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All Journal Jurnal Sains dan Seni ITS BIOTROPIA - The Southeast Asian Journal of Tropical Biology Journal of Tropical Biodiversity and Biotechnology Akta Kimia Indonesia Biosaintifika: Journal of Biology & Biology Education Zeta - Math Journal Jurnal Abdi: Media Pengabdian Kepada Masyarakat Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Sewagati Biosaintifika: Journal of Biology & Biology Education
Triono Bagus Saputro, Triono Bagus
Biology Departement, Faculty Of Science, Sepuluh Nopember Institute Of Technology, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Public Health Social Sciences Veterinary Other

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Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae Dini Ermavitalini; Niki Yuliansari; Endry Nugroho Prasetyo; Triono Bagus Saputro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.6783

Abstract

Botryococcus sp. is one of microalgae species that has a high lipid content as much as 75% of their dry weight. But, lipid production by microalgae is regulated by their environmental condition (pH, light, temperature, nutrition, etc). Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter the Botryococcus sp. genetic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. Botryococcus sp. was irradiated with different doses of gamma ray of 60Co  (0, 2, 4, 6, and 10 Gy),  and the effect  on the growth, lipid content, and fatty acid composition of microalgae were observed. Research design used is random complete (RAL) with 95 %  confident level for quantitive analysis based on the biomass and lipid contents. More over fatty acid composition was analyzed by Gas Cromatography-Mass Spectrometry (GC-MS). Results showed that Gamma irradiated gave an effect on growth and lipid content of Botryococcus sp. But between the control treatment (0 Gy) with microalgae irradiated dose of 2 Gy, 4 Gy and 6 Gy were not significantly different. Whereas between the control with 10 Gy irradiated was significantly different. The highest biomassa and lipid content are found in 10 Gy irradiated microalgae with 0.833 gram biomass and 41% lipid content. Fatty acid profile of Botryococcus sp. control has 6 fatty acids while 10 Gy irradiated microalgae has 12 fatty acids, with the long-chain fatty acids increased, whereas short-chain fatty acids decreased.
In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD Triono Bagus Saputro; Fathin Finariyah; Siti Dianawati; Nur Fadlilatus Sholihah; Dini Ermavitalini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6934

Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2.4 D. The result show that 4 ppm of 2.4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contain NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66.7%. Furthermore, Manding variety shows a better performance in callus weight improvement with 170 mg, while Talango shows no improvement of callus weight. The result of RAPD analysis indicate that the genome characteristic were different between initial callus and surviving callus. Out of ten primers, only five primers shows polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. Manding can be further developed as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.How to CiteSaputro, T. B., Finariyah, F., Dianawati, S., Sholihah, N. F., Ermavitalini, D. (2016). In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD. Biosaintifika: Journal of Biology Biology Education, 8(3), 344-352. 
Prediksi Produksi Kelapa di Kabupaten Banyuwangi Dengan Algoritma Neural Network Herlambang, Teguh; Nurhadi, Hendro; Ermavitalini, Dini; Huda, Miftahul; Oktavianto, Lucky; Saputro, Triono Bagus; Arief, M. Romli
Zeta - Math Journal Vol 9 No 2 (2024): November
Publisher : Universitas Islam Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/zeta.2024.9.2.90-96

