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All Journal Majalah Obstetri dan Ginekologi Biomedical Journal of Indonesia Majalah Kesehatan FKUB Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Ilmu Kedokteran dan Kesehatan Journal of Issues in Midwifery Majalah Kesehatan Indonesia Journal of Food and Pharmaceutical Sciences Jurnal Info Kesehatan
Subandi Subandi
Fakultas Kedokteran Universitas Brawijaya
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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PENGARUH NANO KURKUMIN TERHADAP TEKANAN DARAH PADA MODEL TIKUS PREEKLAMSIA Subandi; Syamsiatul Akhyar Riyadz; Nurdiana
Majalah Kesehatan Vol. 12 No. 4 (2025): Majalah Kesehatan
Publisher : Faculty of Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/majalahkesehatan.2025.012.04.1

Abstract

Preeklamsia adalah kelainan hipertensi serius pada ibu hamil yang menjadi kontributor terbesar jumlah kematian ibu di Indonesia pada tahun 2023. Munculnya hipertensi pada preeklamsia berhubungan dengan gangguan fungsi endotel, berupa penurunan bioviabilitas nitric oxide akibat stres oksidatif. Nano kurkumin sebagai antioksidan dapat mengurangi stres oksidatif dan memperbaiki fungsi endotel, tetapi efektivitasnya dalam memengaruhi tekanan darah pada preeklamsia perlu ditinjau lebih lanjut. Penelitian ini bertujuan untuk mengetahui pengaruh nano kurkumin terhadap tekanan darah pada model tikus preeklamsia (dengan pemberian L-NAME). Jenis penelitian eksperimental murni digunakan melalui pendekatan post test only control group design. Sampel yang diambil sebanyak 24 tikus bunting, terbagi ke dalam kelompok kontrol negatif dan positif, serta 4 kelompok perlakuan (P1, P2, P3, dan P4) dengan pemberian 4 dosis nano kurkumin berbeda (25 mg/KgBB, 50 mg/KgBB, 100 mg/KgBB, dan 200 mg/KgBB). L-NAME dan nano kurukumin diberikan mulai usia kehamilan hari ke-13 hingga ke-18. Data tekanan darah diambil saat usia kehamilan hari ke-19. Hasil penelitian ini menunjukkan bahwa nano kurkumin berpengaruh terhadap tekanan darah pada model tikus preeklamsia (one-way ANOVA, p = 0,000 < 0,05). Hasil uji post hoc LSD menunjukkan bahwa nano kurkumin 25 mg/KgBB (P1) secara signifikan mencegah peningkatan tekanan darah dibandingkan kelompok preeklamsia tanpa nano kurkumin (K+), yaitu pada variabel tekanan darah sistolik (p = 0,001) dan tekanan darah diastolik (p = 0,000). Penelitian menyimpulkan bahwa nano kurkumin dapat mencegah peningkatan tekanan darah pada preeklamsia. Nano kurkumin 25 mg/KgBB adalah dosis paling efektif.
Nanoemulsion of Curcuma longa Extract Improves the Developmental Process in High Glucose-Induced Zebrafish (Danio rerio) Embryos Arista, Devi Maya; Amelia, Rizki; Khotimah, Husnul; Subandi, Subandi; Priyahita, Fatnuraini; Fathir, Nabila; Subakti, Nastyazka; Syarafina, Hafizhah Dini
JURNAL INFO KESEHATAN Vol 23 No 3 (2025): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol23.Iss3.1889

