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All Journal Folia Medica Indonesiana Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Abadimas Adi Buana Jurnal Penamas Adi Buana Wahana : Tridarma Perguruan Tinggi Medical and Health Science Journal Bioscientist : Jurnal Ilmiah Biologi Seminar Nasional Hasil Riset dan Pengabdian (SNHRP) Stigma : Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa Lux Mensana: Journal of Scientific Health
Sukarjati Sukarjati, Sukarjati
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Novel Therapeutic Potential of 'Ambon' Banana and Green Bean Sprout Extracts in Mitigating Cigarette Smoke-Induced Testicular Oxidative Stress and Sperm Quality Impairment Sukarjati, Sukarjati; Yani, Alfian Dwi Cipta; Kusuma, Novi Dwi; Wiranata, Feri
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.5755

Abstract

Cigarette smoke exposure generates free radicals, causing oxidative stress and adversely affecting reproductive organs, resulting in reduced sperm quality. The aim of this study was to investigate the therapeutic benefits of 'Ambon' banana and green bean sprout extracts, either individually or in combination, in mitigating testicular MDA levels and preserving spermatogenic cells in rats exposed to cigarette smoke. 35 Male rats, aged 3 months, were divided into five groups: negative control, positive control (cigarette smoke-exposed), P1 (cigarette smoke + 'Ambon' banana extract), P2 (cigarette smoke + green bean sprout extract), and P3 (cigarette smoke + 'Ambon' banana and green bean sprout extracts). Cigarette smoke exposure was administered at 4 sticks/day/group for 30 days, and extracts were orally administered using a sonde for the same duration. The study showed that 'Ambon' banana extract, green bean sprout extract, and their combination significantly reduced testicular MDA levels, increased the number of spermatogonia, spermatocytes, spermatids, testicular weight, diameter of seminiferous tubules, and the number of mouse Leydig cells in rats. This study highlights the potential therapeutic effects of 'Ambon' banana and green bean sprout extracts as protective agents against cigarette smoke-induced testicular oxidative stress and sperm quality impairment.
Enhancing Spermatozoa Quality and Spermatogenic Cell in Hyperglycemic Mice (Mus musculus L.) with Sambung Nyawa (Gynura procumbens) and Mahagany (Swietenia mahagoni jacq) extracts, Alone and in Combination Sukarjati; Nurhayati, Umi; Medistriani, DesyaTresty
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7180

Abstract

This study aimed to investigate the effect of the ethanol extract of sambung nyawa (Gynura procumbens) leaves, mahogany (Swietenia mahagoni jacq) seeds, and their combination on the motility, viability, morphology, and concentration of spermatozoa, spermatogonia, spermatocytes, and spermatids in hyperglycemic mice induced with 10% dextrose. A total of 48 male mice were used as experimental animals, and the extracts were administered orally for 35 days. On day 36, spermatozoa and testicular tissues were collected for analysis. The results showed that the extracts at various doses had the potential to reduce blood glucose levels and improve the motility, viability, morphology, and concentration of spermatozoa and germ cells in hyperglycemic mice. Result:The combination of sambung nyawa leaves and mahogany seeds at a dose of 150:150 mg/kg BW was found to be the most effective in reducing blood sugar levels and improving sperm parameters. Therefore, these extracts have the potential to be used as alternative herbal ingredients with similar effects to metformin in reducing diabetes and improving fertility.
The Effect of Potassium Dosage and Branch-Pruning on the Growth and Yield of Butternut Squash (Cucurbita moschata Durch) Dewanti, F. Deru; Augustien, Nora; Triani, Nova; Holki, Nurul Islam Fi; Sukarjati
Jurnal Penelitian Pendidikan IPA Vol 10 No 9 (2024): September
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i9.7565

Abstract

Butternut squash (C. moschata) is a honey squash plant (Cucurbita moschata Durch) which is an introduced plant from the lowlands of South America and has adapted to Indonesia. The research was carried out in the planting area of Kampung Labu Madu, Toyoresmi Village, Ngasem District, Kediri Regency from January to June 2021. Potassium is among major nutrients needed by plants in their growth and development.  Potassium fertilization can help plants improve the regulation of photosynthetic mechanisms, carbohydrate translocation, protein synthesis This research was a two-factor factorial experiment prepared using a Split Plot Design. The first factor (main plot) was the branch pruning (P) treatment which consisted of 2 levels and the second factor (subplot) was the dose of KCl (K) fertilizer which consisted of 4 levels. The results of the research showed that there was a real influence in the treatment without pruning on the parameters of the number of male flowers and on the parameter of fruit weight with the best treatment being pruning, whereas for the treatment the dose of KCl fertilizer had a real effect on the parameters.
The Impact of Smoking on Sperm Quality and the DAZ Gene Sukarjati; Choirunnisa, Nanis; Fajar, Nur Syamsiatul
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.9213