Abstract

Salah satu daerah penghasil kelapa terbesar di Indonesia adalah Banyuwangi, yang terletak di ujung timur Pulau Jawa. Perkebunan kelapa di Banyuwangi tidak hanya meningkatkan pendapatan petani, tetapi juga memajukan industri pengolahan lokal. Banyuwangi memiliki area potensial yang akan membantu pertumbuhan bisnis kelapa dan agroindustri lainnya. Potensi budidaya kelapa tersebar di berbagai wilayah Banyuwangi, dengan beberapa kecamatan yang menonjol dalam hal produksi dan pengolahan kelapa. Banyuwangi juga memiliki tanah yang subur, iklim yang mendukung, dan sumber daya manusia yang berpengalaman dalam budidaya kelapa Tujuan dari penelitian ini adalah untuk meramalkan produksi kelapa di Kabupaten Banyuwangi dan memberikan informasi yang akurat tentang perkiraan jumlah produksi di masa depan. Diharapkan hasil ini akan membantu petani dan pemangku kebijakan membuat keputusan yang tepat tentang manajemen produksi, distribusi, dan pengembangan industri kelapa di daerah tersebut untuk meningkatkan keberlanjutan, keuntungan, dan efisiensi. Pada penelitian ini menggunakan algoritma Neural Network guna menunjukkan kinerja terbaik pada pengujian dengan pembagian data pelatihan (Tr) sebesar 70%, 80%, dan 90%, serta data pengujian (Ts) sebesar 30%, 20%, dan 10%. Hasil pada implementasi algoritma Neural Network sebagai peramalan produksi kelapa pada banyuwangi menghasilkan RMSE berturut-turut sebesar 0,039, 0,036, dan 0,036.
Identification of Microalgae Isolates Using 18 S rRNA Markers and Testing Their Antioxidant Capacity Ermavitalini, Dini; Rahmahana, Ratna Syifa'a; Saputro, Triono Bagus; Rizam, Bilqis Naura Safira; Purnobasuki, Hery; Ni'matuzahroh
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 1 (2025): April 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i1.19919

Abstract

Microalgae are photoautotrophic microorganisms that synthesize bioactive compounds, including antioxidant compounds. Exploratory research on microalgae with strong antioxidant capacity as free radical scavengers is interesting and important to do. This study aims to identify and evaluate the antioxidant capacity of microalgae. Water samples were diluted with multilevel dilution. Microalgae were isolated using the streak plate method, and four microalgae isolates were successfully cultivated in vitro. Identification was carried out by phylogenetic analysis based on 18S rRNA marker gene sequences, namely Chlorella vulgaris, Desmodesmus armatus, Dictyosphaerium ehrenbergianum, and Vitreochlamys incisa. Antioxidant capacity was evaluated using three methods, namely DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonate)), and FRAP (Ferric Reducing Antioxidant Power). IC50 as the concentration required to inhibit 50% of free radicals showed that methanol extract of C. vulgaris had a strong antioxidant capacity in the ABTS test with IC50 of 81.693 ppm and the highest in the DPPH test with IC50 of 297.451 ppm. In the FRAP test, the highest antioxidant capacity was in ethanol extract of D. ehrenbergianum isolate of 74.45 mg AAE/g. The D. armatus isolate had the lowest antioxidant capacity in the ABTS, DPPH, and FRAP tests.
FATTY ACID PROFILES OF MICROALGAE ISOLATES FROM THE WATERS OF ITS DORMITORY POND IN SURABAYA, INDONESIA Mawardani, Alfiyyana Nurrahma; Ermavitalini, Dini; Saputro, Triono Bagus; Muzaki, Farid Kamal; Purnomo, Adi Setyo; Purnobasuki, Hery
BIOTROPIA Vol. 32 No. 1 (2025): BIOTROPIA Vol. 32 No. 1 April 2025
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2025.32.1.2428

Abstract

ARTICLE HIGHLIGHTS- Microalgae from ITS Dormitory Pond were identified using morphological analysis.- The study analyzed the fatty acid composition of four microalgae genera.- Dictyosphaerium had the highest total fatty acid content.- The findings suggest potential applications of these microalgae in biotechnology.- Further research is needed to improve fatty acid production. ABSTRACT Microalgae are a diverse group of autotrophic microorganisms classified into prokaryotic and eukaryotic groups. Their ability to thrive in various environmental conditions enables the identification of species with distinct fatty acid profiles. The absence of a microalgae culture collection at Sepuluh Nopember Institute of Technology (ITS) motivated the selection of the ITS Dormitory Pond as the research site. This study aimed to identify microalgae species present in the pond through morphological characterization and analyze their fatty acid profiles. Morphological identification was conducted using taxonomic references, while fatty acid composition was determined via GC-MS analysis. The results identified four genera: Dictyosphaerium, Vitreochlamys, Desmodesmus, and Chlorella. Their fatty acid profiles varied, comprising saturated (SFA), monounsaturated (MUFA), and polyunsaturated fatty acids (PUFA). Dictyosphaerium contained 17.57% SFA, 12.28% MUFA, and 0.33% PUFA, while Vitreochlamys had 10.83% SFA and 10.19% MUFA. Desmodesmus exhibited 14.19% SFA, 7.39% MUFA, and 4.14% PUFA, whereas Chlorella had 1.92% SFA and 2.67% PUFA. These findings highlight the potential of microalgae from ITS Dormitory Pond as a natural source of fatty acids. Further research is recommended to optimize culture conditions for enhancing commercially valuable fatty acid production.
Penanaman Karakter Peduli Lingkungan melalui Program Edukasi Konservasi Keanekaragaman Hayati Sejak Usia Dini Ashuri, Nova Maulidina; Sa'adah, Noor Nailis; Setiawan, Edwin; Ermavitalini, Dini; Saputro, Triono Bagus; Nurhayati, Awik Puji Dyah
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2021: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.164 KB)

