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All Journal Majalah Kedokteran Bandung Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Berkala Kedokteran Jurnal Berkala Kesehatan Jurnal Sinergitas PkM & CSR Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Ilmiah Berkala: Sains dan Terapan Kimia Homeostasis: Jurnal Mahasiswa Pendidikan Dokter
Alfi Yasmina
Department Of Pharmacology, Faculty Of Medicine, Lambung Mangkurat University, Banjarmasin
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Uji Toksisitas Ekstrak Metanol Kulit Kayu dan Daun Galam dengan Metode BSLT Ahmad Danial Rizkillah Az Zamzami; Isnaini Isnaini; Alfi Yasmina
Homeostasis Vol 4, No 1 (2021)
Publisher : Homeostasis

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Abstract

Abstract: Galam (Malaleuca cajuputi subs. Cuimingiana [Turcz.] Barlow) contains phenolic compounds, flavonoids, alkaloids, tannins, steroids, and terpenoids, which are assumed to have toxic properties. Each part of the plant contains different compounds that might affect its biological activity. This study aimed to analyze the toxicity of the methanol extracts of galam leaves and tree bark with brine shrimp lethality test (BSLT). This research was experimental, and the samples used were the methanol extracts of galam leaves and tree bark with concentrations of 10, 100, 200, 500, and 1000 ppm. Toxicity was assessed with BSLT, and LC50 was analyzed using probit analysis. The results showed that the LC50 value for galam leaves methanol extract was 141.86 (110.77-177.07) ppm and for galam tree bark methanol extract was 610.50 (495.03-745.36) ppm. It can be concluded that galam leaves and tree bark methanol extracts was classified as toxic, with the methanol extract of galam leaves having a higher toxicity than the methanol extract of galam tree bark. Keywords: toxicity, galam, Malaleuca cajuputi, brine shrimp lethality test Abstrak: Tanaman galam (Malaleuca cajuputi subs. Cuimingiana [Turcz.] Barlow) mengandung steroid, fenol, alkaloid, terpenoid, tanin, dan flavonoid yang diduga memiliki sifat toksisitas. Bagian-bagian tanaman memiliki senyawa-senyawa yang berbeda, yang dapat mempengaruhi aktivitas biologisnya. Penelitian ini memiliki tujuan dalam menganalisa toksisitas ekstrak metanol kulit kayu dan daun galam dengan metode brine shrimp lethality test (BSLT). Penelitian ini adalah penelitian eksperimental laboratorik. Sampel yang digunakan adalah ekstrak metanol kulit kayu dan daun galam dengan konsentrasi 10, 100, 200, 500, dan 1000 ppm. Toksisitas dinilai dengan menggunakan metode BSLT dan dinyatakan dengan LC50. LC50 dianalisis menggunakan analisa probit. Hasil penelitian ini menunjukkan nilai LC50 untuk ekstrak metanol daun galam sebesar 141, 86 (110,77-177,07) ppm dan untuk ekstrak metanol kulit kayu galam sebesar 610,50 (495,03-745,36) ppm. Dengan demikian, daun dan kulit kayu galam diklasifikasikan sebagai toksik, dengan ekstrak metanol daun galam mempunyai toksisitas lebih besar daripada ekstrak metanol kulit kayu galam. Kata-kata kunci: toksisitas, galam, Malaleuca cajuputi, brine shrimp lethality test
Perbandingan Aktivitas Antibakteri Ekstrak Daun Tanjung dan Daun Jambu Biji Terhadap Salmonella typhi In Vitro Dini Rizki Aulia; Noor Muthmainah; Alfi Yasmina
Homeostasis Vol 3, No 1 (2020)
Publisher : Homeostasis

