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All Journal Bioscience Biogenesis: Jurnal Ilmiah Biologi Jurnal Media Gizi Indonesia (MGI) Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology BIOEDUSCIENCE Jurnal Pengabdian Masyarakat AbdiMas Indonesian Journal of Biotechnology and Biodiversity Manuju : Malahayati Nursing Journal Poltekita : Jurnal Ilmu Kesehatan Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang Jurnal Pengabdian Nasional (JPN) Indonesia Jurnal Pengabdian kepada Masyarakat
Seprianto
Program Studi Bioteknologi, Fakultas Ilmu-ilmu Kesehatan, Universitas Esa Unggul, Jakarta, Indonesia 11510
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Journal : bioscience

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Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.901 KB) | DOI: 10.24036/0201822100216-0-00

Abstract

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.
Co-Authors Agustinus Robert Uria alfero Putra Iryanto Alipya Zhafira callista marsya Dewi Seswita Zilda Dijaya, Rendy Dionysius Subali Febriana Dwi Wahyuni feby feby Feliatra Gifari, Nazhif Hardinsyah Harna, Harna Himarwan, Aditya Lisa Rahma Dewi Muhammad Arreza Mury Kuswari nie nie Nora, Adri Novianti, Titta Nurmalasari, Mieke Nursanah Nursanah Oktaviani Naulita Turnip PUSPITA LISDIYANTI Putra, Felicia Kartawidjaja PUTRI HANDAYANI putri mega utami Rahmayanti R, Andi Rasya Aulia Rahmah Rian Adi Pamungkas Rimbawan , Rini Hidayati Rini Hidayati Roaslein Putri roaslein putri Ruhmayanti, Nur Ayu Sa'pang, Mertien Sabar Pambudi Saraswati, Henny Saraswati, Henny Suharsono Suharsono Tania Surgasari Titania Tjandrawati Nugroho Yusro Nuri Fawzya