Abstract

Indonesia's male population is mostly smokers. The chemical content in cigarettes can reduce the quality of spermatogenesis, where spermatogenesis is influenced by the DAZ gene (deleted in Azoospermia). This study aims to analyze sperm quality in light, moderate, and heavy smokers in 3 age groups and analyze the presence of the DAZ gene in heavy smokers. This study used 180 samples divided into three age groups, namely men aged 26-30 years, 31-35 years, and 36-40 years. Each age group recorded the number of cigarettes consumed per day (light, moderate, and heavy smokers) and examined the quality of sperm. While the DAZ gene analysis used 10 samples of heavy male smokers, aged 25-40 years. Sperm quality analysis was based on the WHO laboratory manual for human semen testing, while DAZ gene analysis used the PCR method. Sperm quality data were analyzed using Kruskal-Wallis, while the DAZ gene was analyzed qualitatively descriptively by analyzing electrophoresis photos. The results of this study can be concluded that daily cigarette consumption in the age group affects sperm quality and in heavy smokers, there are 10% who experience deletions in the DAZ gene. Thus cigarettes can reduce spermatozoa quality.
Potential of Combined Insulin Leaf (Smallanthus sonchifolius) and Noni Fruit (Morinda citrifolia L.) Extracts in Reducing Blood Glucose Level and Spermatogenic Cells Improvement in Diabetic Mice Sukarjati; Syahputra, Aldi
Jurnal Penelitian Pendidikan IPA Vol 11 No 5 (2025): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i5.11174

Abstract

Elevated blood glucose levels are a hallmark of diabetes mellitus, which can lead to reproductive disorders, particularly affecting spermatogenic cells. This study aimed to evaluate the effectiveness of insulin leaf extract, noni fruit extract, and their combinations in reducing blood glucose levels and their impact on the number of spermatogenic cells in diabetic mice. A total of 36 male mice were divided into 12 treatment groups, including a normal control group, a negative control group (induced with alloxan 120 mg/KgBW), a positive control group (alloxan + metformin), and nine treatment groups with various doses of individual and combined extracts. Blood glucose levels were measured using Accu-Chek, and testicular histology was prepared following laboratory standards. Data were analyzed using the ANOVA test followed by the Duncan post hoc test. Results showed that the combination of insulin leaf extract at 150 mg/KgBW/day and noni fruit extract at 62.5 mg/KgBW/day was the most effective in reducing blood glucose levels. Additionally, the combination of 125 mg/KgBW insulin leaf extract and 50 mg/KgBW noni fruit extract significantly increased the number of spermatogenic cells. Thus, the combination of insulin leaf and noni fruit extracts shows potential as a natural antidiabetic therapy and for restoring spermatogenic cell function in diabetic conditions.
Potential of Combined Curcuma zedoaria and Piper crocatum Leaf Extracts as Natural Male Antifertility Agents: an Experimental Study in Mice Sukarjati; Sari, Mitha Novia
Jurnal Penelitian Pendidikan IPA Vol 11 No 10 (2025): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i10.12380

Abstract

Curcuma zedoaria and Piper crocatum are indigenous Indonesian herbal plants containing various bioactive compounds. C. zedoaria is known to contain curcumin, tannins, saponins, and flavonoids, while P. crocatum contains piperine, tannins, saponins, flavonoids, alkaloids, and triterpenoids. This study aimed to investigate the effects of white turmeric extract, red betel leaf extract, and their combination on the spermatogenic cells of male mice. Extracts were obtained using the maceration method. A total of 30 male mice, aged 3 months and weighing 25–30 grams, were randomly divided into four treatment groups with three replications each: control, white turmeric extract, red betel extract, and a combination of both extracts. The extracts were administered orally at doses of 50, 100, and 150 mg/kg BW for individual extracts, and 25:25, 50:50, and 75:75 mg/kg BW for the combined treatment, over 35 days. Histological sections of the testis were prepared following standard laboratory protocols. One-way ANOVA showed a significant decrease (p < 0.05) in all cell types in treated groups, with the most substantial reduction observed at the 75:75 mg/kg BW combination dose. The conclusion of this study is that the combined extract of C. zedoaria and P. crocatum has the potential as an antifertility
Co-Authors ., Intashofal Amilah, Susie Andriani, Vivin Arif Yachya Augustien, Nora Aulani F. Fithroh Binawati, Diah Karunia Choirunnisa, Nanis Dewi, Astuti Diah Karunia Binawati Diah Karunia Binawati ersanto, Ersanto Ervina Ervina Evarina, Melinda Yuti F, Eka p F. Deru Dewanti Febrianti Febrianti Febrianti, D. Febrianti, D. Gusti Azzahra Widiaura Hasanah, Waqiatul Hidayati, Isnainy Hidayati, Isnainy Holki, Nurul Islam Fi Karunia Binawati, Diah Kusuma, Novi Dwi Laia, Sanima M, Apolonia M, Apolonia M. Saftian Andreanto Medistriani, DesyaTresty Ngadiani, Ngadiani Nugroho , Ganda Adi Nur Syamsiatul Fajar, Nur Syamsiatul Pratama, Yoga Bagas Pungky Slamet Wisnu Kusuma Purity Sabila Ajiningrum Rahayu, Asti Rahayu Rojib, Rojib Rojib, Rojib Sanima Laia sari, mitha novia Slamet Wisnu Kusuma, Pungky Sudjarwo Sudjarwo susie Amilah Syahputra, Aldi TATANG SOPANDI Tony Susilo Wibowo triani, nova Umi Nurhayati, Umi Vivin Andriani Wahyudi, R. T. Wahyudi, R. T. Wiranata, Feri WK, Pungky Slamet Yani, Alfian Dwi Cipta