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Abstract: Tanjung and guava plant have been known as the famous plant in Indonesia used for antidiarrhea. The active ingredientts of ethanol’ extract of tanjung leaves are alkaloid, tanin and saponin, while the active ingredients of ethanol extract of guava leaves are alkaloid and flavonoid. The purpose of this study was to assess the comparison of antibacterial activity between tanjung leaves and guava leaves ethanol extracts against Salmonella typhi in vitro. The design of the study was true experimental with Posttest-Only with Control Group Design method, using random design group which consisted of the following concentrations of both extract; 6.25%, 12.5%, 25%, 50%, and with chloramphenicol 30 µg as positiv control and aquades as negative conttrol.  The statistical analysis in this study used Shapiro-Wilk, Levene’s Test, Kruskal-Wallis and Mann-Whitney tests. The average of largest inhibitory zone of both extracts with the concentration of 50% was 15.74 mm for tanjung leaves ethanol extract and 14.99 mm for guava leaves ethanol extract. There was significant differences of inhibitory zone diameters between tanjung leaves and guava leaves extract at the concentration of 6.25%, 12.5%, and 50% (p < 0,05). The conclusion of this study showed that ‘the tanjung leaves ethanol extract has significantly higher average of inhibitory zone compared to guava leaves ethanol extract against S. typhi. Keywords: tanjung leaves, guava leaves, Salmonella typhi. Abstrak: Tanaman tanjung dan jambu biji adalah tanaman yang sudah terkenal lama di Indonesia sebagaii antidiare. Kandungan zat aktif yang terdapat pada ekstrak etanol daun tanjung meliputi senyawa alkaloid, tanin, dan saponin,sedangkan ekstrak etanol daun jambu biji memiliki kandungan zat aktif berupa senyawa alkaloid dan flavonoid. Tujuan dari penelitian ini untuk mengetahui perbandingan aktiivitas antibakteri esktrak etanol daun tanjung dan daun jambu biji ‘terhadap Salmonella typhi in vitro. Penelitian ini merupakan penelitian true experimental dengan metode Posttest-Only with Control Group Design, menggunakan rancangan penelitian acak kelompok yang terdiri dari perlakuan konsentrasi ekstrak etanol daun tanjung dan daun jambu biji sebesar 6,25%, 12,5%, 25%, 50% serta kloramfenikol 30 µg sebagai kontrol positif dan aquadest sebagai kontrol negatif. Analisis data penelitian menggunakan uji Shapiro-Wilk, Levene’s Test, Kruskal-Wallis dan Mann-Whitney, dengan tingkat kepercayaan 95%. Rerata zona hambat terbesar yang dihasilkan ekstrak daun tanjung dan daun jambu biji dengan konsentrasi 50% terhadap pertumbuhan S. typhi secara berturut-turut sebesar 15,74 mm dan 14,99 mm. Terdapat perbedaan bermakna diameter zona hambat antara ekstrak daun tanjung dan jambu biji pada konsentrasi 6,25%, 12,5%, dan 50% (p < 0,05). Kesimpulannya adalah ekstrak etanol daun tanjung memiliki rerata zona hambat yang lebih besar secara bermakna dibandingkan ekstrak etanole daun jambu biji terhadap pzertumbuhan S. typhi Kata-kata kunci: daun tanjung, daun jambu biji, Salmonella typhi
Perbandingan Aktivitas Antibakteri Ekstrak Daun Tanjung dan Daun Jambu Biji terhadap Escherichia coli In Vitro Rinny Vebliani; Noor Muthmainah; Alfi Yasmina
Homeostasis Vol 3, No 1 (2020)
Publisher : Homeostasis

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Abstract

Abstract: Tanjung leaves and guava leaves are commonly used by the community as herbal medicines. Guava leaves contain antibacterial compounds, namely, saponins, tannins, alkaloids and flavonoids, while the tanjung leaves contain alkaloids, tannins and saponins. This study aimed to examine the comparison of the antibacterial activity between tanjung leaves extract and guava leaves extract against Escherichia coli in vitro. The methods used in this study was true experimental with post test only with control group design, consisting of 10 treatments of tanjung and guava leaves extracts at the concentrations of 20%, 25%, 30% and 35%, chloramphenicol as positive control, and distilled water as negative control. The parameter measured was the diameter of the inhibitory zone. Data analysis used the Kruskal-Wallis test and the Mann-Whitney test, with 95% confidence level. In this study, the results obtained showed no significant differences between treatments with tanjung leaves extract and guava leaves extracts at the concentrations of 20%, 25%, 30%, and 35% (p > 0.05). The conclusion of this study is that the antibacterial activity of tanjung leaves extract did not differ significantly with guava leaves extract against E. coli at the same concentration. Keywords: Escherichia coli, inhibitoryzone, tanjung leaves, and guava leaves  Abstrak: Daun tanjung dan daun jambu biji biasa digunakan oleh masyarakat sebagai obat herbal. Daun jambu mengandung zat antibakteri yaitu saponin, tanin, alkaloiddanflavonoid, sedangkan pada daun tanjung yaitu alkaloid, tanindansaponin. Penelitian ini bertujuan untuk mengetahui perbandingan aktivitas antibakteri ekstrak daun tanjung dan daun jambu biji terhadap Escherichia coliin vitro. Metode penelitian ini adalah true experimental dengan rancangan post test only with control grup design, terdiri dari 10 perlakuan ekstrak daun tanjung dan daun jambu biji dengan konsentrasi 20%, 25%, 30% dan 35%, kloramfenikol sebagai kontrol positif, serta akuades sebagai kontrol negatif. Parameter yang diukur adalah diameter zona hambat. Analisis data dilakukan dengan menggunakan uji Kruskal-Wallis dan uji Mann-Whitney, dengan tingkat kepercayaan 95%. Pada penelitian ini didapatkan hasil tidak ada perbedaan bermakna antara dari perlakuan ekstrak daun tanjung dan daun jambu biji konsentrasi 20%, 25%, 30%, dan 35% (p > 0,05). Simpulan pada penelitian ini adalah aktivitas antibakteri ekstrak daun tanjung tidakberbeda bermakna secara statistik dibandingkan ekstrak daun jambu biji terhadap E. coli pada konsentrasi yang sama. Kata-kata kunci: Escherichia coli, zona hambat, daun tanjung, daun jambu biji.
Perbandingan Aktivitas Antibakteri antara Ekstrak Daun dan Kulit Batang Tanjung terhadap Staphylococcus aureus In Vitro Nadita Arianti Sumarno; Alfi Yasmina; Noor Muthmainah
Homeostasis Vol 3, No 1 (2020)
Publisher : Homeostasis

