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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abadimas Adi Buana Jurnal Penamas Adi Buana Wahana : Tridarma Perguruan Tinggi Medical and Health Science Journal Bioscientist : Jurnal Ilmiah Biologi Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Lux Mensana: Journal of Scientific Health Folia Medica Indonesiana
Sukarjati Sukarjati, Sukarjati
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Enhancing Spermatozoa Quality and Spermatogenic Cell in Hyperglycemic Mice (Mus musculus L.) with Sambung Nyawa (Gynura procumbens) and Mahagany (Swietenia mahagoni jacq) extracts, Alone and in Combination Sukarjati; Nurhayati, Umi; Medistriani, DesyaTresty
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7180

Abstract

This study aimed to investigate the effect of the ethanol extract of sambung nyawa (Gynura procumbens) leaves, mahogany (Swietenia mahagoni jacq) seeds, and their combination on the motility, viability, morphology, and concentration of spermatozoa, spermatogonia, spermatocytes, and spermatids in hyperglycemic mice induced with 10% dextrose. A total of 48 male mice were used as experimental animals, and the extracts were administered orally for 35 days. On day 36, spermatozoa and testicular tissues were collected for analysis. The results showed that the extracts at various doses had the potential to reduce blood glucose levels and improve the motility, viability, morphology, and concentration of spermatozoa and germ cells in hyperglycemic mice. Result:The combination of sambung nyawa leaves and mahogany seeds at a dose of 150:150 mg/kg BW was found to be the most effective in reducing blood sugar levels and improving sperm parameters. Therefore, these extracts have the potential to be used as alternative herbal ingredients with similar effects to metformin in reducing diabetes and improving fertility.
The Effect of Potassium Dosage and Branch-Pruning on the Growth and Yield of Butternut Squash (Cucurbita moschata Durch) Dewanti, F. Deru; Augustien, Nora; Triani, Nova; Holki, Nurul Islam Fi; Sukarjati
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.7565

Abstract

Butternut squash (C. moschata) is a honey squash plant (Cucurbita moschata Durch) which is an introduced plant from the lowlands of South America and has adapted to Indonesia. The research was carried out in the planting area of Kampung Labu Madu, Toyoresmi Village, Ngasem District, Kediri Regency from January to June 2021. Potassium is among major nutrients needed by plants in their growth and development.  Potassium fertilization can help plants improve the regulation of photosynthetic mechanisms, carbohydrate translocation, protein synthesis This research was a two-factor factorial experiment prepared using a Split Plot Design. The first factor (main plot) was the branch pruning (P) treatment which consisted of 2 levels and the second factor (subplot) was the dose of KCl (K) fertilizer which consisted of 4 levels. The results of the research showed that there was a real influence in the treatment without pruning on the parameters of the number of male flowers and on the parameter of fruit weight with the best treatment being pruning, whereas for the treatment the dose of KCl fertilizer had a real effect on the parameters.
The Impact of Smoking on Sperm Quality and the DAZ Gene Sukarjati; Choirunnisa, Nanis; Fajar, Nur Syamsiatul
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.9213

