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Actinomycetes from the Soil of Chilli Plantation in Yogyakarta Showing an Antagonism to Fusarium oxysporum FU3 Audinah, Liya; Ilmi, Miftahul
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.5237

Abstract

 Application of biocontrol agent is an environmental-ly-friendly method to overcome the Fusarium wilt in chilli due to Fusarium oxysporum. This research aimed to isolate actinomycetes antagonist to F. oxysporum FU3 as biocontrol agent from the soil of chilli plantation in Kulon Progo, Yogyakarta. The actinomycetes were isolated using SCA medium and followed by screening for anti-F. oxysporum FU3 on PDA. The filtrate from isolates which showing the highest inhibition zone was produced and tested against strain FU3. The best isolate was then characterized. There are four iso-lates of actinomycetes showing antagonistic activity against F. ox-ysporum (AK4, AK5, AK7, and AK9). The highest antagonistic ac-tivity was shown by AK5 filtrate with inhibitions on PDA and PDB are 41.71 ± 3.5 % and 81.77 ± 5.1 %, respectively. Further obser-vation showed that filtrate of AK5 caused abnormality and lysis on FU3 hyphae. From the results, we concluded that AK5 is a poten-tial biocontrol agent against F. oxysporum. Further studies to opti-mize the production and formulize the bio-fungicide are suggested 
The Effect of Growth Temperature and pH on Lipid Production of Mucor irregularis JR 1.1 for Potential Biodiesel Applications Anggi Putri Pertiwi; Ilmi, Miftahul
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.26790

Abstract

Mucor irregularis JR 1.1 is a filamentous fungus that can produce lipids. The lipids produced by M. irregularis JR 1.1 reach 40-60%, so they can be used as raw material for biodiesel. Temperature and pH are important factors that support M. irregularis JR 1.1 lipid production. Optimum temperature and pH enhance the activity of enzymes involved in lipid production.  Based on previous research, the optimization of temperature and pH in the lipid production of M. irregularis JR 1.1 has not been determined. Therefore, this study aims to determine the optimal temperature and pH for lipid production by M. irregularis JR 1.1. The study involved varying temperature and pH treatments using Response Surface Methodology (RSM). Subsequently, lipid production was performed at the optimum temperature and pH under different incubation times. Based on the research results, the highest biomass production was observed at 35°C and pH 4, the highest lipid production was at 35°C and pH 5.5, and the highest glucose consumption rate was at a temperature of 23°C and pH 5.5. The highest lipid yield percentage was 6.2%, and the highest production rate was 0.011g/L/hour at the 96th hour of incubation. Statistical analysis showed high significance, with R² values of 95.88% for lipid production and 89.98% for biomass yield. These findings suggest that M. irregularis JR 1.1 has promising potential as a microbial lipid source for sustainable biodiesel production, particularly under optimized culture conditions.