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Plant Chromosome and Karyotype Analysis Training for High School/MA/Equivalent Teachers in Samarinda and its Surrounding Areas Oktavianingsih, Linda; Aryani, Retno; Hariani, Nova; Rosadi, Imam; Pratiwi, Anggita Endar; Arif, Muhammad Fauzi
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 10, No 3 (2024): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpkm.88892

Abstract

Increasing biology teachers’ comprehension of the principles of genetics is attainable through the implementation of specialized training sessions focused on chromosome and karyotype analysis. In this training, 21 high school biology teachers from Samarinda and Nears participated in a structured training program encompassing three stages. In the initial phase, participants were provided with materials on the fundamental aspects of genetics. Subsequently, in the second phase, hands-on training was imparted, focusing on acquiring skills in plant chromosome preparation techniques. Lastly, the third stage concentrated on refining skills to construct chromosome karyotyping. Completing all stages was required for participants, followed by submitting evaluation forms for assessment by the committee. The evaluation outcomes revealed that 84.30% of participants regarded the presentation and assistance provided by principal lecturer and assistants as highly commendable. At the same time, the remaining respondents expressed satisfaction with the qualityand adequacy of the training. This comprehensive training effectively equipped participants with specialized chromosome and karyotype analysis skills, and the participants notably absorbed the training materials. The anticipated outcome of enhanced teacher understanding, specifically in the knowledge of inheritance and chromosome biology, was to stimulate heightened levels of student engagement and learning interest within the biology classroom.
Perkembangan Motorik Kasar Anak Melalui Permainan Modifikasi Estafet Bola Di TK IT Daarush-Shohaabah Cikoneng Raina, Ade; Sari, Amelia Cahya; Haerani, Salsya; Oktavianti, Riska Fitri; Hendasyah, Firza; Aura Maulida, Salma Azril; Oktavianingsih, Linda; Firmansyah, Andan
Kolaborasi: Jurnal Pengabdian Masyarakat Vol 4 No 1 (2024): Kolaborasi: Jurnal Pengabdian Masyarakat
Publisher : Yayasan Inspirasi El Burhani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56359/kolaborasi.v4i1.342

Abstract

Introduction: One form of education is early childhood education, where the basics are physical growth and development (fine and gross motor coordination), intelligence, social emotionality, language and communication. Gross motor skills are one aspect of a child's development that must be optimized because they are very important for maximizing the growth and development of a child's body. To develop gross motor skills in children, this can be done by playing ball relay games. Objective: The aim is to determine children's gross motor skills through the modified relay ball game at Daarush-Shohaabah Cikoneng IT Kindergarten. Method: This community service was carried out at the Daarush-Shohaabah Cikoneng IT Kindergarten with a total of 48 children participating. This community service seeks to monitor gross motor skills in children by conducting observations and intervening or treating ball relay games to improve gross motor skills. Observation of gross motor skills is carried out by dividing them into several indicators, namely not yet developing, starting to develop, developing as expected and developing very well. Result: After the ball relay game treatment was carried out, the number of undeveloped indicators decreased to 2 children (4.17%), the indicators starting to develop became 4 (8.33%), the indicators developed according to expectations, 24 children (50%), In the very well developed indicator, there was an increase of 18 (37.5%). Conclusion : The conclusion is that the implementation of this community service activity went well according to what was desired and what had been planned. This community service has a positive value for the Daarush-Shohaabah Cikoneng IT Kindergarten students. This can be seen from the enthusiasm and activeness of the participants in carrying out the ball relay game activities and the results of the observations carried out which have good values ​​for the motor skills of children at the Daarush-Shohaabah Cikoneng IT Kindergarten.
Keragaman Kultivar Lokal Ubi Jalar (Ipomoea batatas (L.) Lam) di Kabupaten Kutai Timur-Kalimantan Timur, Indonesia Maysavitrie Citra Wijayanti Kristianto; Medi Hendra; Linda Oktavianingsih
Al-Kauniyah: Jurnal Biologi Vol 17, No 1 (2024): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v17i1.30539

