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All Journal Majalah Obstetri dan Ginekologi Biomedical Journal of Indonesia Majalah Kesehatan FKUB Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Ilmu Kedokteran dan Kesehatan Journal of Issues in Midwifery Sciences of Pharmacy Journal of Food and Pharmaceutical Sciences Jurnal Info Kesehatan
Subandi Subandi
Fakultas Kedokteran Universitas Brawijaya
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Effect of Curcumin Nanoemulsion on Brain Cell Development, Locomotor Function, and Mortality of Zebrafish Larvae Model of Gestational Diabetes Mellitus Subandi, Subandi; Azahra, Mutiara Syifa; Ovinery, Amarsya; Akhmalia, Qorry' Afyu; Asih, Aliyah Tresna; Sulistomo, Hikmawan Wahyu; Nurdiana, Nurdiana; Wiyasa, I Wayan Arsana
Biomedical Journal of Indonesia Vol. 11 No. 3 (2025): Vol 11, No 3, 2025
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v11i3.270

Abstract

Introduction. Gestational diabetes mellitus (GDM) is glucose intolerance in pregnancy due to reduced ability of pancreatic beta cells to produce insulin, causing oxidative stress that triggers various complications such as brain apoptosis to locomotor disorders and decreased head size which has an impact on mortality rates. Pharmacological treatment of GDM has side effects that risk affecting fetal development. Therefore, curcumin as a herbal medicine can be an option for GDM treatment because it can increase insulin sensitivity by activating the peroxisome proliferator-activated receptor-gamma (PPAR-gamma) receptor. Methods. Post Test Only Control Group Design was used in this experimental study which was tested on each research variable consisting of 25 zebrafish embryos divided into 5 treatment groups consisting of a negative control, a positive control exposed to 3% glucose, and a diabetic group given curcumin nanoemulsion with three different doses (0.3125 μg/ml, 0.625 μg/ml, 1.25 μg/ml). One Way ANOVA and Post Hoc Tukey tests were used to analyze the data from this study. Results. P value from One Way ANOVA test p=0.024 for brain apoptosis, p=0.00 for locomotor ability, p=0.04 for head size, and p=0.006 for mortality rate. Turkey Post Hoc test showed significant differences in the control and 3% glucose + 0.625 µg/ml curcumin nanoemulsion groups (p<0.05). Conclusion. Curcumin nanoemulsion therapy has an effect on decreasing brain apoptosis, increasing locomotor, inhibiting the decrease in head size, and decreasing the mortality rate in zebrafish with gestational diabetes. The most effective dose of curcumin nanoemulsion is 0.625 µg/ml.
Nanoemulsion of Curcuma longa Extract Improves the Developmental Process in High Glucose-Induced Zebrafish (Danio rerio) Embryos Arista, Devi Maya; Amelia, Rizki; Khotimah, Husnul; Subandi, Subandi; Priyahita, Fatnuraini; Fathir, Nabila; Subakti, Nastyazka; Syarafina, Hafizhah Dini
JURNAL INFO KESEHATAN Vol 23 No 3 (2025): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol23.Iss3.1889

Abstract

Gestational diabetes mellitus (GDM) is hyperglycemia during pregnancy, which can increase oxidative stress and reactive oxygen species (ROS). This oxidative stress can adversely affect fetal developmental processes, including body length, ocular development, blood flow, and heart rate. Curcuma longa extract, containing curcumin, has been shown to possess antidiabetic and antioxidant properties. Nanoemulsions have demonstrated efficacy in enhancing the pharmacokinetics and pharmacodynamics of herbal compounds. This study aimed to evaluate the effect of a nanoemulsion of Curcuma longa extract on body length, ocular apoptosis, blood flow, and heart rate in zebrafish (Danio rerio) embryos exposed to high glucose concentrations. Zebrafish has many benefits as it has 70% orthologous genes with humans, high fecundity, and rapid embryogenesis. Nanoemulsions were prepared using the self-nanoemulsifying drug delivery system (SNEDDS) technique. Embryos were allocated to five groups: control, 3% glucose, and 3% glucose with 1.25, 2.5, or 5 µg/mL nanoemulsion of Curcuma longa extract. The results showed that a 1.25 µg/mL nanoemulsion of Curcuma longa extract reduced phosphoenolpyruvate carboxykinase (PEPCK) expression, increased body length, and mitigated developmental process disorders by decreasing ocular apoptosis and maintaining blood flow and heart rate in high glucose-induced zebrafish embryos. These findings suggest that a nanoemulsion of Curcuma longa extract may have potential therapeutic applications in managing the adverse effects of hyperglycemia on fetal development in GDM.
The Effects of Black Garlic on Ovarian Malondialdehyde, Oviduct Muscle, and Endometrial Arterioles in Smoke-Exposed Rats Anandasari, Noni Fidya Ayu; Kridayanti, Novalia; Kaspirayanthi, Ni Ketut Devy; Nurseta, Tatit; Raras, Tri Yudani Mardining; Khotimah, Husnul; Reksohusodo, Subandi; Anita, Kenty Wantri; Yudhanto, Hendy Setyo
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1533

