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Antioxidant Testing and Identification of Bioactive Compounds in Ethanol Extract of Propolis from Various Locations in Indonesia using LCMS-QTOF Mulyati, Ade Heri; Alawiyah, Hanis; Marom, Muhamad Fathul; Warnasih, Siti
Chimica et Natura Acta Vol 12, No 3 (2024)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v12.n3.53081

Propolis produced by Trigona sp. stingless bees contains various bioactive substances including alkaloids, flavonoids, polyphenols, saponins, steroids, and terpenoids. The geographical origin of propolis production can influence its composition of secondary metabolites. This study aimed to qualitatively analyze ethanol extracts of propolis from Bintan, Lampung, and Makassar, and evaluate their phenolic and flavonoid contents, as well as antioxidant activities. The extraction method employed kinetic maceration with continuous stirring over 24 hours, using 70% ethanol as the solvent. Additionally, antioxidant activity was assessed using the DPPH method. The qualitative phytochemical analysis revealed that all three ethanol extracts contained alkaloids, flavonoids, and polyphenols. Saponin compounds were uniquely identified in the Makassar ethanol extract. Furthermore, antioxidant activity tests indicated significant potential in all three propolis extracts. These findings highlight the potential of Trigona sp. Stingless bee propolis as a valuable source of bioactive compounds beneficial for human health.

Penerapan Sistem Irigasi Tetes untuk Memaksimalkan Penggunaan Air bagi Petani Desa Kalong Liud Fatimah, Siti; Sutanto, Sutanto; Sutoyo, Muhammad Rayhan; Zahra, Mutiara; Mulyati, Ade Heri; Warnasih, Siti
Jurnal Abdimas Kartika Wijayakusuma Vol 5 No 3 (2024): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v5i3.548

Tim PPK Ormawa HIMASKA melakukan penerapan sistem irigasi tetes yang akan membantu memenuhi kebutuhan air tanaman selama musim kemarau dengan menjaga penggunaan air secara efisien di Desa Kalong Liud di mana sistem ini akan meningkatkan pemanfaatan unsur hara tanah, mengurangi tekanan air terhadap tanah, mempercepat adaptasi bibit, dan meningkatkan keberhasilan tumbuh tanaman. Selain itu, sistem irigasi tetes dapat mencapai tingkat efisiensi penggunaan air yang lebih tinggi. Metode yang dilakukan dalam kegiatan ini meliputi survei, pembentukan kelompok masyarakat, sosialisasi serta edukasi, pelaksanaan, dan evaluasi proses irigasi. Air dari toren SPAH dengan volume 1000 liter sebelum dimanfaatkan untuk irigasi tetes hanya dapat dipakai untuk tiga kali penyiraman, tetapi setelah menggunakan sistem irigasi tetes air dari SPAH dengan volume 1000 liter tersebut dapat dipakai untuk lima sampai enam kali penyiraman. Dengan begitu, dapat disimpulkan bahwa sistem ini sangat layak untuk diterapkan karena menekan penggunaan air untuk kebutuhan irigasi sehingga dapat menjadi solusi bagi para petani pada saat musim kemarau.

Efektivitas Ekstrak Daun Air Mata Pengantin (Antigonon Leptopus) dalam Penurunan Jumlah Bakteri Coliform di Air Sungai Megan, Aspasia Asprila; Fathurrahman, Muhammad; Mulyati, Ade Heri
Jurnal Zarah Vol. 12 No. 2 (2024): October, 2024
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/1gn7fv41

