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All Journal BALI MEDICAL JOURNAL (BMJ) CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Jurnal Kimia (Journal of Chemistry) ISM (Intisari Sains Medis) : Jurnal Kedokteran Bali Tourism Journal Journal of Global Pharma Technology Physical Therapy Journal of Indonesia

Ida Bagus Amertha Putra Manuaba

Departemen Pendidikan Kedokteran Dan Kesehatan, Fakultas Kedokteran, Universitas Udayana Denpasar, Indonesia

Author-ID : 588581

Religion Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Electrical & Electronics Engineering Health Professions Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Articles

Safety Procedure for Biosafety and Controlling a Communicable Disease: Streptococcus Suis Tandio, Deasy Ayuningtyas; Manuaba, Ida Bagus Amertha Putra
BALI MEDICAL JOURNAL Vol 5 No 2 (2016)
Publisher : BALI MEDICAL JOURNAL

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (355.911 KB)

Background: Streptococcus suis infection is a zoonotic disease which cause fatal outbreak. Infection case is related to animal handling and dry season. Health workers on Timor island need to understand more about biosafety procedure and increase awareness of the disease as a potential causes of meningitis. Objective: To provide a simple yet comprehensive reading material for the health workers that is exposed to meningitis. Method: This is a descriptive explorative study, to search about Streptococcus suis in the James Cook University OneSearch library search engine and biosafety procedure in WHO and CDC database. The information in accessed articles were compiled into a review piece. Conclusion: The biggest risk factor for a Streptococcus suis outbreak is inappropriate pig carcass handling. The cocci infect via micro-lesion on the handler skin. Public awareness about an appropriate way to handle meat needed to be raised. Suspected case need to be referred to the nearest centre with an ability to conduct a PCR test. It is essential that people, especially health workers understand that the principles of biosafety cover the basics of the containment system, including the practice, and the correct laboratory techniques, safety equipment, laboratory facilities to protect workers, the environment, and the public from exposure to infectious microorganisms.

HIDROLISIS BATANG JAGUNG SECARA ENZIMATIK DARI TANAH HUTAN MANGROVE Ni Nengah Kartini Asih; Putu Suarya; Ida Bagus Putra Manuaba; I Nengah Wirajana
CAKRA KIMIA (Indonesian E-Journal of Applied Chemistry) Vol 6 No 2 (2018): Volume 6, Nomor 2, 2018
Publisher : Magister Program of Applied Chemistry, Udayana University, Bali-INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.249 KB)

