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Pengaruh Waktu Sentrifugasi Pada Sexing Spermatozoa Dengan Media Bovine Serum Albumin Terhadap Membran Plasma Utuh Spermatozoa X-Y Sapi Simmental Priyanto, Langgeng; Putranti, Oktora Dwi; Riswandi, Riswandi; Gunawan, Muhammad; Susanda, Apriansyah; Setianingsih, Eva
Cannarium Vol 20, No 2 (2022)
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/cannarium.v20i2.5290

Abstract

Penelitian ini bertujuan untuk mendapatkan waktu sentrifugasi yang tepat pada sexing spermatozoa dengan media Bovine Serum Albumin terhadap membran plasma utuh spermatozoa  X-Y pada sapi Simmental. Penelitian ini dilaksanakan Oktober 2021 sampai dengan November 2021 di Laboratorium Reproduksi dan Kesehatan Ternak Balai Pembibitan Hijauan Pakan Ternak Sembawa. Penelitian ini menggunakan rancangan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan (P1: waktu sentrifugasi 4 menit; P2: waktu sentrifugasi 8 menit; dan P3: waktu sentrifugasi 12 menit) dan 4 ulangan. Parameter yang diamati adalah membran plasma utuh spermatozoa X dan Y. Hasil menunjukan bahwa perlakuan waktu sentrifugasi berpengaruh nyata terhadap membran plasma utuh spermatozoa pada spermatozoa X dan Y (P0,05).  Hasil penggunaan waktu yang tepat yaitu pada P1 dengan waktu sentrifugasi 4 menit dimana nilai lapisan atas membran plasma utuh X semen sexing yaitu sebesar 62,93%, lapisan bawah Y sebesar 63,94%. Penelitian ini bertujuan untuk mendapatkan waktu sentrifugasi yang tepat pada sexing spermatozoa dengan media Bovine Serum Albumin terhadap membran plasma utuh spermatozoa  X-Y pada sapi Simmental. Penelitian ini dilaksanakan Oktober 2021 sampai dengan November 2021 di Laboratorium Reproduksi dan Kesehatan Ternak Balai Pembibitan Hijauan Pakan Ternak Sembawa. Penelitian ini menggunakan rancangan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 4 ulangan. Parameter yang diamati adalah membran plasma utuh spermatozoa X dan Y. Hasil menunjukan bahwa membran plasma utuh spermatozoa pada spermatozoa X dan Y berpengaruh nyata (P0,05) terhadap perlakuan waktu sentrifugasi.  [D1] Hasil penggunaan waktu yang tepat yaitu pada P1[D2]  dengan waktu sentrifugasi 4 menit dimana nilai lapisan atas membran plasma utuh X semen sexing yaitu sebesar 62,93%, lapisan bawah Y sebesar 63,94%.  [D1]Bukan membran plasma yang berpengaruh nyata terhadap perlakuan waktu sentrifugasi, namun sesuai judul, perlakuan waktu sentrifugasi yang berpengaruh nyata terhadap membran plasma [D2]Perlu dijelaskan terlebih dahulu definisi P1, P2, P3 di dalam abstrak
Edukasi dan Aplikasi Diet Fitobiotik Kunyit pada Ikan Lele Budidaya Sistem Bioflok di Masyarakat Sekolah Tahfidz Khoiru Ummah Palembang Wijayanti, M; D. Wulandari; M. Amin; M. Nursan; L. Priyanto; C.A. Safitra; E. Triah; M.A.H. Pratama
Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat Vol. 6 No. 2 (2026): Maret 2026 - Jurnal Altifani Penelitian dan Pengabdian kepada Masyarakat
Publisher : Indonesian Scientific Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59395/altifani.v6i2.1057

Abstract

Kebutuhan masyarakat di sekitar Sekolah Tahfidz Khoiru Ummah Plus (STPKU) pengelola budidaya ikan adalah inovasi teknologi perikanan berkelanjutan. Minat masyarakat terhadap budidaya ikan sebagai sumber pendapatan tambahan untuk meningkatkan pendapatan keluarga harus diimbangi dengan risiko kematian ikan yang signifikan, yang menjadi kendala bagi pengembangan usaha perikanan. Permasalahan dalam manajemen budidaya ikan, terutama pada saat cuaca yang tidak menentu, dapat menurunkan kesehatan dan produksi ikan. Solusi yang diberikan adalah dengan memberikan edukasi dan pendampingan sistem budidaya ikan menggunakan bioflok, dengan aplikasi pakan fitobiotik dan pemuasaan untuk meningkatkan efisiensi produksi. Metode yang digunakan adalah praktik fitobiotik-bioflok bagi mitra dan masyarakat sekitar, dimulai dengan edukasi dan pelatihan teknologi inovatif budidaya ikan menggunakan sistem bioflok dan pakan fitobiotik, berlanjut melalui tahap pendampingan hingga panen, dan termasuk tahap monitoring dan evaluasi keberlanjutan. Budidaya ikan lele dengan sistem bioflok rawa dan penambahan bubuk kunyit pada pakan sebagai fitobiotik memberikan dampak yang lebih besar terhadap efisiensi pakan, pertumbuhan, dan kelangsungan hidup. Kegiatan pengabdian kepada masyarakat yang dipadukan dengan praktik kerja lapangan mahasiswa dinilai bermanfaat bagi masyarakat, meningkatkan pemahaman dan minat terhadap budidaya ikan fitobiotik.
Different effects of swamp probiotics application frequency as a biofloc-forming agent on the production of catfish (Clarias gariepinus) Mohamad Amin; Retno Cahya Mukti; Ferdinand Hukama Taqwa; Andini Andini; Marsi Marsi; Langgeng Priyanto; Marini Wijayanti
Depik Vol 13, No 2 (2024): AUGUST 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.2.34280