Air Sungai Cileungsi rentan tercemar pencemar biologis berupa bakteri dari limbah industri dan domestik, salah satunya adalah bakteri coliform. Bakteri coliform dalam sungai dapat membawa resiko kesehatan bagi warga tinggal di sekitarnya, sehingga perlu ditambahkan disinfektan untuk mengurangi jumlah bakteri dalam air. Daun tumbuhan air mata pengantin (Antigonon leptopus) telah dilaporkan memiliki sifat antibakteri sehingga dapat digunakan sebagai disinfektan air alternatif. Senyawa bioaktif Grosvenorine, Isoaloeresin D, dan Kaempferitrin terdeteksi oleh LC-MS/MS dalam ekstrak metanolik daun air mata pengantin dan ketiga senyawa tersebut telah dilaporkan memiliki sifat antibakteri dari penelitian-penelitian yang ada sebelumnya. Hasil penelitian ini memperlihatkan bahwa penambahan ekstrak dapat mengurangi jumlah bakteri coliform dengan optimal mulai dari 6 jam dengan konsentrasi 10% dari total volume air, dan pada waktu 24 jam dapat menurunkan jumlah bakteri hingga 100%.

Identification of Strobilanthes crispa from Its Related Plant Using Thin-layer Chromatography Fingerprint Analysis Hasanah, Uswatun; Irene, Angelina; Mulyati, Ade Heri; Batubara, Irmanida; Rafi, Mohamad; Rohaeti, Eti
Majalah Obat Tradisional Vol 30, No 2 (2025)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.95008

Strobilanthes crispa known as keji beling in Indonesia, belong to the Acanthaceae family. Thin layer chromatography (TLC) fingerprint pattern analysis is an excellent technique for evaluating and controlling the quality of raw materials containing Strobilanthes crispa. TLC fingerprints can be used to analyze the fingerprint profiles of medicinal plants for identification, authentication, and discrimination from related plants. This study aimed to develop a TLC fingerprint analysis method to identify S. crispa for quality control. Eleven bands were effectively separated using a silica gel 60 F254 TLC plate with a mobile phase of dichloromethane, ethyl acetate, and chloroform at 16:3.2:0.8. The derivatization reagent used to detect the separated bands was 10% sulfuric acid at a wavelength of 366 nm. Validation of the TLC fingerprint analysis, which evaluated the robustness, stability, specificity, and precision, met these requirements. S. crispa can be distinguished by its TLC fingerprint from sirih hutan (Piper aduncum), a related plant with similar leaf morphology. This approach could be used to identify and authenticate of S. crispa from P. aduncum.

Conservation of Water Resources Around Rivers as an Effort to Empower the Community in Kalong Liud Village Effendi, Herlinda; Amelia, Nisa; Gymnasti, Alista Dwis; Sutanto; Mulyati, Ade Heri; Warnasih, Siti
Jurnal Pengabdian Masyarakat Inovatif Vol. 2 No. 2 (2024): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v2i2.124

Program Penguatan Kapasitas Kemahasiswaan team (PPK Ormawa) of Himpunan Mahasiswa Kimia (HIMASKA) of the Faculty of Mathematics and Science, Pakuan University has implemented a water resource conservation program to overcome floods and droughts that occur every year in Kalong Liud Village, Nanggung District, Bogor Regency, West Java Province. Water resource conservation with green ampel bamboo (Bambusa vulgaris Schrad. ex. J.C) uses a combination of vegetative and agronomic methods with the aim of maximizing erosion control and surface flow of the Cikaniki River. This program began with a survey of village locations, socialization and education to maximize the sustainability of the implementation of the program by holding pre-tests and post-tests for community groups and residents around the Cikaniki River. After that, green ampel bamboo was planted on the cleared and dug land 50 cm deep with a hole diameter of 15 cm. The growth of green ampel bamboo is monitored and evaluated once a week to find out how to optimize its growth. As a result, young leaves grow from bamboo books after 3 (three) weeks of planting and maintenance. In addition, the provision of education and scientific approaches can increase public understanding and awareness of climate change that can cause flood disasters.

