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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology BIO-SITE |BIOLOGI Sains Terapan Biosaintifika: Journal of Biology & Biology Education BioEksakta : Jurnal Ilmiah Biologi Unsoed Molekul: Jurnal Ilmiah Kimia
Oedjijono Oedjijono, Oedjijono
Universitas Jendral Soedirman
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Kemampuan Bakteri Halotoleran dari Sedimen Mangrove Pantai Logending dalam Pelarutan Fosfat dan Penambatan Nitrogen Ramadani, Adib; Oedjijono, Oedjijono; Pramono, Hendro; Pratiwi, Meyta
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 1 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.1.14854

Abstract

Lingkungan mangrove dapat menjadi sumber yang baik untuk mendapatkan bakteri halotoleran yang berpotensi sebagai agensia pupuk hayati dengan kemampuan melarutkan fosfat dan menambat N2 bebas. Tujuan penelitian ini adalah menguji toleransi isolat bakteri asal sedimen mangrove terhadap salinitas, menguji kemampuan isolat bakteri halotoleran dalam pelarutan fosfat dan penambatan nitrogen bebas, dan karakterisasi fenetik isolat bakteri halotoleran terpilih. Hasil penelitian menunjukkan bahwa dari 43 isolat yang diteliti, sebanyak 29 isolat toleran terhadap NaCl 5-7%. Dari 29 isolat, sebanyak 11 isolat bakteri halotoleran (>5% NaCl) diketahui mampu melarutkan fosfat dan menambat nitrogen dengan tiga isolat terpilih (LG36, LG60, dan LG66). Ketiga isolat tersebut menunjukkan efisiensi pelarutan fosfat masing-masing 125, 123,72, dan 126,92; dan kemampuan penambatan nitrogennya berturut-turut 38,02 ppm; 28,4 ppm; dan 51,93 ppm. Identitas ketiga isolat terpilih menunjukkan bahwa isolat LG60 merupakan spesies anggota genus Rhizobium, sedangkan isolat LG36 dan LG66 belum dapat diidentifikasi pada level genus. Kata kunci: fiksasi nitrogen, halotoleran, mangrove, pelarutan fosfat
Genotypic and Phenotypic Characterization of Alcaligenes javaensis JG3 Potential as an Effective Biodegrader Ethica, Stalis Norma; Oedjijono, Oedjijono; Semiarti, Endang; Widada, Jaka; Raharjo, Tri Joko
BIOTROPIA Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.448 KB) | DOI: 10.11598/btb.2018.25.1.583

Abstract

Utilization of glycerol by lipase producing bacteria offers great benefits for fat and oil waste degradation and waterwaste treatment. Nevertheless, there have been lack of reports about the availability of non-pathogenic, lipase producing bacteria, which could naturally degrade glycerol produced from the lipolysis process by lipase. This study reported a newly identified species of rhizobacteria, Alcaligenes javaensis JG3, which is not only able to produce high level of lipase, but also able to degrade glycerol molecules. Identification of strain JG3 was carried out using SEM (Scanning Electron Microscope), BD Phoenix 100 Automated Microbiology System and 16S rRNA gene analysis to determine its taxonomy status. The ability of the strain to metabolize glycerol was investigated both genotypically and phenotypically using degenerate PCR and a glycerol minimal medium. Identification test results showed that strain JG3 belongs to genus Alcaligenes, with the closest relationship with A. faecalis and A. aquatilis (96% nucleotide similarity maximum). Degenerate PCR resulted in a 248-bp sequence showing 93% similarity with glpK of Candidatus Sodalis pierantonius SOPE, a key gene involved in glycerol metabolism. In vitro glycerol utilization test result showed that Alcaligenes sp. JG3 was able to grow on glycerol aerobically, but not anaerobically. It is concluded that Alcaligenes sp. JG3 possesses genes coding for glycerol metabolism and this trait is phenotypically expressed, thus making the strain potential to be used as an effective fat and oil biodegrader.
Co-Authors Amalia, Dwi Ayu Lutfiani Andani, Ajeng Putri Retno anti, Monica Widi Ardhini R Maharning Aziz, Saefudin Daniel Joko Wahyono Dini Ryandini Dyah Fitri Kusharyati ELLY PROKLAMASININGSIH Endang Semiarti Fathurrohim, Ramadi Habib Hendro Pramono Isnasari, Azma Nurizqi JAKA WIDADA nurainy, niharoh Nurmalasari, Ade Pratiwi, Meyta Ramadani, Adib Rony, Zahara Tussoleha Slamet Santoso Sodik Wuryanto, Sodik Sri Lestari Stalis Norma Ethica Tri Joko Raharjo Widodo Widodo