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All Journal Biotropika Jurnal Sumberdaya Hayati Jurnal Ilmiah Biosaintropis (Bioscience-Tropic) Bioma : Jurnal Biologi Indonesia JMM (Jurnal Masyarakat Mandiri) Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Bubungan Tinggi: Jurnal Pengabdian Masyarakat Konservasi Hayati Jurnal Kajian Ilmiah Jurnal Mandala Pengabdian Masyarakat
Desimaria Panjaitan
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Palangka Raya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Public Health Social Sciences Other

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Inventarisasi Anggrek (Orchidaceae) di Kawasan Taman Wisata Alam Bukit Tangkiling Kalimantan Tengah Erna Wati; Sunariyati, Siti; Panjaitan, Desimaria
Konservasi Hayati Vol 20 No 1 (2024): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v20i1.29812

Abstract

Orchid (Orchidaceae) is one among the plants that grow in Taman Wisata Alam (TWA) Bukit Tangkiling, Central Kalimantan. The lack of information about orchid species in that area makes an inventory crucial. The purpose of this study was to inventory the species of orchids in the TWA Bukit Tangkiling area, as well as to learn about the environmental characteristics and distribution patterns of these plants. The research was conducted from October 2022 to March 2023. This research used the exploratory method and the sample was taken using the purposive sampling technique. Seven orchid species were identified as a result of this study: Acriopsis sp., Arachnis sp., Cymbidium finlaysonianum, Dendrobium crumenatum, Bulbophyllum purpurascens, Bromheadia finlaysoniana, and Dipodium pictum. Five species grow on plant stems (epiphytic), while two species grow on soil (terrestrial). Since each species was discovered less than 100 meters above sea level, they were all categorized as lowland orchid species. Dendrobium crumenatum is distributed randomly. The distribution of Bromheadia finlaysoniana, Bulbophyllum purpurascens, and Cyimbidium finlaysonianum is clustered. While the distribution pattern of the rest could not be determined because only one individual was found.
Peningkatan Keterampilan Guru-Guru Biologi SMA di Palangka Raya Dalam Pelatihan Pembuatan Preparat Permanen Jaringan Tumbuhan Sebagai Media Pembelajaran Lestari, Ria Windi; Aziz, Fadhila; Adelia, Kadek Ayu Cintya; Panjaitan, Desimaria; Septya, Lia
Jurnal Mandala Pengabdian Masyarakat Vol. 6 No. 1 (2025): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpm.v6i1.593

Abstract

Praktikum mikroskopis pada pelajaran biologi di sekolah sering kali menghadapi kendala terkait dengan ketersediaan preparat yang berkualitas. Untuk itu, pelatihan pembuatan preparat permanen jaringan tumbuhan bertujuan untuk meningkatkan keterampilan guru dalam membuat preparat mikroskopis menggunakan teknik parafin. Kegiatan ini diselenggarakan di Gedung Pusat Pengembangan IPTEK dan Inovasi Gambut (PPIIG), Universitas Palangka Raya, dengan diikuti oleh sembilan orang guru biologi dari sekolah mitra sasaran. Pelatihan dimulai dengan pemaparan materi mengenai mikroteknik tumbuhan, yang mencakup langkah-langkah pembuatan preparat, mulai dari fiksasi, dehidrasi, penyayatan, pewarnaan, hingga pemasangan preparat. Para peserta juga diberikan kesempatan untuk praktik langsung pembuatan preparat segar dan awetan, serta teknik pewarnaan dan mounting menggunakan mikrotom dan safranin. Kuesioner pra dan pasca pelatihan digunakan untuk mengevaluasi pemahaman dan keterampilan peserta, yang menunjukkan peningkatan signifikan setelah kegiatan. Sebelum pelatihan, tidak ada peserta yang memahami atau memiliki keterampilan dalam pembuatan preparat permanen jaringan tumbuhan. Namun, setelah pelatihan, seluruh peserta berhasil menguasai teknik tersebut. Evaluasi ini menunjukkan bahwa pelatihan ini efektif dalam meningkatkan kompetensi praktis guru, yang diharapkan dapat meningkatkan kualitas praktikum biologi di sekolah. Ke depan, pelatihan ini memiliki potensi untuk dikembangkan lebih lanjut dengan topik-topik tambahan seperti perawatan mikroskop dan teknik kultur jaringan.
ISOLASI BAKTERI ENDOFIT BAWANG DAYAK (Eleutherine bulbosa) DAN UJI ANTAGONISME TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus Frans Naibaho; Ebrry Dwi Putra; Liswara Neneng; Desimaria Panjaitan
Bioma Vol. 19 No. 1 (2023): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma19(1).5