Intensification of catfish cultivation at Pokdakan Baraya in Kotabatu Village, Bogor Regency Hasanah, Uswatun; Kusumawardani, Linda Jati; Syahputri, Yulian; Mulyati, Ade Heri; Widiastuti, Diana; Warnasih, Siti
Jurnal Pengabdian Masyarakat Inovatif Vol. 3 No. 1 (2025): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v3i1.154

Kotabatu Village, located in Bogor District, is home to several Fish Cultivator Groups (Pokdakan), one of which is Pokdakan Baraya, focused on cultivating catfish. Based on a focus group discussion conducted by the Pakuan University Implementation Team, Pokdakan Baraya faces challenges related to low income, high production costs, and the risks involved in traditional fish farming practices. In response, the team proposed the introduction of the Biofloc system for fish cultivation. The service methods included a comprehensive survey to assess the conditions and needs of the target community, followed by focus group discussions (FGD). The next phase involved outreach by the Pakuan University service team, employing knowledge-sharing methods, case studies, and training on the biofloc technology. To evaluate the impact, regular monitoring and evaluation processes were implemented, which included counseling, pond construction, seed distribution, nursery establishment, and intensive catfish rearing using the biofloc system. As a result of the biofloc system’s adoption, pond productivity increased by 1.8%, and the harvest time was reduced by 15 days. Additionally, the knowledge of Pokdakan Baraya members regarding fish cultivation technology grew by 60%, and their income saw an 81% increase from the sale of the catfish. The successful implementation of this system demonstrates significant improvements in the efficiency, productivity, and economic stability of the community.

Conservation of Water Resources Around Rivers as an Effort to Empower the Community in Kalong Liud Village Effendi, Herlinda; Amelia, Nisa; Gymnasti, Alista Dwis; Sutanto; Mulyati, Ade Heri; Warnasih, Siti
Jurnal Pengabdian Masyarakat Inovatif Vol. 2 No. 2 (2024): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v2i2.124

Program Penguatan Kapasitas Kemahasiswaan team (PPK Ormawa) of Himpunan Mahasiswa Kimia (HIMASKA) of the Faculty of Mathematics and Science, Pakuan University has implemented a water resource conservation program to overcome floods and droughts that occur every year in Kalong Liud Village, Nanggung District, Bogor Regency, West Java Province. Water resource conservation with green ampel bamboo (Bambusa vulgaris Schrad. ex. J.C) uses a combination of vegetative and agronomic methods with the aim of maximizing erosion control and surface flow of the Cikaniki River. This program began with a survey of village locations, socialization and education to maximize the sustainability of the implementation of the program by holding pre-tests and post-tests for community groups and residents around the Cikaniki River. After that, green ampel bamboo was planted on the cleared and dug land 50 cm deep with a hole diameter of 15 cm. The growth of green ampel bamboo is monitored and evaluated once a week to find out how to optimize its growth. As a result, young leaves grow from bamboo books after 3 (three) weeks of planting and maintenance. In addition, the provision of education and scientific approaches can increase public understanding and awareness of climate change that can cause flood disasters.

Intensification of catfish cultivation at Pokdakan Baraya in Kotabatu Village, Bogor Regency Hasanah, Uswatun; Kusumawardani, Linda Jati; Syahputri, Yulian; Mulyati, Ade Heri; Widiastuti, Diana; Warnasih, Siti
Jurnal Pengabdian Masyarakat Inovatif Vol. 3 No. 1 (2025): JPMI (Jurnal Pengabdian Masyarakat Inovatif)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jpmi.v3i1.154

Kotabatu Village, located in Bogor District, is home to several Fish Cultivator Groups (Pokdakan), one of which is Pokdakan Baraya, focused on cultivating catfish. Based on a focus group discussion conducted by the Pakuan University Implementation Team, Pokdakan Baraya faces challenges related to low income, high production costs, and the risks involved in traditional fish farming practices. In response, the team proposed the introduction of the Biofloc system for fish cultivation. The service methods included a comprehensive survey to assess the conditions and needs of the target community, followed by focus group discussions (FGD). The next phase involved outreach by the Pakuan University service team, employing knowledge-sharing methods, case studies, and training on the biofloc technology. To evaluate the impact, regular monitoring and evaluation processes were implemented, which included counseling, pond construction, seed distribution, nursery establishment, and intensive catfish rearing using the biofloc system. As a result of the biofloc system’s adoption, pond productivity increased by 1.8%, and the harvest time was reduced by 15 days. Additionally, the knowledge of Pokdakan Baraya members regarding fish cultivation technology grew by 60%, and their income saw an 81% increase from the sale of the catfish. The successful implementation of this system demonstrates significant improvements in the efficiency, productivity, and economic stability of the community.

