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All Journal Jurnal Riset Gizi Jurnal Analis Kesehatan Journal of Maternal and Child Health Jaringan Laboratorium Medis MIMBAR INTEGRITAS Jurnal Riset Gizi Jurnal Media Analis Kesehatan Jurnal Riset Gizi JHE (Journal of Health Education)
Djoko Priyatno, Djoko
Health Analysts Department, Polytechnic Health Ministry of Semarang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Jaringan Laboratorium Medis

 1 
Kepatuhan Pemeriksaan PPIA (Pencegahan Penularan HIV dari Ibu Ke Anak) Ibu Hamil dengan Risiko HIV (Human Immunodeficiency Virus) Wibowo, Anang; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 1 No. 1 (2019): May 2019
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v1i1.5164

Abstract

Human Immunodeficiency Virus (HIV) is an infectious disease with a death rate and the number of disease incidence is high and requires the diagnosis and the therapy long enough. The success of the program depends on the PPIA socialization of HIV and Syphilis in pregnant women, as well as compliance checked her pregnancy during pregnancy for early detection of HIV and Syphilis that is useful for the treatment of early and proper while the results the examination showed reactive. The purpose of this research to know description of compliance inspection of PPIA (prevention of HIV transmission from mother to child) of pregnant women with HIV risk in public health II in Baturraden. The method is observational approach time crosssectional tests. Population in the study are all pregnant women and pregnant women throughout the research sample of trimester I, timester II, and trimester III who programme PPIA in public health II in Baturraden of this year in 2018 as many as 100 people. The research results obtained compliance examination of the PPIA pregnant women HIV risk i.e. age 35-44 in 76%; secondary education diploma 100%; and employment status of civil servant or the student as much as 100%. While 55% of disobedience at 15-24 years old; elementary school level 73%; and a housewife 59%. The higher the education, age, and occupation of a person then the PPIA program to carry out compliance higher.
Prevalensi Hasil Pemeriksaan HBsAg dan Anti HBs pada PSK (Pekerja Seks Komersial) Priyatno, Djoko; Qomariyah, Nurul
Jaringan Laboratorium Medis Vol. 1 No. 2 (2019): November 2019
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v1i2.5453

Abstract

Commercial sex workers are a high risk group of STD infection (HIV-AIDS, syphilis, gonorrhea and Hepatitis-B). Transmission of HBV 100x faster than the HIV. Diagnosis HBsAg and Anti-HBs is a marker of HBV infection can be detected at least 2 weeks before clinical symptoms appear. The incidence of STIs Semarang (in 2012) is the highest in the resocialization Argorejo as much as 1,623 cases. Monitoring of Hepatitis B in resocialization has not become a routine examination. The formulation of the research problem is "How prevalence of HBsAg and Anti-HBs on resocialization PSK Argorejo Semarang?". This study is observational analytic cross-sectional design. Specimens research is venous blood PSK Argorejo resocialization who check monitoring of HIV-AIDS in August-September 2015.  Sampling a total of sampling for examination imunoserologis HBsAg and Anti-HBs in the strip method. Analysis of the relationship between host factors with HBsAg and anti-HBs using Spearman's test. Negative values on the results of HBsAg while the percentage of anti-HBs PSK resocialization some 29%. There is no relationship between age, education and knowledge with Anti-HBs. There is a relationship between sexual behavior with Anti-HBs. Upregulation required screening and immunization of Hepatitis-B infection in the community resocialization Argorejo Semarang.
Studi Literatur Gambaran C-Reactive Protein Pada Penderita Hipertensi Fadilah, Umi; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 2 No. 2 (2020): November 2020
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v2i2.7696