ABSTRAK: Tanah hutan mangrove merupakan salah satu sumber selulase. Aktivitas selulase pada tanah hutan mangrove pantai suwung kauh dengan substrat sekam padi telah dilakukan pada penelitian sebelumnya. Tujuan penelitian ini adalah untuk mengetahui waktu hidrolisis optimum dan aktivitas selulase dari tanah hutan mangrove dengan substrat batang jagung tanpa dan dengan delignifikasi. Tanah hutan mangrove Pantai Suwung Kauh Denpasar Bali digunakan secara langsung sebagai sumber selulase. Batang jagung tanpa dan dengan delignifikasi masing – masing dicampur dengan tanah hutan mangrove dan diinkubsi pada suhu 30oC dan pH 7,0 dengan variasi waktu inkubasi 0, 3, 5, 7, 9, dan 11 hari. Gula pereduksi hasil hidrolisis ditentukan dengan menggunakan metode Nelson – Somogyi yang absorbansinya diukur dengan spektrofotometer UV-Vis pada panjang gelombang 540 nm. Aktivitas selulase ditentukan berdasarkan penambahan produk gula pereduksi yang dihasilkan dalam rentang waktu inkubasi. Hasil penelitian menunjukkan bahwa waktu hidrolisis optimum pada sampel tanpa delignifikasi terjadi pada waktu inkubasi selama 5 hari, dengan konsentrasi gula pereduksi 4.5285 mg/mL. Sedangkan, waktu hidrolisis optimum pada sampel dengan delignifikasi terjadi pada waktu inkubasi selama 3 hari (B1), dengan konsentrasi gula pereduksi 16.2340 mg/mL. Aktivitas selulase tertinggi pada sampel tanpa delignifikasi dari hari ke-3 sampai ke-5 sebesar 2.6729 U/mL; dan pada sampel delignifikasi dari hari ke-1 sampai ke-3 sebesar 5.4328 U/mL. Hasil ini mengindikasikan bahwa tanah hutan mangrove memiliki aktivitas selulase untuk menghidrolisis substrat batang jagung dan proses delignifikasi berpengaruh terhadap aktivitas selulase. Kata kunci : batang jagung, delignifikasi, tanah hutan mangrove, selulase ABSTRACT: Mangrove forest soil is one of the sources of cellulase. Cellulase activity in mangrove forest soil from coast of Suwung Kauh Denpasar Bali with rice husk substrate has been carried out in previous studies. The purpose of this study is to determine the optimal hydrolysis time and cellulase activity of mangrove forest soil with corn stalks substrate with and without delignification. The mangrove forest soil from coast of Suwung Kauh Denpasar Bali was directly as a source of cellulase. The corn stalks with and without delignification were mixed with mangrove forest soil and incubated at 30oC and pH 7.0 with incubation times of 0, 3, 5, 7, 9 and 11 days, respectively. The reducing sugar of hydrolysis results was determined by using the Nelson-Somogyi method that the absorbances were measured by the spectrophotometer UV-Vis at wavelength 540 nm. The cellulase activities were determined based on the concentration of reducing sugar that resulted in the incubation period. The results showed that the optimum hydrolysis time in the sample without delignification occurred at an incubation time of 5 days, with a concentration of reducing sugar 4.5285 mg/mL. Whereas, the optimum hydrolysis time in the sample with delignification occurred at the incubation time for 3 days, and with a concentration of reducing sugar 16.2340 mg/mL. The highest cellulase activity in the sample without delignification was from days 3 to 5 of 2.6729 U / mL; and in the delignification sample was from days 1 to 3 of 5,4328 U / mL. The results of this study were that mangrove forest soil had the cellulase activity to hydrolyze substrate and delignification process had a effect on the cellulase activity.

PEMANFAATAN TEH KOMBUCHA SEBAGAI OBAT HIPERURISEMIA MELALUI PENGHAMBATAN AKTIFITAS XANTIN OKSIDASE PADA Rattus norvegicus Dewa Ayu Windu Manik Anandagiri; I. B. Putra Manuaba; Ni G. A. M. Dwi Adhi Suastuti
Jurnal Kimia (Journal of Chemistry) Vol. 8, No. 2 Juli 2014
Publisher : Program Studi Kimia, FMIPA, Universitas Udayana (Program of Study in Chemistry, Faculty of Mathematics and Natural Sciences, Udayana University), Bali, Indonesia

The formation of high levels of uric acid and problems of its excretion from the body can lead to hyperuricemia. This study serves to examine the kombucha tea as a hyperuricemia drug activity through the inhibition of the enzyme xanthine oxidase. Kombucha tea was used with three different variations of fermentation namely: tea A (4 days), tea B (8 days), tea C (12 days), and two variations of the dose, i.e. 10 mL/kg BW and 40 mL/kg BW. This study uses a “posttest only control group” design. A total of 27 Wistar rats (Rattus norvegicus) are grouped into 9 groups, K (negative control), H (control hyperuricemia), A (control allopurinol), P1 (tea A dose of 10 mL/kg BW), P2 (tea A dose 40 mL /kg BW), P3 (tea B dose 10 mL/kg BW), P4 (tea B dose 40mL/kg BW), P5 (tea C dose 10 mL/kg BW), P6 (tea C dose 40 mL/kg BW). The rats were given high feed purine for research, namely chicken liver juice and melinjo so that they contracted the hyperuricemia condition. The high purine feeds were administered on all groups of rats except the negative control group. Experiment was undertaken to compare the effectiveness of allopurinol with kombucha tea. This experiment was done for nine days. On the last day, an analysis of the levels of uric acid and xanthine oxidase activity was undertaken and the data was analyzed using ANOVA. The conclusion was that the biggest decline in drug for the treatment of hyperuricemia was kombucha tea B with the dose of 40 mL/kg BW.