Abstract

 AbstrakUbi jalar (Ipomoea batatas (L.) Lam) merupakan salah satu tanaman pangan yang memiliki banyak manfaat di beberapa daerah di Indonesia termasuk di Kabupaten Kutai Timur. Informasi tentang keragaman morfologi kultivar lokal ubi jalar diperlukan untuk pemuliaan tanaman ke depannya. Penelitian dilakukan pada Maret-Juli 2021 untuk mengetahui keragaman dan hubungan kekerabatan kultivar lokal ubi jalar berdasarkan karakter morfologi. Kultivar lokal ubi jalar diambil secara purposive sampling melalui metode jelajah di 11 kecamatan, Kabupaten Kutai Timur. Karakterisasi morfologi menggunakan 32 karakter berdasarkan panduan International Board for Plant Genetic Resource (IBPGR). Analisis klaster untuk melihat hubungan kekerabatan antar kultivar menggunakan software MVSP versi 3.1. Hasil penelitian menunjukkan terdapat keragaman kultivar lokal ubi jalar yang ada di Kutai Timur berdasarkan karakter daun (bentuk, tipe cuping), umbi (bentuk, warna daging), bunga (bentuk dan warna). Hasil analisis klaster membentuk dua kelompok besar dari 23 aksesi kultivar lokal ubi jalar yang ditemukan dengan koefisien 9,671. Kelompok A dengan satu anggota, yaitu kultivar lokal 9 (Gei Meng Hom) yang terpisah dari kultivar lokal lainnya berdasarkan karakter umbi. Kultivar ini dibudidayakan oleh masyarakat asli Kalimantan (suku Dayak Wehea). Kelompok B terdiri dari 22 aksesi kultivar lokal yang dapat dikelompokkan berdasarkan karakter daun.AbstractSweet potato (Ipomoea batatas (L.) Lam) is a food crop that has many benefits in several regions in Indonesia, including East Kutai Regency. Information about the morphological diversity of local sweet potato cultivars is needed for future plant breeding. The research was conducted from March-July 2021 with the aim of determining the diversity and relationships of local sweet potato cultivars based on morphological characters. The collection of local sweet potato cultivars was carried out using purposive sampling using the roaming method in 11 sub-districts, East Kutai Regency. Morphological characterization uses 32 characters based on the International Board for Plant Genetic Resources (IBPGR) guidelines. Cluster analysis to see the kinship relationships formed between local sweet potato cultivars using MVSP software version 3.1. The results of the research show the diversity of local sweet potato cultivars in East Kutai based on leaf characters (shape, lobe type), tubers (shape, flesh color), flowers (shape and color), as well as forming two large groups based on the results of cluster analysis on 23 accessions which was found with a coefficient of 9.671. Group A with one member, namely local cultivar 9 (Gei Meng Hom) which is separated from other local cultivars based on tuber characteristics and is a local cultivar of sweet potato cultivated by the indigenous people of Kalimantan (Wehea Dayak tribe). Group B consists of 22 local cultivar accessions resulting from community cultivation which can be grouped based on leaf characteristics.  
Genetic diversity of local sweet potato cultivars (Ipomoea batatas [L.] Lam.) from Bulungan, North Kalimantan based on morphological characters OKTAVIANINGSIH, LINDA; FITRIANI, FITRIANI; HENDRA, MEDI; ARIF, MUHAMMAD FAUZI
Jurnal Natural Volume 24 Number 3, October 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jn.v24i3.40568

Abstract

Indonesia is one of the centers of sweet potato diversity worldwide. Sweet potatoes have the potential to be developed for food consumption. This research aims to determine the diversity of sweet potato cultivars, their morphological characteristics, and the relationships between local sweet potato cultivars in Bulungan Regency, North Kalimantan. The local sweet potato cultivars were selected using purposive sampling with the exploration method. The morphological characterization of the samples is based on IBPGR (International Board for Plant Genetic Resources) standards. Cluster analysis was conducted to identify the relationships using MVSP software version 3.1. The research identified 27 local sweet potato cultivars in Bulungan Regency. Morphological characterization revealed 41 characters that can differentiate each local sweet potato cultivar. The most influential characteristics include leaf shape, flower surface texture, the position of the stigma, flower surface color, back petal color, center shape and the number of lobes, primary leaf vein color, lobe type, main leaf bone color, stem internode length, and plant type. A flower shape that had not been discovered before, a pentagonal shape with curves, was identified. Cluster analysis divided the cultivars into two major groups with a coefficient range of 0.593 to 0.888 (59.3 to 88.8%). Cluster A consisted of one member, LK-7 (purple sweet potato), while Cluster B consisted of several subgroups with 26 local cultivars. The people of Bulungan Regency use these local cultivars for food, animal feed, and medicinal purposes.
Pemberdayaan Masyarakat Suwandi, Samarinda Ulu: Sampah Organik Dapur untuk Bumi dengan Eco Enzym Hariani, Nova; Kusuma, Ratna; Samsurianto, Samsurianto; Patang, Fatmawati; Oktavianingsih, Linda; Rukmi, Dijan Sunar
GLOBAL ABDIMAS: Jurnal Pengabdian Masyarakat Vol. 2 No. 1 (2022): Mei 2022, GLOBAL ABDIMAS
Publisher : Unit Publikasi Ilmiah Perkumpulan Intelektual Madani Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51577/globalabdimas.v2i1.350

