruh parameter reproduksi, kecuali indeks gonadosomatik (GSI) pada induk betina. Derajat pembuahan dan penetasan telur pada seluruh persilangan tidak berbeda nyata (P>0,05). Pertumbuhan benih persilangan MM lebih tinggi, dibandingkan benih hasil persilangan lain. Nilai heterosis panjang total, panjang standar, dan bobot tubuh yang dihasilkan pada setiap stadia pendederan bervariasi, sedangkan nilai heterosis kelangsungan hidup menunjukkan hasil seluruhnya positif dibandingkan rataan galur murni. Heterosis pertumbuhan benih persilangan SM pada pendederan ketiga menunjukkan hasil yang positif (2,61% untuk panjang total; 2,16% untuk panjang standar dan 4,79% untuk bobot tubuh). Nilai heterosis kelangsungan hidup benih persilangan MS (24,20% untuk panjang total; 103,13% untuk panjang standar dan 11,62% untuk bobot tubuh) lebih tinggi dibandingkan benih persilangan SM (6,86%, 48,57% dan 3,09%) pada setiap stadia pendederan. Kata kunci: ikan lele Afrika, hibridisasi intraspesifik, pertumbuhan, kelangsungan hidup, heterosis

The sex ratio and testosterone levels in tilapia immersed in different doses of 17α-methyltestosterone Junior, Muhammad Zairin; Naufal, Muhammad Restya; Setiawati, Mia; Hardianto, Dian; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Tilapia fish farming using monosex male population has been reported to have 10% higher productivity compared to mix-sex culture. This study aimed to determine immersion dose of 17α-metiltestosterone (MT) that allowed higher male percentage, growth, survival, and measure testosterone level in fish body. The experiment was designed as three immersion doses, namely: 0; 1.8; and 5.4 mg/L MT, each with three replications. Immersion was conducted to 14-day-old larvae for four hours, with density of 100 fish/L of water. Testosterone levels in fish was measured using ELISA method, and sex identification was performed by histology method. The result showed that percentage of male fish was the same in the treatment of 1.8 mg/L and 5.4 mg/L, and 53–65% higher than the control without MT treatment (54% male). Growth and survival of fish until day 56 post immersion were the same. By ELISA analysis, the levels of testosterone in larvae just after immersion was similar in 1.8 mg/L and 5.4 mg/L treatments, decreased drastically on day 14 after immersion, and then the levels of testosterone to be similar with the control on day 28, i.e. about 2 ng/g. By PCR method with specific primer, sex reversed and normal males could be distinguished, and on day 71 the testosterone levels in sex reversed and normal males fish was also the same, 0.3 ng/g (P>0.05). Thus, sex reversal by immersion at a dose of 1.8 mg/L can be consider as a standard protocol for monosex tilapia production. Testosterone level in the body of MT-treated fish becomes the same to the control of less than one month post immersion

Aromatase gene expression and masculinization of Nile tilapia immersed in water 36 °C containing 17α-methyltestosterone Fauzan, Agung Luthfi; Soelistyowati, Dinar Tri; Junior, Muhammad Zairin; Hardiantho, Dian; Setiawati, Mia; Alimuddin, ,
Jurnal Akuakultur Indonesia Vol. 16 No. 1 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

ABSTRACT Immersion of undifferentiated larval tilapia in high temperature and 17α-methyltestosterone (MT) cab increase the male ratio. However, the effectiveness of immersion in high temperature of water containing MT remains to be evaluated. The purposes of this study were: 1) evaluate the male ratio, growth, and survival of tilapia, and 2) analyze the aromatase brain-type gene expression level in tilapia after immersing in high temperature (36 °C) containing MT at 2 mg/L for four hour with single and double immersion. Aromatase gene expression was analyzed by semi-quantitative RT-PCR (sqRT-PCR) method. The result showed that higher monosex male ratio was obtained by single immersion of MT at 36 °C at room temperature. Gene expression level of aromatase brain-type was lower on single immersion and increased significantly at second immersion compared to control (immersion at room temperature without MT). Immersion using MT and high temperature had no significant effect on fish survival. However the specific growth rate and fish biomass were higher than control. Thus, monosex male tilapia can be produced by single immersion of undifferentiated larvae at 36 °C temperature containing MT. Keywords: male ratio, aromatase, Oreochromis niloticus, temperature, 17α-methyltestosterone ABSTRAK Perendaman larva ikan nila yang belum terdeferensiasi kelaminnya dengan suhu tinggi dan hormon 17α-metiltestosteron (MT) dapat meningkatkan nisbah kelamin jantan. Tetapi, efektivitas perendaman menggunakan MT pada suhu tinggi belum diteliti. Tujuan dari penelitian ini adalah 1) mengevaluasi nisbah kelamin jantan, pertumbuhan, dan kelangsungan hidup ikan nila, dan 2) menganalisis ekspresi gen aromatase tipe-otak pada ikan direndam menggunakan MT dengan dosis 2 mg/L selama empat jam sebanyak satu dan dua kali perendaman pada suhu 36 °C. Ekspresi gen aromatase dianalisis menggunakan metode RT-PCR semi-kuantitatif (sqRT-PCR). Hasil penelitian menunjukkan bahwa kombinasi perendaman MT satu kali pada suhu 36 °C lebih tinggi menghasilkan ikan nila jantan monoseks dibandingkan perendaman MT satu kali pada suhu ruang. Tingkat ekspresi gen aromatase tipe otak pada perendaman satu kali lebih rendah, dan meningkat secara signifikan pada perendaman kedua dibandingkan dengan kontrol (perendaman pada suhu ruang tanpa MT). Perendaman larva menggunakan MT dan suhu 36 °C tidak berpengaruh nyata terhadap kelangsungan hidup, tetapi laju pertumbuhan spesifik dan biomassa ikan perlakuan tersebut lebih tinggi daripada kontrol. Dengan demikian, ikan nila jantan monoseks dapat diproduksi dengan perendaman satu kali pada larva yang belum terdeferensiasi jenis kelaminnya menggunakan MT pada suhu 36 °C. Kata kunci: rasio jantan, aromatase, Oreochromis niloticus, suhu, 17α-metiltestosteron

Co-Authors , Yuliyanti Ade Sunarma, Ade Agus Oman Sudrajat Antharest Sugati, Antharest Apriana Vinasyiam Ayu Dhita Juliadiningtyas, Ayu Dhita D. Irawan Dian Hardiantho Dian Hardianto Dinar Tri Soelistyowati Dita Puji Laksana Epro Barades Fauzan, Agung Luthfi Handika Gilang Pramana Putra Harton Arfah Julie Ekasari K. Sumantadinata La Ode Muhammad Arsal M. Zairin Junior Mia Setiawati MUHAMMAD AGUS SUPRAYUDI MUNTI YUHANA Naufal, Muhammad Restya Odang Carman Putri Zulfania, Putri Rahmat Hidayat Siti Subaidah Sri Nuryati Sri Setyo Wulandari, Sri Setyo Sumie Etoh Y. Hadiroseyani

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