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All Journal Alchemy : Journal of Chemistry Current Biochemistry The Journal of Pure and Applied Chemistry Research JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Chimica et Natura Acta QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Kartika Kimia Journal of Science Innovare EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Abdimas Kartika Wijayakusuma Jurnal Sains dan Teknologi International Journal of Ethno-Sciences and Education Research Helium: Journal of Science and Applied Chemistry Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Advances In Social Humanities Research Jurnal Pengabdian Masyarakat Inovatif Jurnal Pengabdian Masyarakat Inovatif
Siti Warnasih
Universitas Pakuan
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Journal : Journal of Science Innovare

 1 
PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw) Warnasih, Siti; Hasanah, Uswatun
Journal of Science Innovare Vol 1, No 02 (2018): Journal of Science Innovare, September 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.825 KB) | DOI: 10.33751/jsi.v1i02.1000

Abstract

One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and  total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 °C, and kluwek extract unstable when contact with 1% H2O2, UV light, and if kept at cold temperature (10 ˚C).
PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw) Siti Warnasih; Uswatun Hasanah
Journal of Science Innovare Vol 1, No 02 (2018): Journal of Science Innovare, September 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (415.825 KB) | DOI: 10.33751/jsi.v1i02.1000

Abstract

One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 C, and kluwek extract unstable when contact with 1% H2O2, UV light, and if kept at cold temperature (10 ?C).
DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING UV-VIS SPECTROPHOTOMETER Uswatun Hasanah; Ade Heri Mulyati; Sutanto .; Diana Widiastuti; Siti Warnasih; Yulian Syahputri; Tri Panji
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3007

Abstract

The COD (Chemical Oxygen Demand) analysis method based on the Indonesian National Standard (SNI) is a revision of SNI 06-6989.2-2004, Water and wastewater - Part 2: Method of testing for chemical oxygen demand (COD) with closed reflux spectrophotometrically. This SNI uses references from international standard methods, namely Standard Methods for the Examination of Water and Wastewater, 21st Edition, editor of LS Clesceri, AE Greenberg, AD Eaton, APHA, AWWA and WEF, Washington DC, 2005, Methods 5220 D (Closed Reflux, Colorimetric Methods). The purpose of this study was to validate the total method for testing chemical oxygen demand (COD) in water and wastewater with the reduction of Cr2O7 2- spectrophotometrically in the range of COD values of 100 mg / L to 900 mg / L measurements were made at a wavelength of 600 nm. and COD values less than or equal to 90 mg / L measurements were made at a wavelength of 420 nm. The results showed that the method SNI 06-6898.2- 2009  has good validation results including the r results obtained from the calibration curve equation of 0.998, a precision of 1.82%, an accuracy of 98.25% and LOD and LOQ of 12.27 mg / L and 25, 61 mg / L.
DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING SPECTRODIRECT Ade Heri Mulyati; Leny Heliawati; Sutanto .; Uswatun Hasanah; Diana Widiastuti; Siti Warnasih; Yulian Syahputri
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3006

Abstract

Increased human activities will cause various problems, one of which is water pollution in water sources because it receives a pollution load that exceeds its carrying capacity. In the context of environmental conservation, the government has set quality standards for liquid waste produced by various industries and other activities in a Decree of the State Minister for the Environment. COD (Chemical Oxygen Demand) is one of the most important parameters in wastewater quality standards. This study aims to validate the COD analysis method which refers to COD Thermoreactor System (LOVIBOND brand) at Pakuan University Service Laboratory, to ensure the validity of COD analysis results at Pakuan University Service Laboratory. Spectrodirect COD analysis method provides results that meet the requirements of accuracy, precision, LOD, and LOQ which is quite good. The results of the Coefficient of Variation give good results with a value of 1.8841% indicating the accuracy of the analysis method is good. The accuracy parameter also gives good results and meets the requirements with a% recovery value of 98.70351%. Overall, it can be stated that the spectrodirect analysis method tested is an accurate, precise, simple, and economical method for determining COD levels
DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING SPECTRODIRECT Ade Heri Mulyati; Leny Heliawati; Sutanto .; Uswatun Hasanah; Diana Widiastuti; Siti Warnasih; Yulian Syahputri
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare, Volume 03 Number 02 2020
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3006