Abstract

Gestational diabetes mellitus (GDM) is hyperglycemia during pregnancy, which can increase oxidative stress and reactive oxygen species (ROS). This oxidative stress can adversely affect fetal developmental processes, including body length, ocular development, blood flow, and heart rate. Curcuma longa extract, containing curcumin, has been shown to possess antidiabetic and antioxidant properties. Nanoemulsions have demonstrated efficacy in enhancing the pharmacokinetics and pharmacodynamics of herbal compounds. This study aimed to evaluate the effect of a nanoemulsion of Curcuma longa extract on body length, ocular apoptosis, blood flow, and heart rate in zebrafish (Danio rerio) embryos exposed to high glucose concentrations. Zebrafish has many benefits as it has 70% orthologous genes with humans, high fecundity, and rapid embryogenesis. Nanoemulsions were prepared using the self-nanoemulsifying drug delivery system (SNEDDS) technique. Embryos were allocated to five groups: control, 3% glucose, and 3% glucose with 1.25, 2.5, or 5 µg/mL nanoemulsion of Curcuma longa extract. The results showed that a 1.25 µg/mL nanoemulsion of Curcuma longa extract reduced phosphoenolpyruvate carboxykinase (PEPCK) expression, increased body length, and mitigated developmental process disorders by decreasing ocular apoptosis and maintaining blood flow and heart rate in high glucose-induced zebrafish embryos. These findings suggest that a nanoemulsion of Curcuma longa extract may have potential therapeutic applications in managing the adverse effects of hyperglycemia on fetal development in GDM.
The Effect of Nanocurcumin Administration on Plasma Malondialdehyde (MDA) Levels in Pregnant Wistar Rats (Rattus norvegicus) as a Preeclampsia Model: Pengaruh Pemberian Nano Kurkumin terhadap Kadar Malondialdehyde (MDA) Plasma Darah Pada Tikus Wistar (Rattus norvegicus) Bunting Model Preeklampsia Subandi, Subandi; Sari, Laras Indah; Nurdiana, Nurdiana
Majalah Kesehatan Indonesia Vol. 6 No. 1: 2025
Publisher : Utan Kayu Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47679/makein.2025226

Abstract

Preeclampsia is a pregnancy complication associated with oxidative stress and increased levels of malondialdehyde (MDA), leading to endothelial dysfunction and impaired placental perfusion. Nanocurcumin possesses antioxidant properties that can mitigate oxidative stress; however, its effectiveness in reducing MDA levels in preeclampsia requires further investigation. This study aims to evaluate the effect of nanocurcumin administration on plasma MDA levels in pregnant Rattus norvegicus models of preeclampsia induced using Nw-Nitro-L-Arginine Methyl Ester (L-NAME). A total of 24 pregnant Wistar rats were divided into six groups: negative control (K-), positive control (K+), and four treatment groups receiving nanocurcumin at doses of 25 mg/KgBW (P1), 50 mg/KgBW (P2), 100 mg/KgBW (P3), and 200 mg/KgBW (P4). Preeclampsia was induced by administering L-NAME (125 mg/KgBW) for six consecutive days, followed by nanocurcumin treatment. Plasma MDA levels were measured using the Thiobarbituric Acid (TBA) assay and analyzed using the Kruskal-Wallis test and post-hoc Mann-Whitney test (p < 0.05). Nanocurcumin administration at 25 mg/KgBW and 50 mg/KgBW significantly reduced MDA levels compared to the preeclampsia group without treatment (p = 0.042, p < 0.05). However, a high dose of 200 mg/KgBW increased MDA levels, indicating a potential pro-oxidant effect. Low to moderate doses of nanocurcumin effectively reduced MDA levels in the preeclampsia model, but further studies are necessary to determine the optimal dosage and its safety in humans. Abstrak: Preeklamsia merupakan komplikasi kehamilan yang berhubungan dengan stres oksidatif dan peningkatan kadar malondialdehyde (MDA), yang menyebabkan disfungsi endotel dan gangguan perfusi plasenta. Nanokurkumin memiliki sifat antioksidan yang dapat mengurangi stres oksidatif, tetapi efektivitasnya dalam menurunkan kadar MDA pada preeklamsia masih perlu dikaji lebih lanjut. Penelitian ini bertujuan untuk mengevaluasi pengaruh pemberian nanokurkumin terhadap kadar MDA plasma pada tikus putih (Rattus norvegicus) bunting model preeklamsia yang diinduksi menggunakan Nw-Nitro-L-Arginine Methyl Ester (L-NAME). Sebanyak 24 ekor tikus Wistar bunting dibagi menjadi enam kelompok: kontrol negatif (K-), kontrol positif (K+), serta empat kelompok perlakuan yang menerima nanokurkumin dengan dosis 25 mg/KgBW (P1), 50 mg/KgBW (P2), 100 mg/KgBW (P3), dan 200 mg/KgBW (P4). Induksi preeklamsia dilakukan dengan pemberian L-NAME (125 mg/KgBW) selama enam hari, diikuti pemberian nanokurkumin. Kadar MDA plasma diukur menggunakan metode Thiobarbituric Acid (TBA) assay dan dianalisis dengan uji Kruskal-Wallis dan post-hoc Mann-Whitney (p < 0.05). Pemberian nanokurkumin 25 mg/KgBW dan 50 mg/KgBW secara signifikan menurunkan kadar MDA dibandingkan kelompok preeklamsia tanpa terapi (p = 0.042, p < 0.05). Namun, dosis tinggi (200 mg/KgBW) justru meningkatkan kadar MDA, menunjukkan efek pro-oksidan. Nanokurkumin dosis rendah hingga sedang efektif dalam menurunkan kadar MDA pada model preeklamsia, tetapi studi lanjutan diperlukan untuk menentukan dosis optimal dan keamanannya pada manusia.
The Effects of Black Garlic on Ovarian Malondialdehyde, Oviduct Muscle, and Endometrial Arterioles in Smoke-Exposed Rats Anandasari, Noni Fidya Ayu; Kridayanti, Novalia; Kaspirayanthi, Ni Ketut Devy; Nurseta, Tatit; Raras, Tri Yudani Mardining; Khotimah, Husnul; Reksohusodo, Subandi; Anita, Kenty Wantri; Yudhanto, Hendy Setyo
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1533