In vitro antimicrobial screening of Manihot esculenta Sao Pedro Petro Extract and Identification of Active Compounds Widiastuti, Diana; Sinaga, Siska Elisahbet; Sofian, Yayan; Mulyati, Ade Heri; Warnasih, Siti; Sadjarwa, Boedi; Dhiandani, Sri Boedi; Herlina, Eka; Triastinurmiatiningsih, Triastinurmiatiningsih; Agustine, Dine
Chimica et Natura Acta Vol 12, No 1 (2024)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v12.n1.50154

Sao Pedro Petro cassava tubers (Manihot esculenta Ctro) are one of the cassava varieties, generally cultivated in tropical and subtropical areas. This plant has the potential to contain antimicrobial bioactive compounds. This research aimed to demonstrate the antibacterial activity of each fraction and subsequently identify the specific active fraction for in-depth analysis of its bioactive compounds. The evaluation of antimicrobial activity and the identification of bioactive compounds were conducted using the well-diffusion method and UPLC-QTOF MS (Ultra Performance Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry). Results indicated that the ethanol, n-hexane, and n-butanol fractions exhibited no inhibitory effects on the tested microorganisms. In contrast, the ethyl acetate fraction displayed the highest level of antimicrobial activity (Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 27853, and Candida albicans ATCC 1023). Further analysis of the ethyl acetate fraction revealed key bioactive compounds, including Coixol (phenol-group), Trigonelline and 2,6-Dimethyl quinoline (alkaloid-group), and Chebuloside-II (terpenoid-group). In summary, this pioneering research represents the first-ever exploration of the antimicrobial potential of cassava tubers, specifically focusing on the Sao Pedro Petro variety. The study not only underscores the antimicrobial properties of Manihot esculenta tubers but also identifies specific bioactive compounds within them, providing valuable insights into their potential therapeutic applications

Antioxidant Potential and Active Compound Identification of Hylocereus costaricensis and Hylocereus undatus Peel Extracts using LC-MS/MS Mulyati, Ade Heri; Nurmayani, Reni; Widiastuti, Diana; Warnasih, Siti; Sinaga, Siska Elisahbet
Chimica et Natura Acta Vol 13, No 2 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n2.61186

The object of this research was to determine the antioxidant potential and identify active compounds in the peel extracts of red dragon fruit (Hylocereus costaricensis) and white dragon fruit (Hylocereus undatus). Phytochemical tests were conducted to identify flavonoids, alkaloids, tannins, coumarins, and saponins. The antioxidant activity was assessed using the DPPH assay, while vitamin C content was quantified using UV-Vis spectrophotometry, and bioactive antioxidant compounds were identified through LC-MS/MS spectrophotometry. The results demonstrated that phytochemical screening revealed the presence of flavonoids and alkaloids in both extracts. Antioxidant activity assays indicated that the red dragon fruit peel extract exhibited an IC50 of 32.7 ppm (very active), while the white dragon fruit peel extract had an IC50 of 50.2 ppm (active), with the vitamin C control showing an IC50 of 11.90 ppm. The red dragon fruit peel extract had the highest vitamin C content (1.45%) compared to the white dragon fruit peel (1.04%). LC-MS/MS analysis identified several organic compounds, including the flavonoid isoliquiritigenin and alkaloid compounds such as aporphine, melosmine, 3-carbamoyl-1-(2-deoxy-beta-D-erythro-pentofuranosyl)-4,5-dimethylpyridinium, and alkaloid group (6-Amino-5-{[2(diethylamino) ethyl]amino}-1-propyl -2,4(1H,3H)-pyrimidinedione). These findings suggest that both red and white dragon fruit peel extracts possess significant antioxidant potential, with red dragon fruit peel demonstrating superior activity. This study can be used for further research on food technology in producing food products that are rich in nutrients.

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