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All Journal Jurnal Fitopatologi Indonesia Planta Tropika Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Caraka Tani: Journal of Sustainable Agriculture Jurnal Keperawatan Widya Gantari Indonesia
Ani Widiastuti, Ani
Fakultas Pertanian Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Health Professions Medicine & Pharmacology

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Induced Resistance of Shallot (Allium cepa L. var aggregatum) against Twisted Disease Using Ultraviolet-B Light Ahmad, Yan Syahrial; Wibowo, Arif; Widiastuti, Ani
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.79701

Abstract

Ultraviolet-B (UV-B) light is known to induce plant resistance in suppressing disease infection. Its ability to induce plant resistance has been widely used as an inducer of plant resistance. This study aims to determine the ability of UV-B light in suppressing shallot twisted disease, potency of plant resistance induction through accumulation of peroxidase enzymes, salicylic acid, chlorophyl content and to observe shallot growth after treatment. This study applied UV-B light with a duration of 65 mW/m2 for 4 hours each day for 3, 5 and 7 days, for inoculated and non-inoculated plants. Fungicide (Benomyl) was used as a comparison for inoculated plants. The results showed that UV-B irradiation of 4 hours/day for 7 days was able to reduce the incidence of shallot twisted disease, but the incubation period among treatments was not significantly different. UV-B irradiation of 4 hours/day, for 7 days was able to increase salicylic acid content, and maintain the same chlorophyll content as the negative control, however the peroxidase enzyme content was not significantly different. Treatment of UV-B 4 hours/day for 7 days irradiation did not inhibit the growth and production of shallot plants.
Penyakit Busuk Akar dan Pangkal Batang Tebu di Sumatera Selatan Maryono, Tri; Widiastuti, Ani; Priyatmojo, Achmadi
Jurnal Fitopatologi Indonesia Vol. 13 No. 2 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (604.321 KB) | DOI: 10.14692/jfi.13.2.67-71

Abstract

Root and basal stem rot disease caused by Xylaria sp. is a new diseases in Indonesian sugarcane plantation. The disease has been reported only occurred in sugarcane plantations in Sumatera. Limited information about the disease has slowed down disease diagnosis in the field. The research was aimed to describe symptoms, signs, and spatial distribution of the disease caused by Xylaria. A survey was conducted at a sugarcane plantation in South Sumatera (3° 26’ 16.6” S dan 104°40’ 09.8” E). Typical symptoms of the disease involved drying of all leaves and plant death, dry rot of root and basal stem, and retarded growth of ratoon cane were recorded. Stroma was found on surface of diseased stems and on soil surface of diseased plants, and spatial aggregation of diseased plants was observed in all locations. The distribution of the diseased plant aggregately and the presence of stroma on diseased plants are important characteristics of the disease diagnosis in sugarcane plantations.
Maize induced resistance against downy mildew mediated by benzoic acid increased photosynthesis rate and chlorophyl content Habibullah, Muhammad; Joko, Tri; Wibowo, Arif; Widiastuti, Ani
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 25 No. 2 (2025): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLAN
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.225255-261

Abstract

Maize downy mildew, caused by Peronosclerospora spp., is a major disease affecting maize cultivation in tropical regions, particularly in Indonesia. One potential control strategy is the induction of plant resistance. Previous studies have demonstrated that benzoic acid (BA), a non-fungicidal chemical compound, can induce maize resistance against downy mildew. Induced resistance mechanisms do not directly inhibit pathogen development but enhance the plant’s defensive response to infection. This study utilized various maize lines, ranging from susceptible to resistant, to evaluate the impact of BA-induced resistance on photosynthesis rate and chlorophyll content as the key agronomic parameters associated with the plant growth. The results showed that BA treatment did not significantly increase the photosynthetic rate and chlorophyll content in moderately resistant and resistant maize lines. However, in susceptible and moderately susceptible lines, BA treatment enhanced both parameters, indicating its role in strengthening plant resistance. These findings suggest that resistant maize lines do not respond to BA induction, whereas susceptible and moderately susceptible lines benefit significantly from its application. This provides new insight that BA can potentially induce resistance in susceptible maize plants against downy mildew.
PENGALAMAN PASIEN GAGAL JANTUNG DI RSJPD HARAPAN KITA TERHADAP PERAWATAN DIRINYA DI RUMAH: STUDI FENOMENOLOGI Widiastuti, Ani; Nurachmah, Elly; Sekarsari, Rita; Budi, Indra
Jurnal Keperawatan Widya Gantari Indonesia Vol 7 No 2 (2023): JURNAL KEPERAWATAN WIDYA GANTARI INDONESIA (JKWGI)
Publisher : Nursing Department, Faculty of Health, Universitas Pembangunan Nasional "Veteran" Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52020/jkwgi.v7i2.5789

