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All Journal bionature Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) As-syifaa Jurnal Farmasi Jurnal Ilmiah Farmako Bahari Jurnal Mandala Pengabdian Masyarakat Kieraha Medical Journal Abdimas Indonesian Journal Journal Microbiology Science Jurnal Pengabdian Farmasi dan Sains MPSJ
Sitti Amirah
Fakultas Farmasi Universitas Muslim Indonesia
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Journal : Journal Microbiology Science

 1 
Molecular Identification of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.) Using Polymerase Chain Reaction (Pcr) Jamaluddin, Siti Nurlillah; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.994

Abstract

The roots of the bidara plant have the potential as antibacterial agents. The antibacterial compounds in the roots of the bidara plant originate from secondary metabolites produced by endophytic compounds known as endophytic fungi. The purpose of this research is to identify the types of endophytic fungi in bidara roots. The identification of microorganisms can be done through morphology or molecular methods. However, morphological identification alone is unable to depict the morphospecies to the phylogenetic level of a microorganism, thus molecular identification using the Polymerase Chain Reaction (PCR) method is required. This study utilized root isolates from the bidara plant with the sample code IFAZ-6, which, after sequencing, yielded a pair of base pairs measuring 558bp. The results of BLAST and phylogenetic analysis revealed that the IFAZ-6 isolate has a close relationship with the species Clonostachys rosea., which has been recorded in the database with a 100% identity level.
Potential of Endophyctic Fungi Isolates IFAZ-6 from Bidara Root (Ziziphus mauritiana Lam.) as Antibacterials Againts Digestive Tract Infection janna, Nur; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.995

Abstract

Bidara root (Ziziphus mauritiana Lam.) has activity as an antibacterial against digestive tract infections. This study aims to determine the antibacterial activity of isolate IFAZ-6 endophytic fungi of bidara root against bacteria that cause digestive tract infections using the TLC-Bioautography method. Purification was conducted on the active isolate IFAZ-6 to obtain a pure endophytic fungi isolate. Subsequently, macroscopic and microscopic observations were carried out. Then, fermentation was performed in MYB (Maltose Yeast Broth) medium for 21 days. Screening and evaporation were conducted to produce an extract. The obtained extract was subjected to TLC analysis using a chloroform: methanol eluent (7:1). The TLC-Bioautography test was conducted against Escherichia coli, Salmonella typhi, Shigella dysentriae, and Vibrio cholerae bacteria. The TLC identification results showed one spot with an Rf value of 0.80 for the IFAZ-6 isolate. The chromatogram profile obtained from the TLC-Bioautography method for the IFAZ-6 isolate demonstrated antibacterial activity against bacteria that cause digestive tract infections.
Antibacterial Activity of Endophytic Fungi Isolated from Bidara Roots (Ziziphus mauritiana Lam.) Against Bacteria That Cause Skin Infections Using TLC-Bioutography Sari, Andini Indah Permata; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.996

Abstract

One common disease found in tropical countries is skin infection. Inappropriate use of antibiotics to treat skin infections can lead to bacterial resistance, making it necessary to explore the source of bioactive compounds in natural materials. The Bidara plant (Ziziphus mauritiana Lam.) is known to have antibacterial properties. This research aimed to determine the antibacterial activity of endophytic fungal isolated from the parts of the Bidara plant against bacteria that cause skin infections using the TLC-Bioautography method. In this research, the isolate with the code IFAZ-6 was purified to obtain a pure endophytic fungal isolate, and macroscopic and microscopic examinations were conducted on that isolate. The obtained isolate was fermented for 21 days at room temperature using MYB (Maltose Yeast Broth) medium. The fermentation products were extracted using ethyl acetate (1:1). The obtained extract was further tested using TLC with a chloroform: methanol eluent (7:1). The TLC-Bioautography results showed Rf values of 0.81 and 0.58 indicating antibacterial activity against Staphylococcus aureus, Pseudomonas aeruginosa, Staphylococcus epidermidis, and Propionibacterium acnes bacteria. The identification of chemical components in the endophytic fungal isolate from the Bidara plant confirmed the presence of flavonoids, alkaloids, and anthraquinones. The extract from the endophytic fungal isolate with the code IFAZ-6 indicated antibacterial activity.
Antibacterial Activity of Endophytic Fungi from Bidara Roots Against Bacteria that Cause Skin Infections Nurdayani, Sitti; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.1001