Abstract

Mayoritas sampah yang dihasilkan dari semua kegiatan manusia adalah berasal dari rumah tangga. Hampir 70% sampah yang dihasilkan rumah tangga berupa sampah organik (sayur, buah). Sampah organik ini akan dikumpulkan dan ditumpuk di Tempat Pembuangan Sampah Akhir (TPSA). Penumpukan dan penimbunan sampah organik, akan menimbulkan berbagai masalah pencemaran tanah, udara dan air. Produksi gas metan dalam proses pembusukan dengan jumlah banyak akan menyebabkan terjadinya ledakan. Kerugian dan bahaya dari sampah organik ini bisa diminimalisir dengan cara mengolah dan memanfaatkan sampah organik tersebut yang dimulai dari rumah dan lingkungan masing-masing. Sampah organik segar dapat digunakan dalam pembuatan eco enzym. Eco enzym adalah cairan serba guna, berwarna kecoklatan yang dihasilkan dari fermentasi sampah organik yang masih segar dengan campuran gula tebu (gula organik) dan air. Kegiatan ini bertujuan untuk mengenalkan secara detil tentang cara pembuatan dan manfaat eco enzym kepada masyarakat di kampung KB Mandiri Suwandi, Kec. Samarinda Ulu akan sangat membantu berkurangnya sampah yang ditumbuk di TPSA. Cairan eco enzym dibuat dari sampah organik (sayur, kulit buah yang belum bususk dan berjamur) yang dicampur dengan gula organik (seperti gula tebu, moolasse, gula aren dan gula organik lainnya) lalu ditambahkan air alami. Setelah itu difermentasi selama lebih kurang 100 hari dalam kondisi anaerob/tertutup. Cairan eco enzym yang dihasilkan dapat dimanfaatkan untuk berbagai hal seperti pupuk tanaman, membersihkan toilet, sebagai desinfektan dan banyak manfaat lainnya. Setelah kegiatan hasil kepuasan peserta didapatkan rata-rata nilai kepuasan peserta adalah Sangat Baik (Skala nilai 5) sebanyak 94,5% dan Baik (skala nilai 4) 5,5%. Untuk skala 3 sampai 1 (Cukup, Kurang dan sangat kurang) adalah 0%. Dari data kuisioner dan respon semua peserta, secara keseluruhan peserta sangat antusias, merasa bermanfaat dan terbantu dengan kegiatan berbagi ilmu dan teknologi sederhana ini. Selama kegiatan berlangsung tidak ditemukan kendala yang berarti.
ANALYSIS OF DISEASE SEVERITY ON CITRUS LEAVES (CITRUS spp.) USING IMAGEJ AND PLANTIX SOFTWARE Hasanah, Melly Rya; Rosadi, Imam; Oktavianingsih, Linda; Fitriani, Nur Indah; Fera, Okta; Addin, Rosita; Ayuni, Cici Lis Qurrotun; Muhammadiyah, Muhammadiyah
Jurnal Pendidikan Matematika dan IPA Vol 14, No 2 (2023): July 2023
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jpmipa.v14i2.65294