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Abstract

Abstract: Tanjung (Mimusops elengi L.) is a medicinal plant that can treat various diseases, one of which is caused by Staphylococcus aureus. This plant contains alkaloid, tannin, saponin, and flavonoid which have effect as antibacterials. The purpose of this study was to determine the comparison of antibacterial activity between tanjung leaves and stem bark extracts against S. aureus in vitro. The method of this study was true experimental with post-test only with control group design, consisting of 10 treatments of tanjung leaves and stem bark extracts with concentrations of 15%, 25%, 35%, and 45%, the positive control (clindamycin 2 μg), and the negative control (aquadest). Extraction method used was maceration with 70% ethanol. The positive control was clindamycin 2 μg, and negative control was aquadest. The measured parameter was the diameter of the inhibitory zone. Statistical analysis used were the Kruskal-Wallis test and the Mann-Whitney test, with 95% confidence level. The results of this study found significant differences between treatments of tanjung leaves and stem bark extracts. Significant differences were found between the treatment with tanjung leaves and stem bark extracts of all concentrations, that is, concentrations of 15%, 25%, 35%, and 45%. The conclusion of this study is that there are significant differences in antibacterial activity between tanjung leaves and stem bark extract in the same concentration against S. aureus in vitro. Keywords: Tanjung leaves, tanjung stem bark, Staphylococcus aureus, inhibitory zone Abstrak: Tanaman tanjung (Mimusops elengi L.) adalah tanaman yang memiliki banyak khasiat dalam mengobati berbagai jenis penyakit, salah satunya penyakit yang disebabkan oleh bakteri Staphylococcus aureus. Tanaman ini memiliki kandungan alkaloid, tanin, saponin, dan flavonoid yang bersifat antibakteri. Tujuan penelitian ini adalah mengetahui perbandingan aktivitas antibakteri antara ekstrak daun dan kulit batang tanjung terhadap S. aureus in vitro. Metode penelitian ini adalah true *(experimental dengan rancangan post test-only with control group design, terdiri dari 8 perlakuan ekstrak daun dan kulit batang tanjung dengan konsentrasi 15%, 25%, 35%, dan 45%, serta kontrol positif klindamisin 2 µg, dan kontrol negatif akuades. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 70%. Kontrol positif klindamisin 2 µg, dan kontrol negatif akuades. Parameter yang diukur adalah diameter zona hambat. Analisis data menggunakan uji Kruskal-Wallis dan uji Mann-Whitney dengan tingkat kepercayaan 95%. Hasil penelitian ini menunjukkan adanya perbedaan bermakna dari perlakuan ekstrak daun dan kulit batang tanjung. Perbedaan yang bermakna terdapat antara perlakuan dengan ekstrak daun dan kulit batang tanjung semua konsentrasi, yaitu konsentrasi 15%, 25%, 35%, dan 45% (p < 0,05). Simpulan pada penelitian ini adalah terdapat perbedaan bermakna aktivitas antibakteri antara ekstrak daun dan kulit batang tanjung pada konsentrasi sama terhadap S. aureus in vitro. Kata-kata kunci: Daun tanjung, kulit batang tanjung, Staphylococcus aureus, Zona hambat
BMI and TLC increase Neoadjuvant Chemotherapy Response in Patients with Locally Advanced Breast Cancer Sasongko Hadi Priyono; Oscar Tri Joko Putra; Alfi Yasmina; Hery Poerwosusanta; Eka Yudha Rahman; Ika Kustiyah Oktaviyanti
Majalah Kedokteran Bandung Vol 54, No 4 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n4.2797