Abstract

Program Pendidikan Konservasi Keanekaragaman Hayati sejak Usia Dini bertujuan untuk membangun karakter peduli lingkungan, menambah pengetahuan, minat, kepedulian dan kepedulian dalam melestarikan, melindungi dan memanfaatkan keanekaragaman hayati di sekitar lingkungannya yang dimulai sejak usia dini. Program pendidikan dilaksanakan dengan penyuluhan, simulasi iptek, dan advokasi (pendampingan). Program tersebut telah dilaksanakan di Taman Kanak-kanak Siti Masithoh dan di tempat pendidikan Al Qur'an yaitu Al Abror melalui pembuatan "Kebun Kehati" serta pelaksanaan seminar parenting dengan tema "Membangun Karakter Peduli Anak. Lingkungan Melalui Program Pelestarian Keanekaragaman Hayati Sejak Dini di Desa Boro, Kedungwaru, Tulungagung, Jawa Timur "yang dihadiri oleh siswa dan orang tua / wali siswa, perwakilan guru dari Taman Kanak-kanak dan Sekolah Dasar di desa serta para pengawas dan Kepala Sekolah Dinas Pendidikan Pendidikan pelestarian keanekaragaman hayati ini juga dapat menambah pengetahuan dan inovasi generasi penerus untuk dapat memanfaatkan, mengelola, melindungi dan melestarikan sumber daya alam sekitar.Kegiatan ini juga dilakukan dalam rangka meningkatkan kesadaran setiap individu terhadap kondisi lingkungannya.Selain itu, keberhasilan program ini juga membutuhkan kendali dari masyarakat dan partisipasi negara.
Morpho-Physiological, ISSR-Based Molecular Characterization of Gamma-Irradiated Soybean Under Waterlogging Stress Solihah, Anisatus; Indahsari, Dianita; Mahendra, Belva Aria; Fadilah, Aulia Sunan; Muslihatin, Wirdhatul; Chokwiwatkul, Ratchata; Saputro, Triono Bagus
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 3 (2025): November 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i3.31511

Abstract

Soybeans (Glycine max) are Indonesia's third-most important food crop after rice and maize. However, because of its susceptibility to waterlogging, its productivity decreases. Gamma-ray irradiation has been used to create soybean varieties that are resistant to waterlogging. This study investigates the genetic variation and morpho-physiological traits of irradiated Grobogan soybean varieties under waterlogging stress. Soybean seeds were exposed to gamma-ray doses of 0 Gy, 25 Gy, 50 Gy, 75 Gy, and 100 Gy, then the soybean plants were submerged in water at concentrations of 0%, 100%, 150%, 200%, and 250%. The study's morpho-physiological parameters include seed weight, pod count, plant height, chlorophyll content, and seed viability. The results imply that varying waterlogging levels and radiation dosages show varied responses. All irradiation doses showed optimal growth in the control treatment, whereas 75 Gy and 100 Gy under 250% waterlogging conditions showed the most severe growth inhibition. To evaluate genetic variation among the irradiated soybean variants, five ISSR markers were employed. Because of its high PIC value of 0.391, marker ISSR-1 was found to be the most effective in detecting polymorphisms. ISSR-1 is a promising marker for assessing genetic diversity in irradiated soybean plants, according to the results. These results are valuable for the development of soybean breeding strategies targeting improved tolerance to waterlogging stress.
Phenotypic and Molecular Characterization of Multishoots Development in Transgenic Phalaenopsis amabilis (L.) Blume Harboring 35s::KNAT1 (Knotted-Like Arabidopsis thaliana 1) Saputro, Triono Bagus; Semiarti, Endang; Purwantoro, Aziz
BIOTROPIA Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.615