Abstract

The purpose of this case study is to determine the severity of citrus leaves using Plantix software and ImageJ software. Based on the results of this case study, it was found that the severity of the disease using ImageJ software was on citrus leaves at the first location (10.641%), citrus leaves at the second location (53.278%), and citrus leaves at the third location (15.828%) which were generally the fruit plants caused by fungi and based on the results of Plantix software data, diseases in the first, second, and third locations of citrus leaves were caused by bacterial diseases. Based on the results of bacterial cell culture, isolates were found to be round white in color with a slight yellowness which is a characteristic of Xanthomonas axonopodis bacteria with a large number of ±12 colonies and a smaller number of ±35 colonies. Using software and imageJ can help farmers to find out the types of pests that attack citrus plants so that farmers can handle plants to minimize damage to citrus plants caused by pests.
DIVERSITY OF Citrus spp. FROM PENAJAM PASER UTARA, EAST KALIMANTAN BASED ON MORPHOLOGICAL CHARACTERS Oktavianingsih, Linda; Crisdayanti, Noviana; Arif, Muhammad Fauzi; Hendra, Medi
BIOTROPIA Vol. 31 No. 2 (2024): BIOTROPIA Vol. 31 No. 2 August 2024
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2024.31.2.1872

Abstract

ARTICLE HIGLIGHTS- This research is the first study to provide data on the diversity of Citrus spp. from North Penajam Paser Regency (IKN) area, East Kalimantan for further development and breeding program. - This research offers new information on the distribution of Citrus spp. from the North Penajam Paser Regency (IKN) area, East Kalimantan. - This research provides information on the phenetic relationships among Citrus spp. from the North Penajam Paser Regency (IKN) area, East Kalimantan, and the morphological characteristics that play the most significant role in their clustering. ABSTRACTCitrus spp. is local Indonesian fruit with high diversity and widely distributed around Indonesia country. Diversity and relationship data of Citrus spp. from Penajam Paser Utara-East Kalimantan has never been studied before. This study aimed to provide the diversity and relationship data of Citrus spp. from Penajam Paser Utara using morphological characteristics which served as the basic data for further development and breeding. The research was conducted through observation and sampling, followed by characterization and scoring of morphological data, and lastly the data analysis. The research succeeded in finding 35 accessions of Citrus spp. consisting of C. limon “Jeruk Lemon”, C. suhuensis, “Jeruk Siam”, C. aurantiifolia “Jeruk Nipis”, C. sinensis “Jeruk Manis”, C. maxima “Jeruk Bali”, C. microcarpa “Jeruk Kalamansi”, and Citrus sp. "Jeruk Sunkist" spread in sub-district Penajam, Sepaku, Babulu, and Waru. The dendrogram to clustering Citrus spp. in PPU was divided into two large clusters. Cluster A was grouped based on the character of tree habitus, cordate leaf apex, entire leaf margin, pink mesocarp color, and seed absence. Cluster B was grouped based on the character of the shrubs habitus, acute and rounded leaf apex, crenate leaf margin, white to orange mesocarp color, and numerous seeds in the fruit. This diversity made Citrus spp. from Penajam Paser Utara had high potential value for further development through a plant breeding program.
Implementation of Effective Cough Training to Improve Airway Clearance in Asthma Patients: A Nursing Care Process Case Study in the Emergency Department Oktavianingsih, Linda; Kurniawan, Rudi; Gunawan, Asep; Novianti, Elis
Jurnal Vocational Nursing Sciences (VNUS) Vol 7 No 2 (2025): JURNAL VNUS (Vocational Nursing Science)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jvnus.v7i2.842

Abstract

Asthma is a chronic respiratory disorder that remains a major public health concern, particularly in areas with high pollution levels. One of the most frequent nursing problems among asthma patients is ineffective airway clearance caused by mucus retention. Effective cough training is a non-pharmacological nursing intervention aimed at facilitating secretion removal and improving respiratory function. This case study evaluated the effectiveness of cough training in improving airway clearance in an asthma patient treated in the emergency department. A descriptive case study design using the nursing care process assessment, diagnosis, intervention, implementation, and evaluation was applied. Data were collected through interviews, physical examinations, observations, and documentation for two days. The intervention consisted of deep nasal inhalation for four seconds, breath-holding for two seconds, exhalation through pursed lips for eight seconds, followed by a controlled cough. After two days, improvements were observed in respiratory rate (from 26 to 22 breaths per minute), oxygen saturation (from 90% to 95%), and mucus expectoration. The patient was able to perform the technique independently after education and guided practice. These findings indicate that effective cough training is a simple, evidence-based nursing intervention that enhances airway clearance and oxygenation and should be integrated into emergency asthma care to prevent complications.
Etnobotani tumbuhan obat tradisional masyarakat Kutai di Kecamatan Kenohan, Kutai Kartanegara, Kalimantan Timur Titin Suharti; Medi Hendra; Muhammad Fauzi Arif; Linda Oktavianingsih
Jurnal Biologi Udayana Vol. 29 No. 1 (2025): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2025.v29.i01.p07

