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Sukarjati
Universitas PGRI Adi Buana Surabaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Potensi Ekstrak Daun Sambung Nyawa (Gynura procumens) Dan Biji Mahoni (Swietenia mahagoni jacq) Serta Kombinasi Kedua Ekstrak Terhadap Berat Testis Mencit (Mus musculus) Hiperglikimia Sanima Laia; Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 12 No 02 (2019)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.12.02.2306.89-97

Abstract

Diabetes melitus (DM) adalah suatu penyakit metabolik yang ditandai dengan hiperglikemia.terhadap sel leydig, diameter tubulus seminiferus dan berat testis. daun sambung nyawa (Gynura procumens) dan biji mahoni (Switenia mahagoni jaqc). banyak ditanam sebagai tanaman hias, semak, dan tumbuh di daerah tropis dan subtropis. Daun sambung nyawa (Gynura procumens) dan biji mahoni (Switenia mahagoni jaqc) juga merupakan salah satu jenis tanaman yang berpotensi sebagai antifertilitas. Jenis senyawa bioaktif yang terkandung pada tumbuhan, utamanya senyawa-senyawa yang berasal dari golongan steroid, alkaloid, isoflavanoid, tripernoid, dan xanthon memiliki aktivitas sebagai bahan antifertilitas. Tujuan penelitian ini adalah mengetahui pemberian ekstrak Daun sambung nyawa (Gynura procumens) dan biji mahoni (Switenia mahagoni jaqc) serta kombinasinya kedua ekstrak jumlah sel leydig, diameter tubulus seminiferus dan berat testis. Sampel penelitian ini adalah mencit sebanyak 48 ekor dengan berat badan 25-30 gram, berumur 2-2,5 bulan. Mencit di bagi 12 kelompok, masing-masing kelompok dibagi 4 perlakuan. Metode pembuatan ekstrak dangan menggunakan maserasi Adapun perlakuan yang diberikan adalah ekstrak Daun sambung nyawa (Gynura procumens) serta kombinasi kedua ekstrak dengan dosis kontrol, 200 mg/kg BB, 250 mg/kg BB dan 300 mg/kg BB dan biji mahoni (Switenia mahagoni jaqc) dengan dosis kontrol, 200 mg/kg BB, 250 mg/kg BB dan 300 mg/kg BB serta kombinasi kedua ektrak dengan dosis 100:100 mg/kg BB, 125:125 mg/kg BB dan 150:150 mg/kg BB pemberian ekstrak selama 35 hari. Pada hari ke 37 mencit di bedah untuk diambil testis untuk pengamatan penimbangan berat testis.Testis kemudian dibuat preparat histologi dan di hitung sel leydig, diukur diameter tubulus seminiferus. berat testis di timbang dengan menggunakan alat timbang analitik yang memiliki akurasi o,o1 gram, sel leydig diamati di dalam preparat dengan alat mikroskop, diamter tubulus seminiferus dihitung dengan alat mikrometer yang di letakkan di dalam tabung lensa objektif miroskop pembesaran 400x10 merupakan penelitian eksperimental dengan menggunakan Rancangan acak lengkap (RAL). Data yang diperoleh dianalisis dengan analisis uji F, varian (ANOVA) Satu arah dan dilanjutkan dengan uji LSD (Least Significant Different). Hasil dari penelitian ini menujukkan ada pengaruh ekstrak daun sambung nyawa (Gynura procumens) dan biji mahoni (Switenia mahagoni jaqc) serta kombinsa kedua ekstrak terhadap jumlah sel leydig, diameter tubulus dan berat testis, (P<0,05), Perlakuan terbaik yang dapat meningkatkan sel leydig, diameter tubulus seminiferus dan berat testis berat, adalah kombinsa kedua ekstrak dengan dosis 150:150 mg/kg BB. Hasil penelitian ini dapat disimpulkan bahwa ekstrak daun sambung nyawa (Gynura procumens) dan biji mahoni (Switenia mahagoni jaqc) serta kombinsa kedua ekstrak dapat meningkatkan jumlah sel leydig, diameter tubulus seminiferus dan berat testis. Kedepannya diharapkan penelitian dapat dikembangkan sebagai bahan antifertilitas pada pria. Kata Kunci: ekstrak daun sambung nyawa, dan biji mahoni, berat testis mencit hiperglikemia.
DETEKSI DELESI GEN DAZ (Deleted in AZoospermia) PADA PRIA AZOOSPERMIA DENGAN METODE PCR (Polymerase Chain Reaction) V.A. Ferandra; Sukarjati Sukarjati
TEKNOLOGI MEDIS DAN JURNAL KESEHATAN UMUM Vol 1 No 1 (2017): Medical Technology and Public Health Journal March 2017
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mtphj.v1i1.759