Abstract

The effect of cigarette smoke exposure on reproductive health has been widely studied, showing various physiological disorders induced by free radicals and oxidative stress. This study aimed to assess the protective effect of ethanol extract of black garlic (Allium sativum) on some reproductive parameters of female rats Rattus norvegicus exposed to subacute cigarette smoke. A proper experimental method with a post-test-only control group design was used, involving 25 rats divided into five groups with three different doses of the extract. The rats were exposed to cigarette smoke and then treated for 28 days. Results showed a significant decrease in ovarian Malondialdehyde levels and increased fallopian tube smooth muscle thickness in the extract-treated group. In addition, black garlic extract successfully increased the number of endometrial arterioles in the group exposed to cigarette smoke and extract, in contrast to the group exposed to cigarette smoke only. This study concludes that black garlic extract has the potential to be a protective agent against oxidative damage in the reproductive system of rats exposed to cigarette smoke, with effectiveness that depends on the dose of extract given. Further studies should investigate the long-term effects and optimal dosing of black garlic extract in humans. Additionally, exploring the molecular mechanisms behind its protective properties could enhance its application in reproductive health management.                 
Analysis of the Effect of Black Garlic (Allium sativum) Extract on Ovarian Follicular Atresia, Endometrial VEGF Expression, and Fallopian Tube Epithelial Cell Count in Rats (Rattus norvegicus) Exposed to Cigarette Smoke Kridayanti, Novalia; Anandasari , Noni Fidya Ayu; Kaspirayanthi, Ni Ketut Devy; Nurseta, Tatit; Raras, Tri Yudani Mardining; Khotimah, Husnul; Reksohusodo, Subandi; Angelica, Aina; Anita, Kenty Wantri; Yudhanto, Hendy Setyo
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1535

Abstract

Cigarette smoke exposure significantly impairs reproductive function in Rattus norvegicus. This study evaluated the protective effects of black garlic extract against such damage. Using a post-test-only control group design, 25 female Wistar rats were divided into five groups and exposed to cigarette smoke with or without varying doses of black garlic extract. After four weeks, results indicated that black garlic extract significantly increased Vascular Endothelial Growth Factor expression, enhanced fallopian tube secretory epithelial cell counts, and reduced ovarian follicular atresia in rats exposed to cigarette smoke. The group receiving 50 mg/kgBW of black garlic extract showed the most significant improvements. Statistical analysis, including One-way ANOVA, revealed significant differences between groups. Normality was assessed using the Shapiro-Wilk test, and homogeneity was confirmed with the Levene test. Significant decreases in ovarian follicular atresia (p < 0.05) and increases in Vascular Endothelial Growth Factor expression (p < 0.05) and secretory epithelial cell counts (p < 0.05) were observed in the 50 mg/kgBW treatment group compared to controls. In conclusion, black garlic extract offers dose-dependent protection against cigarette smoke-induced reproductive damage, with 50 mg/kgBW being the optimal dose. Further research should explore molecular mechanisms, long-term toxicity, and clinical applications in humans.              
Evaluation of Hematotoxicity in Female Wistar Rats Following Sub-Acute Inhalation Exposure to Polyethylene Microplastic Sulistomo, Hikmawan Wahyu; Setyowati, Anisa; Situmorang, Melani Chysti; Sulistiani, Ita; Wardani, Dewi Azar Nuria; Gusti, Kharisma Ciptaning; Nurdiana, Nurdiana; Kusuma, Ihda Dian; Rahardjo, Bambang; Reksohusodo, Subandi
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1585

Abstract

Polyethylene (PE) becomes a source of microplastics that can be widely distributed through the digestive and respiratory systems. However, its effects on blood cells are still being investigated. This study aims to analyze the impact of Polyethylene Microplastic (PE-MPs) exposure on the blood of female rats, including erythrocytes, leukocytes, and platelets. This study used female Wistar rats, which were divided into control and PE-MP groups. PE-MP was administered via whole-body inhalation at a concentration of 15 mg/m³ for 4 hours daily for 28 days. The absorption of plastic particles detected in the human bloodstream is likely to occur through mucosal contact (either through ingestion or inhalation). After the exposure period, the rats were euthanized to collect blood samples through the heart. A complete blood count was performed using an automatic hematology analyzer, and blood morphology was analyzed using thin blood smears. This study used the Mann-Whitney test. PE-MP exposure increased erythrocyte and platelet counts without a corresponding rise in leukocytes. Erythrocytes showed abnormal morphology (12.73% with ovalocytes and tear-shaped cells). Erythrocyte indices (MCV, MCH, MCHC) showed no significant differences. Platelet count rose by 1.7% (p-value= 0.017). Leukocyte and neutrophil counts were lower (0.84 and 0.94 times lower, respectively), while lymphocytes and monocytes were higher (1.03 and 1.61 times higher, respectively) in the PE-MP group compared to controls. The neutrophil-to-lymphocyte ratio did not differ significantly. PE-MP exposure in rats disrupts blood parameters, altering erythrocyte morphology and increasing platelet counts. Potential causes include oxidative stress, immune responses, and compensatory mechanisms. Study limitations include a small sample size and exclusive focus on inhalation exposure. Integrating multiple exposure routes (inhalation, ingestion, dermal) could offer a broader view of microplastic impacts. Future research with larger samples, diverse doses and durations, and exploration of additional markers or organ-specific effects is crucial for understanding PE-MP toxicity in real-world scenarios.               
Effect of Curcumin Nanoemulsion on Insulin Levels and Malondialdehyde Concentration in Zebrafish Embryos Model of Gestational Diabetes Mellitus Subandi, Subandi; Paramita, Galuh Anggarda; Khoir, Safa’atul; Nurdiana, Nurdiana; Wiyasa, I Wayan Arsana
Jurnal Ilmu Kedokteran dan Kesehatan Vol 12, No 11 (2025): Volume 12 Nomor 11
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v12i11.21464

