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Effect of Serratia sp. Application Rate on Frost Damage, Growth, and Yield of Potato (Solanum tuberosum L.) Under Field Conditions Inggil Setyo Nugroho; Yuniel Melvanolo Zendrato; Maria Marina Herawati
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 15 No. 2 (2026): April 2026
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v15i2.821-831

Abstract

Frost damage poses a major constraint to potato (Solanum tuberosum L.) production in the Dieng Plateau, leading to significant yield loss. This study aimed to evaluate the effectiveness of Serratia sp. in mitigating the physical impacts of natural frost and improving plant growth and yield. The experiment was arranged in a Randomized Complete Block Design (RCBD) with four treatments of bacterial suspension application volume (density of 1 × 107 CFU/ml): control (0 ml/plant), 20 ml/plant, 40 ml/plant, and 60 ml/plant. A natural frost event occurred at a minimum temperature of -2 °C, causing minor damage. The results showed that the application of 60 ml/plant provided the best protection, with the lowest incidence of damage (<0.5%). The 40 and 60 ml/plant treatments consistently showed the highest plant height and leaf area, indicating accelerated vegetative recovery post-stress. In terms of yield, the 60 ml/plant treatment produced the highest tuber weight per plant (1,363 g). Further analysis confirmed that application volumes of 40 and 60 ml/plant significantly increased tuber size compared to the control. It was concluded that the application of Serratia sp. at a volume of 60 ml/plant was the best treatment in this study for stabilizing plant growth and maintaining potato productivity under low-temperature stress conditions in highland environments.
Pengaruh Penambahan Stroberi (Fragaria Sp.) terhadap Karakteristik Fisik dan Kimia Velva Pisang Susu (Musa acuminata var. silk) Intan Junita Lim; Maria Marina Herawati
JURNAL AGROINDUSTRI HALAL Vol. 10 No. 3 (2024): Jurnal Agroindustri Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v10i3.12903

Abstract

Pisang adalah komoditi yang menarik untuk dikembangkan dan ditingkatkan produksinya. Tingginya tingkat produksi pisang di Indonesia tidak sebanding dengan tingkat konsumsi masyarakat hal ini mengakibatkan banyaknya pisang yang kurang dimanfaatkan dengan baik. Namun perlu adanya diversifikasi pangan, yaitu dengan cara membuat olahan pangan berupa velva. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan nanas madu terhadap karakteristik fisik yaitu overrun, daya leleh, total padatan terlarut dan karakteristik kimia yaitu total antosianin dan vitamin C serta memperoleh perlakuan yang terbaik. Penelitian ini, menggunakan metode Rancangan Acak Kelompok (RAK) dengan 4 perlakuan dan 1 kontrol, setiap perlakuan dilakukan 5 kali pengulangan sehingga diperoleh 25 sampel. Adapun perlakuannya terdiri dari K0 Kontrol buah pisang susu 200 g tanpa penambahan stroberi, K1 (Buah pisang susu 175 g + buah stroberi 25 g), K2 (Buah pisang susu 150 g + dengan stroberi 50 g), K3 (Buah pisang susu 125 g + dengan stroberi 75 g), K4 (Buah pisang susu 100 g + dengan stroberi 100 g). Hasil dari penelitian ini semakin banyak penambahan buah stroberi berpengaruh terhadap hasil velva pisang susu dan stroberi pada overrun yang semakin rendah dan daya leleh semakin tinggi, dengan total padatan terlarut , total antosianin dan vitamin C menurun.
Karakteristik Kimia dan Sensori Crispy Cookies Kacang Hijau (Vigna radiata) dengan Penambahan Tepung Pisang Susu (Musa acuminata var. Silk) Puteri Anggrayni; Maria Marina Herawati
JURNAL AGROINDUSTRI HALAL Vol. 11 No. 1 (2025): Jurnal Agroindustri Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jah.v11i1.14041

