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All Journal Jurnal Sains dan Seni ITS Journal of Tropical Biodiversity and Biotechnology Jurnal Penelitian Pendidikan IPA (JPPIPA) Akta Kimia Indonesia Biosaintifika: Journal of Biology & Biology Education Zeta - Math Journal Jurnal Abdi: Media Pengabdian Kepada Masyarakat Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Sewagati Rumphius Pattimura Biological Journal
Triono Bagus Saputro, Triono Bagus
Biology Departement, Faculty Of Science, Sepuluh Nopember Institute Of Technology, Surabaya, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae Dini Ermavitalini; Niki Yuliansari; Endry Nugroho Prasetyo; Triono Bagus Saputro
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.6783

Abstract

Botryococcus sp. is one of microalgae species that has a high lipid content as much as 75% of their dry weight. But, lipid production by microalgae is regulated by their environmental condition (pH, light, temperature, nutrition, etc). Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter the Botryococcus sp. genetic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. Botryococcus sp. was irradiated with different doses of gamma ray of 60Co  (0, 2, 4, 6, and 10 Gy),  and the effect  on the growth, lipid content, and fatty acid composition of microalgae were observed. Research design used is random complete (RAL) with 95 %  confident level for quantitive analysis based on the biomass and lipid contents. More over fatty acid composition was analyzed by Gas Cromatography-Mass Spectrometry (GC-MS). Results showed that Gamma irradiated gave an effect on growth and lipid content of Botryococcus sp. But between the control treatment (0 Gy) with microalgae irradiated dose of 2 Gy, 4 Gy and 6 Gy were not significantly different. Whereas between the control with 10 Gy irradiated was significantly different. The highest biomassa and lipid content are found in 10 Gy irradiated microalgae with 0.833 gram biomass and 41% lipid content. Fatty acid profile of Botryococcus sp. control has 6 fatty acids while 10 Gy irradiated microalgae has 12 fatty acids, with the long-chain fatty acids increased, whereas short-chain fatty acids decreased.
In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD Triono Bagus Saputro; Fathin Finariyah; Siti Dianawati; Nur Fadlilatus Sholihah; Dini Ermavitalini
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.6934

Abstract

Maize (Zea mays) is one of graminae plants that widely spread for many purposes wheter in food industry, feed, or bioenergy. Those high utilization required an increment in production, but unfortunately the needs were not meet to the demands since conversion of agricultural area increase lately. Indonesia has 99.093 km of shoreline that recognize as marginal land where the salinity is high as well. This research try to obtain tolerant variant from two local cultivars that planted in Madura Island. Manding and Talango varieties were used as an explant for callus induction stage in MS supplemented with 2.4 D. The result show that 4 ppm of 2.4 D were the best concentration to induce the callus in both varieties. The induced callus were exposed to medium MS that contain NaCl (0, 2500, 5000, and 7500 ppm). In 7500 ppm of NaCl, Manding variety has 100% of surviving callus, while Talango variety only 66.7%. Furthermore, Manding variety shows a better performance in callus weight improvement with 170 mg, while Talango shows no improvement of callus weight. The result of RAPD analysis indicate that the genome characteristic were different between initial callus and surviving callus. Out of ten primers, only five primers shows polymorphism i.e OPA 13, OPB 07, OPC 02, OPK 20, and OPU 19. Manding can be further developed as high tolerance variety in Salinity stress, thus it proposed to be developed furthermore.How to CiteSaputro, T. B., Finariyah, F., Dianawati, S., Sholihah, N. F., Ermavitalini, D. (2016). In Vitro Selection of Local Maize (Zea mays) on NaCl Stress and Its Genetic Characterization using RAPD. Biosaintifika: Journal of Biology Biology Education, 8(3), 344-352. 
Prediksi Produksi Kelapa di Kabupaten Banyuwangi Dengan Algoritma Neural Network Herlambang, Teguh; Nurhadi, Hendro; Ermavitalini, Dini; Huda, Miftahul; Oktavianto, Lucky; Saputro, Triono Bagus; Arief, M. Romli
Zeta - Math Journal Vol 9 No 2 (2024): November
Publisher : Universitas Islam Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31102/zeta.2024.9.2.90-96

