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All Journal E-Jurnal Medika Udayana Journal of Biology Education Jurnal Teknologi Laboratorium Jurnal Analis Kesehatan Jurnal Kesehatan Prima Jurnal Labora Medika Jurnal Analis Medika Biosains (JAMBS) Medicra (Journal of Medical Laboratory Science/Technology) Jurnal Ilmiah Biologi UMA (JIBIOMA) Prosiding University Research Colloquium Prosiding Seminar Nasional Unimus Biosaintifika: Journal of Biology & Biology Education Jurnal Info Kesehatan
Aprilia Indra Kartika, Aprilia Indra
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Other

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Profil Protein Ikan Kakap Merah (Lutjanus bitaeniatus) yang Direndam Ekstrak Buah Nanas (Ananas comosus) sebagai Pengawet Alami Berbasis SDS-PAGE Meliana, Monica; Kartika, Aprilia Indra; Fitria, Meutia Srikandi
Prosiding Seminar Nasional Unimus Vol 7 (2024): Transformasi Teknologi Menuju Indonesia Sehat dan Pencapaian Sustainable Development G
Publisher : Universitas Muhammadiyah Semarang

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Abstract

Ikan kakap merah (Lutjanus bitaeniatus) biasa dikonsumsi masyarakat karena kandungan protein tinggi,tetapi ikan mudah membusuk karena kandungan air yang cukup tinggi. Buah nanas mengandung enzimbromelin yang dapat digunakan untuk pengawet alami dengan menghambat proses pembusukan. Penelitianini bertujuan untuk menganalisis profil protein ikan kakap merah yang direndam dengan ekstrak buah nanassebagai pengawet alami berbasis SDS-PAGE. Penelitian ini adalah penelitian deskriptif dengan objekpenelitian adalah ikan kakap. Metode penelitian ini adalah perhitungan konsentrasi protein dengan metodeBradford dan profil protein menggunakan SDS-PAGE. Hasil penelitian menunjukkan bahwa konsentrasitotal protein ikan kakap yang direndam dengan buah nanas konsentrasi 70% lebih tinggi dibandingkandengan konsentrasi 80% dan 90 %. Hasil dari SDS-PAGE menunjukkan bahwa terdapat perubahan pitaprotein dibandingkan kontrol. Pita protein semakin menipis dari perendaman ekstrak buah nanas 70%, 80%,dan 90%. Kesimpulan penelitian ini adalah semakin tinggi konsentrasi ekstrak buah nanas, makakonsentrasi semakin rendah dan protein menjadi terdenaturasi.Abstrak ditulis dalam Bahasa Indonesia.  Kata Kunci : Kakap merah, Nanas, Profil protein, SDS-PAGE
Potensi Ekstrak Daun Salam (Syzygium polyanthum) sebagai Pengawet Alami Ikan Kakap Merah (Lutjanus bitaeniatus) berbasis SDS-PAGE Fitria, Meutia Srikandi; Nikmah, Khofifatun; Kartika, Aprilia Indra
Jurnal Ilmiah Biologi UMA (JIBIOMA) Vol 7, No 1 (2025): May 2025
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/jibioma.v7i1.6154

Abstract

Red snapper is a source of animal protein with a highwater content so it is easily spoiled. Decay can be prevented by preservation such as natural and artificial preservatives. Natural preservatives can use natural ingredients such as bay leaves. Bay leaves can be used as a natural preservative because they contain antibacterial substances that can inhibit the growth of spoilage bacteria. The ability of bay leaves as a preservative can be seen with the SDS-PAGE method by looking at the protein profile. This study aims to determine the potential of bay leaf extract as a natural preservative in SDS-PAGE-based red snapper. This type of research is experimental based on the results of protein concentration, organoleptic test, and protein profile of SDS-PAGE results. There were 5 treatments of red snapper fish, namely fresh fish, fish without soaking stored 24 hours at room temperature, and fish with bay leaf extract with a concentration of 5%, 15%, and 25% for 30 minutes and then stored for 24 hours at room temperature.  The results showed that the concentration of bay leaf extract that has the potential as a natural red snapper is 25%. This is because the protein concentration with this concentration is the highest (3.7226 µg/ µL), the best organoleptic test, and the SDS-PAGE results are almost the same protein band as the fresh control. This study concludes that bay leaf extract has the potential to be used as a natural preservative for red snapper with the highest concentration of protein.
Potential of Crude Protease of Bacillus Sp. HSFI-9 ss Anticoagulant Agent and Meat Tenderizer Izza, Naimatul; Saptaningtyas, Ragil; Stalis Norma Ethica; Kartika, Aprilia Indra
Jurnal Kesehatan Prima Vol. 19 No. 2 (2025): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v19i2.1663

