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DIRVAMENA BOER, DIRVAMENA
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Journal : Jurnal Agroteknos

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MIKROPROPAGASI PISANG MAS KIRANA (Musa acuminata L) MEMANFAATKAN BAP DAN NAA SECARA IN-VITRO Kasutjianingati, Kasutjianingati; Boer, Dirvamena
Jurnal Agroteknos Vol 3, No 1 (2013)
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The aim of this research was to study the effects of cytokinine and auxin (BAP and NAA) on micropropagation of banana cv Mas Kirana (AA).  The experiment used completely randomized design, with a single factor of multiplication media, consisted of 4 media i.e. MS + BAP 4 ppm,  MS + BA 6 ppm, MS + BAP 4 ppm + NAA 0,1 ppm,  MS + BA 6 ppm + 0.1 ppm. The experiment treatment of BAP 4 ppm produced the highest shoot number (100% small bud). The best shoot of Mas Kirana morphogenesis produced when the shoots sub-cultured in media MS0. The treatment 4 ppm BAP after sub-cultured at MS0 produced big plantlet ready to acclimatization for up to 43% (9 plantlets). Key words:   BAP, NAA, morphogenesis, big plantlet
SKRINING KETAHANAN BEBERAPA JENIS TANAMAN CABAI RAWIT TERHADAP CEKAMAN KEKERINGAN APRIZAL, MUHAMMAD; BOER, DIRVAMENA; HADINI, HAMIRUL; SADIMANTARA, I GUSTI RAY; MUHIDIN, MUHIDIN; ANIMA HISEIN, WAODE SITI
Jurnal Agroteknos Vol 13, No 1 (2023)
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The aim of this research is to obtain information on the resistance of several local cayenne pepper varieties from Southeast Sulawesi to drought stress. The study was conducted at Field Laboratory II, Faculty of Agriculture, Halu Oleo University, Kendari, which took place from January to June 2022. The research consisted of two experiments, namely the seed phase and the plant phase. The first experiment used a Factorial Randomized Block Design (RAK). The first factor was drought stress, consisting of 3 levels of PEG concentration (0, 7.5, and 15%). The second factor was the type of cayenne pepper, including 2 national varieties: Dewata and Bara, as well as 4 local cultivars from Konsel, Konawe, Muna, and Kendari. The second experiment also used a Factorial Randomized Block Design (RAK). The first factor was watering with field capacity (KL): 100, 50, and 25%. The second factor was the same as in the first experiment, which was the type of cayenne pepper. The first experimental variables were germination ability, germination weight, and drought stress index in the seed phase. The second experimental variables were plant height, number of leaves, root length, fruit weight, and stress tolerance index in the plant phase. Observational data were analyzed using variance analysis, followed by the BNJ test with a 95% confidence level. The results of the research showed that there was an interaction between the drought stress treatment and the type of cayenne pepper plant, which had a significant effect on plant height, root length, and fruit weight per plant. However, for germination ability, germination weight, and the number of leaves, each factor had an independent effect. With the 15% PEG treatment in the germination phase, the resistant cayenne pepper varieties were the cultivars from Muna and Konsel, while the 25% field capacity treatment for the plant phase of resistant cayenne pepper were Dewata and Bara varieties, cultivars from Muna and Konsel.
ANALISIS MORFOLOGI JAMBU METE (Anacardium occidentale L.) BERDASARKAN KARAKTER VEGETATIF DI TIGA KABUPATEN SULAWESI TENGGARA NAWIR, NAWIR; BOER, DIRVAMENA; LAREKENG, SITI HALIMAH; SUAIB, SUAIB; HADINI, HAMIRUL; HISEIN, WAODE SITI ANIMA; ARSYAD, MIRZA ARSIATY
Jurnal Agroteknos Vol 12, No 2 (2022)
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Abstract

Cashew is a promising plant in Southeast Sulawesi due to its reasonably high economic value. This species has been cultivated for years in Southeast Sulawesi, particularly in South Konawe, Konawe, and East Kolaka, with 20 to 30-years-old average plant age. This research aimed to identify morphological variations at the vegetative stage of the cashew in three evaluated Southeast Sulawesi populations (South Konawe, Konawe, and East Kolaka). The descriptive observations were carried out on the leaf apex shape, leaf shape, leaf margin, leaf venation, the color of young and mature leaves, branching pattern, tree habit, ease of peeling bark from the twig, leaf texture, as well as leaf aroma. We indicated the morphological variations in the evaluated cashew. The variations were observed in leaf shape (obovate, oblong, and circular), leaf apex shape (pointed, rounded, and indented), leaf venation (curved up, curved down, and flat), branching pattern (intensive and extensive), tree habit (upright and open, upright and compact, spreading), ease of peeling bark from twigs (difficult and easy), leaf texture (smooth and rough), and aroma (mango-like and guava-like).