Abstract

Salah satu daerah penghasil kelapa terbesar di Indonesia adalah Banyuwangi, yang terletak di ujung timur Pulau Jawa. Perkebunan kelapa di Banyuwangi tidak hanya meningkatkan pendapatan petani, tetapi juga memajukan industri pengolahan lokal. Banyuwangi memiliki area potensial yang akan membantu pertumbuhan bisnis kelapa dan agroindustri lainnya. Potensi budidaya kelapa tersebar di berbagai wilayah Banyuwangi, dengan beberapa kecamatan yang menonjol dalam hal produksi dan pengolahan kelapa. Banyuwangi juga memiliki tanah yang subur, iklim yang mendukung, dan sumber daya manusia yang berpengalaman dalam budidaya kelapa Tujuan dari penelitian ini adalah untuk meramalkan produksi kelapa di Kabupaten Banyuwangi dan memberikan informasi yang akurat tentang perkiraan jumlah produksi di masa depan. Diharapkan hasil ini akan membantu petani dan pemangku kebijakan membuat keputusan yang tepat tentang manajemen produksi, distribusi, dan pengembangan industri kelapa di daerah tersebut untuk meningkatkan keberlanjutan, keuntungan, dan efisiensi. Pada penelitian ini menggunakan algoritma Neural Network guna menunjukkan kinerja terbaik pada pengujian dengan pembagian data pelatihan (Tr) sebesar 70%, 80%, dan 90%, serta data pengujian (Ts) sebesar 30%, 20%, dan 10%. Hasil pada implementasi algoritma Neural Network sebagai peramalan produksi kelapa pada banyuwangi menghasilkan RMSE berturut-turut sebesar 0,039, 0,036, dan 0,036.
Penanaman Karakter Peduli Lingkungan melalui Program Edukasi Konservasi Keanekaragaman Hayati Sejak Usia Dini Ashuri, Nova Maulidina; Sa'adah, Noor Nailis; Setiawan, Edwin; Ermavitalini, Dini; Saputro, Triono Bagus; Nurhayati, Awik Puji Dyah
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2021: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.164 KB)

Abstract

Program Pendidikan Konservasi Keanekaragaman Hayati sejak Usia Dini bertujuan untuk membangun karakter peduli lingkungan, menambah pengetahuan, minat, kepedulian dan kepedulian dalam melestarikan, melindungi dan memanfaatkan keanekaragaman hayati di sekitar lingkungannya yang dimulai sejak usia dini. Program pendidikan dilaksanakan dengan penyuluhan, simulasi iptek, dan advokasi (pendampingan). Program tersebut telah dilaksanakan di Taman Kanak-kanak Siti Masithoh dan di tempat pendidikan Al Qur'an yaitu Al Abror melalui pembuatan "Kebun Kehati" serta pelaksanaan seminar parenting dengan tema "Membangun Karakter Peduli Anak. Lingkungan Melalui Program Pelestarian Keanekaragaman Hayati Sejak Dini di Desa Boro, Kedungwaru, Tulungagung, Jawa Timur "yang dihadiri oleh siswa dan orang tua / wali siswa, perwakilan guru dari Taman Kanak-kanak dan Sekolah Dasar di desa serta para pengawas dan Kepala Sekolah Dinas Pendidikan Pendidikan pelestarian keanekaragaman hayati ini juga dapat menambah pengetahuan dan inovasi generasi penerus untuk dapat memanfaatkan, mengelola, melindungi dan melestarikan sumber daya alam sekitar.Kegiatan ini juga dilakukan dalam rangka meningkatkan kesadaran setiap individu terhadap kondisi lingkungannya.Selain itu, keberhasilan program ini juga membutuhkan kendali dari masyarakat dan partisipasi negara.
Exploring Oxygenated Sesquiterpenes from Merauke Agarwood as PBP1a and PBP3 Inhibitors in Acinetobacter baumanii: An In-Silico Approach Yulianto Ade Prasetya; Triono Bagus Saputro; Yohanes Ardian Kapri Negara; Arif Luqman
Jurnal Penelitian Pendidikan IPA Vol 12 No 3 (2026)
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v12i3.14627

