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Journal : Helium: Journal of Science and Applied Chemistry

Addition of Maltodextrin Concentration to Anthocyanin Characteristics and Levels in the Manufacture of Natural Dyes from the Skin of Red Dragon Fruit (Hylocereus polyrhizus) Eka Herlina; Diana Widiastuti; Fatwa Rachmawati Annissa
Helium: Journal of Science and Applied Chemistry Vol 1, No 1 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.082 KB) | DOI: 10.33751/helium.v1i1.2948

Abstract

Synthetic dyes in food products can cause health problems, making some people reduce such products' consumption. Alternatively, instead of avoiding consumption, people can do better by switching to natural dyes in food processing. One possible solution is to use the red dragon fruit's skin (hylocereus polyrhizus), containing anthocyanin pigments of the cyanidin-3-glucoside type. The purpose of this study is to propose the use of red dragon fruit's skin as a natural dye and to determine the characteristics, anthocyanin levels, and shelf life of red dragon fruit peel dye. The making of red dragon fruit peel dye was done by first extracting it with a maceration technique. After that, maltodextrin was added to the filtrate while the drying temperature was carried out at 28°C and 13°C. The results showed that natural dyes with a concentration of 30% maltodextrin at 13° C were the selected dyes and had the best characteristics. The total yield analysis resulted was 11.30%; pH measurement of 4.24; water content of 1.03%; solubility of 98.08%; color intensity L value of 59.41; a value of 33.44; and the b value of -7.78. The results obtained from the analysis of anthocyanin levels were 15.0290 ppm. The total plate number test obtained is 2.1 x 102 colonies/gram with a storage life of 282 days.
THE EFFECT OF FORTIFICATION OF BRANDS AND CHITOSAN ON TEMPEH ON FIBER LEVELS AND PROBIOTIC BACTERIA GROWTH Nunung Kurniasih; Farah Dinna; Vina Amalia; Diana Widiastuti
Helium: Journal of Science and Applied Chemistry Vol 1, No 2 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.827 KB) | DOI: 10.33751/helium.v1i2.4511

Abstract

Tempeh is a food made from soybean seeds processed through fermentation using Rhizopus sp. This processed food in the form of tempeh contains various nutrients that the body needs such as protein, fat, carbohydrates, and minerals but does not contain enough fiber. Therefore, fortification of rice bran and chitosan was carried out on tempeh. Rice bran is a by-product of rice milling which has a high fiber content. While chitosan is chitin that has removed the acetyl group. Chito-oligosaccharides (COS) contained in chitosan can act as natural prebiotics, preservatives, antimicrobials, lower cholesterol levels and are immunostimulants. The purpose of this study was to determine the fiber content and growth of the probiotic bacteria Lactobacillus acidophilus in soybean tempeh (A), rice bran (B), chitosan (C), the ratio of soybean tempeh: rice bran (6: 3) + 2% chitosan (D) and the ratio of soybean tempeh: bran (6: 2) + 2% chitosan (E). The method used for the analysis of fiber content was gravimetric, while the growth of probiotic bacteria Lactobacillus acidophilus was used to determine the potential of prebiotics contained in samples A, B, C, D, and E. The results obtained in this study were fortification of rice bran and chitosan in tempeh had high levels of fiber and the number of probiotic bacteria cells was higher than soybean tempeh. The higher the bran added, the higher the fiber content and the number of probiotic bacterial cells. while the growth of probiotic bacteria Lactobacillus acidophilus was used to determine the potential of prebiotics contained in samples A, B, C, D, and E. The results obtained in this study were bran and chitosan fortifications in tempeh had higher fiber content and cell counts of probiotic bacteria than soybean tempeh. The higher the bran added, the higher the fiber content and the number of probiotic bacterial cells. while the growth of probiotic bacteria Lactobacillus acidophilus was used to determine the potential of prebiotics contained in samples A, B, C, D, and E. The results obtained in this study were bran and chitosan fortifications in tempeh had higher fiber content and cell counts of probiotic bacteria than soybean tempeh. The higher the bran added, the higher the fiber content and the number of probiotic bacterial cells.
Antimicrobial Activity of The Ethanol Extract of Coffea canephora L. Seeds Against Staphylococcus aureus and Propionibacterium acnes Siska Elisahbet Sinaga; Matius Paundanan; Maharani Farah Dhifa Dg Masikki; Diana Widiastuti
Helium: Journal of Science and Applied Chemistry Vol 3, No 1 (2023): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v3i1.7512

Abstract

Acne is a skin disease that causes humans to lack confidence in carrying out activities. Besides being sick, acne worsens the beauty of human skin. The cause of acne is gram-positive bacteria, namely Staphylococcus aureus and Propionibacterium acnes. Many studies reported that Coffea canephora L can inhibit the growth of bacteria. The objective of this study is to establish the minimum inhibitory concentration (MIC) of the ethanol extract of robusta coffee seeds in inhibiting the growth of Staphylococcus aureus and Escherichia coli. The ethanol extract of C. canephora L seed was obtained by maceration method using 96% ethanol. The extract obtained was evaluated for its antibacterial activity using the agar diffusion method with perforation techniques. The extract was divided into five various concentrations (50%, 25%, 12.5%, 6.25%, and 3.125%). The results of this study indicated that the ethanol extract of coffee seeds with a concentration of 50% resulted in a wider diameter of the zone of inhibition of bacterial growth in S. aureus, 30.00 mm compared to P. acnes, 26.22 mm. The MIC of ethanol extract for S. aureus at 3.00%  while P. acnes was 6.00%.  In conclusion, the antibacterial effect of the ethanol extract of coffee seeds at 50% concentration was stronger against S. aureus bacteria than P. acnes. The greater the concentration of the extract, the wider the resulting inhibition zone.
Addition of Maltodextrin Concentration to Anthocyanin Characteristics and Levels in the Manufacture of Natural Dyes from the Skin of Red Dragon Fruit (Hylocereus polyrhizus) Eka Herlina; Diana Widiastuti; Fatwa Rachmawati Annissa
Helium: Journal of Science and Applied Chemistry Vol 1, No 1 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v1i1.2948