Making Future of Bali: a leading destination for Medical Tourism Ida Bagus Amertha Putra Manuaba
Bali Tourism Journal Vol. 5 No. 3 (2021): (September-December 2021)
Publisher : Bali Tourism Board

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36675/btj.v5i3.66

Bali Provincial Government has taken several steps to diversify the Balinese economy sectors. Medical Tourism became a proposal in line with the Indonesian government’s tourism sector development plan for the past five years. They continue to improve the domestic health industry. Not wanting to depend on imports continuously, Authorities since 2020 has committed to making improvements in the governance of state-owned and private companies, especially in the pharmaceutical, medical devices, and health facilities. The construction of an International Hospital (RSI) in Sanur, Bali, in collaboration with the Mayo Clinic Cancer Hospital in the United States, has been seriously monitored to create leading health service standards in Indonesia. Later, the community can use Bali RSI to carry out various medical treatments. Therefore, in the future, health tourism can be properly established. Another image would be emerging: Bali, a top-class holiday destination that serves as a sanctuary to rejuvenate one’s health.

PROSEDUR PENGGUNAAN ALAT PERLINDUNGAN DIRI DAN BIOSAFETY LEVEL 1 DAN 2 Amertha Putra Manuaba
Intisari Sains Medis Vol. 6 No. 1 (2016): (Available online: 1 August 2016)
Publisher : DiscoverSys Inc.

Implementation of the Occupational Health and Safety is one efforts to create a workplace that is safe, healthy, and free from environmental pollution, to reduce occupational accidents and disease, which in turn can improve the efficiency and productivity of labor. This is a descriptive explorative study that discuss about personal protective equipment procedures and biosafety level 1 and 2. Biosafety level is a combination of practice and application of procedures by workers at the laboratory facilities and safety equipment usage when working with dangerous infectious pathogenic agents. Biosafety level term is also used to describe the safe method in handling and managing materials that can infect a laboratory. It can be concluded that biosafety is a discipline in handling and containment system against infectious microorganisms and hazardous biological materials. The principles of biosafety cover the basics of the containment system, including the practice, and the correct laboratory techniques, safety equipment, laboratory facilities to protect workers, the environment and the public from exposure to infectious microorganisms.

Potensi terapi kombinasi Liver Growth Factor (LGF) dan Adrenomedullin (ADM) sebagai harapan baru penatalaksanaan Azoospermia Non-Obstruktif (ANO): tinjauan pustaka Ni Made Dian Hartaningsih; I Putu Yuda Prabawa; Benediktus Bosman Ariesta Gusti Putu; Dwijo Anargha Sindhughosa; Ida Bagus Amertha Putra Manuaba; I Gusti Ngurah Pramesemara
Intisari Sains Medis Vol. 13 No. 1 (2022): (Available Online : 1 April 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/ism.v13i1.1363