Abstract

Crispy cookies are processed pastries that are usually made from wheat flour. Flour in making crispy cookies can use other alternatives, namely local food-based flour such as mung bean flour and milk banana flour. The researcher aims to determine the chemical and sensory characteristics and obtain the best treatments according to chemical analysis (analysis of water, carbohydrate, protein, ash, and fat content, E.coli contamination,) and organoleptic tests. This research used a Randomized Block Design (RAK) including 5 treatments and 5 replications. The five treatments had the following ratio of green bean flour to milk banana flour, P0 (100% : 0%), P1 (90%: 10%), P2 (80%: 20%), P3 (70%: 30%), and P4 (60%: 40%). Addition of milk banana flour has a significant effect on increasing water content, but does not have a substantial effect on protein, ash, fat, and carbohydrat content. The best treatment according to chemical analysis and organoleptic tests is P2 treatment.
Exploring anticancer activity of wild and polyploid mutant of Artemisia cina Sri Kasmiyati; Elizabeth Betty Elok Kristiani; Maria Marina Herawati; Ferdy S Rondonuwu
Biodiversitas Journal of Biological Diversity Vol. 22 No. 3 (2021)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d220319

Abstract

Abstract. Kasmiyati S, Kristiani EBE, Herawati MM, Rondonuwu FS. 2021. Exploring anticancer activity of wild and polyploid mutant of Artemisia cina. &nbsp;Biodiversitas 22: 1227-1234. The research aims were to explore the anticancer compounds in wild type (W) and polyploid mutant (P) of Artemisia cina Berg ex Poljakov using NIR and compare their cytotoxicity and selectivity on WiDR colon cancer and HTB183 lung cancer cell lines and Vero normal cell line. The W was obtained from B2P2TOOT Tawangmangu, Indonesia while P was by inducing A. cina shoot culture with 100 mg/l colchicine for 48 hours. They were extracted with hexane using the soxhletation method for 6 hours. The anticancer compounds were detected using NIR. The cytotoxic activity was determined using MTT assay, with Doxorubicin (D) as a control. The calculation of IC50 value used SPSS16 with a Probit analysis. The P contained the determined four compounds while the W contained no rutin. The IC50 values of W, P, and D were 295.5, 84.1, and 0.5 µg/mL on WiDr, 322.4, 128.6, and 39.9 µg/mL on HTB 183, whereas on Vero were 104, 315.6, and 295.5 µg/mL respectively. The selectivity indexes of W, P, and D were 34, 4, and 91 on WiDr, while HTB 183 was 31, 3, and 7, respectively. The P contained artemisinin, quercetin, kaemferol, and rutin, while the W contained no rutin. The cytotoxicity of both plants was less than doxorubicin. Both plants were selective on WiDR and HTB 183.
Potential of Gingerol in Emprit Ginger Extract (Zingiber officinale var. amarum) as a Biopesticide to Control Caterpillars (Crocidolomia pavonana) Tathe Surya Ananda; Maria Marina Herawati; Ruth Meike Jayanti
Journal of Global Sustainable Agriculture Vol 6, No 1 (April 2026)
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/jgsa.v6i1.1500

Abstract

Crocidolomia pavonana is a major pest that damages Brassicaceae plants such as cabbage, mustard greens, and broccoli. Continuous use of synthetic pesticides can cause pest resistance. This study aims to evaluate the acute toxicity and potential of Jahe Emprit (Zingiber officinale var. amarum) extract which is rich in gingerol as a botanical biopesticide against the third instar larvae of Crocidolomia pavonana, which is one of the main pests of Brassicaceae plants. This study was conducted using an experimental method with a Randomized Block Design (RBD) using four extract concentration treatments, namely 2%, 4%, 6%, and 8%, and used a negative control in the form of distilled water and a positive control pesticide Fenite 150 OD. Analysis of the active compound gingerol in the extract was carried out using High-Performance Liquid Chromatography (HPLC). The extract mortality test was carried out to determine Lethal Time 50 and Lethal Concentration 50 through the Topical Application method. The results of HPLC analysis of the bioactive gingerol compound showed a high level of 8.082 µg/mL. Mortality tests showed a significant increase in larval mortality along with increasing concentration and duration of exposure. The (LC50) value was recorded at 4.52%, indicating that the extract has a fairly high potential for acute toxicity. However, based on the (LT50) value of 60.26 hours (±2.5 days), this extract is included in the category of insecticides with a slow-acting mechanism, which is a typical characteristic of plant secondary metabolites. The most efficient extract concentration, namely 8%, was able to produce mortality of up to 70% and efficacy of 66.70% after 72 hours of treatment. The novelty of this research is the use of standardized ginger extract with verification of gingerol content through HPLC as a basis for biopesticide development., providing an alternative for sustainable pest control, and supporting environmentally friendly agricultural practices.
Carbon Emission Intensity and Bioactive Compound Quality in Organic vs. Conventional Emprit Ginger (Zingiber officinale var. amarum): A Life Cycle Assessment Approach Andreas Petra Wibiantoro; Maria Marina Herawati; Bistok Hasiholan Simanjuntak
Glosains: Jurnal Sains Global Indonesia Vol. 7 No. 3 (2026): Glosains: Jurnal Sains Global Indonesia
Publisher : Sekolah Tinggi Agama Islam Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59784/glosains.v7i3.762