Abstract

Anthracycline-based neoadjuvant chemotherapy is the recommended therapy for locally advanced breast cancer (LABC) patients. Unfortunately, no study has reported the relationship between body mass index (BMI), total lymphocyte count (TLC), and responses to this type of chemotherapy. This study aimed to determine the relationship between BMI, TLC, and response to doxorubicin/epirubicin neoadjuvant chemotherapy in LABC patients. A retrospective cohort design was applied to medical records of LABC patients undergoing neoadjuvant chemotherapy at Ulin General Hospital Banjarmasin, Indonesia, from July to December 2021. BMI and TLC data were assessed based on the values before chemotherapy, while the chemotherapy response was measured using the RECIST 1.1 criteria after 3 cycles. Multinomial logistic regression test with 95% confidence level was used to analyze these data. The results showed that as many as 71% of patients experienced a Partial Response (PR), while 5% and 23% of the patients demonstrated Stable Disease (SD) and Progressive Disease (PD), respectively. Each increase in BMI of 1 kg/m2 was significantly associated with an increase in the occurrence of PR and PD by 1.26 times and 1.29 times, respectively, when compared to the occurrence of PD. Meanwhile, an increase in TLC of 100 cells/mm3 was associated with an increase in the occurrence of PR by 6.83 times and an increase in the occurrence of SD. of 6.94 when compared to the occurrence of PD. Therefore, there is a significant relationship between BMI, TLC, and response to anthracycline-based neoadjuvant chemotherapy in LABC patients
Antioxidant and Antiproliferative Activities of Methanol Extract from Melaleuca cajuputi subsp. Cumingiana [Turcz.] Fruit Isnaini Isnaini; Rosinta Dewi Achmadiyah; Gelvia Awaeh; Husnul Khatimah; Alfi Yasmina
Jurnal Sains dan Terapan Kimia Vol 17, No 1 (2023)
Publisher : Program Studi Kimia, Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jstk.v17i1.13055

Abstract

Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow (M. cajuputi) is a plant that is easily found in Banjarmasin. M. cajuputi contains phytochemical compounds in the form of polyphenols including flavonoids, quinons, saponins, and alkaloids that are thought to have antioxidant and antiproliferation activities. The aim of this research was to find out analyze antioxidant and antiproliferation activity of M. cajuputi fruit methanol extract. The antioxidant activity was tested using the DPPH method. The activities were observed in IC50 and were measured using the UV-VIS spectrophotometer at a wavelength of 517 nm. To test the antiproliferation, true experimental with post-test was applied in this research.  Animal used in this study were 30 mature zebras (length > 2.5 cm) which were grouped into 4, namely the negative control group (DMSO 0.05%), the methanol extract group of M. cajuputi with concentrations of 18.5 ppm, 37 ppm and 74 ppm. The bound variable in this study was antiproliferation activity in the tail of an amputated fish. Data analysis was measured by one-way ANOVA and Post-Hoc Tukey HSD tests. Phytochemical results obtained the presence of phenol compounds, cuinons, flavonoids, alkaloids, saponins, tannins, steroids and terpenoids. Methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit was at IC50 of 15.50 ppm (95% CI 8.31- 32.72).  The antiproliferation activity of zebrafish tails increased in the administration of fruit extract Melaleuca cajuputi subsp. Cumingiana [Turcz.] Barlow in consentration of 74 ppm (p<0.05), both on day 4 and day 8 of measurement when compared to negative controls. It can be concluded that methanol extract of Melaleuca cajuputi subsp Cumingiana [Turcz.] Barlow fruit has antiproliferative activity against the growth of amputated zebrafish tails.
Antibiotics Susceptibility Pattern of MRSA at Intensive Care Room of Ulin General Hospital Banjarmasin Shania Indah Chineko; Dewi Indah Noviana Pratiwi; Rahmiati Rahmiati; Noor Muthmainnah; Alfi Yasmina
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1649

