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All Journal Jurnal Komunikasi Hukum Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Journal of Dentomaxillofacial Science Conservative Dentistry Journal Jurnal Media Akademik (JMA)

Ketut Suardita, Ketut

Faculty Of Dental Medicine, Universitas Airlangga

Author-ID : 596848

Religion Humanities Dentistry Economics, Econometrics & Finance Education Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Social Sciences Other

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Perbedaan daya pembersih kavitas saponin ekstrak kulit manggis (Garcinia mangostana Linn) 0,78% dan asam sitrat 6% (The difference of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity) Ivon Dewi Setianingrum; Ketut Suardita; Ari Subiyanto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries, and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cavity on the first group used 0,78% saponin from mangosteen pericarp extract as cavity cleanser, the second group used 6% citric acid as cavity cleanser, and the control group used aquadest. Then, the teeth were split to be observed on Scanning Electron Microscope (SEM). Result: For Mann- Whitney test there were significant differences just between 078% saponin from mangosteen pericarp extract with 6% citric acid, and 6% citric acid with aquadest, but not for 0,78% saponin from mangosteen pericarp extract with aquadest. Median value of 6% citric acid showed 2,000 which is the smallest value compared to the value of the other groups. Conclusion: The cleanliness of cavity with 6% citric acid is better than that with 0,78% saponin from mangosteen pericarp extract.

Effectiveness of flavonoid from mangosteen pericarp (Garcinia mangostana L.) as Enterococcus faecalis antibiofilm Dalhar Hakiki; Latief Mooduto; Ketut Suardita; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcinia mangostana pericarp against E. faecalis bacterial biofilm. Methods:Laboratory experimental in-vitro with post test only group design. The method used is microtitter plate biofilm assay and continued with the readings use Elisa reader at a wavelength of 595 nm. Results:Flavonoids mangosteen pericarp effective as antibiofilm E.faecalis bacteria at a concentration of 12.5%. Conclusion:The study showed that flavonoids from mangosteen pericarp has antibiofilm activity against E. faecalis bacterial biofilm.

EKSPRESI Interleukin-1 (IL-1) PADA PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis Yuliana Dwiwahyu Suryandari; Ketut Suardita; M. Mudjiono; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-1. IL-1 is the proinflammation cytokine that is the key of host response bacteria invation and tissue damage. Also IL-1 could cause some indirectly tissue damage through the activation of MMPS. MMPs to stop the collagen formation. Purpose. The aim of this study is to know about the expression of IL- 1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of IL-1 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of IL-1 is increasing during the periapical tissue damage that induced by E.faecalis.

EKSPRESI TGF-B DAN MMP-1 PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS TIKUS WISTAR Tamara Yuanita; Hadriany Hotmaria; Ruslan Effendy; Ketut Suardita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-ß to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-ß and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group A (negative control) : every tooth was’nt induced by anything. Group B ( positive control): every tooth was induced only by sterile BHIb and closed by GIC Fuji II as the final restoration. Group C (: every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), and closed by GIC Fuji II as the final restoration. The animals were sacrificed after 21 days and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-1 increased significantly in group C when E.faecalis was induced. When expression of TGF-ß decreaced significantly in group C rather than group B. Conclusion. From this study we know that the expression of TGF-ß and MMP-1 are make opposite pathway due to chronic apical periodontitis that induced by E.faecalis.

Perbedaan Perlekatan Biofilm Streptococcus mutans pada Resin Komposit Nanofil Tipe Universal Restortive dan Flowable Restorative Andi Kurniawan; Ketut Suardita; Nanik Zubaidah
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Back Ground: Adherence of Stretococcus mutans biofilm (S. mutans) to the surface of dental restorative materials is considered an important step in the development of secondary caries and periodontal disease. There are two type of nanofil composite: universal restorative and flowable restorative. That have different characteristic to induce S.mutans biofilm adherent in it surface. Purpose: The aim of this study was to compare the adherence of S. mutans biofilm to two types of nanofil restorative materials, flowable restorative and universal restorative.. Materials and Methode: 32 disc-shaped specimens (∅ = 5.0 mm / thickness = 2.0 mm) of two types composite were divided to 4 groups (n = 8): group 1; universal restorative were immersed in pH cycling solution for 14 days ,group 2; Universal restorative were immersed in water for 14 day,group 3: flowable restorative were immersed in pH cycling solution,group 4: flowable restorative were immersed for 14 days in water . in day 15 th, All speciments(n=32) were immersed for 24 hours in artificial saliva.. Streptococcus mutans cells were brought in contact with and grown on the speciments for 48 hours in BHI-B. Bacterial suspension was deposited onto each material and the adhesion of biofilm was evaluated trough optic density (OD) . Optic density biofilm of S.mutans analyzed using Elissa reader’ Spectrophotometry. Statistical analysis was performed by Kruskall -wallis and Tukey HSD test (α = 0.05). Result: Adherence of S.mutans biofilm on flowable restorative (mean OD:1,933, SD: 0,633) were significantly higher than universal restorative materials (mean OD: 1,240,SD:0,317). (P<0,05) Conclusion: The adherence of S.mutans biofilm on the surface of composites resin nanofil flowable restorative higher than universal restorative.