Abstract

Masyarakat di Pulau Kalimantan hingga saat ini masih banyak yang memanfaatkan keanekaragaman hayati tumbuhan sebagai obat tradisional, termasuk masyarakat Suku Kutai yang bermukim di Kecamatan Kenohan, Kabupaten Kutai Kartanegara. Pemanfaatan tumbuhan obat ini telah berlangsung lama, terutama oleh masyarakat yang tinggal di sekitar hutan. Penelitian ini bertujuan untuk mengidentifikasi jenis tumbuhan obat yang digunakan oleh masyarakat Suku Kutai, bagian tumbuhan yang dimanfaatkan, cara pengolahan, serta manfaatnya. Penelitian ini menggunakan metode survei deskriptif dengan teknik wawancara semi-terstruktur (semi-structured interview) yang dilengkapi dengan keterlibatan aktif peneliti dalam kegiatan masyarakat setempat melalui pendekatan Participatory Ethnobotanical Appraisal (PEA). Pengambilan sampel tumbuhan obat dilakukan dengan metode transect-walk sampling. Hasil penelitian mengidentifikasi 103 spesies tumbuhan dari 48 famili yang dimanfaatkan sebagai obat tradisional. Bagian tumbuhan yang paling banyak digunakan adalah daun sebesar 51%, diikuti oleh akar sebesar 16%, umbi sebesar 11, batang sebesar 10%, kulit batang sebesar 2%, getah sebesar 2%, buah sebesar 5%, dan biji sebesar 2% serta bunga 1%. Metode pengolahan tumbuhan obat yang umum dilakukan adalah dengan cara direbus sebesar 71%, ditumbuk sebesar 14%, diparut sebesar 9%, tanpa diolah sebesar 3%, dan diremas sebesar 3%. Tumbuhan obat ini dimanfaatkan oleh masyarakat untuk mengobati berbagai penyakit, termasuk penyakit degeneratif seperti diabetes, penyakit jantung, asam urat, dan hipertensi, serta penyakit tropis seperti sakit kepala, malaria, perawatan pasca-melahirkan, dan pendarahan.
Implementation of Effective Cough Training to Improve Airway Clearance in Asthma Patients: A Nursing Care Process Case Study in the Emergency Department Oktavianingsih, Linda; Kurniawan, Rudi; Gunawan, Asep; Novianti, Elis
Jurnal Vocational Nursing Sciences (VNUS) Vol 7 No 2 (2025): JURNAL VNUS (Vocational Nursing Science)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/jvnus.v7i2.842

Abstract

Asthma is a chronic respiratory disorder that remains a major public health concern, particularly in areas with high pollution levels. One of the most frequent nursing problems among asthma patients is ineffective airway clearance caused by mucus retention. Effective cough training is a non-pharmacological nursing intervention aimed at facilitating secretion removal and improving respiratory function. This case study evaluated the effectiveness of cough training in improving airway clearance in an asthma patient treated in the emergency department. A descriptive case study design using the nursing care process assessment, diagnosis, intervention, implementation, and evaluation was applied. Data were collected through interviews, physical examinations, observations, and documentation for two days. The intervention consisted of deep nasal inhalation for four seconds, breath-holding for two seconds, exhalation through pursed lips for eight seconds, followed by a controlled cough. After two days, improvements were observed in respiratory rate (from 26 to 22 breaths per minute), oxygen saturation (from 90% to 95%), and mucus expectoration. The patient was able to perform the technique independently after education and guided practice. These findings indicate that effective cough training is a simple, evidence-based nursing intervention that enhances airway clearance and oxygenation and should be integrated into emergency asthma care to prevent complications.