Non-Obstructive Azoospermia (NOA) is caused by the failure of spermatogenesis process. This case becomes the highest prevalence, 95% of the total azoospermia. Therapeutic modalities in use today such as invasive techniques, hormonal therapy, and gene therapy are less effective in the treatment of azoospermia. In addition, the overall therapeutic modalities also have serious side effects such as infection, testicular atrophy, nerve damage and other side effects. Referring to the problems above, non-obstructive azoospermia is an urgent health issue and requires effective and efficient management with minimal side effects, as the combination of Liver Growth Factor (LGF) and Adrenomedullin (ADM). LGF is able to regenerate spermatogenesis after spermatogonia testicular stem cell damage. LGF also works specifically by stimulating germinal cells without changes in somatic cells. The motility of reactivation could be improved by combining the ADM into therapy, which bind to specific receptors, and the result of increasing the cAMP / PKA and NO that are important in the regulation of cilia’s movement. Combination LGF and ADM are potential to create new therapeutic candidates in the management of non-obstructive azoospermia, which can be immediately implemented as effective and efficient therapy. Azoospermia Non-Obstruktif (ANO) adalah azoospermia yang disebabkan kegagalan proses spermatogenesis dan merupakan kasus dengan prevalensi tertinggi (95% dari total azoospermia). Modalitas terapi yang digunakan sampai saat ini seperti teknik invasif, terapi hormonal, dan terapi gen terbukti belum efektif dalam tatalaksana azoospermia. Selain itu, keseluruhan modalitas terapi ini juga memiliki efek samping serius yang perlu diwaspadai seperti infeksi, testis atropi, kerusakan saraf, serta efek samping lainnya. Merujuk pada permasalahan diatas, azoospermia non-obstruktif merupakan masalah kesehatan yang mendesak dan membutuhkan tatalaksana yang efektif dan efisien dengan efek samping yang minimal seperti dengan kombinasi terapi LGF dan ADM. LGF mampu meregenerasi testis dan mereaktivasi spermatogenesis setelah kerusakan sel punca spermatogonia. LGF juga bekerja secara spesifik dengan menstimulasi sel-sel germinal tanpa menyebabkan perubahan pada sel-sel somatis. Motilitas sperma hasil reaktivasi spermatogenesis kemudian ditingkatkan dengan mengkombinasikan ADM kedalam terapi dimana berikatan pada reseptor-reseptor spesifik sehingga berefek pada peningkatan cAMP/PKA dan NO yang penting dalam pengaturan kibasan flagella. Perpaduan antara terapi LGF dan ADM ini sangat berpotensi menciptakan kandidat terapi baru dalam penatalaksanaan NOA yang efektif dan efisien.

Kemampuan Tutor dalam Menstimulasi Self Directed Learning Mahasiswa Kedokteran I Gusti Ayu Harry Sundariyati; Putu Gede Sudira; Ida Bagus Amertha Putra Manuaba; I Gusti Ayu Sri Darmayani
Intisari Sains Medis Vol. 13 No. 2 (2022): (In Press : 1 August 2022)
Publisher : DiscoverSys Inc.

Background: Self-directed learning (SDL) is a skill that must be possessed by medical students as a form of adult learning. To train these skills, students need tutors to stimulate students' SDL abilities. Methodology: This study was conducted quantitatively using a Dolman and Ginns questionnaire on third-year medical students. Results: Tutors in the Medical Emergency block were able to provide a good explanation of the issues in the case and stimulate students to look for other learning resources. Conclusion: Tutors with academic backgrounds that match the discussion material can be considered as one of the factors that can stimulate students' SDL. Latar Belakang: Self-directed learning (SDL) merupakan keterampilan yang harus dimiliki oleh mahasiswa kedokteran sebagai bentuk pembelajaran dewasa. Untuk melatih keterampilan ini mahasiswa memerlukan tutor dalam menstimulasi kemampuan SDL mahasiswa. Metodologi: penelitian ini dilakukan secara kuantitatif dengan menggunakan kuisioner Dolman dan Ginns pada mahasiswa kedokteran tahun ke-tiga. Hasil: tutor pada blok Medical Emergency mampu memberikan penjelasan yang baik mengenai issue yang ada pada kasus serta menstimulasi mahasiswa untuk mencari sumber pembelajaran yang lainnya. Simpulan: tutor dengan latar belakang akademik yang sesuai dengan materi diskusi dapat dipertimbangan sebagai salah satu factor yang dapat menstimulasi SDL mahasiswa.