Daya Antibakteri Ekstrak Batang Pisang Ambon (Musa paradisiaca var. sapientum) terhadap Pertumbuhan Enterococcus faecalis H. Hafizha; Ketut Suardita; Nirawati Pribadi
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Background: Enterococcus faecalis is one of the most common bacteria in root canal after endodontic treatment. This species is found in about 77% of the cases that resistant to treatment. It is necessary to develop an alternative for intracanal dressing, one of the potential substance is natural ingredients. The stem of ambonese banana (Musa paradisiaca var. sapientum) extract contains active substances such as tannin, flavonoid, alkaloid, saponin, and antraquinone, which have been known for their antibacterial potency. Purpose: The aim of this study was to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of ambonese banana stem (Musa paradisiaca var. sapientum) extract against Enterococcus faecalis. Method: This research was a laboratory experimental study. Ambonese banana stem extract was made by maceration method with ethanol 96% and certain dilution was performed to obtain various concentration. Value of MIC and MBC of ambonese banana stem extract against Enterococcus faecalis were known by counting the growth of bacteria colonies on blood agar media in CFU/ml. Result: The percentages of bacteria colonies at concentration 85%; 82,5%; 80%; 77,5%; 75%; 72,5%; 70%; and 67,5% of ambonese banana stem extract in sequence were 0%; 0%; 0%; 0%; 0%; 0%; 7,44%; and 14,53% from the positive control. Conclusion: The stem of ambonese banana (Musa paradisiaca var. sapientum) extract had minimum inhibitory concentration (MIC) at 70% concentration and minimum bactericidal concentration (MBC) at 72,5% concentration against Enterococcus faecalis.

Perbedaan efektifitas penutupan tubulus dentin antara pasta gigi yang mengandung bioaktif glass (novamin) dan strontium chloride Profilia Shinta; Ketut Suardita; Moch. Mudjiono
Conservative Dentistry Journal Vol. 8 No. 2 (2018): July - December
Publisher : Universitas Airlangga

Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like strontium chloride and bioactive glass (Novamin). These agents cause occlusion of dentinal tubules thereby reducing hypersensitivity.Purpose: This study was to evaluate the effects of different desensitizing dentifrices on dentinal tubule occlusion by scanning electron microscopy (SEM) of strontium chloride and bioactive glass (Novamin) pastes in the treatment of dentinehypersensitivity (DH) Methods: sixteen extractedspecimens from bovine incisors teeth with randomized into 2 groups (n=8). The crowns were removed from the root and the crown were sectioned longitudinally into two parts (in a mesiodistaldirection). The cervical toothwere resulting in two samples per tooth.Dentinal tubules were exposedand thesamples provided a 4 mm x 4 mm area of exposed dentinal tubules.Group 1 (treated with strontium chloride), Group 2 (treated with bioactive glass (novamin))After each treatment for seven days, tubule occlusion on dentin were analyzed by scanning electron microscopy (SEM).The data were analyzed using “mann-whitneytest” (p<0.05).Result: Groups bioactive glass (novamin) showed tubule occlusion highest when compared with groups strontium chloride.Conclusion: bioactive glass (novamin) paste showed tubule occlusion highest with strontium chloride

Cytotoxicity test of 0,78% xanthone from mangosteen pericarp (Garcinia mangostana L.) and 0,2% chlorhexidine gluconate toward BHK-21 fibroblast cells Afin Aslihatul Ummah; Laksmiari Setyowati; Ketut Suardita
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.54-57

Background. Chlorhexidine gluconate is one of endodontic irrigants that has excellence capability to penetrate into dentin tubules and kill the pathogenic bacteria there. On the other hand, chlorhexidine gluconate has side effects to cause allergic reactions of the tissue and discoloration of the teeth. Xanthone from mangosteen pericarp can be considered as a natural alternative irrigant that usually has a good tolerance to the body. Purpose. The aim of this study compared the cytotoxicity between 0,78% xanthone from mangosteen pericarp and 0,2% chlorhexidine gluconate toward BHK-21 fibroblast cells. Methods. This study used experimental post-test only control group design. Xanthone from mangosteen pericarp preliminary cytotoxicity tested in various concentrations. Xanthone from mangosteen pericarp classified as a non-toxic concentration at 0,78%. Cytotoxicity of 0,78% xanthone from mangosteen pericarp compare with cytotoxicity of 0,2% chlorhexidine gluconate using MTT assay method. Cytotoxicity of material can be seen from % of cell viability. Viable cell measured by the result of optical density that read by ELISA reader 620 nm. Result. 0,78% xanthone from mangosteen pericarp showed lower cytotoxicity than 0,2% chlorhexidine gluconate toward BHK-21fibroblast cells. One-way ANOVA showed a significant difference between the study groups (P<0,05). Conclusion. 0,78% xanthone from mangosten pericarp showed lower cytotoxicity than 0,2% chlorhexidine gluconate toward BHK-21 fibroblast cells.