Abstract

The effect of cigarette smoke exposure on reproductive health has been widely studied, showing various physiological disorders induced by free radicals and oxidative stress. This study aimed to assess the protective effect of ethanol extract of black garlic (Allium sativum) on some reproductive parameters of female rats Rattus norvegicus exposed to subacute cigarette smoke. A proper experimental method with a post-test-only control group design was used, involving 25 rats divided into five groups with three different doses of the extract. The rats were exposed to cigarette smoke and then treated for 28 days. Results showed a significant decrease in ovarian Malondialdehyde levels and increased fallopian tube smooth muscle thickness in the extract-treated group. In addition, black garlic extract successfully increased the number of endometrial arterioles in the group exposed to cigarette smoke and extract, in contrast to the group exposed to cigarette smoke only. This study concludes that black garlic extract has the potential to be a protective agent against oxidative damage in the reproductive system of rats exposed to cigarette smoke, with effectiveness that depends on the dose of extract given. Further studies should investigate the long-term effects and optimal dosing of black garlic extract in humans. Additionally, exploring the molecular mechanisms behind its protective properties could enhance its application in reproductive health management.                 
Analysis of the Effect of Black Garlic (Allium sativum) Extract on Ovarian Follicular Atresia, Endometrial VEGF Expression, and Fallopian Tube Epithelial Cell Count in Rats (Rattus norvegicus) Exposed to Cigarette Smoke Kridayanti, Novalia; Anandasari , Noni Fidya Ayu; Kaspirayanthi, Ni Ketut Devy; Nurseta, Tatit; Raras, Tri Yudani Mardining; Khotimah, Husnul; Reksohusodo, Subandi; Angelica, Aina; Anita, Kenty Wantri; Yudhanto, Hendy Setyo
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1535