Abstract

Gagal jantung menjadi masalah kesehatan yang progresif dengan angka mortalitas dan morbiditas yang tinggi di negara maju maupun negara berkembang. Tingginya angka readmission juga menyebabkan tingginya biaya perawatan yang harus dikeluarkan, oleh karena itu diperlukan pendekatan penanganan yang baik dengan meningkatkan efektifitas perawatan diri di rumah. Penelitian ini bertujuan untuk mengeksplorasi pemahaman yang mendalam tentang pengalaman, kebutuhan dan harapan pasien gagal jantung dalam melaksanakan perawatan dirinya (self care) di rumah. Penelitian ini menggunakan desain penelitian deskriptif kualitatif dengan pendekatan fenomenologi. Pemilihan partisipan diambil dengan cara purposive sampling sebanyak delapan orang. Pengumpulan data dilakukan dengan wawancara mendalam dengan membuat pertanyaan berdasarkan tujuan yang ingin dicapai. Data yang diperoleh dianalisis dengan menggunakan langkah-langkah Colaizzi sehingga dapat disimpulkan tema-tema sesuai pengalaman partisipan. Dari hasil analisa data ditemukan dua belas tema utama yaitu : (1) pengetahuan gagal jantung (2) Tanda dan gejala yang dialami (3) respon terhadap penyakit (4) mengatur pola makan (5) mengkonsumsi obat (6) olah raga dan aktifitas (7) kontrol ke dokter (8) hambatan yang dihadapi (9) dukungan keluarga (10) dukungan informasi (11) sumber informasi (12) harapan pasien. Melalui penelitian ini, kebutuhan pasien, kesulitan yang dihadapi serta harapan terhadap perawatan dirinya dapat teridentifikasi dengan jelas. Pasien gagal jantung yang melakukan perawatan diri di rumah membutuhkan dukungan keluarga serta dukungan informasi untuk dapat menjalankan program pengobatan dengan baik. Melalui penelitian ini dapat direkomendasikan untuk disusun media edukasi dan informasi yang dapat memudahkan pasien gagal jantung dalam melakukan perawatan dirinya di rumah sehingga harapan pasien untuk dapat ditangani dengan baik dapat terlaksana.
Deciphering the signature of seedborne fungi linked to rice sheath rot disease: insights from ITS rDNA sequencing analysis Hoerussalam, Hoerussalam; Afifuddin, Ahmad; Lathif, Yudrik; Widiastuti, Ani; Putra, Eka Tarwaca Susila; Priyatmojo, Achmadi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 26 No. 1 (2026): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.126165-179

Abstract

Sarocladium oryzae and Fusarium spp. are the causal agents of sheath rot, a re-emerging rice disease that has recently gained importance in Indonesia and can cause yield losses of up to 85%. Both pathogens are seedborne, making their accurate identification and management essential. Conventional morphological identification is time-consuming and often inaccurate due to overlapping symptoms among fungal species. In this study, we demonstrated the seedborne transmission of sheath rot pathogens and provided novel insights by highlighting the predominance of F. equiseti and the detection of infections in asymptomatic seeds. A total of 75 fungal isolates were obtained from rice leaf sheaths, seeds, and harvested grains across CMS, inbred, and hybrid rice varieties. ITS rDNA sequencing identified 42 isolates as S. oryzae and 33 as Fusarium spp., including F. equiseti (29), F. incarnatum (1), and F. proliferatum (3). The detection of these pathogens in both pre-planting seed samples and post-harvest grains demonstrates their ability to spread through seeds. Importantly, their presence in asymptomatic seeds and grains indicates that routine visual inspection is insufficient for seed health monitoring.
Identification of Pathogens Causing Important Diseases in Leatherleaf Fern (Rumohra adiantiformis) and In Vitro Inhibition using Bacillus velezensis B-27 Tsaniyah, Barokati; Joko, Tri; Widiastuti, Ani
Caraka Tani: Journal of Sustainable Agriculture Vol 39, No 2 (2024): October
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v39i2.83675