Post-exercise hypotension phenomenon in elderly after aerobic exercise Agha Bhargah; Made Muliarta; I Putu Yuda Prabawa; Ida Bagus Amertha Putra Manuaba; Varennia Bhargah
Journal of Global Pharma Technology Volume 10 Issue 06: (2018) June 2018
Publisher : Journal of Global Pharma Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Post-exercise hypotension (PEH) which means the occurrence of changes in blood pressure after a physical exercise to a lower blood pressure level after the start of physical exercise. The incidence of PEH can be concluded as a benefit when viewed regarding people who experience a state of hypertension, but if in people with normal blood pressure circumstances can cause the risk of a state of hypotension. The purpose of this study was to determine the magnitude of the decrease in post-exercise hypotension in elderly. Study model using a quasi-experimental model with pre-test and post-test group design involving 28 elderly as a research subject. Aerobic exercise in this study was performed through a healthy heart gymnastics exercise with a duration of 45 minutes. Blood pressure measurements were performed before exercise (baseline value), immediately after exercise, 15 minutes, 30 minutes, 45 minutes, and 60 minutes after exercise was done. Statistical analysis using normality and homogeneity test, ANOVA test, and paired sample t-test to compare differences in blood pressure parameter between period of measurement. There are differences in systolic, diastolic, and Mean Arterial Pressure (MAP) blood pressure between time measurements (p<0.001). The systolic blood pressure was significantly lower at 60 minutes post exercise (121.96±1.43 mmHg) compared with baseline (141.35 ± 8.76) (p<0.001). Diastolic blood pressure was significantly lower at 60 minutes post exercise (121.96 ±1.43 mmHg) compared with baseline (91.75 ± 1.48) (p<0.001). MAP was significantly lower at 60 minutes post exercise (93.89 ± 1.15 mmHg) compared with baseline (108.39±1.34) (p<0.001). Aerobic exercise through a healthy heart gymnastics session on elderly provides a post-exercise hypotension phenomenon. The lowest reduction of blood pressure was found at 60 minutes after aerobic exercise.Keywords: Systolic, Blood Pressure, Hypotension, Diastolic.

Integrating simulation, role-play, and technology in physiotherapy education: A narrative review on teaching methods for patient education Manuaba, Ida Bagus Amertha Putra; Yani, Made Violin Weda; Indrakusuma, Anak Agung Bagus Putra; Dewi, Ketut Liana Arya; Wulandari, Putri Ayu; Mahadipaka K.A, Ngurah Arya T.; Sasmitha, Ni Luh Ayu; Supadmanaba, I Gede Putu
Physical Therapy Journal of Indonesia Vol. 7 No. 1 (2026): Inpress January-June 2026
Publisher : Universitas Udayana dan Diaspora Taipei Medical University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/ptji.v7i1.399

Physiotherapists play a crucial role in patient education, as effective communication and educational strategies directly influence treatment adherence, functional outcomes, patient satisfaction, and trust in healthcare services. The increasing global burden of musculoskeletal and neurological disorders has intensified the demand for physiotherapy services, emphasizing the need for graduates who are not only clinically competent but also proficient in patient-centered education. This narrative review aims to synthesize evidence from 2015 to 2025 regarding the integration of simulation, role-play, and educational technology in physiotherapy education, with a particular focus on teaching methods that enhance patient education competencies. Relevant peer-reviewed literature was identified from major academic databases, including PubMed, Scopus, and Google Scholar, and analyzed thematically to examine educational outcomes related to communication skills, learner engagement, confidence, empathy, and clinical preparedness. The findings indicate that simulation-based learning and structured role-play provide safe and experiential environments for students to practice patient communication and education, while technology-enhanced approaches such as virtual simulations and digital learning platforms offer scalable and flexible learning opportunities. Collectively, these methods demonstrate consistent benefits in improving students’ ability to deliver clear, structured, and empathetic patient education, thereby supporting readiness for clinical practice. Integrating simulation, role-play, and technology within physiotherapy curricula represents a strategic approach to strengthening patient education competencies and preparing graduates to deliver high-quality, patient-centered, and trusted physiotherapy care in response to contemporary healthcare demands.

Co-Authors Agha Bhargah Benediktus Bosman Ariesta Gusti Putu Deasy Ayuningtyas Tandio, Deasy Ayuningtyas Dewa Ayu Windu Manik Anandagiri Dewi, Ketut Liana Arya Dwijo Anargha Sindhughosa I G. P. Supadmanaba I Gusti Ayu Harry Sundariyati I Gusti Ayu Sri Darmayani I Gusti Ngurah Pramesemara I Nengah Wirajana I Putu Yuda Prabawa Indrakusuma, Anak Agung Bagus Putra Made Muliarta Mahadipaka K.A, Ngurah Arya T. Ni G. A. M. Dwi Adhi Suastuti Ni Made Dian Hartaningsih Ni Nengah Kartini Asih Putri Ayu Wulandari, Putri Ayu Putu Gede Sudira Putu Suarya Sasmitha, Ni Luh Ayu Varennia Bhargah Yani, Made Violin Weda

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