Abstract

Cigarette smoke exposure significantly impairs reproductive function in Rattus norvegicus. This study evaluated the protective effects of black garlic extract against such damage. Using a post-test-only control group design, 25 female Wistar rats were divided into five groups and exposed to cigarette smoke with or without varying doses of black garlic extract. After four weeks, results indicated that black garlic extract significantly increased Vascular Endothelial Growth Factor expression, enhanced fallopian tube secretory epithelial cell counts, and reduced ovarian follicular atresia in rats exposed to cigarette smoke. The group receiving 50 mg/kgBW of black garlic extract showed the most significant improvements. Statistical analysis, including One-way ANOVA, revealed significant differences between groups. Normality was assessed using the Shapiro-Wilk test, and homogeneity was confirmed with the Levene test. Significant decreases in ovarian follicular atresia (p < 0.05) and increases in Vascular Endothelial Growth Factor expression (p < 0.05) and secretory epithelial cell counts (p < 0.05) were observed in the 50 mg/kgBW treatment group compared to controls. In conclusion, black garlic extract offers dose-dependent protection against cigarette smoke-induced reproductive damage, with 50 mg/kgBW being the optimal dose. Further research should explore molecular mechanisms, long-term toxicity, and clinical applications in humans.              
Evaluation of Hematotoxicity in Female Wistar Rats Following Sub-Acute Inhalation Exposure to Polyethylene Microplastic Sulistomo, Hikmawan Wahyu; Setyowati, Anisa; Situmorang, Melani Chysti; Sulistiani, Ita; Wardani, Dewi Azar Nuria; Gusti, Kharisma Ciptaning; Nurdiana, Nurdiana; Kusuma, Ihda Dian; Rahardjo, Bambang; Reksohusodo, Subandi
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1585

Abstract

Polyethylene (PE) becomes a source of microplastics that can be widely distributed through the digestive and respiratory systems. However, its effects on blood cells are still being investigated. This study aims to analyze the impact of Polyethylene Microplastic (PE-MPs) exposure on the blood of female rats, including erythrocytes, leukocytes, and platelets. This study used female Wistar rats, which were divided into control and PE-MP groups. PE-MP was administered via whole-body inhalation at a concentration of 15 mg/m³ for 4 hours daily for 28 days. The absorption of plastic particles detected in the human bloodstream is likely to occur through mucosal contact (either through ingestion or inhalation). After the exposure period, the rats were euthanized to collect blood samples through the heart. A complete blood count was performed using an automatic hematology analyzer, and blood morphology was analyzed using thin blood smears. This study used the Mann-Whitney test. PE-MP exposure increased erythrocyte and platelet counts without a corresponding rise in leukocytes. Erythrocytes showed abnormal morphology (12.73% with ovalocytes and tear-shaped cells). Erythrocyte indices (MCV, MCH, MCHC) showed no significant differences. Platelet count rose by 1.7% (p-value= 0.017). Leukocyte and neutrophil counts were lower (0.84 and 0.94 times lower, respectively), while lymphocytes and monocytes were higher (1.03 and 1.61 times higher, respectively) in the PE-MP group compared to controls. The neutrophil-to-lymphocyte ratio did not differ significantly. PE-MP exposure in rats disrupts blood parameters, altering erythrocyte morphology and increasing platelet counts. Potential causes include oxidative stress, immune responses, and compensatory mechanisms. Study limitations include a small sample size and exclusive focus on inhalation exposure. Integrating multiple exposure routes (inhalation, ingestion, dermal) could offer a broader view of microplastic impacts. Future research with larger samples, diverse doses and durations, and exploration of additional markers or organ-specific effects is crucial for understanding PE-MP toxicity in real-world scenarios.               
Effect of Curcumin Nanoemulsion on Insulin Levels and Malondialdehyde Concentration in Zebrafish Embryos Model of Gestational Diabetes Mellitus Subandi, Subandi; Paramita, Galuh Anggarda; Khoir, Safa’atul; Nurdiana, Nurdiana; Wiyasa, I Wayan Arsana
Jurnal Ilmu Kedokteran dan Kesehatan Vol 12, No 11 (2025): Volume 12 Nomor 11
Publisher : Prodi Kedokteran Fakultas Kedokteran Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jikk.v12i11.21464