ANALISIS VARIABILITAS GENETIK DAN KOEFISIEN LINTAS BERBAGAI KARAKTER AGRONOMI DAN FISIOLOGI TERHADAP HASIL BIJI DARI KERAGAMAN GENETIK 54 ASESI JAGUNG ASAL INDONESIA TIMUR Boer, Dirvamena
Jurnal Agroteknos Vol 1, No 1 (2011)
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The experiment was conducted to evaluate genetic variability, heritability, genetic gain, genetic and phenotypic correlation using path-coefficient analysis for some agronomic and physiology characters of 54 accessions of maize from East Indonesia.  The experiment was  arranged in  a  randomized completely block  design  with  tree  replications using  54 accesions.   Characters observed were plant high/TTM, number of leaves/JDT, number of leaves  above  ear/JDA,  Length  of  branches  part  of  tassel/PTM, length  of  peduncle/PMA, length of ear/PTO, diameter of ear/DTO, number of kernel rows per ear/JBT, flowering time of  male  flower/UBJ,  flowering  time  of  female  flower/UBB,  maturity  time/UMT,  seed size/UBJ, leaf area index/ILD, and seed yield/BBJ.   The result of the experiment indicated that genetic variability, broadsense heritability and genetic gain for all agronomic and phisiology characters were high.  Maize seed yield improvement can be effectively done by selecting for number of leaves, maturity time and seed size. Keywords:    Genetic variability, heritability, correlation, path-coefficient analysis
HUBUNGAN KEKERABATAN AKSESI PISANG KEPOK (Musa paradisiaca Formatypica) DI KABUPATEN MUNA BERDASARKAN KARAKTER MORFOLOGI DAN PENANDA RAPD Wijayanto, Teguh; Boer, Dirvamena; Ente, La
Jurnal Agroteknos Vol 3, No 3 (2013)
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Twenty-four accessions that belong to four groups of kepok banana in Muna Regency have   been   analyzed   for   their   genetic   diversity   based   on   morphological   characters (qualitative and quantitative characters), and a few accessions based on RAPD markers. This study aimed to determine the genetic diversity and phylogenetic relationship of accessions of kepok bananas based on 52 qualitative and 12 quantitative morphological characteristics and DNA characteristics.   Results of clustering analysis showed the euclidian values ranged between 0.50 to 1.00 for the qualitative data, 0.01 to 0.50 for quantitative data, and 0.83 to 0.88  for  DNA  profile  data.  Combined  qualitative  and  quantitative  data  had  similarity coefficient ranged from 0.00 to 2.50.  Dendogram of each character produced 2 main groups. The main group 1  formed subgroups. Although the qualitative and quantitative characters resulted in different accession   groupings, the combined data analysis of quantitative and qualitative  data  showed  that kepok  banana  in  Muna  regency  was  classified into    4  sub groups namely banana Manuru, Bugisi, Jiwaka and Manuru Lakabu. Keywords:   cluster   analysis,   kepok   banana,   qualitative   and   quantitative   characters, morphology, RAPD markers.
