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All Journal HAYATI Journal of Biosciences SAINS DAN MATEMATIKA Jurnal Ilmu Dasar Jurnal Sumberdaya Hayati BioEksakta : Jurnal Ilmiah Biologi Unsoed Makara Journal of Science
Jepri Agung Priyanto, Jepri Agung
Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Immunology & microbiology Mathematics Veterinary

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Deteksi Bakteri Gram-Negatif Pada Permukaan Daun Kemangi (Ocimum basilicum) dari Tiga Pasar Tradisional di Bogor: Detection of Gram-Negative Bacteria on the Leaf Surface of Basil (Ocimum basilicum) Collected from Three Traditional Markets in Bogor Getriani, Zerina; Priyanto, Jepri Agung; Budiarti, Sri
Jurnal Sumberdaya Hayati Vol. 9 No. 4 (2023): 2023
Publisher : Departemen Biologi, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jsdh.9.4.138-144

Abstract

Ocimum basilicum or basil is commonly consumed raw by Indonesian. However, it is easily contaminated by pathogenic bacteria. This study aimed to detect Gram-negative bacteria in basil leaves obtained from three traditional markets in Bogor. Isolation and quantification of bacteria using serial dilution showed that the average number of bacteria on the surface of basil leaves was 9.6 × 107 CFU/g. Basil leaves obtained from the second traditional market had the highest number of bacteria of 11.3 × 107 CFU/g. All isolated bacteria have the same morphology namely bacilli, then further purified and characterized physiologically. The nine bacterial isolates obtained were able to grow on the selective-differential media including Salmonella-Shigella agar (SSA), bismuth sulphite agar (BSA), and eosin-methylen blue (EMBA). Gram staining showed that all bacterial isolates were classified as Gram-negative bacteria. The hemolytic ability of bacterial isolates was tested using blood agar base media, three out of nine bacterial isolates were able to produce hemolysin. Molecular identification based on 16S rRNA sequences showed that the bacterial isolates belonged to the Providencia sp., Proteus sp., Pseudomonas sp., and Kluyvera sp.
Co-Authors Alzahra, Ziqri Dwi Retnowati Getriani, Zerina Hasidu, La Ode Abdul Fajar Hening, Egiyanti Nur Widhia Ilsan, Noor Andryan Inggrain, Maulin Ivan Permana Putra Khairani, Hafifatunil Maherani, Vincentia Fenice Angger Melda Yunita MG Isworo Rukmi Nabilla, Yasmin Nuha NISA RACHMANIA MUBARIK Nuraeni, Siti Panesya Permatasari, Vera Prastya, Muhammad Eka Primahana, Gian Ramanda, Viqih Rhesi Kristiana Riswanti, Mellyana Rizna Triana Dewi Septianingrum, Danik Simbolon, Sumihartati Siti Nurfajriah Sri Budiarti Poerwanto Sri Pujiyanto Suryanti, Erma Sutandi, Michellia Salmaa Ashilla Ulfa, Aulia Wibowo, Muhammad Arya