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Microbial Protein Synthesis by Cellulolytic Bacterial Isolates from Feces of Indonesian Endemic Herbivores Pramartaa, Ikhsan Qodri; Wiryawan, Komang G.; Suharti, Sri
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 2 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i2.509

Abstract

Cellulolytic bacterial play an important role in the synthesis of microbial protein in the small intestine as high-quality protein. This research aimed to observe the growth patterns of cellulolytic bacterial isolates from the feces of endemic herbivorous animals in Indonesia, and to evaluate the effect of the addition of cellulolytic bacterial isolates on the synthesis of microbial proteins in vitro. The study used a completely randomized design with four treatments and five replications. The treatments included T0 = Concentrate mix 40%: elephant grass 30%: palm leaves 30% (control), T1 = T0 + 105 CFU mL-1 cellulolytic bacterial isolate, T2 = T0 + 106 CFU mL-1 cellulolytic bacterial isolate, and T3 = T0 + 107 CFU mL-1 cellulolytic bacterial isolate. Variables measured were the growth pattern of bacterial isolates, bacterial population at the optimal growth point, and microbial protein synthesis. The results showed growth pattern of cellulolytic bacterial isolates from Muntjac, Deer and Anoa feces have similar pattern, and was different from the growth pattern of bacterial isolates from bison feces. The optimal growth point of cellulolytic bacterial isolates from the feces of Muntjac, Deer, and Anoa at the 8th hour of incubation with bacterial populations of 1.3 x 109 CFU mL-1, 1.7 x 109 CFU mL-1, and 1.4 x 109 CFU mL-1. The growth pattern and optimal point of cellulolytic bacterial isolates from the feces of Bison at the 12th hour after incubation with bacterial populations of 1,1 x 1010 CFU mL-1. The addition of cellulolytic bacterial isolates did not affect microbial protein synthesis.
Risk Mitigation of Oyster Mushroom Production and Distribution Using Method Failure Mode and Effect Analysis (FMEA) Miftah, Himmatul; Rapigah, Siti; Mubarokah, Syaima Lailatul; Masithoh, Siti; Kusumawati, Riny; Pramartaa, Ikhsan Qodri
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 3 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i3.748

Abstract

The production process of making raw materials for horticultural commodities has various risks, both internal and external, and if it occurs, it will hinder the company's goals. This research aims to analyse and measure risks in the production and distribution of oyster mushrooms and determine actions to mitigate risks. The research method used purposive and snowball sampling. Data were analysed using descriptive analysis through interviews and quantitatively through risk analysis and Failure Mode and Effect Analysis (FMEA) methods. The study results indicated that risks occur from the nursery stage to distribution to consumers. Risk mapping that needs to be watched out for: Mucor spp disease, baglogs attacked by crepe pests, damaged baglogs in the incubation section, and incorrect and irregular watering and fertilization. The risks in distribution analysed are the risk of heavy losses, physical damage, and unsold goods. The most significant risks in the distribution process were heavy losses and physical damage to mushrooms at the farmer level during the sorting process, and physical damage at the retailer level during the sorting process due to the presence of old, wet, and yellowing mushrooms, as well as unsold mushrooms. Production is mitigated by cleaning around the mushroom house, burning diseased baglogs, arranging baglog entry and exit times to facilitate the sorting process, and implementing routine work direction and evaluation. Mitigation of unsold risks during distribution: making a purchasing plan according to previous sales results, selling remaining mushrooms at a lower price than the purchase price, and using PBP and VBP packaging.
Effectiveness of Diets Incorporating Nano-Zeolite and Urea-Impregnated Nano-Zeolite on Fermentation Efficiency in Rumen Fluid In Vitro Kardaya, Dede; Dewi, Pia Nurmala; Sudrajat, Deden; Wahyuni, Dewi; Pramartaa, Ikhsan Qodri; Gopar, Ruslan Abdul; Negara, Windu; Nurlaela, Raden Siti
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 3 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i3.767

Abstract

Zeolite had been widely investigated in animal feed for its ability to improve feed efficiency and reduce ammonia emissions, and nano-zeolite offered greater adsorption capacity due to its expanded surface area. This study evaluated the effects of nano-zeolite supplementation on ammonia (NH₃), volatile fatty acids (VFA), the VFA/NH₃ ratio, and pH during in vitro rumen fermentation. A two-factor randomized block design (6 × 6) with four replicates was used. Incubation time (2, 4, 8, 12, 24, and 48 h) served as the first factor, and six dietary treatments served as the second factor: control, urea-supplemented concentrate, inactivated nano-zeolite, activated nano-zeolite, urea-impregnated inactivated nano-zeolite, and urea-impregnated activated nano-zeolite. Nano-zeolite, whether active or urea-impregnated, did not significantly improve rumen fermentation efficiency based on NH₃, VFA, or VFA/NH₃ ratio. All nano-zeolite diets performed similarly to the control and urea-only diets. Fermentation efficiency peaked at 4 and 8 hours of incubation, indicating that nano-zeolite combined with urea supported stable fermentation, particularly during early to mid-incubation.