A review of tissue engineering in regenerative endodontic treatment Prasetyo, Eric Priyo; Wahjuningrum, Dian Agustin; Sampoerno, Galih; Sukandar, Wilson; Shariz Bin Sharizal, Shafy; Paidal, Nurfahira; Fadiyan Amriel, Menza; Gunawan, Nathania Elita; Suardita, Ketut; Tjendronegoro, Evelyn
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.1-5

Background: Regenerative endodontic treatments are classified based on approach in the procedures and principles. One is cell-free approach that involves a revitalization procedure to achieve continued root growth while the other is cell-based approach which involves pulp/dentin regeneration through isolation and ex vivo expansion of stem cells seeded in the scaffold and then transplanted into the canal space. Technically, the cell-free approach is simpler than cell-based approach because the former does not concern about external stem cell isolation and culture. Currently, the gold standard and the one that has been clinically performed is cell-free approach. Cell-based approach is still not a standard because currently the cell-based approach is still in the clinical trial stage. Purpose: to evaluate the biological basis and clinical protocols recently used in regenerative endodontic treatment and discuss potentially future treatment approaches. Review: The literature review was searched in PUBMED with the keywords: immature permanent teeth, pulp revascularization, stem cells and regenerative endodontics. The regenerative endodontic treatments with various procedures and techniques result in a significant increase in root length and dentinal wall thickness. Stimulation of stem cells in apical root canal system is required to induce tissue formation and continued root maturation. Conclusion: The success of regenerative endodontic therapy relies on the development of a technique that enables clinicians to create functional pulp tissue within cleaned and shaped root canal systems. It is hoped that further research studies are required to define advantages and limitations of cell-free and cell-based approach.

Differences of compressive strength between calcium carbonate from blood clam shells and calcium hydroxide as a candidate for pulp capping material Saraswati, Widya; Juniarti, Devi Eka; Lestari, Vita; Soetojo, Adioro; Kunarti, Sri; Cahyani, Febriastuti; Suardita, Ketut; Rahardia, Nabiela
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.11-14

Background: Pulp capping is one of the treatments for reversible pulpitis and aims to maintain pulp vitality. This treatment requires a material that can protect the pulp with good biocompatibility. The physical and mechanical properties, bio interactivity and bioactivity of pulp capping materials are very important for the formation of reparative dentin. Calcium hydroxide (Ca(OH)2) as the gold standard material in pulp capping treatment also has some disadvantages. Another alternative for pulp capping material is blood clam shell because it contains 98% calcium carbonate (CaCO3), which is a compound with a bone-like structure and can induce pulp cell differentiation. Objective: To investigate and explain the difference in compressive strength between CaCO3 from blood clam shells and Ca(OH)2 as a candidate pulp capping material. Methods: This research is a laboratory experimental study with post test only control group design method. Ca(OH)2 and CaCO3 samples were formed with a mixture of powder and aquadest with 4x6 mm sample size. The samples were dried at room temperature and the compressive strength was measured using a universal testing machine (UTM). Result: There is a significant difference in compressive strength between Ca(OH)2 and CaCO3 blood clam shells in the Mann-Whitney test results (p<0.05). Conclusion: The results of the compressive strength test between the mixture of Ca(OH)2 with aquadest in a ratio of 1:1 are greater than the mixture of CaCO3 blood clam shells with aquadest in a ratio of 3:1 so that pure CaCO3 blood clam shells with distilled water without other additives cannot be used as a candidate for capping pulp material.

Co-Authors Adioro Soetojo Afin Aslihatul Ummah Agustin Wahjuningrum, Dian Amy Nindia Carabelly Andi Kurniawan Ari Subiyanto Bin Zainal Abidin, Imran Dalhar Hakiki Daniyal Lazuardi Ramadhan Derice Putri Nourah Serena Devi Eka Juniarti Devi Puspitasari Dian Agustin Wahjuningrum Elvira Theola Judith Eric Priyo Prasetyo Fadiyan Amriel, Menza Fani Pangabdian Febriastuti Cahyani Febriastuti Cahyani Galih Sampoerno Gunawan, Nathania Elita H. Hafizha Hadriany Hotmaria Harry Huiz Peeters I Putu Andika Pratama Ira Arundina Ira Widjiastuti Ivon Dewi Setianingrum Kharisna, Deaniddo Laksmiari Setyowati Latief Mooduto, Latief Lestari, Vita M. Mudjiono Moch. Mudjiono Nabila, Rizka Affan Nanik Zubaidah Natan, Daniel Nirawati Pribadi Ohara, Masaru Paidal, Nurfahira Priyo Prasetyo, Eric Profilia Shinta Radixtio Auzan Fepiosandi Rahardia, Nabiela Ruslan Effendy Setyabudi Shariz Bin Sharizal, Shafy Slamet Soetanto Sri Kunarti, Sri Suhartono Taat Putra Sukandar, Wilson Syahria, Hania Dana Tamara Yuanita Tiara Dyah Iswari Tjendronegoro, Evelyn Victoria Beatrice Angelica Widya Saraswati Wijanarko, Christina Immee Wijaya, Kadek Dwitya Partha Yuliana Dwiwahyu Suryandari Zora, Zofia

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