Abstract

Background: Agricultural intensification drives environmental degradation, yet LCA studies often overlook carbon uptake and phytochemical quality. Objective: This study critically evaluates the trade-offs between environmental performance and functional quality in emprit ginger (Zingiber officinale var. amarum) cultivation under organic and conventional farming systems. While most previous studies emphasize greenhouse gas (GHG) emissions, this research integrates carbon footprint, carbon uptake proxies, and bioactive compound analysis within a unified framework using Life Cycle Assessment (LCA). Methods: A quantitative approach was employed combining cradle-to-grave LCA (ISO 14040 standard) with laboratory-based measurements of ash content and gingerol concentration. Primary data were obtained through field sampling and laboratory analysis, while secondary data were collected via structured farmer interviews to construct the life cycle inventory. Statistical differences between systems were assessed using independent t-tests. Results: The results demonstrate that organic cultivation significantly reduces carbon emission intensity (462.13 kg CO₂ ha⁻¹ ton⁻¹) compared to conventional practices (525.12 kg CO₂ ha⁻¹ ton⁻¹), primarily due to the exclusion of synthetic nitrogen fertilizers. Beyond environmental performance, organic systems produced significantly higher concentrations of gingerol (1.4068% w/w) and shogaol (0.0363% w/w), indicating enhanced secondary metabolism. Higher ash content in organic ginger further suggests improved mineral uptake and physiological activity associated with biomass carbon accumulation. Conclusion: These findings suggest that organic systems can simultaneously reduce emissions and improve product quality, supporting climate-smart agriculture and sustainable intensification in high-value horticultural crops.
Efficacy of Emprit Ginger Extract Containing Shogaol Compounds Armyworm (Spodoptera Litura) Larvae Yabez Yabez; Ruth Meike Jayanti; Maria Marina Herawati
Journal of Applied Agricultural Science and Technology Vol. 10 No. 3 (2026): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v10i3.573

Abstract

Spodoptera litura is a polyphagous pest that causes serious damage to various agricultural commodities. Control methods that still rely on synthetic pesticides negatively influence the environment and health, necessitating more environmentally friendly control alternatives. This study aims to determine the efficacy of ginger extract (Zingiber officinale var. amarum), which contains shogaol compounds, on the mortality of third instar S. litura larvae. The study was conducted experimentally using a one-factor completely randomized design (CRD) with six treatment levels: a positive control (fenite 150 OD), a negative control (Aquades), and ginger extract concentrations of 2%, 4%, 6%, and 8%, each with four replicates. Mortality tests were conducted using the topical application method and observed for 72 h. Data were analyzed using ANOVA, Tukey's test, and probit analysis to determine the LC₅₀, LC₉₀, LT₅₀, and LT₉₀ values. The results showed that ginger extract treatment significantly influenced the mortality of S. litura larvae (Sig. = 0.032). The 8% concentration resulted in the highest mortality of 82.5% at 72 h and the highest efficacy value of 82.5%, approaching that of the positive control. The LT₅₀ and LT₉₀ values were 37.15 hours and 64.81 hours, respectively, while the LC₅₀ and LC₉₀ values were 0.815% and 23.134%. The extract treatment also caused morphological changes in the larvae, including changes in body color, shrinkage, and tissue damage. Emprit ginger extract containing 6.8% shogaol, particularly at an 8% extract concentration, has the potential to serve as an effective environmentally friendly biopesticide in controlling S. litura.
In Vitro Efficacy of Gingerol-Containing Emprit Ginger (Zingiber officinale var. amarum) Extract Against Fusarium oxysporum Jane Tiara Maharani; Ruth Meike Jayanti; Sarawinda Hutagalung; Maria Marina Herawati
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 3 (2026): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i3.21261