Abstract

Infection caused by Methicillin-Resistant Staphylococcus aureus (MRSA) is a healthcare-associated infection that receives the most significant attention worldwide due to its resistance. Administration of precise and rational antibiotics can prevent high MRSA rates in hospitals. This study aimed to determine the antibiotic susceptibility pattern of MRSA at the intensive care room of Ulin General Hospital, Banjarmasin, between 2016 and 2018. This study was an observational analytic study by taking the results of culture and antibiotic susceptibility pattern data of the MRSA isolated from patients treated at the intensive care room retrospectively. The results showed 37 data of patients suffering from MRSA at the intensive care room in 2016-2018, with a percentage of 23.81%, 25.81%, and 35.19%, respectively. The most common sources of MRSA isolate in this study were sputum (32.39%), blood (29.27%), and pus (16.67%). From 2016 to 2017, there was a decreased susceptibility to macrolide antibiotics, aminoglycosides such as Gentamicin, and quinolones such as Moxifloxacin. In 2018, there was an increased susceptibility pattern of some antibiotics compared to the previous period. Antibiotics with the highest susceptibility in period of 2016-2018 were Linezolid, Quinupristin/Dalfopristin, Tigecycline, Nitrofurantoin, and Trimethoprim/Sulfamethoxazole. Also, the antibiotic with the lowest susceptibility was Tetracycline. It was concluded that there had been changes in some antibiotics' susceptibility pattern to MRSA within 2016-2018.
Antibiotics Susceptibility Pattern in Diabetic Ulcer Patients Mita Rahma Yani; Dewi Indah Noviana Pratiwi; Rahmiati Rahmiati; Noor Muthmainah; Alfi Yasmina
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 27 No. 2 (2021)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v27i2.1652

Abstract

Diabetic ulcers are a chronic complication of diabetes mellitus and have a high risk of infection. Severe ulcer infections are a significant cause of lower-extremity amputations in addition to trauma. Therefore, therapy for diabetic ulcer infections must be performed immediately. This study aimed to determine the bacterial susceptibility pattern to the antibiotic in diabetic ulcer patients. This study was retrospective observational descriptive by taking the results of swab culture and antibiotic susceptibility patterns data in diabetic ulcer patients at Ulin General Hospital, Banjarmasin, in 2016-2018. The results showed 41 (62.1%) monomicrobial infections and 25 (37.9%) polymicrobial infections. The number of Gram-negative bacilli (57.4%) was higher than Gram-positive cocci (42.6%). The most common bacterial isolates on pus culture were Staphylococcus aureus (26.6%), Klebsiella pneumonia (19.1%), and Escherichia coli (12.8%). Antibiotic susceptibility test results showed that Gram-positive bacteria were sensitive to Tigecycline (100%), Nitrofurantoin (96.9%), and Linezolid (96.8%). Gram-negative bacteria were susceptible to Ertapenem (92.7%), Meropenem, and Amikacin (90.6%). S.aureus isolates were sensitive 100% to Meropenem and Tigecycline. K.peneumoniae and E.coli isolates were susceptible 100% to Meropenem and Amikacin. It was concluded in this study that the prevalence of Gram-negative bacteria in diabetic ulcer infection was higher than Gram-positive bacteria. The most common isolated Gram-negative bacteria were K.pneumoniae and E.coli, while the most common Gram-positive bacteria were S.aureus. The most sensitive antibiotics for K.pneumoniae and E.coli were Meropenem and Amikacin, while the most sensitive antibiotics for S.aureus were Linezolid and Tigecycline.
Co-Authors Agung Biworo Ahmad Danial Rizkillah Az Zamzami Ahmad Zaki Hafizi Anwari Delmi Arifin Syamsul Dewi Indah Noviana Pratiwi Dini Rizki Aulia Dona Marisa, Dona Dwi Indria Anggraini Dwi Laksono Adiputro Een Amalia Pratiwi Eka Yudha Rahman Erna Kristin Gelvia Awaeh Harapan Parlindungan Ringoringo Hery Poerwosusanta I Nyoman Suarjana Ika Kustiyah Oktaviyanti Irhamna Syari Yani Yani Isnaini Isnaini Jarir At Thobari Kharis Faridan Khatimah, Husnul Larasisca Della Faradilla Lia Yulia Budiarti Meitria Syahadatina Noor Mita Rahma Yani Muthmainah, Noor Muthmainnah, Noor Nadita Arianti Sumarno Nurul Hidayah Oscar Tri Joko Putra Rahmiati Rahmiati Rahmiati Rahmiati Rinny Vebliani Rosinta Dewi Achmadiyah Sasongko Hadi Priyono Shania Indah Chineko Siti Kaidah