Abstract

At this time the case of azoospermia is quite common in infertile men. Azoospermia is a condition where the semen does not contain sperm. Many causes azoospermia, including the deletion of a gene at the locus that is located on the Y chromosome long arm (YQ) known as AZF gene (Azoospermia Factor). One of the genes in the AZF region are genes that AZFc DAZ (Deleted in Azoospermia). The purpose of this study was to detect the presence of the DAZ gene deletions in men with azoospermia cases using PCR (Polymerase Chain Reaction). The study design was descriptive. Venous blood samples with EDTA anticoagulant taken from 10 men azoospeermia then extracted to obtain DNA. DNA samples were then carried out PCR with primers DAZ. The PCR products were separated by electrophoresis in 2% agarose gel and visualized using UVtranslluminator. Of the 10 samples, four patients including DAZ gene deletion was detected experience while the other six do not experience DAZ gene deletions. It concluded that found their DAZ gene deletions in men with azoospermia using the PCR method
KERUPUK SAMILER FORTIFIKASI KALSIUM DARI DURI IKAN BANDENG Sukarjati Sukarjati; Sulistyowati Sulistyowati
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 6, No 2 (2015): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v6i2.952

Abstract

Kerupuk Samiler cukup dihargai oleh nilai gizi masyarakat rendah karena hanya terbuat dari singkong. Proses produksi tidak higienis karena dicetak menggunakan bak plastik warna warni. Pengeringan hanya mengandalkan sinar matahari. Hal ini menyebabkan tidak ada penghasilan tetap. Pemasaran terbatas pada daerah sekitar produksi dan disimpan di toko atau dijual keliling. Tujuan dari program IbM ini adalah untuk mengatasi masalah tersebut. Metode yang digunakan adalah konseling, pelatihan, dan mentoring dalam produksi kerupuk samiler asli dan kerupuk samiler fortifikasi duri ikan yang higienis dan melakukan pelatihan dan mentoring manajemen produksi dan manajemen pemasaran. Hasil dari program ini adalah berdasarkan uji kadar kalsium dalam adonan kerupuk samiler yang diberi duri ikan setinggi 51.28 mg/100g dibandingkan dengan adonan kerupuk yang tidak diberi duri ikan yaitu 9.88 mg/100 g. Keterampilan dan pengetahuan pembuat kerupuk samiler tentang manajemen produksi dan manajemen pemasaran meningkat. Telah diproduksi kerupuk samiler asli dan fortifikasi kalsium duri bandeng. Kedua produk ini diproses dan dikemas secara higienis. Pengeringan kerupuk di musim hujan dapat diatasi menggunakan mesin pengering kerupuk dengan bahan bakar LPG. Kesimpulan dari program IbM adalah pembuat kerupuk samiler meningkatkan pendapatan dan penghasilan tetap. ?é?á Kata Kunci: Kerupuk Samiler, Fortifikasi Kalsium, Duri Bandeng
PENGARUH ESCHERICHIA COLI DAN GRANULOSIT TERHADAP KADAR REACTIVE OXYGEN SPECIES SECARA IN VITRO Sukarjati; Doddy M. Soebady; Aucky Hinting; Sudjarwo
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 15 No 2 (2010): June 2010
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/268

