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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota BIO-SITE |BIOLOGI Sains Terapan Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia Journal of Biomedical Sciences and Health
Oedjijono Oedjijono, Oedjijono
Universitas Jendral Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Kemampuan Bakteri Halotoleran dari Sedimen Mangrove Pantai Logending dalam Pelarutan Fosfat dan Penambatan Nitrogen Ramadani, Adib; Oedjijono, Oedjijono; Pramono, Hendro; Pratiwi, Meyta
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.14854

Abstract

Lingkungan mangrove dapat menjadi sumber yang baik untuk mendapatkan bakteri halotoleran yang berpotensi sebagai agensia pupuk hayati dengan kemampuan melarutkan fosfat dan menambat N2 bebas. Tujuan penelitian ini adalah menguji toleransi isolat bakteri asal sedimen mangrove terhadap salinitas, menguji kemampuan isolat bakteri halotoleran dalam pelarutan fosfat dan penambatan nitrogen bebas, dan karakterisasi fenetik isolat bakteri halotoleran terpilih. Hasil penelitian menunjukkan bahwa dari 43 isolat yang diteliti, sebanyak 29 isolat toleran terhadap NaCl 5-7%. Dari 29 isolat, sebanyak 11 isolat bakteri halotoleran (>5% NaCl) diketahui mampu melarutkan fosfat dan menambat nitrogen dengan tiga isolat terpilih (LG36, LG60, dan LG66). Ketiga isolat tersebut menunjukkan efisiensi pelarutan fosfat masing-masing 125, 123,72, dan 126,92; dan kemampuan penambatan nitrogennya berturut-turut 38,02 ppm; 28,4 ppm; dan 51,93 ppm. Identitas ketiga isolat terpilih menunjukkan bahwa isolat LG60 merupakan spesies anggota genus Rhizobium, sedangkan isolat LG36 dan LG66 belum dapat diidentifikasi pada level genus. Kata kunci: fiksasi nitrogen, halotoleran, mangrove, pelarutan fosfat
GENOTYPIC AND PHENOTYPIC CHARACTERIZATION OF Alcaligenes javaensis JG3 POTENTIAL AS AN EFFECTIVE BIODEGRADER Ethica, Stalis Norma; Oedjijono, Oedjijono; Semiarti, Endang; Widada, Jaka; Raharjo, Tri Joko
BIOTROPIA Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.448 KB) | DOI: 10.11598/btb.2018.25.1.583

Abstract

Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenes sp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenes sp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader.
Petroleum Degradation by Bacteria Explored from Logending Mangrove Sediments Apriliani, Herlina; Pramono, Hendro; Oedjijono, Oedjijono; Widodo, Wimbuh Tri; Kristianto, Sonny; Putri, Rury Eryna; Unsunnidhal, Lalu
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.29245

Abstract

Oil spills resulting from shipping activities, tanker-based oil transportation, and fuel oil usage can cause coastal pollution, particularly in sensitive ecosystems such as mangroves. More than 90% of petroleum consists of hydrocarbons with complex carbon chain structures, making them difficult to decompose. Biological remediation using microorganisms offers a promising alternative for pollution mitigation, as microbes can degrade petroleum components and oxidizing hydrocarbons. This study aimed to evaluate the petroleum-degrading ability of selected bacterial isolates obtained from mangrove sediments at Logending Beach. The research employed experimental and survey methods. The primary parameter measured was Total Petroleum Hydrocarbons (TPH), while supporting parameters included pH and bacterial population density. The study consisted of several stages, including bacterial isolation, screening, and evaluation of the petroleum degradation capacity of selected isolates. The results identified two potential bacterial isolates capable of degrading crude oil. Isolate LG62 exhibited a degradation efficiency of 71.40%, while isolate LG105 showed a degradation efficiency of 57.10%. Petroleum concentrations of 2% (v/v) and 5% (v/v) were degraded more effectively than higher concentrations. Overall, the two bacterial isolates (LG62 and LG105) from Logending mangrove sediments demonstrated significant potential as bioremediation agents for petroleum hydrocarbon contamination.
Enhanced Survival of Lactobacillus Lg71 from Mangrove Sediment Under Simulated Gastric and Intestinal Conditions Kusharyati, Dyah Fitri; Satwika, Taruna Dwi; Alfisah, Rizal Khoirun; Mariana, Afifah; Rovik, Anwar; Oedjijono, Oedjijono
Journal of Biomedical Sciences and Health Vol. 3 No. 1 (2026)
Publisher : Universitas Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jbsh.v3.i1.291

Abstract

Background: For a probiotic to offer health benefits, it must stay viable through the tough environment of the human gastrointestinal tract. This study assesses the effectiveness of sodium alginate encapsulation in improving the survival of Lactobacillus LG71, a new strain from mangrove sediment, under simulated gastric and intestinal conditions. Objective: This study aims to evaluate the effectiveness of sodium alginate encapsulation in enhancing the viability and survival of Lactobacillus LG71, a probiotic strain isolated from mangrove sediment, during cold storage and under simulated gastric and intestinal conditions. Methods: Lactobacillus LG71 was encapsulated in sodium alginate beads. The viability of both encapsulated and free (non-encapsulated) cells was assessed over a 4-week storage period at 4 °C and during sequential exposure to simulated gastric and intestinal environments. Results: Encapsulation significantly improved survival rates compared to free cells (p < 0.05). Although both groups experienced a decline during the first week of storage, encapsulated cells maintained a high viability of approximately 107 CFU/mL, losing only 2.51 log CFU/g over four weeks. Most notably, encapsulated Lactobacillus LG71 demonstrated greater resilience during digestion, retaining populations of 2.21 log CFU/mL in gastric simulations and 1.00 log CFU/mL in intestinal simulations. In contrast, free cells were much more vulnerable to these acidic and enzymatic conditions. Conclusion: Sodium alginate encapsulation effectively protects Lactobacillus LG71 from environmental and biological stressors. These findings indicate that encapsulation is a crucial step for the commercial application of mangrove-derived probiotics, ensuring that a viable dose of living cells reaches the host's lower gastrointestinal tract to promote health benefits.
Co-Authors Alfisah, Rizal Khoirun Amalia, Dwi Ayu Lutfiani Andani, Ajeng Putri Retno anti, Monica Widi Apriliani, Herlina Ardhini R Maharning Aziz, Saefudin Daniel Joko Wahyono Dini Ryandini Dyah Fitri Kusharyati ELLY PROKLAMASININGSIH Endang Semiarti Fathurrohim, Ramadi Habib Hendro Pramono Isnasari, Azma Nurizqi JAKA WIDADA Kristianto, Sonny Mariana, Afifah nurainy, niharoh Nurmalasari, Ade Pratiwi, Meyta Ramadani, Adib Rony, Zahara Tussoleha Rovik, Anwar Rury Eryna Putri Satwika, Taruna Dwi Slamet Santoso Sodik Wuryanto, Sodik Sri Lestari Stalis Norma Ethica Tri Joko Raharjo Unsunnidhal, Lalu Widodo Widodo Wimbuh Tri Widodo