Abstract

Leatherleaf fern (Rumohra adiantiformis) is a famous ornamental-leaf plant that has been used by florist entrepreneurs around the world. It is one of the leading export commodities in Indonesia, however, currently, there are some diseases of this leaf reported in the field causing yield loss and reducing the economic value. This study aimed to identify the pathogens causing the recent 3 significant diseases of leatherleaf fern, including leaf blight, leaf tip rot, and post-harvest leaf rot, and in vitro analysis of beneficial bacteria, Bacillus velezensis B-27, against the pathogens. The methods used in this study were isolation, pathogenicity test, morphological observation, molecular identification of pathogens, and poisoned food technique of B. velezensis against those pathogens compared to fungicides and bactericides. The results of molecular identification showed that Neopestalotiopsis sp. and Pantoea ananatis caused leaf blight, Fusarium oxysporum f. sp. sesami triggered leaf tip rot, while Calonectria sp. and P. ananatis contributed to post-harvest leaf rot. Based on in vitro analysis, B. velezensis B-27 reduced the growth of the Neopestalotiopsis sp. DM C with the highest inhibition of 95.6%, Neopestalotiopsis sp. DM B with 84.3%, F. oxysporum f. sp. sesami with 61.9%, Calonectria sp. with 93.4%, and inhibited the growth of P. ananatis by producing a clear zone. This research concludes that B. velezensis B-27 has the potential as a biocontrol against pathogens causing significant diseases in leatherleaf ferns due to its ability to inhibit pathogens and its advantage as a beneficial microbe that is environmentally friendly to support sustainable agriculture.
Tomato Mutants SlIAA9 Exhibit Thermo-Morphophysiological Characters and Enhanced SIDREBA4 Gene Expression Rafsyanyani, Cory; Anjalani, Titah Rigel; Rahayu, Anisa Esti; Mubarok, Syariful; Widiastuti, Ani; Matra, Deden Derajat; Ezura, Hiroshi; Jadid, Nurul
Caraka Tani: Journal of Sustainable Agriculture Vol 41, No 1 (2026): January
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/carakatani.v41i1.108173

Abstract

Rising temperatures associated with climate change threaten tomato productivity, yet the contribution of auxin signaling components to heat-stress adaptation remains incompletely understood. The IAA9 gene, encoding an Aux/IAA transcriptional repressor, is well known for its role in auxin-regulated development, but its role in heat responses is still unclear. This study aims to elucidate the function of IAA9 in modulating tomato responses under heat stress conditions. Researchers utilized tomato iaa9-3 and iaa9-5 mutants and exposed them to prolonged elevated temperatures of 40 to 45 °C for 6 weeks to assess morphophysiological traits, and to 38 to 40 °C for 6 days to evaluate molecular responses through SlDREBA4 gene expression analysis. Under prolonged heat stress, all genotypes exhibited reduced leaf area, leaf number, and total chlorophyll content, accompanied by increased plant height compared to plants grown under normal conditions. Specifically, wild-type Micro-Tom (WT-MT) showed the lowest values in leaf area (165.89 cm²), leaf number (23 leaves), and total chlorophyll content (115.7 µg g-1). In contrast, the iaa9-3 and iaa9-5 mutants recorded the highest plant heights at 11.98 and 12.13 cm, respectively, indicating a differential growth response under stress. Gene expression analysis revealed that SlDREBA4 expression was upregulated in both iaa9-3 and iaa9-5 mutants compared to normal temperature conditions, with increases of 0.45-fold and 1.78-fold, respectively. These results indicate that IAA9 mutations confer enhanced thermotolerance in tomato, as reflected by altered morphology and increased heat-responsive gene expression. This study highlights IAA9 as a potential genetic target for improving heat stress resilience in tomato breeding programs.
Co-Authors Achmadi Priyatmojo Ahmad Afifuddin Ahmad, Yan Syahrial Alifia Nur Ayusma Anjalani, Titah Rigel Arif Wibowo Arlyna Budi Pustika Ayundai, Melin Budi, Indra Christanti Sumardiyono Danar Wicaksono, Danar Eka Tarwaca Susila Putra Elly Nurachmah Ezura, Hiroshi Ganies Riza Aristya Harper, Stephen Hoerussalam, Hoerussalam Karmila, Miratun Lathif, Yudrik Matra, Deden Derajat Muhammad Habibullah Nurul Jadid Pratiwi, Annisa Hasta Putri, Fauzana Rafsyanyani, Cory Rahayu, Anisa Esti Rahelly, Yetty Rina Sri Kasiamdari Santika, Islaminati Anna Sayfdaningsih, Sayfdaningsih Sekarsari, Rita Siti Subandiyah Sundari, Dini Suryanti Syafitri, Luthfiana Mifta Syariful Mubarok Tri Joko Tri Maryono Triwidodo Arwiyanto Tsaniyah, Barokati Werdiningtyas, Cyrilla Kinanti Wibowo, Eko Prasetyo