Abstract

In humans, male genital tract infection has been recognized as one of the causes of infertility. Indicators of the occurrence of genital tract infection are the presence of bacteria during semen culture (bacteriospermia) and the finding of leucocyte of more than 1 million/ml semen (leucotytospermia). Escherichia coli (E. coli) is the most common cause of prostatitis and epididymitis. The objective of this study was to determine the effects of E. coli and granulocytes on Reactive Oxygen Species (ROS) level in vitro. This study comprised of two experiments. In the experiment 1, sperm was incubated with E. coli, and the experiment 2, the sperm was incubated with granulocyte. In those experiments, ROS levels were observed. Spermatozoa were obtained from donor with normal spermatozoa according to WHO (1999). Escherichia coli was obtained by culturing the semen of infertile males. Granulocytes were obtained from donors’ blood. Sperm preparation was made by using Percoll gradient column method. Granulocyte isolation used Histopaque 1077 and 1119. ROS level was detected by means of chemiluminescence method with beta counter device. The result of this study showed that in vitro E. coli had the effect on ROS level, both stimulated by peroxidase (p = 0.000) and PMA (p = 0.006). Granulocyte had effect on ROS level. In peroxidase-stimulated ROS level, there was the effect between spermatozoa and granulocyte-incubated sperm (p = 0.000), granulocyte-incubated sperm and granulocyte (p = 0.002), and sperm and granulocyte (p = 0.000). In PMA-stimulated ROS level, there was effect between sperm and granulocyte-incubated sperm (p = 0.000), sperm and granulocyte (p = 0.000), granulocyte-incubated sperm and granulocyte (p = 0.000). In conclusion, under in vitro experiment, sperm incubated with E. coli and sperm incubated with granulocyte had the effect on the level of the ROS.
PENGARUH SPESIES BAKTERI DAN RATIO SPERMATOZOA/BAKTERI TERHADAP VITALITAS SPERMATOZOA MANUSIA SECARA IN VITRO Sukarjati Sukarjati; Hamdani Lunardhi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 7 No 1 (2001): December 2001
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/508

Abstract

The effect of some pecies of bacteria and sperm/bacteria ratio on sperm vitality has been studied. Four species of bacteria were used in this study: Stapylococcus epidermidis, Streptococcus faecalis, Enterobacter aerogenes were obtained from semen culture of infertile men and E. coli was obtained from prostaltic fluid culture from men with prostat and urinary system disturbances. Five semen samples fulfilling the WHO criteri (1992) were used in this study. After preparation by Percoll gradient-column method, sperm were inoculated in a microplate with Stapylococcus epidermidis, Streptococcus faecalis, Enterobacter aerogenes and E. coli under the sperm/bacteria ratio 1:10 and 1:1000. Sperm vitality was observed immediately, 3 and 6 hours after inoculation. At the second experiment, the detrimental influence of bacteria on sperm was prevented by adding penicillin. Result of this study indicated that Stapylococcus epidermidis, Streptococcus faecalis, and Enterobacter aerogenes were not affected on the sperm vitality. The effect of E. coli on sperm vitality occurred at the ratio of sperm/bacteria 1:10 after 3 and 6 hours incubation and at the ratio of sperm/bacteria 1:1000 occurred after 6 hours incubation. It might be concluded that the negative influence of bacteria on sperm vitality in vitro, is dependent in species of bacteria, bacteria concentration, and time of incubation. The most detrimental effect on sperm vitality was shown by E. coli at the ratio of sperm/bacteria 1:10 after 6 hours incubation. The detrimental effecr was not prevented bt the addition of penicillin.
PENGARUH EKSTRAK ETANOL BUNGA, DAUN, DAN AKAR KEMBANG SEPATU (Hibiscus rosa sinensis) TERHADAP BERAT, VOLUME, DAN DIAMETER TUBULUS SEMINIFERUS MENCIT (Mus musculus) Rojib Rojib; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 9 No 01 (2016)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol9.no01.a329