Abstract

Gestational Diabetes (GD) is a condition characterized by increased blood sugar levels (hyperglycemia) in pregnant women who were previously undiagnosed with diabetes. Hyperglycemia disrupts the performance of pancreatic β cells that are responsible for producing the hormone insulin. Curcumin functions as an anti-inflammatory, allowing the insulin reaction to occur more quickly and insulin production to increase. Methods. Post Test Only Control Group Design was used in this experimental study which was tested on each research variable consisting of 1000 zebrafish embryos divided into 5 treatment groups with each sample containing 40 embryos, consisting of a negative control, a positive control exposed to 3% glucose, and a diabetic group given curcumin nanoemulsion with three different doses (0.3125 μg/ml, 0.625 μg/ml, 1.25 μg/ml). The exposure was carried out at 2-50 hpf and then measured using INS ELISA Kit and MDA ELISA Kit. The results were analyzed using a one-way ANOVA test. Results. NOVA showed p = 0.254 for insulin levels (not significant) and p = 0.029 for MDA concentrations. Post-hoc analysis revealed that the significant difference occurred only between the negative (K−) and positive (K+) control groups (p = 0.012), while the curcumin-treated groups (K1, K2, K3) showed no statistically significant differences compared with either control group (p > 0.05). Conclusion. Curcumin nanoemulsion tended to increase insulin levels and reduce MDA concentrations, but these effects were not statistically significant (p > 0.05). The induction of hyperglycemia with 3% glucose successfully established oxidative stress in zebrafish embryos, validating the model. Further studies with larger sample sizes and molecular analyses are needed to confirm the biological trends observed.
Co-Authors AA Sudharmawan, AA Abd. Rasyid Syamsuri Aina Angelica Akhmalia, Qorry' Afyu Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Andriani, Lela Dwi Angelica, Aina Anisa Setyowati Aris Widayati Arista, Devi Maya Asih, Aliyah Tresna Astri Proborini Azahra, Mutiara Syifa Balindra, Fredlina Rossa Bambang Rahardjo Chatarina Umbul Wahyuni Daniel Happy Putra Dewi Ariani Dewi Azar Nuria Wardani Dian Hanifarizani, Rahma Fathir, Nabila Gizta, Aura Bella Gusti, Kharisma Ciptaning Hendy Setyo Yudhanto Hikmawan Wahyu Sulistomo Husnul Khotimah Husnul Khotimah Ihda Dian Kusuma Ihda Dian Kusuma Ita Sulistiani Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita Kenty Wantri Anita, Kenty Wantri Kharisma Ciptaning Gusti Khoir, Safa’atul Kridayanti, Novalia Kusworini Handono Maharani, Wening Pangesthi Melani Chysti Situmorang Nasution, Annio Indah Lestari Ni Ketut Devy Kaspirayanthi Noni Fidya Ayu Anandasari Novalia Kridayanti Nurdiana Nurdiana Nurdiana Nurdiana Nurdiana Ovinery, Amarsya Paramita, Galuh Anggarda Priyahita, Fatnuraini Rismaina Putri Rizki Amelia Safitri, Risma Andani Ayu Sahara, Aulia Ilma Savitri, Faradea Ubaidurrohmah Setyowati, Anisa Sigit Purbadi Silvani, Yulia Sisca Dewi Karlina, Sisca Dewi Situmorang, Melani Chysti Subakti, Nastyazka Sulistiani, Ita Sulistomo, Hikmawan Wahyu Syarafina, Hafizhah Dini Tatit Nurseta Tatit Nurseta Tri Yudani Mardining Raras Tri Yudani MR Wardani, Dewi Azar Nuria Wati, Linda Ratna Wiyasa, I Wayan Arsana Yudhanto, Hendy Setyo