KETAHANAN SUMBERDAYA GENETIK JAGUNG SULAWESI TENGGARA TERHADAP CEKAMAN KEKERINGAN PADA BERBAGAI FASE VEGETATIF WIJAYANTO, TEGUH; GINTING, CANDRA; BOER, DIRVAMENA; AFU, WA ODE
Jurnal Agroteknos Vol 4, No 2 (2014)
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Abstract

Maize crops experiencing water stress can experience cell damage, loss of turgor, closed stomata, plant leaf roll then wilt.  Germination and vegetative growth are thought to be a very sensitive phases in relation to the availability of water, because it can influence subsequent growth processes. This study aimed to determine the potential tolerance of Southeast Sulawesi’s maize genotypes to drought stress at different vegetative growth phases. This study was based on completely randomized design (CRD) with factorial pattern consisting of two factors: the first factor composed of 9 local maize genotypes of Southeast Sulawesi and 1 national variety (cv. Arjuna), while the second factor was drought stress at different vegetative growth phases, consisting of four levels ie:  C0 = plants irrigated with 100 % water availability during the growth phase, C1 = Stress for 5 days , at 21-26 days old (vegetative phase), C2 = Stress for 5 days starting at panicle emergence (early flowering stage), and C3 = Stress for 5 days starting 2 weeks after silking.  Research results showed that Genotype (G) treatment significantly influenced all observed growth variables (at age 21 and 42 days after planting, DAP), except for the variable of number of leaf, age 21 DAP. However, water stress treatment (C) only significantly affected plant height variable, at the age of 42 DAP.   In general, G6 and G7 genotypes tended to have a higher crop and trunk diameter than the other genotypes.  Contrary, G3 genotype tended to have shorter crop and smaller stem diameter than the other genotypes.  There are indications that the drought stress treatment (C) significantly inhibited the growth of maize crops. Keywords: drought strees, maize genotypes, tolerance, and vegetative phases  
ANALISIS VARIABILITAS KULTIVAR JAGUNG PULUT (Zea mays Ceritina Kulesh) LOKAL SULAWESI TENGGARA SAFUAN, LA ODE; BOER, DIRVAMENA; WIJAYANTO, TEGUH; SUSANTI, NELI
Jurnal Agroteknos Vol 4, No 2 (2014)
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Abstract

The experiment was conducted in the Rahandouna village, Poasia, Kendari Southeast Sulawesi, from August to November 2013.  The purpose of this study was to determine the estimate of heritability between characters of thirteen local waxy corn cultivars of Southeast Sulawesi. This study was prepared using a randomized block design (RBD), with 3 replicates. Total waxy corn cultivars studied was 13 species, so that there were 39 plots. Each plot consisted of a single cultivar. Observed variables were plant height (cm), stem diameter (cm), leaf area (cm2), number of leaves (strands), ear length (cm), cob diameter (cm), number of rows per ear, weight of 100 seeds (g), ear weight. The results of this study showed that there was narrow variability on all local waxy corn characters observed. Keywords: Local waxy corn, characters, cultivars, Southeast Sulawesi, variability
INDUKSI TUNAS PISANG KEPOK (Musa paradisiaca L.) MENGGUNAKAN KOMBINASI KINETIN DAN IAA SECARA IN VITRO BOER, DIRVAMENA; PRAWANSA, AGUNG; RAKIAN, TRESJIA CORINA; ARSYAD, MIRZA ARSIATY; ARIF, NORMA; MADIKI, ABDUL; ARSANA, I MADE WISANA
Jurnal Agroteknos Vol 14, No 2 (2024)
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This study aims to investigate the effect of combining Kinetin and IAA on the growth of kepok banana shoots in vitro. The research was conducted at the In Vitro Laboratory, Agrotechnology Unit, Faculty of Agriculture, Halu Oleo University, from January to April 2023. The study utilized a Completely Randomized Design (CRD) with six treatments: P1 = (Kinetin 1 mg L⁻¹ + IAA 2 mg L⁻¹), P2 = (Kinetin 3 mg L⁻¹ + IAA 4 mg L⁻¹), P3 = (Kinetin 5 mg L⁻¹ + IAA 6 mg L⁻¹), P4 = (Kinetin 2 mg L⁻¹ + IAA 1 mg L⁻¹), P5 = (Kinetin 4 mg L⁻¹ + IAA 3 mg L⁻¹), and P6 = (Kinetin 6 mg L⁻¹ + IAA 5 mg L⁻¹). Each treatment had five replicates, resulting in 30 experimental units, with four Kepok banana shoot explants per culture bottle. The observed variables included shoot emergence time, number of shoots, shoot length, and shoot growth percentage. Results indicated that the combination of Kinetin and IAA concentrations significantly affected the number of shoots and shoot emergence time and shoot length, but did not significantly affect the shoot growth percentage. The best treatment was P2 = (Kinetin 3 mg L⁻¹ + IAA 4 mg L⁻¹) for in vitro growth of Kepok banana shoots.