Abstract

Acinetobacter baumannii is a multidrug-resistant pathogen causing severe nosocomial infections, primarily associated with alterations in penicillin-binding proteins (PBPs) that reduce the effectiveness of β- lactam antibiotics. Despite increasing interest in plant-derived bioactive compounds, the potential of oxygenated sesquiterpenes from Aquilaria malaccensis as inhibitors of PBPs remains underexplored, indicating a clear research gap. This study aimed to evaluate the antibacterial potential of three oxygenated sesquiterpenes—Agaruspirol, Eudesmol, and Sinenofuranol—against PBP1a (3UE3) and PBP3 (3UDF). A systematic literature review (SLR) integrated with an in silico approach was conducted, including ligand retrieval from PubChem, ADMET prediction using ADMETlab 3.0, molecular docking using CB-Dock2, interaction analysis with UCSF ChimeraX and PLIP, and antibacterial activity prediction using PASS Online. The results showed that all compounds satisfied Lipinski’s rule of five and exhibited favorable ADMET profiles. Molecular docking demonstrated stable interactions with both PBPs, dominated by hydrogen bonding and hydrophobic interactions. Agaruspirol exhibited the strongest binding affinity toward PBP3 (–6.9 kcal/mol) and PBP1a (–6.5 kcal/mol), along with the highest predicted antibacterial activity (Pa = 0.364; Pi = 0.039). These findings suggest that oxygenated sesquiterpenes, particularly agaruspirol, have potential as lead compounds for the development of antibacterial agents targeting PBP-mediated resistance in A. baumannii, and provide a scientific basis for further in vitro and in vivo validation.
DNA Barcoding Application Using CYTB (Cytochrome-B) Marks for Authentication of Patin Fish (Pangasius sp.) Products in Surabaya Area Aulia, Wina Intan Nur; Setiawan, Edwin; Triwibowo, Radestya; Saputro, Triono Bagus; Hidayati, Dewi; Nurhayati, Awik Puji Dyah; Ridho, Muhammad Ainur Rosyid
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p079-092

Abstract

DNA barcoding is a practical and accurate molecular technique for identifying processed fish products that lack morphological characteristics. However, its application is limited by the availability of reference databases, DNA quality, and primer suitability. This study aims to determine the genetic identity of pangasius catfish (Pangasius sp.) marketed in Surabaya using the Cytochrome b (CytB) gene and to evaluate PCR-RFLP as an alternative to sequencing. A total of twenty samples (fresh and frozen fillets) were analyzed through DNA extraction and PCR amplification of the CytB gene, followed by in silico and in vitro analyses.The results show that CytB demonstrates high amplification success and provides sufficient resolution for species-level identification based on Neighbor-Joining analysis with the Kimura 2-parameter model. The study confirms that the samples were genetically identified as Pangasius pangasius and Pangasius hypophthalmus. This research highlights the novelty of integrating in silico prediction with laboratory validation to improve marker evaluation in DNA barcoding studies. Furthermore, it demonstrates that CytB-based PCR-RFLP is a rapid and cost-effective alternative to sequencing for detecting species mislabeling. Overall, the CytB gene is proven to be a reliable molecular marker for species authentication, supporting traceability systems and strengthening seafood authenticity monitoring in commercial markets.
Co-Authors Abdul Wahab Abdul Wahab Arief, M. Romli Arif Luqman Aulia, Wina Intan Nur Aunurohim Aunurohim Awik Puji Dyah Nurhayati Belva Ariya Mahendra Dewi Hidayati Dianita Indahsari Dini Ermavitalini Dwi Yanuar Rakhma Sari Edwin Setiawan Eka Afiyanti Rohmah Elena Rosalia Firsta Endry Nugroho Prasetyo Faradiba Lazuardi Rohman Farid Kamal Muzaki Fathin Finariyah Fathin Finariyah, Fathin Hendro Nurhadi Hery Purnobasuki Indah Trisnawati Dwi Tjahjaningrum Irine Prabhandaru Iska Desmawati Khoirun Nisak Kristanti Indah Purwani Leonardo Pascalis Dimas Cahyo Baskoro MARIA BINTANG Miftahul Huda Muhibuddin, Anton Niki Yuliansari Niki Yuliansari, Niki Ni’matuzahroh Ni’matuzahroh Noor Nailis Saadah Noor Nailis Sa’adah Nova Maulidina Ashuri Nur Fadlilatus Sholihah Nur Fadlilatus Sholihah Nur Fadlilatus Sholihah, Nur Fadlilatus Nurul Jadid Oktavianto, Lucky Pinka Langlangdewi Nurrachmamila Ridho, Muhammad Ainur Rosyid Rossy Angelina Latuharhary Sa'adah, Noor Nailis Sa’adah, Noor Nailis Saptarini, Dian Setyo Nugroho Shovitri, Maya Siska Yulia Rukhmana Siti Dianawati Siti Dianawati, Siti Sri Nurhatika Sumarni Dwirejeki Teguh Herlambang Thalita Meidina Triwibowo, Radestya Vita Siti Fatimah Yohanes Ardian Kapri Negara Yulianto Ade Prasetya Yusmani Prayogo