Abstract

Increased human activities will cause various problems, one of which is water pollution in water sources because it receives a pollution load that exceeds its carrying capacity. In the context of environmental conservation, the government has set quality standards for liquid waste produced by various industries and other activities in a Decree of the State Minister for the Environment. COD (Chemical Oxygen Demand) is one of the most important parameters in wastewater quality standards. This study aims to validate the COD analysis method which refers to COD Thermoreactor System (LOVIBOND brand) at Pakuan University Service Laboratory, to ensure the validity of COD analysis results at Pakuan University Service Laboratory. Spectrodirect COD analysis method provides results that meet the requirements of accuracy, precision, LOD, and LOQ which is quite good. The results of the Coefficient of Variation give good results with a value of 1.8841% indicating the accuracy of the analysis method is good. The accuracy parameter also gives good results and meets the requirements with a% recovery value of 98.70351%. Overall, it can be stated that the spectrodirect analysis method tested is an accurate, precise, simple, and economical method for determining COD levels
PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw) Siti Warnasih; Uswatun Hasanah
Journal of Science Innovare Vol 1, No 2 (2018): Journal of Science Innovare, Volume 01 Number 02 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v1i02.1000

Abstract

One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and  total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 °C, and kluwek extract unstable when contact with 1% H2O2, UV light, and if kept at cold temperature (10 ˚C).
DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING UV-VIS SPECTROPHOTOMETER Uswatun Hasanah; Ade Heri Mulyati; Sutanto .; Diana Widiastuti; Siti Warnasih; Yulian Syahputri; Tri Panji
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare, Volume 03 Number 02 2020
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3007

Abstract

The COD (Chemical Oxygen Demand) analysis method based on the Indonesian National Standard (SNI) is a revision of SNI 06-6989.2-2004, Water and wastewater - Part 2: Method of testing for chemical oxygen demand (COD) with closed reflux spectrophotometrically. This SNI uses references from international standard methods, namely Standard Methods for the Examination of Water and Wastewater, 21st Edition, editor of LS Clesceri, AE Greenberg, AD Eaton, APHA, AWWA and WEF, Washington DC, 2005, Methods 5220 D (Closed Reflux, Colorimetric Methods). The purpose of this study was to validate the total method for testing chemical oxygen demand (COD) in water and wastewater with the reduction of Cr2O7 2- spectrophotometrically in the range of COD values of 100 mg / L to 900 mg / L measurements were made at a wavelength of 600 nm. and COD values less than or equal to 90 mg / L measurements were made at a wavelength of 420 nm. The results showed that the method SNI 06-6898.2- 2009  has good validation results including the r results obtained from the calibration curve equation of 0.998, a precision of 1.82%, an accuracy of 98.25% and LOD and LOQ of 12.27 mg / L and 25, 61 mg / L.
Co-Authors Achmad, Dinar Munggaran Ade Heri Mulyati Ade Heri Mulyati, Ade Heri Agustine, Dine Alawiyah, Hanis Amelia, Nisa Anak Agung Istri Sri Wiadnyani Ani Iryani Anna Muthia Soliha Artika, I. M Deden Ardiansyah Dhiandani, Sri Boedi Diana Widiastuti Dianda Namira Azzahra Effendi, Herlinda Eka Herlina Elly Sukmanasa Eneng Tita Tosida Faliha Arinda Lestari Farid Ramadhan Fredi Andria Gita Syahputra Gymnasti, Alista Dwis I. M Artika Indriyani, Silvia Intan Wulandari Irma Anggraeni Junita Darmawarni Kholisoh, Siti LAKSMI AMBARSARI Leny Heliawati Linda Jati Kusumawardani Lisa Giovanny Marom, Muhamad Fathul Mastri Juniarto Novi Fajar Utami Nurmayani, Reni Nurul Marfira Prihastuti Harsani Purwantiningsih Sugita R. T. Sasmono Sabania, Ikrimah Nur Sadjarwa, Boedi Samsudin Samsudin Sasmono, R. T. Simalango, Siska Juliani Sinaga, Siska Elisahbet Siti Fatimah Siti Kholisoh Sofian, Yayan Susanto Susanto Sutanto Sutanto . Sutanto Sutanto Sutanto Sutanto Sutoyo, Muhammad Rayhan Tamimah Shafwatul Ishlah Tri Panji Triastinurmiatiningsih, Triastinurmiatiningsih Uswatun Hasanah USWATUN HASANAH Uswatun Hasanah W Yulia Yuli Wahyuni Yulia, W Yulian Syahputri Zahra, Mutiara Zuniar Subastian