PERBANYAKAN BIBIT JERUK SECARA IN-VITRO DAN BEBAS PENYAKIT CVPD Wijayanto, Teguh; Taufik, Muhammad; Boer, Dirvamena; Suliartini, Ni Wayan Sri
Jurnal Agroteknos Vol 1, No 3 (2011)
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Abstract Citrus Vein  Phloem Degeneration (CVPD) is  one  of  the  most important diseases of citrus. Worldwide and national yield losses of citrus production due to this disease infection have been very significant.   This research ultimately aimed at producing citrus stocks through in-vitro culture, and free of CVPD.  Citrus seeds were used as explant and cultured on MS and WPM in-vitro media, supplemented with malt extract. Citrus plantlets were tested for the presence of  CVPD DNA using CVPD specific primers in PCR reactions.   Research results showed that MS basal medium supplemented with malt extract was quite good for in- vitro production of citrus plantlets. Plantlets were negative for CVPD infection based on PCR tests.  CVPD-free seedlings (Citrus reticulata) have been grafted with citrus rootstock (Citrus sinensis).  Grafted citrus seedlings were also proven to be negative for CVPD infection based on similar PCR tests.  Citrus seedlings/stocks produced by in-vitro culture, and free of CVPD, are now available for further growth. Keywords: Citrus Vein Phloem Degeneration (CVPD), in-vitro culture, PCR
KERAGAMAN GENETIK BEBERAPA CABAI RAWIT (Capsicum frutescens L.) LOKAL ASAL SULAWESI TENGGARA SUDIARSIH, TIRA; BOER, DIRVAMENA; HADINI, HAMIRUL; SUTARIATI, GUSTI AYU KADE; ANIMA HISEIN, WAODE SITI; WIJAYANTO, TEGUH
Jurnal Agroteknos Vol 13, No 3 (2023)
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This study aims to determine the genetic diversity of several local cayenne pepper cultivars from Southeast Sulawesi and to find out which local cayenne pepper cultivars from Southeast Sulawesi show the best growth and yields. This research was carried out at the Agricultural Extension Center (BPP) Pilot Land located in Langkoroni Village, Maligano District, Muna Regency. The research implementation time starts from October 2021 to February 2022. Data analysis using variety fingerprints, if it shows a real and very real influence then continued Duncan multiple range test (DMRT) with the help of statistical product and service solution (SPSS) application facility and calculate genetic diversity value. The results showed that quantitative characters with high diversity were found in the characters of flowering age, harvest age, number of fruits, fruit weight, fruit length and fruit diameter. Somewhat high is found in the character of plant height and leaf area. Slightly low was found in the character of the number of leaves and low was found in the character of the length of the fruit stalk. The qualitative characters observed were uniform because there were no differences in the observed local cayenne pepper cultivars from Southeast Sulawesi. The local cayenne pepper cultivar from Southeast Sulawesi that showed the highest growth and fruit yield was found in the cultivar from Kolaka (145.96 grams/plant), followed by North Buton-2 (125.71 grams/plant), then South Konawe (121.93 grams/plant) compared to other local cayenne pepper cultivars.
Co-Authors Abda Abda Abdul Madiki ANDI NURMAS, ANDI ANIMA HISEIN, WAODE SITI APRIZAL, MUHAMMAD Arief, Norma ARSANA, I MADE WISANA Arsyad, Mirza Arsiaty Asrianingsi, Asrianingsi Asrul Sani Awaluddin, Andi Cahyani, Imas Nur Candra Ginting Cornelio Gago Eka Febrianti, Eka Ente, La Febrianti Feriatin, Feriatin Gusti Ayu Kade Sutariati Hisein, Waode Siti Anima Jamili Jamili Kasutjianingati Kasutjianingati, Kasutjianingati Kilowasid, Muhammad Harjoni La Karimuna La Ode Safuan Makmur Jaya Arma Muhammad Aldi, Muhammad MUHAMMAD TAUFIK Muhidin Muhidin Muhidin Muhidin Muzuni, Muzuni Nawir Nawir, Nawir NELI SUSANTI, NELI Nini Mila Rahni NORMA ARIF, NORMA Nur Arfa Yanti Nuraida, Wa Ode Pasolon, Yulius Bara PRAWANSA, AGUNG Rachmawati Hasid Robiatul Adawiyah SADIMANTARA, I GUSTI RAY SARAWA MAMMA, SARAWA Sarawa, Sarawa SITI HALIMAH LAREKENG, SITI HALIMAH Slamet, Agustono Sri Ambardini Suaib Suaib, Suaib SUDIARSIH, TIRA Suleman, Darwis Suliartini, Ni Wayan Sri SYAMSU ALAM Teguh Wijayanto TRESJIA CORINA RAKIAN, TRESJIA CORINA WA ODE AFU, WA ODE Warhamni, Warhamni