Abstract

Hypertension is called the silent killer, often known after complications occur. Based on the results of Riskesdas in 2018, the prevalence of hypertension in Indonesia at age ≥18 years increased to 34.1% in 2018. Endothelial injury caused by hypertension triggers inflammation or chronic inflammation of the arterial wall. Evidence of an inflammatory process is the discovery of C-Reactive Protein (CRP) which is a marker of inflammation from non-bacterial infections.The purpose of this study to knowing the description of C-Reactive Protein in patients with hypertension through research by Fadhliani (2016), Dolly (2018), Harahap (2019). This paper is a summary of  research result, with the criteria for systematic study research through a systematic review approach. In patients with hypertension according to research Fadhliani (2016) obtained as much as 23% C-Reactive Protein positive and 77% C-Reactive Protein negative. In the Harahap study (2019) of 30 people with hypertension, 12 people (40%) had positive C-Reactive Protein and 18 people (60%) had negative C-Reactive Protein. Whereas in the study of Dolly (2018) the average level of hs-CRP in hypertensive patients is 4, 85 mg / L. According to Dolly's research (2018) in patients with hypertension for > 1 year have higher levels of hs-CRP compared to patients with hypertension for a period of ≤1 years. Examination of C-Reactive Protein (CRP) in patients with hypertension in the Harahap study (2019) showed positive CRP results dominated by subjects with age ≥ 50 years as many as 8 people (27%).
Histologi Jaringan Hepar Mencit (Mus Musculus) yang Difiksasi dengan Larutan Carnoy dengan Variasi Waktu 4 Jam, 8 Jam dan 12 Jam Afrida, Arin Dwi; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 3 No. 1 (2021): May 2021
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v3i1.7920

Abstract

Histopathological examination is a routine examination process for each tissue in the anatomic pathology laboratory. The stages in making histology preparations are fixation stages. The type of solution that can be used as a fixative solution other than 10% NBF is Carnoy's solution. Carnoy's solution is a fixative solution with a relatively fast fixation process of about 1-4 hours. The advantages of Carnoy's solution can lyse erythrocytes and dissolve lipids, have the ability to maintain cell nuclei, and retain glycogen. The purpose of this study was to determine the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using Carnoy's solution with time variations of 4 hours, 8 hours, and 12 hours. This is an observational research with descriptive research design criteria. The results of the histology of the liver tissue preparations of mice (Mus Musculus) which were fixed using the 10% NBF solution group obtained 100% good preparations. In the Carnoy solution group with a time of 4 hours, it gave 2.2% results of poor preparations and 97.78% of good preparations. While the treatment group with Carnoy's solution with a time of 8 hours obtained as many as 11.11% of preparations that were not good and 88.89% of good preparations. and in the Carnoy solution group with a time of 12 hours, there were 44.44% of the poor preparations and 55.56% of the good preparations.
Minyak Zaitun Sebagai Pengganti Xylene pada Prosesing Jaringan Histologis untuk Pewarnaan Kulit dan Hepar Mencit dengan Hematoxylin Eosin: Sebuah Studi Perbandingan Sofyanita, Eko Naning; Iswara, Arya; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 4 No. 2 (2022): November 2022
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v4i2.8688

Abstract

Clearing results in histological processing can be affected due to tissue density and viscosity of the clearing agent. Xylene is the most commonly used aromatic solvent for clearing agents and deparaffinizing agents in histopathology laboratories, but xylene is one of the most dangerous chemicals found in histology laboratories. Based on the dangers posed by xylene, a replacement material is needed. Several xylene substitutes such as reagents derived from limonene, aliphatic hydrocarbons, vegetable oils and mineral oils have been commercially developed, but the available xylene substitutes are still less effective, more expensive, and still as dangerous as xylene itself. Substitutes that are considered safer are from natural oils, one of which is olive oil which has similarities with xylene, namely in hydrocarbon and phenol compounds. This study used two types of tissue, namely skin and liver from mice (Mus musculus) which were cut into two parts; the first part uses xylene as and the other part uses olive oil as a clearing agent. The assessment of hematoxylin eosin staining results were determined based on the category of assessment of cell structure, such as the cell nucleus, cytoplasm, and color uniformity carried out by three readers and five fields of view for each microscopic slide at 40X magnification of the objective lens. Comparison of observations of the xylene group in skin and liver tissue 100% got a good score on the cell nucleus, cytoplasm, and color uniformity. (p=1,000). The olive oil group had a slight difference in color uniformity in liver tissue when compared to skin, but not statistically different (p=0.773). The comparison of the overall readings of the xylene and olive oil groups on skin and liver tissue also showed no statistically different (p=0.262). So it can be concluded that olive oil can be recommended as a substitute for xylene in the clearing process in histological tissue processing.
Hubungan Antara Kadar Asam Urat dengan Kepatuhan Minum Obat pada Pasien Tuberculosis Paru Auliya, Qurrotu A'yuni; Maulana, Mochamad Rizal; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 5 No. 2 (2023): November 2023
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v5i2.9545