Abstract

Hibiscus (Hibiscus rosa sinensis) many are grown as ornamental plants, shrubs, and grown in the tropics andsubtropics. Hibiscus is also one kind of plant that is potentially as antifertility. Types of bioactif compoundscontained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid,tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence ofethanol ekstract flowers, leaves, and roots of hibiscus (Hibiscus rosa sinensis) against the weight, volumeand diameter ofseminiferoustubules mice (Mus musculus). The sample of this research is the mice by asmuch as 27 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided3 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dosecontrol, 150, and 300 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicularweight to observations taken, the volume of the testes. The testes then made preparations and measureddiameter of seminiferoustubules. This research is experimental research using Random Design Group. Thedata obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD(Least Significant Different). The results of the research there shows the influence of the ethanol extracts offlowers, leaves, and roots of hibiscus against testicular volume, weight, and diameter of seminferoustubules (P < 0.05). The best treatment that can decrease the weight, volume and diameterofseminiferoustubules is on the flower with a dose of 300 mg/kg. The results of this research it can beconcluded that extracts of flowers, leaves, and roots can lose weight, testicular volume and diameter ofseminiferoustubules. In future it is hoped the research could be developed as material antifertilitas in men. Keywords:Extract of flower, Laves, roots of (Hibiscus rosa sinensis), testicles, diameter of seminiferous tubules, mice (Mus musculus).
PENGARUH EKSTRAK ETIL ASETAT GETAH KAMBOJA (Plumeria Acumenate.W.T.Ait) TERHADAP PERTUMBUHAN DAN DAYA HAMBAT BAKTERI Staphylococus aureus R. T. Wahyudi; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 6 No 02 (2013)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol6.no02.a525

Abstract

Frangipani sap (Plumeria acumenate.WTAit) was known to use as an antiseptic. This study aimed to prove ethyl acetate extract of frangipani sap as antibacterial Staphylococcus aureus. Frangipani sap from apical stem was extracted with chloroform and fractionated with ethyl acetate. Concentration of ethyl acetate extract (0%, 5%, 10%, 15%, 20%, and 25%) were tested against the growth of bacteria S. aureus using plate count and diffusion agar method. The results showed concentrations of frangipani sap extract significantly (P <0.05) on the growth of S. aureus. Highest inhibitions of frangipani sap extract on growth of S. aureus have obtained at a concentration of 25%. Key words: frangipani sap, ethyl acetate, S. aureus
PENGARUH EKSTRAK BIJI PEPAYA (carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) DAN CAMPURAN EKSTRAK BIJI PEPAYA ( carica papaya L.) DAN EKSTRAK DAUN MIMBA (Azadirachta indica A.Juss) TERHADAP DIAMETER TUBULUS SEMINIFERUS, SEL LEYDIG DAN BOBOT TESTIS MENCIT (Mus musculus) Apolonia M; Sukarjati sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 01 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no1.a634