Abstract

Indonesia's male population is mostly smokers. The chemical content in cigarettes can reduce the quality of spermatogenesis, where spermatogenesis is influenced by the DAZ gene (deleted in Azoospermia). This study aims to analyze sperm quality in light, moderate, and heavy smokers in 3 age groups and analyze the presence of the DAZ gene in heavy smokers. This study used 180 samples divided into three age groups, namely men aged 26-30 years, 31-35 years, and 36-40 years. Each age group recorded the number of cigarettes consumed per day (light, moderate, and heavy smokers) and examined the quality of sperm. While the DAZ gene analysis used 10 samples of heavy male smokers, aged 25-40 years. Sperm quality analysis was based on the WHO laboratory manual for human semen testing, while DAZ gene analysis used the PCR method. Sperm quality data were analyzed using Kruskal-Wallis, while the DAZ gene was analyzed qualitatively descriptively by analyzing electrophoresis photos. The results of this study can be concluded that daily cigarette consumption in the age group affects sperm quality and in heavy smokers, there are 10% who experience deletions in the DAZ gene. Thus cigarettes can reduce spermatozoa quality.
Leaf and Petiole Extract of Centella Asiatica are Potential for Antifertility and Antimicrobial Material Amilah, Susie; Sukarjati, Sukarjati; Rachmatin, Diaz P; Masruroh, Masruroh
Folia Medica Indonesiana Vol. 55, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Ethanol extract of leaves and petiole of pegagan (Centella asiatica) is potential as antimicrobial and antifertility. This study was to investigate whether ethanol extracts of leaf and petiole of Centella asiatica has potential as an antimicrobial and can decrease sperm quality and spermatogenic cells of mice. This was a randomized controlled study using Centella asiatica plant from which we made ethanol extract. The extract of the leaves and petiole of Centella asiatica was subjected to antimicrobial test using the bacteria S. aureus, S. epidermidis, and A. aerogenes. Then the observation was done after a 24-hour-old bacterial cultures by measuring the diameter of the clear zone in mm. Thereafter, the extract was given to randomly grouped mice that were divided into two groups receiving the leaf extract in one group and the petiole exract in another for 35 days. On day 36 the mice were sacrificed for spermatozoa quality and testicular histology examination. Statistical analyses used in this study were ANOVA and LSD tests. There was inhibition zone difference between leaf and petiole (p=0.000). Inhibition zone between bacteria S. epidermidis, S. aureus and A. aerogenes showed no difference (p=0.198). Inter-dose inhibition zone showed difference (p=0.000). Centella asiatica leaf extract showed inhibition zone better than that of Centella asiatica petiole extract. In spermatozoa quality test, there was effect of Centella asiatica leaf extract on sperm motility (p<0.05), viability (p<0.05), morphology (p<0.05) and concentration (p<0.05). No effect of pegagan petiole extract was found on sperm motility (p<0.05), viability (p<0.05), abnormal morphology (p<0.05) and concentration (p<0.05). Highest degradation of spermatozoa quality was obtained from Centella asiatica petiole extract at a concentration of 150 mg/kg bw. Spermatogenic cell test results showed no effect of Centella asiatica leaf extract in decreasing spermatogonia (p=0.000), spermatocytes (p=0.000), and spermatids (p=0.000) counts. No effect of pegagan petiole extract in decreasing the number of spermatogonia, spermatocytes and spermatids. The highest reduction spermatogenic cells of seminiferous tubules was obtained by Centella asiatica petiole extract in a concentration of 150 mg/kg bw. In conclusion: Centella asiatica leaf and petiole extract has antimicrobial potency as contra-ceptives
Role of immunoglobulin g (igg) from the induction of escherichia coli pili adhesion protein isolated from infertile male semen with 32.2 kda molecular weight as opsonin and anti-adhesion an in vitro escherichia coli infection Sukarjati, Sukarjati; Amilah, Susie
Folia Medica Indonesiana Vol. 53, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli), a major cause of male genital tract infections, is asymptomatic and may result in male infertility. We have succeeded in isolating and characterizing proteins of E. Pili coli isolates from semen of infertile men who function as adhesin with a molecular weight (MW) 32.2 kDa. This study aims to prove the ability of IgG results adhesion proteins induced pili of E. MW coli 32.2 kDa as opsonin to determine the value of the activity and phagocytic capacity and as an anti- adhesion by calculating the average number of E. coli that attached to human spermatozoa. E. coli infertile men's semen were cultured using standard bacteriology. Peritoneal macrophages were isolated from mice. Spermatozoa from donors were prepared using Sil with Select Plus. IgG was obtained from mice immunized with (1) PBS (control), (2) E. coli pili adhesion protein isolated from infertile men semen with MW of 32.2 kDa and (3) weakened E. coli isolated from infertile men's semen. Phagocytic activity value was determined by counting the number of cells activated macrophage phagocytosis process in 100 cells. Phagocytic capacity value was determined by counting the number of bacteria ingested by 25 macrophages. Anti-adhesion test was done by counting the number of bacteria attached to 100 spermatozoa. The results of this study showed difference (p=0.000) in phagocytic activity and phagocytic capacity (p=0.000) between treatment (1) and (2), and between treatment (1) to (3). However, treatment (2) and (3) did not differ neither in phagocytic activity (p=0.693) nor in phagocytosis capacity (p=0.125). Anti-adhesion test produces difference (p=0.000) in the number of E. coli that attached to human spermatozoa between treatments (1) and treatment (2), and between treatments (1) and (3). The number of E. coli that attached to human spermatozoa between treatment (2) and treatment (3) was not significantly different (p=0.371). In conclusion, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa can increase phagocytic activity and capacity as well as serve as an anti- adhesion. Thus, IgG from the induction of E. coli pili adhesion protein of infertile men semen isolates with MW of 32.2 kDa is protective against in vitro E. coli infection, so that it can be used as material to prevent male reproductive tract infections due to E. coli.
Toxicity of 32.2 kDa MW Escherichia coli Pili Adhesin Isolated from Infertile Male Semen in Reproductive System Sukarjati, Sukarjati; Amilah, Susie; Sudjarwo, Sudjarwo
Folia Medica Indonesiana Vol. 54, No. 2
Publisher : Folia Medica Indonesiana