Abstract

Gestational Diabetes (GD) is a condition characterized by increased blood sugar levels (hyperglycemia) in pregnant women who were previously undiagnosed with diabetes. Hyperglycemia disrupts the performance of pancreatic β cells that are responsible for producing the hormone insulin. Curcumin functions as an anti-inflammatory, allowing the insulin reaction to occur more quickly and insulin production to increase. Methods. Post Test Only Control Group Design was used in this experimental study which was tested on each research variable consisting of 1000 zebrafish embryos divided into 5 treatment groups with each sample containing 40 embryos, consisting of a negative control, a positive control exposed to 3% glucose, and a diabetic group given curcumin nanoemulsion with three different doses (0.3125 μg/ml, 0.625 μg/ml, 1.25 μg/ml). The exposure was carried out at 2-50 hpf and then measured using INS ELISA Kit and MDA ELISA Kit. The results were analyzed using a one-way ANOVA test. Results. NOVA showed p = 0.254 for insulin levels (not significant) and p = 0.029 for MDA concentrations. Post-hoc analysis revealed that the significant difference occurred only between the negative (K−) and positive (K+) control groups (p = 0.012), while the curcumin-treated groups (K1, K2, K3) showed no statistically significant differences compared with either control group (p > 0.05). Conclusion. Curcumin nanoemulsion tended to increase insulin levels and reduce MDA concentrations, but these effects were not statistically significant (p > 0.05). The induction of hyperglycemia with 3% glucose successfully established oxidative stress in zebrafish embryos, validating the model. Further studies with larger sample sizes and molecular analyses are needed to confirm the biological trends observed.
Co-Authors AA Sudharmawan, AA Abd. Rasyid Syamsuri Aina Angelica Akhmalia, Qorry' Afyu Anandasari , Noni Fidya Ayu Anandasari, Noni Fidya Ayu Andriani, Lela Dwi Angelica, Aina Anisa Setyowati Aris Widayati Arista, Devi Maya Asih, Aliyah Tresna Astri Proborini Azahra, Mutiara Syifa Balindra, Fredlina Rossa Bambang Rahardjo Chatarina Umbul Wahyuni Daniel Happy Putra Dewi Ariani Dewi Azar Nuria Wardani Dian Hanifarizani, Rahma Fathir, Nabila Gizta, Aura Bella Gusti, Kharisma Ciptaning Hendy Setyo Yudhanto Hikmawan Wahyu Sulistomo Husnul Khotimah Husnul Khotimah Ihda Dian Kusuma Ihda Dian Kusuma Ita Sulistiani Kaspirayanthi, Ni Ketut Devy Kenty Wantri Anita Kenty Wantri Anita, Kenty Wantri Kharisma Ciptaning Gusti Khoir, Safa’atul Kridayanti, Novalia Kusworini Handono Maharani, Wening Pangesthi Melani Chysti Situmorang Nasution, Annio Indah Lestari Ni Ketut Devy Kaspirayanthi Noni Fidya Ayu Anandasari Novalia Kridayanti Nurdiana Nurdiana Nurdiana Nurdiana Nurdiana Ovinery, Amarsya Paramita, Galuh Anggarda Priyahita, Fatnuraini Rismaina Putri Rizki Amelia Safitri, Risma Andani Ayu Sahara, Aulia Ilma Sari, Laras Indah Savitri, Faradea Ubaidurrohmah Setyowati, Anisa Sigit Purbadi Silvani, Yulia Sisca Dewi Karlina, Sisca Dewi Situmorang, Melani Chysti Subakti, Nastyazka Sulistiani, Ita Sulistomo, Hikmawan Wahyu Syamsiatul Akhyar Riyadz Syarafina, Hafizhah Dini Tatit Nurseta Tatit Nurseta Tri Yudani Mardining Raras Tri Yudani MR Wardani, Dewi Azar Nuria Wati, Linda Ratna Wiyasa, I Wayan Arsana Yudhanto, Hendy Setyo