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Journal : JURNAL BIOLOGI INDONESIA

 1 
Variasi Intraspesies Lactobacillus plantarum (Orla-Jensen) Bergey et al. Asal Sayur Asin Berdasarkan Analisis Molekuler ., Sulistiani; ., Abinawanto; Sukara, Endang; Dinoto, Achmad; Mangunwardoyo, Wibowo
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2162

Abstract

The current study is the first report on intraspecies analysis of L. plantarum from sayur asin in Indonesia using molecular approach. Three molecular techniques, i.e., restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and enterobacterial repetitive intergenic consensus (ERIC-PCR) were used to determine the intraspecies diversity of L. plantarum responsible for spontaneous fermentation in sayur asin. These methods were aimed to discriminate 46 isolates of L. plantarum isolated from sayur asin, including the type strain. PCR amplification of the 16S-23S rDNA ISR revealed two-bands profile of 800 and 600 bp specific to lactobacilli. RAPD-PCR and ERIC-PCR were very valuable in discriminating genetic polymorphism among L. plantarum isolates by producing bands ranged from 4-10 bands (360-2620 bp) and 6-12 bands (160-2900 bp), respectively. Dendograms generated from UPGMA cluster analysis based on RAPD-PCR and ERIC-PCR data showed that all isolates were grouped into three major clusters with 74% and 68.6% genetic similarity thresholds, respectively.The study indicated that strains belong to L. plantarum isolated from sayur asin were divided into three genotypic groups. Keywords: ERIC-PCR, Intraspecies, Lactobacillus plantarum, RAPD-PCR, RFLP 16S-23S rDNA ISR 
VARIASI INTRASPESIES LACTOBACILLUS PLANTARUM (ORLA-JENSEN) BERGEY ET AL. ASAL SAYUR ASIN BERDASARKAN ANALISIS MOLEKULER ., Sulistiani; ., Abinawanto; Sukara, Endang; Dinoto, Achmad; Mangunwardoyo, Wibowo
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2162

Abstract

The current study is the first report on intraspecies analysis of L. plantarum from sayur asin in Indonesia using molecular approach. Three molecular techniques, i.e., restriction fragment length polymorphism (RFLP) 16S-23S rDNA intergenic spacer region (ISR), random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) and enterobacterial repetitive intergenic consensus (ERIC-PCR) were used to determine the intraspecies diversity of L. plantarum responsible for spontaneous fermentation in sayur asin. These methods were aimed to discriminate 46 isolates of L. plantarum isolated from sayur asin, including the type strain. PCR amplification of the 16S-23S rDNA ISR revealed two-bands profile of 800 and 600 bp specific to lactobacilli. RAPD-PCR and ERIC-PCR were very valuable in discriminating genetic polymorphism among L. plantarum isolates by producing bands ranged from 4-10 bands (360-2620 bp) and 6-12 bands (160-2900 bp), respectively. Dendograms generated from UPGMA cluster analysis based on RAPD-PCR and ERIC-PCR data showed that all isolates were grouped into three major clusters with 74% and 68.6% genetic similarity thresholds, respectively.The study indicated that strains belong to L. plantarum isolated from sayur asin were divided into three genotypic groups. Keywords: ERIC-PCR, Intraspecies, Lactobacillus plantarum, RAPD-PCR, RFLP 16S-23S rDNA ISR 
Co-Authors Abd. Rasyid Syamsuri Abinawanto Abinawanto Achmad Dinoto Achmad Dinoto Andi Hartono Andi Salamah Atit Kanti Atit Kanti Bernadeta Ayu Widyaningrum Dede Hermawan Dolly Priatna Ernawati Sinaga Evi Triana Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi Febia Rini Hanies Ambarsari Hastuti, Ratih Dwi Herman Suparman Simanjuntak Jajang Sutiawan KATSUHIKO ANDO Kusumah, Sukma Surya Latifah K Darusman latifah K Darusman Latifah K. Darusman Lisdayanti, Puspita Lukman Hakim Miranti Nurindah Sari MISA OTOGURO Nampiah Sukarno NITA ROSALINDA PRAYITNO Noverita Nuniek Ina Ratnaningtyas Nuraeni Ekowati Purity Sabila Ajiningrum Puspita Lisdayanti PUSPITA LISDIYANTI Puspita Lisdiyanti Puspita Lisdiyanti Ragamustari, Safendrri K. Ragamustari, Safendrri Komara Rohmatussolihat - ROHMATUSSOLIHAT ROHMATUSSOLIHAT Rohmatussolihat Rohmatussolihat Ruth Meliawati Ruth Melliawati Ruth Melliawati Shanti Ratnakomala SHANTI RATNAKOMALA Solly Aryza Sri Lestari Subkhan Subkhan Sukma Surya Kusumah Sulistiani ., Sulistiani Sulistiani Sulistiani Sulistiani Sulistiani, Sulistiani Sutiawan, Jajang Trisanti Anindyawati Trisanti Anindyawati, Trisanti WIBOWO MANGUNWARDOYO Wibowo Mangunwardoyo Widyaningrum, Bernadeta Ayu Widyastuti, Anisa Y. Purwanto Yantyati Widyastuti Yantyati Widyastuti Yantyati Widyastuti Yopi Yopi YOPI YOPI YULIN LESTARI Yulneriwarni Yulneriwarni