Abstract

Phalaenopsis amabilis (L.) Blume is one of Indonesian natural orchids which has an aesthetic flower and possesses high economic value. The low multiplication rate and long period of life cycle are the main obstacles to conventionally propagate this orchid. The aims of this research were to analyze the stability of transgenic plant P. amabilis harboring 35S::KNAT1 based on morpho-genomic characterization. KNAT1 gene is reported as a gene that is involved in shoot formation, and it had been successfully introduced into Phalaenopsis amabilis (L.) Blume genome. After seven times regeneration, the confirmation of the transgene existence in the genome is needed to ensure whether the plant could consistently maintain the transgene in its genome and to characterize the shoot development. The experiment was carried out in 3 steps: 1) Co-integration analysis of 35S::KNAT1 into P. amabilis genome; 2) Phenotypic analysis on the multiplication rate, morphological variation, and venation pattern; and 3) Protein profile analysis of transgenic plants. The results showed that the survival rate of putative transgenic was 58.7% on NP0 medium and 62.5% on NP SIM medium. PCR analysis confirmed that 82.5% transgenic growth on NP0 and 93.33% on NP SIM contained DNA fragments of KNAT1 gene, NPTII gene and trnL-F intergenic spacer, indicating that those plants are positive transgenic. The 35S::KNAT1 transgenes and phytohormone were independently involved in multishoot formation of P. amabilis transgenic plants. The phenotypes of plantlets were classified into six main criteria, i.e., normal shape, lobed leaves, rosette, elongated stem, cup shoot, and widened leaves. The normal type was the most abundant type of variation (±29%) in both media. Protein profile showed that all transgenic plants produced 45.8 kDa protein, and that was equivalent with molecular weight of KNAT1 protein. Taken together, all those data indicated that 35S::KNAT1 transgene was consistently integrated into the transgenic plant genome.
Co-Authors Abdul Wahab Abdul Wahab Adi Setyo Purnomo Arief, M. Romli Aunurohim Aunurohim Awik Puji Dyah Nurhayati Aziz Purwantoro Belva Ariya Mahendra Chokwiwatkul, Ratchata Dianita Indahsari Dini Ermavitalini Dwi Yanuar Rakhma Sari Edwin Setiawan Eka Afiyanti Rohmah Elena Rosalia Firsta Endang Semiarti Endry Nugroho Prasetyo Fadilah, Aulia Sunan Faradiba Lazuardi Rohman Farid Kamal Muzaki Fathin Finariyah Fathin Finariyah, Fathin Hendro Nurhadi Hery Purnobasuki Indah Trisnawati Dwi Tjahjaningrum Indahsari, Dianita Irine Prabhandaru Iska Desmawati Khoirun Nisak Kristanti Indah Purwani Leonardo Pascalis Dimas Cahyo Baskoro Mahendra, Belva Aria MARIA BINTANG Mawardani, Alfiyyana Nurrahma Miftahul Huda Muhibuddin, Anton Ni'matuzahroh Niki Yuliansari Niki Yuliansari, Niki Ni’matuzahroh Ni’matuzahroh Noor Nailis Saadah Noor Nailis Sa’adah Nova Maulidina Ashuri Nur Fadlilatus Sholihah Nur Fadlilatus Sholihah Nur Fadlilatus Sholihah, Nur Fadlilatus Nurul Jadid Oktavianto, Lucky Pinka Langlangdewi Nurrachmamila Rahmahana, Ratna Syifa'a Rizam, Bilqis Naura Safira Rossy Angelina Latuharhary Sa'adah, Noor Nailis Sa’adah, Noor Nailis Saptarini, Dian Setyo Nugroho Shovitri, Maya Siska Yulia Rukhmana Siti Dianawati Siti Dianawati, Siti Solihah, Anisatus Sri Nurhatika Sumarni Dwirejeki Teguh Herlambang Thalita Meidina Vita Siti Fatimah Yusmani Prayogo