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All Journal Biota BIO-SITE |BIOLOGI Sains Terapan Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Oedjijono Oedjijono, Oedjijono
Universitas Jendral Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Kemampuan Bakteri Halotoleran dari Sedimen Mangrove Pantai Logending dalam Pelarutan Fosfat dan Penambatan Nitrogen Ramadani, Adib; Oedjijono, Oedjijono; Pramono, Hendro; Pratiwi, Meyta
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.14854

Abstract

Lingkungan mangrove dapat menjadi sumber yang baik untuk mendapatkan bakteri halotoleran yang berpotensi sebagai agensia pupuk hayati dengan kemampuan melarutkan fosfat dan menambat N2 bebas. Tujuan penelitian ini adalah menguji toleransi isolat bakteri asal sedimen mangrove terhadap salinitas, menguji kemampuan isolat bakteri halotoleran dalam pelarutan fosfat dan penambatan nitrogen bebas, dan karakterisasi fenetik isolat bakteri halotoleran terpilih. Hasil penelitian menunjukkan bahwa dari 43 isolat yang diteliti, sebanyak 29 isolat toleran terhadap NaCl 5-7%. Dari 29 isolat, sebanyak 11 isolat bakteri halotoleran (>5% NaCl) diketahui mampu melarutkan fosfat dan menambat nitrogen dengan tiga isolat terpilih (LG36, LG60, dan LG66). Ketiga isolat tersebut menunjukkan efisiensi pelarutan fosfat masing-masing 125, 123,72, dan 126,92; dan kemampuan penambatan nitrogennya berturut-turut 38,02 ppm; 28,4 ppm; dan 51,93 ppm. Identitas ketiga isolat terpilih menunjukkan bahwa isolat LG60 merupakan spesies anggota genus Rhizobium, sedangkan isolat LG36 dan LG66 belum dapat diidentifikasi pada level genus. Kata kunci: fiksasi nitrogen, halotoleran, mangrove, pelarutan fosfat
Petroleum Degradation by Bacteria Explored from Logending Mangrove Sediments Apriliani, Herlina; Pramono, Hendro; Oedjijono, Oedjijono; Widodo, Wimbuh Tri; Kristianto, Sonny; Putri, Rury Eryna; Unsunnidhal, Lalu
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.29245

Abstract

Oil spills resulting from shipping activities, tanker-based oil transportation, and fuel oil usage can cause coastal pollution, particularly in sensitive ecosystems such as mangroves. More than 90% of petroleum consists of hydrocarbons with complex carbon chain structures, making them difficult to decompose. Biological remediation using microorganisms offers a promising alternative for pollution mitigation, as microbes can degrade petroleum components and oxidizing hydrocarbons. This study aimed to evaluate the petroleum-degrading ability of selected bacterial isolates obtained from mangrove sediments at Logending Beach. The research employed experimental and survey methods. The primary parameter measured was Total Petroleum Hydrocarbons (TPH), while supporting parameters included pH and bacterial population density. The study consisted of several stages, including bacterial isolation, screening, and evaluation of the petroleum degradation capacity of selected isolates. The results identified two potential bacterial isolates capable of degrading crude oil. Isolate LG62 exhibited a degradation efficiency of 71.40%, while isolate LG105 showed a degradation efficiency of 57.10%. Petroleum concentrations of 2% (v/v) and 5% (v/v) were degraded more effectively than higher concentrations. Overall, the two bacterial isolates (LG62 and LG105) from Logending mangrove sediments demonstrated significant potential as bioremediation agents for petroleum hydrocarbon contamination.
Co-Authors Amalia, Dwi Ayu Lutfiani Andani, Ajeng Putri Retno anti, Monica Widi Apriliani, Herlina Ardhini R Maharning Aziz, Saefudin Daniel Joko Wahyono Dini Ryandini Dyah Fitri Kusharyati ELLY PROKLAMASININGSIH Fathurrohim, Ramadi Habib Hendro Pramono Isnasari, Azma Nurizqi nurainy, niharoh Nurmalasari, Ade Pratiwi, Meyta Ramadani, Adib Rony, Zahara Tussoleha Rury Eryna Putri Slamet Santoso Sodik Wuryanto, Sodik Sonny Kristianto Unsunnidhal, Lalu Widodo Widodo Wimbuh Tri Widodo