Abstract

Papaya (Carica papaya L. ) and Neem (Azadirachia indica A.Juss) Tree are planted as an ornamental tree and have a character as a brush plant. They vegetated in tropical and subtropical region. The seed of papaya and Neem leaf are also include of a potential plants as a antifertility. Types of bioactif compounds contained in plants, the main compounds that are derived from the steroids, alkaloids, isoflavanoid, tripernoid, and activity as xanthon antifertilities. This encourages the researcher to know the influence of ethanol seed papaya extract and neem leaf extract towards diameter of seminiferous tubule, leydig cell and the testicular weight mice (Mus musculus). The sample of this research is the mice by as much as 21 tail with a weight of 20-30 grams, aged 2.5 months. Murine in to 3 groups, each group is divided 7 treatment. As for the treatments given are extracts of flowers, leaves, and roots of hibiscus with dose control, 50, and 100 mg/kg. Granting of extracts for 35 days. On day 37 mice are in surgery for testicular weight to observations taken, the volume of the testes. The testes then made preparations and measured diameter of seminiferous tubules. This research is experimental research using Random Complete Group. The data obtained were analyzed with variant analysis (ANOVA) in one direction and continued with test LSD (Least Significant Different). The results of the research there shows the influence of the ethanol papaya seed extract, meem leaf extracy and also the micture of papaya seed extract and neem leaf extract towards the weight of mice, leydig cell and the diameter of seminiferous tubule (P < 0.05). The best result to decrease the testicular weight and diameter of seminiferous tubule was showed by the neem with the weight of 100 mg/kg BB. The best result to decrease the leydig cell was showed on the mixture of papaya seed extract and neem leaf extract in dosage of 100 mg/kg BB. This study showed that papaya seed extract, neem leaf extract and the mixture of papaya seed extract and neem leaf extract able to decrease the testicular weight, diameter of seminiferous and leydig cell. In the next day, this research supposed to be able as an antifertility in male.
KONSENTRASI HAMBAT MINIMUM EKSTRAK ETHANOL DAUN BELUNTAS (Pluchea indicha) DAN DAUN KEMANGI (Ocimum sanctum L) SERTA CAMPURAN DAUN BELUNTAS DAN DAUN KEMANGI TERHADAP PERTUMBUHAN BAKTERI (Escherichia coli, Staphylococcus aureus) PADA PENDERITA PROSTATITIS Isnainy Hidayati; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 10 No 02 (2017)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol10.no2.a1030

Abstract

Prostatitis is a disease caused by bacteria, especially (E. coli, S. aureus). Treatment of diseases using antibiotics cause negative effects on the environment, pathogenic bacterial resistance, and antibiotic residues. Therefore, it is necessary to develop an antibacterial alternative from natural ingredients for the treatment of this disease. One of the natural ingredients that are antibacterial is basil leaf extract and beluntas leaves that have antibacterial inhibitory power. This research aims to prove the antibacterial activity of basil leaf extract and beluntas leaves in vitro. The treatments used were P0 (control), P1 (1.56%), P2 (3.125%), P3 (6.25%), P4 (12.5%), P5 (25%), P6 (50% ), P7 (100% ).. The parameters observed were bacterial growth on culture medium. The result of MIC (the lowest concentration of dilution that can inhibit bacterial growth) is seen from the treatment media turbidity and MBC result (the lowest concentration of dilution that can kill bacteria) seen from bacterial colony growth on NAP media. Data were analyzed descriptively. The results showed that basil leaf extract and beluntas leaves can inhibit growth or killing (E. coli, S. aureus) in prostatitis patients. Keywords: Prostatitis, antibacterial activity, microbial inhibition
PENGARUH PEMBERIAN EKSTRAK ETANOL BUNGA, DAUN DAN AKAR KEMBANG SEPATU (Hibiscus rosa sinensis) TERHADAP HISTOLOGI TESTIS MENCIT (Muss muscullus) D. Febrianti; Sukarjati Sukarjati
STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Vol 8 No 02 (2015)
Publisher : FMIPA : Universitas PGRI Adi Buana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/stigma.vol8.no02.a1581