Abstract

Synthetic dyes in food products can cause health problems, making some people reduce such products' consumption. Alternatively, instead of avoiding consumption, people can do better by switching to natural dyes in food processing. One possible solution is to use the red dragon fruit's skin (hylocereus polyrhizus), containing anthocyanin pigments of the cyanidin-3-glucoside type. The purpose of this study is to propose the use of red dragon fruit's skin as a natural dye and to determine the characteristics, anthocyanin levels, and shelf life of red dragon fruit peel dye. The making of red dragon fruit peel dye was done by first extracting it with a maceration technique. After that, maltodextrin was added to the filtrate while the drying temperature was carried out at 28°C and 13°C. The results showed that natural dyes with a concentration of 30% maltodextrin at 13° C were the selected dyes and had the best characteristics. The total yield analysis resulted was 11.30%; pH measurement of 4.24; water content of 1.03%; solubility of 98.08%; color intensity L value of 59.41; a value of 33.44; and the b value of -7.78. The results obtained from the analysis of anthocyanin levels were 15.0290 ppm. The total plate number test obtained is 2.1 x 102 colonies/gram with a storage life of 282 days.
The Effect of Fortification of Brands and Chitosan on Tempeh on Fiber Levels and Probiotic Bacteria Growth Nunung Kurniasih; Farah Dinna; Vina Amalia; Diana Widiastuti
Helium: Journal of Science and Applied Chemistry Vol 1, No 2 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v1i2.4511

Abstract

Tempeh is a food made from soybean seeds processed through fermentation using Rhizopus sp. This processed food in the form of tempeh contains various nutrients that the body needs such as protein, fat, carbohydrates, and minerals but does not contain enough fiber. Therefore, fortification of rice bran and chitosan was carried out on tempeh. Rice bran is a by-product of rice milling which has a high fiber content. While chitosan is chitin that has removed the acetyl group. Chito-oligosaccharides (COS) contained in chitosan can act as natural prebiotics, preservatives, antimicrobials, lower cholesterol levels and are immunostimulants. The purpose of this study was to determine the fiber content and growth of the probiotic bacteria Lactobacillus acidophilus in soybean tempeh (A), rice bran (B), chitosan (C), the ratio of soybean tempeh: rice bran (6: 3) + 2% chitosan (D) and the ratio of soybean tempeh: bran (6: 2) + 2% chitosan (E). The method used for the analysis of fiber content was gravimetric, while the growth of probiotic bacteria Lactobacillus acidophilus was used to determine the potential of prebiotics contained in samples A, B, C, D, and E. The results obtained in this study were fortification of rice bran and chitosan in tempeh had high levels of fiber and the number of probiotic bacteria cells was higher than soybean tempeh. The higher the bran added, the higher the fiber content and the number of probiotic bacterial cells. while the growth of probiotic bacteria Lactobacillus acidophilus was used to determine the potential of prebiotics contained in samples A, B, C, D, and E. The results obtained in this study were bran and chitosan fortifications in tempeh had higher fiber content and cell counts of probiotic bacteria than soybean tempeh. The higher the bran added, the higher the fiber content and the number of probiotic bacterial cells. while the growth of probiotic bacteria Lactobacillus acidophilus was used to determine the potential of prebiotics contained in samples A, B, C, D, and E. The results obtained in this study were bran and chitosan fortifications in tempeh had higher fiber content and cell counts of probiotic bacteria than soybean tempeh. The higher the bran added, the higher the fiber content and the number of probiotic bacterial cells.
Determination of Anti-Browning Agents in The Manufacture of Flour from Kepok Banana Skin Waste Nuraeni, Farida; Widiastuti, Diana; Herlina, Eka; Benedicta, Glisten Madeleine
Helium: Journal of Science and Applied Chemistry Vol 4, No 1 (2024): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v4i1.10350

Abstract

Kepok banana is one of the bananas which is a horticultural commodity, easy to cultivate and has high production value in Indonesia. The high production of bananas cannot be separated from the banana peel waste produced. The effort made is to process Kepok banana peels into flour. One of the problems that occurs is that bananas experience a browning reaction. This research aims to reduce the effects of browning reactions by determining anti-browning substances and optimum concentrations in the process of making kepok banana peel flour, so that it can be used as a food ingredient. Making kepok banana peel flour begins with preparing a sample of kepok banana peel and an anti-browning solution. The samples were immersed in each solution, namely Na2S2O5, C₆H₈O₆, and C₆H₈O₇ with a concentration of 0.1 – 0.2%. Control samples were prepared without soaking. Then it is dried, mashed and sifted until it becomes flour. Organoleptic tests and white degree tests were carried out on 6 samples and 1 control. Selected samples are then subjected to chemical tests in the form of proximate and minerals. The results of the organoleptic test and white degree test showed that sample T4, namely soaking using 0.2% Ascorbic Acid anti-browning solution, was the sample selected by the panelists and had the highest white degree, namely 53,26. The results of the proximate and mineral tests for T4 flour samples compared to T1 flour (control) showed that the T4 flour samples had proximate and mineral content values that largely met the standards for wheat flour according to SNI 3751:2009. Only the ash content exceeds the SNI standard, this can be caused by the high iron mineral content.