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All Journal Alchemy : Journal of Chemistry Current Biochemistry The Journal of Pure and Applied Chemistry Research JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Chimica et Natura Acta QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Kartika Kimia Journal of Science Innovare EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Abdimas Kartika Wijayakusuma International Journal of Ethno-Sciences and Education Research Helium: Journal of Science and Applied Chemistry Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Advances In Social Humanities Research Jurnal Pengabdian Masyarakat Inovatif Jurnal Pengabdian Masyarakat Inovatif
Siti Warnasih
Universitas Pakuan
Religion Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Transportation Other

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Journal : Current Biochemistry

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Induksi Ekspresi Gen Sitokin/Kemokin pada Sel Makrofag Manusia yang Dipapar Virus Dengue Isolat Indonesia Siti Warnasih
Current Biochemistry Vol. 1 No. 3 (2014)
Publisher : IPB University

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Abstract

Dengue is one of the world's most important arbovirus disease. Dengue pathogenesis has not been yet fully understood. It has been reported that there is involvement of the host immune factors and viral factors. Several studies have shown that concentrations of cytokines/chemokines on blood are significantly increased during infection and viral factors are also involved in disease severity. Therefore, characterization of host gene expression profiles in response to dengue virus infection of different serotypes could provide input for understanding the pathogenesis of dengue. The purpose of this research was to determine expression profiles of the genes (mRNA) of cytokines/chemokines as immune response that are released by monocyte derived macrophages (MDM) cells (host gene) exposed dengue viruses. Four dengue serotypes of Indonesia isolate were used in this study. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from blood cells of healthy donors by Ficoll gradient centrifugation techniques and then differentiated into MDM cells. Quantitative real time RT-PCR was used to quantify expression levels of cytokine/chemokine-encoding genes from MDM cells infected dengue. Four cytokine/chemokine-encoding genes i.e IP-10, MCP-1, IL-10, and MIP-1β known involved in dengue pathogenesis. Measurement of the expression levels of cytokines/ chemokines showed that the dengue virus of serotypes DENV-1 and DENV-3 caused an increase in the expression of genes encoding cytokine IL-10 and chemokine IP-10 is higher than other serotypes. Further research is needed to better determine the pathogenesis of dengue disease.
Potency of Ethanol Extracts of Palm Seeds (Phoenix dactylifera L.) as Antidiabetic with Inhibition Kinetics Parameter Lisa Giovanny; Faliha Arinda Lestari; Nurul Marfira; Laksmi Ambarsari; Siti Warnasih
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.1

Abstract

Dates seeds are one of the byproducts of the date palm juice industry which has the potential for further processing. Dates seeds contain active compounds that are known to have ability to inhibit αglucosidase. This study aims to measure the activity of 70% ethanol extract and the fraction of date palm seeds with a mechanism in inhibiting αglucosidase. The measurement of inhibitory activity was carried out by extracting date palm seeds using 70% water and ethanol. The extraction product is then fractionated with n-hexane, ethyl acetate, n-butanol, and water. Then the total phenolic and flavonoid calculations were calculated for each extract and fraction. Measurement of α-glucosidase inhibition activity was measured based on reaction of substrate to enzyme by the addition of inhibitors. The results showed that the ethanolextract had the highest total phenolic and flavonoid, which were 76.86 mg GAE / g and 21.19 mg QE / g, respectively. n-hexane fraction have the best inhibitory activity seen from the IC50 value, which is 12.69 mg/L. The kinetics of inhibition of 70% ethanol extract of date palm seeds are mixed inhibition. Keywords: α-glucosidase, date seeds, flavonoids, kinetic inhibition, phenolics
Co-Authors Achmad, Dinar Munggaran Ade Heri Mulyati Ade Heri Mulyati, Ade Heri Agustine, Dine Alawiyah, Hanis Amelia, Nisa Anak Agung Istri Sri Wiadnyani Ani Iryani Anna Muthia Soliha Artika, I. M Deden Ardiansyah Dhiandani, Sri Boedi Diana Widiastuti Dianda Namira Azzahra Effendi, Herlinda Eka Herlina Elly Sukmanasa Eneng Tita Tosida Faliha Arinda Lestari Fredi Andria Gita Syahputra Gymnasti, Alista Dwis I. M Artika Indriyani, Silvia Intan Wulandari Irma Anggraeni Kholisoh, Siti LAKSMI AMBARSARI Leny Heliawati Linda Jati Kusumawardani Lisa Giovanny Marom, Muhamad Fathul Mastri Juniarto Novi Fajar Utami Nurmayani, Reni Nurul Marfira Prihastuti Harsani Purwantiningsih Sugita R. T. Sasmono Sabania, Ikrimah Nur Sadjarwa, Boedi Samsudin Samsudin Sasmono, R. T. Simalango, Siska Juliani Sinaga, Siska Elisahbet Siti Fatimah Siti Kholisoh Sofian, Yayan Sutanto Sutanto . Sutanto Sutanto Sutanto Sutanto Sutoyo, Muhammad Rayhan Tamimah Shafwatul Ishlah Tri Panji Triastinurmiatiningsih, Triastinurmiatiningsih Uswatun Hasanah Uswatun Hasanah USWATUN HASANAH W Yulia Yuli Wahyuni Yulia, W Yulian Syahputri Zahra, Mutiara Zuniar Subastian