Abstract

Kembang sepatu ( Hibiscus rosa sinensis) merupakan salah satu tanaman herbal. Pada bagian bunga memiliki kandungan flavonoid, pada bagian daun mengandung saponin dan flavonoid sedangkan pada bagian akar mengandung flavonoid, saponin dan tanin. Senyawa flavonoid, saponin, dan tanin ini merupakan senyawa yang berfungsi sebagai antifertilitas untuk kaum pria. Hal inilah yang mendorong penulis untuk melakukan penelitian mengenai pengaruh ekstrak bunga, daun, dan akar kembang sepatu (Hibiscus rosa sinensis) terhadap histologi testis mencit ( Mus muscullus). Sampel penelitian ini adalah testis mencit sebanyak 27 ekor dengan berat badan 20-30 gram, berumur 2,5 bulan. Mencit di bagi 3 kelompok, masing-masing kelompok dibagi 3 perlakuan. Adapun perlakuan yang diberikan adalah ekstrak bunga, ekstrak daun, dan ekstrak akar kembang sepatu dengan konsentrasi 0mg/kg bb, 150 mg/kg bb dan 300 mg/kg bb. Pemberian ekstrak dilakukan 35 hari lamanya. Pada hari ke 37 mencit di bedah untuk diambil cauda testisnya untuk diamati histologi testis pada mencit. Penelitian ini merupakan penelitian eksperimental dengan menggunakan Rancangan acak kelompok (RAK). Pengamatan histologi testis mencit meliputi jumlah sel spermatogonia, sel spermatosit, sel spermatid, dan sel leydig dengan menggunakan mikroskop. Data yang diperoleh dianalisis dengan analisis varian (ANOVA) Satu arah. Hasil dari penelitian ini menujukkan ada pengaruh ekstrak bunga, daun, dan akar kembang sepatu terhadap jumlah sel spermatogonia (P<0,05), jumlah sel spermatosit (P<0,05), jumlah sel spermatid (P<0,05) dan jumlah sel leydig (P<0,05). Hasil penelitian ini dapat disimpulkan bahwa ekstrak daun dengan konsentrasi 300 mg/kg bb adalah perlakuan yang optimal dalam menurunkan jumlah sel spermatogonia, jumlah sel spermatosit, jumlah sel spermatid dan jumlah sel leydig.
Co-Authors ., Intashofal Ainuris Zakiyah Aldi Syahputra Alfian Dwi Cipta Yani Apolonia M Arif Yachya Asti Rahayu Astuti Dewi Aucky Hinting Aulani F. Fithroh Aulani F. Fithroh D. Febrianti DesyaTresty Medistriani Dewanti, Felicitas Deru Dewi, Astuti Diah Karunia Binawati Diaz P Rachmatin Doddy M. Soebady Dyah Hariani Eka p F Erni Widyaswati Ersanto ersanto ersanto, Ersanto Ervina Ervina Evarina, Melinda Yuti F, Eka p Febrianti Febrianti Febrianti Febrianti Febrianti, D. Febrianti, D. Feri Wiranata Ganda Adi Nugroho Gusti Azzahra Widiaura Hamdani Lunardhi Hasanah, Waqiatul Hidayati, Isnainy Hidayati, Isnainy I.A.K. Pramushinta IAK Pramushinta Isnainy Hidayati Laia, Sanima M, Apolonia M, Apolonia M. Saftian Andreanto Masruroh Masruroh Masruroh MASRUROH Melinda Yuti Evarina Mitha Novia Sari Nanis Choirunnisa Ngadiani Nora Augustien Nova Triani Novi Dwi Kusuma Nugroho , Ganda Adi Nur Asiah Nur Syamsiatul Fajar Nurul Islam Fi Holki Pratama, Yoga Bagas Pungky Slamet Wisnu Kusuma Pungky Slamet WK Pungky Slamet WK Pungky SWK Purity Sabila Ajiningrum R. T. Wahyudi Rachmatin, Diaz P Rahayu, Asti Rahayu Rojib Rojib Rojib, Rojib Rojib, Rojib Sanima Laia Sudjarwo Sudjarwo Sudjarwo Sulistyowati Sulistyowati Susie Amilah Tatang Sopandi Tony Susilo Wibowo Umi Nurhayati Umi Nurhayati V.A. Ferandra Vivin Andriani Wahyudi, R. T. Wahyudi, R. T. Waqiatul Hasanah WK, Pungky Slamet Yoga Bagas Pratama