Abstract

Catfish (Clarias gariepinus) that are reared with probiotics as biofloc-forming agent is thought to increase the fish production. Applying swamp probiotics to the water media has never been studied to ensure the flocks' availability in the rearing media. This study aimed to determine the appropriate frequency of probiotics application collected from swamps for biofloc formation to improve the catfish production. This study used a completely randomized design with two treatments and three replications. The treatments were composed of different application frequency of swamp probiotics: (P1) once in 42 days of rearing and (P2) twice in 42 days of rearing. Data on flock volume, total bacterial colonies, absolute growth rate, feed efficiency, survival rate, and water quality were analyzed by T-test with a 95% confidence level. Meanwhile, the flock composition data were analyzed descriptively. The results showed that P2 obtained the best treatment with a floc volume of 68.33 10.41 mL/L, absolute length growth of 8.18 1.03 cm, absolute weight growth of 19.30 3.12 g, feed efficiency of 135.24 7.98%, survival rate of 89.33 6.21%, biomass production of 24639.50 1344.51 g, temperature of 28.85-29.59C, pH of 7.27-7.42, dissolved oxygen (DO) of 3.91-5.72 mg/L, ammonia of 0.45-1.15 mg/L, and total dissolved solids (TDS) of 717.33-885.50 mg/L. Therefore, swamp probiotics should be applied to catfish culture media twice for 42 days of rearing or once every 21 days.Keywords:BioflocCatfishProbiotics from swamp
Different effects of swamp probiotics application frequency as a biofloc-forming agent on the production of catfish (Clarias gariepinus) Mohamad Amin; Retno Cahya Mukti; Ferdinand Hukama Taqwa; Andini Andini; Marsi Marsi; Langgeng Priyanto; Marini Wijayanti
Depik Vol 13, No 2 (2024): AUGUST 2024
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.13.2.34280

Abstract

Catfish (Clarias gariepinus) that are reared with probiotics as biofloc-forming agent is thought to increase the fish production. Applying swamp probiotics to the water media has never been studied to ensure the flocks' availability in the rearing media. This study aimed to determine the appropriate frequency of probiotics application collected from swamps for biofloc formation to improve the catfish production. This study used a completely randomized design with two treatments and three replications. The treatments were composed of different application frequency of swamp probiotics: (P1) once in 42 days of rearing and (P2) twice in 42 days of rearing. Data on flock volume, total bacterial colonies, absolute growth rate, feed efficiency, survival rate, and water quality were analyzed by T-test with a 95% confidence level. Meanwhile, the flock composition data were analyzed descriptively. The results showed that P2 obtained the best treatment with a floc volume of 68.33 10.41 mL/L, absolute length growth of 8.18 1.03 cm, absolute weight growth of 19.30 3.12 g, feed efficiency of 135.24 7.98%, survival rate of 89.33 6.21%, biomass production of 24639.50 1344.51 g, temperature of 28.85-29.59C, pH of 7.27-7.42, dissolved oxygen (DO) of 3.91-5.72 mg/L, ammonia of 0.45-1.15 mg/L, and total dissolved solids (TDS) of 717.33-885.50 mg/L. Therefore, swamp probiotics should be applied to catfish culture media twice for 42 days of rearing or once every 21 days.Keywords:BioflocCatfishProbiotics from swamp
Increase in Immunoglobulin Y (IgY) Levels and Modification of Duck Egg Yolk Cholesterol Composition through Feed Additives Eli Sahara; Langgeng Priyanto; Meisji Liana Sari
JURNAL ILMIAH PETERNAKAN TERPADU Vol. 14 No. 3 (2026): Jurnal Ilmiah Peternakan Terpadu
Publisher : DEPARTMENT OF ANIMAL HUSBANDRY, FACULTY OF AGRICULTURE, UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jipt.v14i3.p836-849

Abstract

This study aimed to analyze the effect of supplementation of a combination of turmeric powder, black pepper, and pyridoxine in the diet on production performance, physical egg quality, and the levels of Immunoglobulin Y (IgY) and total cholesterol in the egg yolk of ducks. The study used a Completely Randomized Design with 7 treatments and 4 replications, each consisting of 3 Mojosari ducks aged 5 months and reared for 7 weeks. The treatments were R0 (basal ration), R1 (BR + pyridoxine 3 mg/kg), R2 (BR + pyridoxine 6 mg/kg), R3 (BR + turmeric 2.4 g/kg + black pepper 0.2 g/kg + pyridoxine 3 mg/kg), R4 (BR + turmeric 2.4 g/kg + black pepper 0.2 g/kg + pyridoxine 6 mg/kg), R5 (BR + turmeric 2.4 g/kg + black pepper 0.6 g/kg + pyridoxine 3 mg/kg), and R6 (BR + turmeric 2.4 g/kg + black pepper 0.6 g/kg + pyridoxine 6 mg/kg). Production performance and physical egg quality data were analyzed using ANOVA followed by Duncan’s multiple range test, while IgY and cholesterol data were analyzed descriptively. The results showed that the treatments had no significant effect (P>0.05) on feed consumption, egg production, or Hen Day Production. Eggshell weight in treatment R3 (5.26 g) was significantly lower (P<0.05) than that of the control (7.24 g). Based on descriptive analysis, there was a numerical increasing trend in IgY and total yolk cholesterol levels as the supplementation level increased, with the highest values observed in R6 (IgY: 157.27 mg/mL; cholesterol: 1136.7 mg/100 g). In conclusion, this combination supplementation did not disturb production performance in general but reduced eggshell weight and indicated an increase in IgY and total yolk cholesterol levels that warrants further investigation.