Abstract

Tuberculosis lungs is disease infectious disease caused by bacteria Mycobacterium tuberculosis. TB disease still become threat health Indonesian society which leads to illness , disability and death tall so that need exists countermeasures. Research objectives know connection between rate sour tendon with obedience drink medication in pulmonary TB patients. Study This is study retrospective analytic observational with approach cross sectional, with use analysis Research Chi square test statistics conducted at the Ambarawa Community Health Center (BALKESMAS) in October 2022. Population all moderate pulmonary TB patients undergo treatment at BALKESMAS Ambarawa. Research sample This of the total population namely 30 patients. Results from 30 respondents there is rate sour veins in patients Tuberculosis lungs there is enhancement rate sour tendon namely 11 respondents that is man with average levels sour urate 7.08 mg/dL (6 respondents ), female with average level 6.41 mg/dL (5 respondents ). Based on forever drink drug obtained that amount patient with treatment >3 months 57%, 2-3 months 30%, and 1 month 13%. Based on characteristics that kind sex men 53% and kind sex women 47%. Based on characteristics age category teenagers 23%, adults 50% elderly 27%. Based on obedience drink anti-tuberculosis drugs by 28 respondents or 93.34% of total number of respondents . Whereas For respondents who did not obedient drink drug namely 2 respondents or 6.06%. Chi square test results on compliance status drink medicine, type gender, age No showing connection significant (P<0.05) against rate sour veins in patients Tuberculosis lungs. Research conclusions This No There is connection rate sour tendon with obedience drink medication in pulmonary TB patients, This caused by TB Treatment consists of over 2 stages that is intensive and advanced, function system immunity body relate with age, history of medication, obesity, consumption purines , and alcohol.
Efektivitas Masa Penyimpanan Filtrat Daun Jati (Tectona grandis) terhadap Kualitas Pewarna Preparat Telur STH (Soil Transmitted Helminth) Rahmadhani, Afiffah Gita; Afrianti, Dina; Priyatno, Djoko
Jaringan Laboratorium Medis Vol. 7 No. 2 (2025): November 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i2.13018

Abstract

Soil-Transmitted Helminth (STH) infections such as Ascaris lumbricoides remain a public health concern in Indonesia, necessitating microscopic fecal examinations using high-quality stains. Eosin is commonly used but poses environmental and health risks. Anthocyanins found in teak leaf (Tectona grandis) filtrate offer a safer and more eco-friendly alternative. One factor affecting anthocyanin color stability is storage duration. This study aimed to evaluate the effectiveness of teak leaf filtrate stored for 0, 7, 14, and 21 days on the staining quality of STH egg preparations. This quasi-experimental study used a Posttest-Only Nonequivalent Control Group Design. The samples consisted of 24 Ascaris lumbricoides egg preparations divided into four treatment groups with six replications each. Staining quality was assessed by three validators based on contrast under the microscope, color absorption, and morphological clarity. Data were analyzed using the Kruskal-Wallis test to determine differences in staining quality across storage durations. All preparations showed good staining quality, and no significant differences were found between the storage groups (p-value = 0.383; > 0.05). These results indicate that teak leaf filtrate remains effective for staining STH egg preparations for  up to 21 days of storage, with the best results observed at 7 days after preparation. Further research is recommended to explore this topic more deeply, with attention to laboratory procedures and technical skills.
Co-Authors Afrida, Arin Dwi Ahmad Thohir Hidayat Amirul Baldi, Wildan Anung Sugihantono Arya Iswara Auliya, Qurrotu A'yuni Birahmatika, Fitya Safira Dina Afrianti1 Fauzia, Khalida Helda Khusun Istiqomah, Anisa Judhiastuty Februhartanty Kuncara, Rachmad Bayu Maulana, Mochamad Rizal Muchtaruddin Mansyur Nurlita, Hera Nurul Qomariyah Pradina Rahmawati, Pradina Pramesthi, Indriya Laras Rahmadhani, Afiffah Gita Rodhi Hartono Sofyanita, Eko Naning Sri Noor Mintarsih Surati Surati Teguh Budiharjo, Teguh Umi Fadilah, Umi Wibowo, Anang Widodo Widodo