Abstract

Some proteases act as thrombolytic enzymes to destroy the abnormal blood clot in the body called a thrombus, a leading cause of mortality associated with cardiovascular disease (CVD). In addition, the enzyme may also hydrolyze the meat’s proteins into simple amino acids, causing the meat to become tender. The study's aim was to analyze the potential of the crude protease of Bacillus sp. HSFI-9 is an anticoagulant agent and meat tenderizer. The anticoagulant test of crude protease HSFI-9 on venous blood samples was performed by measuring the clotting time compared to control (10% EDTA) by the Lee-White method. Protein profiles of beef, chicken, and tuna were analyzed before and after immersion in the crude protease of Bacillus sp. HSFI-9 in concentration of 30% v/v for 3 hours based on Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. The anticoagulant test screening showed that the crude protease of Bacillus sp.  HSFI-9 was able to prolong the blood clotting time even though its anticoagulant activity was still less than that of 10% EDTA (a commercial anticoagulant agent used as a positive control). The SDS-PAGE results indicated that soaked beef and chicken using crude protease from Bacillus sp. HSFI-9 could hydrolyze the meats’ proteins by denaturing them into smaller molecules, indicated by the appearance of new minor protein bands and the disappearance of major ones. It can be concluded that the crude protease of Bacillus sp. has potential as an anticoagulant agent and a meat tenderizer.
Perbandingan Kadar Tumor Necrosis Factor Alpha (TNF- α) Pada Tukang Parkir 1-3 Tahun dan >3 Tahun Dengan Metode ELISA Hidayat, Ahmad; Kartika, Aprilia Indra; Fitria, Meutia Srikandi
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 2 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i2.237

