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Yoga Aji Handoko
Universitas Kristen Satya Wacana
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Journal : Jurnal Ilmiah Pertanian

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Isolasi, karakterisasi dan uji stabilitas pH bakteriofag Xanthomonas oryzae dari area persawahan Desy Mar’atul Fadlilah; Andree Wijaya Setiawan; Yoga Aji Handoko
Jurnal Ilmiah Pertanian Vol. 19 No. 2 (2022): Jurnal Ilmiah Pertanian
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31849/jip.v19i2.9417

Abstract

The efforts to increase paddy yields are integrated with disease control. Bacterial Leaf Blight (BLB) is a disease that attacks paddy plants caused by Xanthomonas oryzae bacteria. So far, the control carried out by farmers is by spraying bactericides, planting disease-resistant varieties, and rotating plants that are not pathogenic hosts. However, this method of controlling has not obtained satisfactory results. Therefore, we need an alternative option in overcoming BLB disease, namely using bacteriophages as biocontrol agents in infecting Xanthomonas oryzae bacteria. This study explored the potential of bacteriophages isolated from soil samples near paddy plants' root areas and tested their particle's stability. The stages of experiments were soil sampling, isolation, purification, propagation, and pH stability examination. The results showed that the Xanthomonas oryzae bacteriophages had been isolated, purified, and propagated with the coding Kalibening 1 (XB1), Kalibening (XB2), and Kalibening 3 (XB3). The results of the pH stability test on the three samples also showed that the Xanthomonas oryzae bacteriophages experienced particle instability and the titer tended to drop in the pH range of 3-5, while the Xanthomonas oryzae bacteriophages tended to be stable at neutral and alkaline pH 7-11, particularly XB1 and XB2 bacteriophages.
Co-Authors Adi Nugroho Adminarwati Hulu Adminarwati Hulu Agus, Yohanes Hendro Alfred Jansen Sutrisno Alvira Tuttan Dwi Maharani Andree Wijaya Setiawan Andrianto, Kurniawan Apria Ajeng Winanti Arita Putri Handayani Arita Putri Handayani Bayu Nuswantara Bellarose Novelia Kristina Pandeirot Bellarose Novelia Pandeirot Bistok Hasiholan Simanjuntak Brian Jordan Zendrato Briliani Ayu Arianita Chelsy Natalia Putirulan Christhoperus William Vincent Sucipto Christiani Yasmine Damara Dinda Nirmalasari Zebua David Richard Hendarto Deshinta Tri Murty Archery Desy Mar’atul Fadlilah Dwi Septian, Bagas Eko Wahyu Widiyatmoko Eko Wahyu Widiyatmoko Elen, Sisilia Elisa Esterelita Elvi Fitriani Simarmata Erlitha Rahmawati Faisalma, Mohamad Wega Gabriella Desnata Yoan Anarki Gideon Febby Prima Andhika Gilang Andi Nugroho Hendrik Johannes Nadapdap Hildegardis Vania Santika Hulu, Adminarwati Indrabayu Indriatmoko Indriatmoko Ivan Tjahja Pranata Jatiningrum, Setia Humani Jayanti, Malta Puspita Jennifer Larisa Liem Johan Jimmy Carter Tambotoh Krisdania Agaperesa Lasmono Tri Sunaryanto Liem, Jennifer Larisa Liska Simamora Lois Hintanara Shine Tanggara Manao, Nancy Felicia Maria Maria Maria Maria Maria Maria Marina Herawati Megawati Haloho Murtiwulandari Murtiwulandari Nokabun, Karolus Yubel Novani Wahyu Khristanti Novia Dewi Permatasari Nugraheni Widyawati Olsen O Pramana Panadi, Danendra Guido Pandeirot, Bellarose Novelia Kristina Paulya Eltje Leihitu Peli Rodo Pranata, Ivan Tjahja Pratiwi, Vera Handi Putra, Imanuel Trisya Putri, Christina Ari Pramono Rannes Ran Nes Rendha Kinasih Rony, Zahara Tussoleha Shinta Sugiarti Simanjuntak, istok Hasiholan Sisilia Elen Subagyo, Ni Jeni Fanira Sucipto, Christhoperus Vincent Sugiarti, Shinta Suprihati Suprihati Surya Seno Pamungkas Teresha Elena Permata Theresa Dwi Kurnia Tinjung Mary Prihtanti Vania Putri Santoso Velian Sandy Wardana Wamilia Yulianingsih Wamilia Yulianingsih Widhi Handayani Yasmine, Christiani Yayi Suryo Prabandari Yetero Hendikus Hulu Yohanes Hendro Agus Yuli Kristiyanto Yulianingsih, Wamilia Yuliawati Yuliawati Yulius Adi Kristiawan