Abstract

Endophytic fungi are fungi that grow and colonize plant tissues and are capable of producing bioactive compounds similar to those produced by their host. This study aims to determine the concentration of endophytic fungal isolates from Bidara roots (Ziziphus mauritiana Lam) that have antibacterial activity against bacteria that cause skin infections using the agar diffusion method. The research method begins with the rejuvenation of IFAZ-6 isolates, and microscopic and macroscopic observations are carried out. The isolates were fermented using Maltose Yeast Broth medium at 200 rpm for 21 days. The fermentate was extracted with an ethyl acetate solvent to obtain a thick extract. The tests carried out were minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibacterial activity tests carried out using the agar diffusion method. The results of the antibacterial activity test showed that the IFAZ-6 isolate extract at a concentration of 800 ppm showed antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Propionibacterium acnes. The statistical analysis results showed that the extract of isolate IFAZ-6 against Staphylococcus aureus and Staphylococcus epidermidis at concentrations of 800-1600 ppm and 1600–3200 ppm exhibited the same antibacterial activity. The bacterium Pseudomonas aeruginosa showed the same antibacterial activity at all tested concentrations, and the bacterium Propionibacterium acnes at concentrations of 1600–3200 ppm also exhibited the same antibacterial activity.
Optimization Time for Antibacterial Production of Endophytic Fungi Isolated from Bidara Root (Ziziphus mauritiana Lam.) Annisa, Khusnul; Fitriana, Fitriana; Amirah, Sitti
Journal Microbiology Science Vol 4, No 1 (2024)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v4i1.997

Abstract

Research on optimization time for antibacterial production of endophytic fungal isolated from bidara root (Ziziphus mauritiana Lam.) has been conducted, with the aim of determining the optimum production time for antibacterial compounds. Endophytic fungi are a group of fungi that live within plant tissues without causing harm to their host plants. In this research, the roots of bidara were isolated and purified using PDA media, isolates were followed by screening endophytic fungi isolates against test bacteria. The results of the endophytic fungal isolation from the bidara roots yielded 10 endophytic fungal isolates. The screening test against the test bacteria showed the largest inhibitory zone for IFAZ-6. Subsequently, the growth curve of these isolates was determined through fermentation using MYB media using a shaker speed of 200 rpm at a temperature of 25-28°C for 27 days. Then, mycelia and supernatant were collected on days 11, 13, 15, 17, 19, 21, 23, 25, and 27 with a 48-hour interval. The mycelia were weighed until a constant weight was obtained, while the supernatant was used to test the activity against the test bacteria using MHA media. The growth curve results indicated a stationary phase on day 21. The antibacterial activity test showed that Isolate IFAZ-6 reached optimum activity on day 27 with an inhibitory zone of 12.33 mm against Salmonella thypi.
Co-Authors A Hasrawati Adimi, Nurhikma Adriansyah, Muhammad Alani, Fitri Wasto Anak Agung Istri Sri Wiadnyani Andi Evi Wulandari Luspi Andi Maulana Kamri Andi Sulfika Annisa, Khusnul Arini Arini As’ad, Nur Asyirah Aulia Wati, Aulia Ayyub Harly Nurung Bayu Putra Bayu Putra Bayu Putra, Bayu Charawati, Nurul Mega Dita Pratiwi Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Franita Achmad Hasbullah, Nurhijjah Hendra Herman Heriyanto Beddu Hi Amin, Rahmatia S Irma Andriani Irma Santi Jamaluddin, Siti Nurlillah Janna, Nur Jihadi, Athira Kamri, Andi Khaedhir Mirawati Mirawati Mustakim, Putri Dwiyanti Adha NUR FADILAH Nurdayani, Sitti Nurung, Ayyub Harly Polopadang, Arion Whaizer R. Rusli Rachmat Kosman Rachmat Kosman, Rachmat Rahman, Safriana Rahmatia S Hi Amin Rakhmat, Syahgita Resvani, Syahyu Riany Novianti Y., Riany Rusli SAFITRI, INDAH DIAN Safriani Rahman Safriani Rahman Safriani Rahman Safriani Rahman Safriani Rahman Safriani Rahman, Safriani Sari, Andini Indah Permata Siska Nuryanti, Siska Triani Putri Ayu Zulkarnain MB, Iskandar