Abstract

Polusi udara terdiri atas campuran substansi-substansi kimia dalam bentuk gas dan zat-zat radikal bebas. Radikal bebas yang masuk ke dalam saluran napas secara langsung dapat mengganggu mekanisme pertahanan antioksidan tubuh serta dapat mengakibatkan terjadinya kerusakan atau oksidasi pada lipid, protein dan DNA pada sel. Radikal bebas yang masuk akan di detoksifikasi oleh sel makrofag, neutrofil, eosinofil dan sel epitel. Hasil dari detoksifikasi radikal bebas oleh sistem pertahanan tubuh berupa Reactive Oxygen Species (ROS), yang kemudian memicu pelepasan sitokin-sitokin proinflmasi salah satunya ialah Tumor Necrosis Factor Alpha (TNF-?). Tujuan penelitian ini untuk mengetahui perbandingan kadar TNF-? pada tukang parkir 1-3 tahun dan >3 tahun menggunakan metode ELISA, sampel yang digunakan adalah tukang parkir dengan lama kerja 1-3 tahun sebanyak 10 serum dan >3 tahun sebanyak 10 serum. Hasil penelitian menunjukan bahwa kadar rata-rata TNF-? pada sampel serum tukang parkir dengan lama kerja >3 tahun (1995.0565 pg/ml) lebih tinggi dibandingkan dengan masa kerja 1-3 tahun (1962.7485 pg/ml) sehingga lebih beresiko terkena penyakit paru obstruktif kronis, kanker paru, penyakit jantung koroner, aterosklerosis dan penyakit kardiovaskuler.
Antibakteri Kombinasi Ekstrak Binahong dengan Antibiotik Meropenem terhadap MDRPA Penyebab HAIs Rahmasari, Galuh Dwi Mustika; Noer, Anashirul Ishlah; Wabula, Mega Berkah Mustika; Sadiq, Alfian La Ode; Kartika, Aprilia Indra
Prosiding University Research Colloquium Proceeding of The 10th University Research Colloquium 2019: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Infeksi nosokomial atau Healthcrae Assicated Infection(HAIs) banyak yang disebabkan oleh bakteri patogen nosocomial Pseudomonas aeruginosa. Angka infeksi nosocomial yang disebabkan oleh bakteri ini 10%-20% pada unit ICU, biasanya terjadi pada pasien septicemia, sistik fibrosis, luka bakar dan luka infeksi. Infeksi Pseudomonas aeruginosa dapat diobati dengan antibiotic yaitu dengan antibiotic meropenem. Tanaman Binahong (Anredera cordifolia(Ten) Steeins) adalah salah satu tanaman yang secara empiris digunakan sebagai obat antibakteri. Penelitian ini bertujuan untuk mengkombinasikan antara antibiotic meropenem dan ekstrak daun binahong dalam menghambat pertumbuhan bakteri Pseudomonas aeruginosa. Metode penelitian ini menggunakan desain eksperimental dan metode uji daya hambat bakteri menggunakan uji difusi agar. Hasil uji kombinasi ekstrak binahong pada konsentrasi 20% memiliki zona hambat dengan rata-rata 39mm sedangkan perlakuan antibiotik tanpa kombinasi memiliki rata-rata zona hambat 36mm. Kombinasi ekstrak binahong konsentrasii 20% dengan antibiotik meropenem dapat menghambat bakteri Pseudomonas aeruginosa dengan daya hambat lebih besar dari penggunaan antibiotic tanpa kombinasi ekstrak binahong.
Single Nucleotide Variation in the Promoter Region of the APOA1 Gene as a Candidate Biomarker for Dyslipidemia Rahajoe, Toeti; Nugraheni, Kartika; Pinda Ayu Widiyani; Asihanto, Balqis Aureola Putri; Kartika, Aprilia Indra
JURNAL INFO KESEHATAN Vol 21 No 4 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss4.1222

Abstract

Dyslipidemia is a lipid profile abnormality that can escalate the risk of cardiovascular disease. The rate of cardiovascular events in Indonesia is very high. One of the causes of dyslipidemia is due to polymorphisms in genes associated with lipid metabolism. The APOA-1 gene encodes the APOA-1 protein which functions to regulate HDL protein synthesis. The objective of this study is to determine APOA1 gene polymorphisms in patients with dyslipidemia. Samples of healthy controls and dyslipidemia patients were used in this investigation. The lipid profiles of the patients and normal controls are determined at the onset of the study. Following DNA extraction, the APOA-1 gene was amplified and sequenced using the serum sample. MEGA X and BLAST were used to analyze the sequencing results. The study's findings demonstrated that the APOA-1 gene length in samples of dyslipidemic patients and normal controls was 433 bp. While the normal control samples have the same sequence as the database, the dyslipidemic patient samples have an APOA-1 gene polymorphism in the promoter region. APOA-1 gene polymorphism results in disturbances in lipid profiles, particularly HDL which is at risk of developing dyslipidemia. The APOA-1 gene has the potential to be developed as a biomarker for diagnosing dyslipidemia involving a larger number of samples.
Co-Authors Ahmad Hidayat Asihanto, Balqis Aureola Putri Budi Santosa Budi Santosa Dafip, Muchamad Dewi Mustikaningtyas ERMA WIDYA LESTARI, ERMA Fandhi Adi Wardoyo Fitria, Meutia Srikandi Gasem, Muhammad Husein Herlisa Anggraini Izza, Naimatul Kakiay, Grevanmoy Febriani Kartika Nugraheni Krispinus Kedati Pukan La’lang, Marselaonety Meliana, Monica Nikmah, Khofifatun Noer, Anashirul Ishlah Oktaviola, Vanny Pinda Ayu Widiyani Ragil Saptaningtyas, Ragil Rahajoe, Toeti Rahmasari, Galuh Dwi Mustika Redistia, P Shinta Reki, Winarto Retno Sri Iswari Sadiq, Alfian La Ode Sri Darmawati Stalis Norma Ethica Telleng, Prisca Audra Wabula, Mega Berkah Mustika