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Abstract

Escherichia coli (E. coli) is the leading cause of male genital tract infection with no symptoms of infertility. Protein E. coli pili hemagglutinin isolated from infertile male sperm with 32.2 kDa MW acts as adhesion in spermatozoa. This study aimed to prove whether E. coli pili adhesin 32.2 kDa MW is toxic to male reproductive system. Samples consisted of spermatozoa of 30 guinea pigs divided into three groups: control, immunized with E. coli pili adhesin 32.2 kDa MW protein, and transurethral infected E. coli. Observations of sperm motility, vitality and morphology were performed under a microscope. MDA levels and sperm DNA damage were measured by a spectrophotometer and comet assay method and observed using a fluorescent microscope. There was no difference between control and immunization group of E. coli pili adhesin in motility (p=0.499), vitality (p=0.817) and morphology (p=0.176); between control and transuretral infection groups in motility (p=0.000), vitality (p=0.000) and morphology (p=0.000); and between control and both treatment groups in motility (p=0.001), vitality (p=0,000) and morphology (p=0.000). Histologic analysis showed E. coli pili adhesin of 32.2 kDa MW immunization group did not suffer from testicular tissue damage, while the positive group showed a deterioration of seminiferous tubular cells. MDA levels differed between immunization group E. coli pili, transurethral infection group, and control (p=0.024) and between transurethral and control (p=0.007) groups. However, between control and immunized group with E. coli pili protein showed no difference (p=0.251). DNA damage differed (p=0.000) between immunized group with E. coli pili, transurethral infection and control group; between control and transurethral infected group (p=0.000); and between transurethral infection group and E. coli pili protein immunization group (p=0.000). However, between control and E. coli pili immunization group showed no difference (p=0.600). In conclusion, E. coli pili adhesin 32.2 kDa MW protein is not toxic for sperm quality and the quality of sperm molecules.
PEMBERDAYAAN MASYARAKAT MELALUI INOVASI PRODUK MIE DAUN KELOR SEBAGAI PANGAN FUNGSIONAL BERGIZI TINGGI Ajiningrum, Purity Sabila; Arif Yachya; Tatang Sopandi; Sukarjati; Vivin Andriani; Pungky Slamet Wisnu Kusuma; Diah Karunia Binawati
Jurnal Abadimas Adi Buana Vol 9 No 02 (2026): Jurnal Abadimas Adi Buana
Publisher : LPPM Universitas PGRI Adi Buana Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36456/abadimas.v9.i02.a10808

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan ibu-ibu PKK Desa Janti, Kabupaten Sidoarjo dalam mengolah daun kelor menjadi produk pangan sehat berupa mie daun kelor. Kelor (Moringa oleifera) merupakan tanaman lokal yang kaya zat gizi dan berpotensi sebagai bahan pangan fungsional. Pelatihan dilaksanakan pada bulan Agustus–September 2025 dan diikuti oleh 20 peserta. Metode pelaksanaan meliputi tahapan persiapan, sosialisasi, pelatihan, serta evaluasi pengetahuan melalui pre-test dan post-test. Hasil evaluasi menunjukkan adanya peningkatan nilai rata-rata peserta dari 56,4 pada pre-test menjadi 87,2 pada post-test, atau terjadi peningkatan sebesar 54,6%. Peningkatan tertinggi terjadi pada aspek keterampilan pembuatan mie daun kelor. Kegiatan ini terbukti efektif dalam meningkatkan literasi gizi, keterampilan pengolahan pangan, serta kesadaran akan peluang usaha berbasis bahan lokal, sehingga pelatihan ini dapat menjadi model pemberdayaan masyarakat desa dalam mendukung ketahanan pangan keluarga dan ekonomi rumah tangga.
Co-Authors ., Intashofal Amilah, Susie Andriani, Vivin Arif Yachya Augustien, Nora Aulani F. Fithroh Binawati, Diah Karunia Choirunnisa, Nanis Dewi, Astuti Diah Karunia Binawati Diah Karunia Binawati ersanto, Ersanto Ervina Ervina Evarina, Melinda Yuti F, Eka p F. Deru Dewanti Febrianti Febrianti Febrianti, D. Febrianti, D. Gusti Azzahra Widiaura Hasanah, Waqiatul Hidayati, Isnainy Hidayati, Isnainy Holki, Nurul Islam Fi Karunia Binawati, Diah Kusuma, Novi Dwi Laia, Sanima M, Apolonia M, Apolonia M. Saftian Andreanto Masruroh MASRUROH Medistriani, DesyaTresty Ngadiani, Ngadiani Nova Triani Nugroho , Ganda Adi Nur Syamsiatul Fajar, Nur Syamsiatul Pratama, Yoga Bagas Pungky Slamet Wisnu Kusuma Purity Sabila Ajiningrum Rachmatin, Diaz P Rahayu, Asti Rahayu Rojib, Rojib Rojib, Rojib Sanima Laia Slamet Wisnu Kusuma, Pungky Sudjarwo Sudjarwo susie Amilah TATANG SOPANDI Tony Susilo Wibowo Umi Nurhayati, Umi Vivin Andriani Wahyudi, R. T. Wahyudi, R. T. Wiranata, Feri WK, Pungky Slamet Yani, Alfian Dwi Cipta