Abstract

This study aimed to evaluate the antifungal activity of emprit ginger (Zingiber officinale var. amarum) extract containing 6-gingerol against Fusarium oxysporum under in vitro conditions and to determine the concentration with the highest inhibitory effect. The experiment was conducted using a completely randomized design with six treatments consisting of a negative control, emprit ginger extract at concentrations of 0.5%, 1.5%, 2.5%, and 5%, and a synthetic fungicide as the positive control, with five replications for each treatment. The 6-gingerol content was quantified using High-Performance Liquid Chromatography (HPLC), while antifungal activity was assessed using the poisoned food technique on Potato Dextrose Agar (PDA) medium. Data were analyzed using analysis of variance (ANOVA), followed by the Honestly Significant Difference (HSD) test at a 5% significance level. The results revealed that emprit ginger extract contained 6-gingerol at 8.082 µg/mL. Growth inhibition of F. oxysporum increased with increasing extract concentrations, reaching the highest inhibition (56.49%) at 5% extract concentration. Under the tested in vitro conditions, this treatment showed higher inhibition than the positive control (43.26%). The extract also induced morphological alterations, including reduced mycelial density, pigmentation changes, and restricted colony expansion. These findings indicate that emprit ginger extract containing 6-gingerol has potential as a botanical biofungicide candidate for suppressing F. oxysporum. Further validation through in vivo and field-scale studies is required before practical application.
Co-Authors Adella Natasha Alfred Jansen Sutrisno Andre Agusta Andreas Binar Aji Sukmana Andreas Petra Wibiantoro Andree Wijaya Setiawan Anggrayni, Puteri Armando Beresman Pangaribuan Ayu Valentina Ayu Valentina Aziz Purwantoro Aziz Purwantoro Bangkit Lutfiaji Syaefullah Bayu Nuswantara Bayu Setyaji Bistok Hasiholan Simanjuntak Catur Setiyani Chrismita B. Lusihanne Clement Panji Dinda Sukma Wibowo Djoko Murdono Dwi Haryono Dwi Septian, Bagas Dyan Yulianti Ebit Eko Bachtiar Elizabeth B.E Kristiani Elizabeth Betty Elok Kristiani Endang Pudjihartati Endang Sulistyaningsih Etimanta Pratama Sembiring Exvaricha Exvaricha Farid Maulana Ferdy Semuel Rondonuwu Fransisca Mareta Kurnia Sari Harefa, Thessalonika Elberta Hendrik Johannes Nadapdap Herry Susanto Inggil Setyo Nugroho Intan Junita Lim Ivan Tjahja Pranata Jane Tiara Maharani Jennifer Larisa Liem Jonathan Galih Raka Kusuma Kezia Natalia Kirani, Devi Kusuma, Jonathan Galih Raka Lestari, Dyah Aring Hepiana Lim, Intan Junita Liska Simamora Maria Maria Maria Maria Maria Melycorianda H. Ndapamuri Natalia, Kezia Natallie Whihel Mita Ndruru, Celvia Carlinawati Ni Putu Vidia Tiara Timur Ni Putu Vidia Tiara Timur Novinanto, Antonius Nugraheni Widyawati Purwoko, Agus Puteri Anggrayni Putri, Anindya N Rastosari A Ruth Meike Jayanti Sabrina Ayu Sarawinda Hutagalung Sari Setiyowati Septiandhani, Anugraheni Simanjuntak, istok Hasiholan Simarmata, Elvi Fitriani Siti Yulaekhah Sri Kasmiyati Suprayogi, Syahtian Suprihati Suprihati Suwijiyo Pramono Suwijiyo Pramono Syahtian Suprayogi Tathe Surya Ananda Theresa Dwi Kurnia Tinjung Mary Prihtanti Titaley, Naning Restiani Verrel Santosa Vincentia Irene Meitiniarti Yabez Yabez Yayi Suryo Prabandari Yoga Aji Handoko Yohanes Hendro Agus Yohanes Hendro Agus Yohanes Hendro Agus Yuliana Pratiwi Asti Yuliawati Yuliawati Yuniel Melvanolo Zendrato Zebrina Alfacesara Dewayanti