Abstract

Antibiotic resistance is one of the main challenges faced by the health world today. Therefore, various efforts are being made to find new alternative antibiotics from various sources. Endophytic bacteria from medicinal plants are thought to have an antibacterial activity that can potentially inhibit pathogenic bacteria growth. This study aimed to isolate and characterize endophytic bacteria from the Dayak onion plant and determine their potential to inhibit the growth of two pathogenic bacteria Escherichia coli and Staphylococcus aureus. Bacterial isolation was carried out using the surface sterilization method and grown on nutrient agar media. The endophytic bacteria isolates obtained were then characterized macroscopically and microscopically. The agar plug diffusion method carried out the antagonism test against pathogenic bacteria. The result showed that 40 isolates of endophytic bacteria were successfully isolated from the Dayak onion plant's leaves, bulbs, and roots. Based on the antagonism test, 3 isolates were able to inhibit E.coli while 8 isolates were able to inhibit S. aureus. Three isolates (CED3, CED4, and CEU6) were able to inhibit both pathogenic bacteria, and the highest activity was shown by CED3 isolates.
Antimicrobial and Antioxidant Activities of Some Xylariaceous Fungi Panjaitan, Desimaria; Noraini; Norwahyunie; Fastanti, Fandri Sofiana; Naibaho, Frans Grovy
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.01

Abstract

The Xylariaceae family comprises cosmopolitan ascomycetous fungi that predominantly live as saprobes, contributing ecologically and forming interactions with various organisms. Due to their cultivability under laboratory conditions and potential to produce bioactive metabolites, members of Xylariaceae are increasingly explored for bioprospecting. This study aimed to evaluate the antimicrobial and antioxidant activities of mycelial extracts from Xylariaceae isolates collected from Central Kalimantan, Indonesia, as an initial step in exploring their bioprospective traits. Fungal specimens obtained from forest exploration were cultured in vitro to obtain axenic cultures. Liquid-state fermentation was performed to induce metabolite secretion, and the biomass was extracted (macerated) using ethyl acetate (EtOAc) and methanol (MeOH). The antimicrobial activity was tested against Bacillus cereus, Staphylococcus aureus, and Candida albicans using the disk diffusion method. Antioxidant capacity was assessed via the DPPH radical scavenging assay and expressed as IC50 values. Morphological examination of six isolates (DP61–DP66) revealed characteristic features of Xylariaceae, such as upright stromata, clavate dark structures, and smooth to punctate surfaces, while their mycelia appeared white and unpigmented. Ethyl acetate extracts generally exhibited broader antibacterial effects than methanolic extracts. The EtOAc-DP62 extract exhibited strong antibacterial activity, with inhibition zones of 13.3 mm and 13.2 mm against B. cereus and S. aureus, respectively. MeOH-DP66 demonstrated the strongest antifungal activity, with an inhibition zone of 21.7 mm. DP62 also exhibited potent antioxidant activity (IC50 = 3.44 µg/mL), surpassing ascorbic acid (IC50 = 28.32 µg/mL). Molecular identification based on ITS-rDNA sequences revealed DP62 as Nectriopsis epimycota (Bionectriaceae) and DP66 as Xylaria plebeja (Xylariaceae). Interestingly, DP62 may represent an endohyphal fungus initially associated with a xylariaceous fruiting body. These findings highlight the bioprospective value of these fungal isolates as promising antimicrobial and antioxidant-producing agents.
Co-Authors Agus Lasono Anita Maulina Ayuchecaria, Noverda Decenly Decenly Dian Mutiasari, Dian Ebrry Dwi Putra Elvina Damayanti erna wati Erwin Prasetya Toepak Fadhila Aziz Fandi Tuju Faridah Tsuraya Fastanti, Fandri Sofiana Febri Nur Ngazizah Frans Grovy Naibaho Frethernety, Agnes Hanasia Ika Oktavia Wulandari Ivan Permana Putra Kadek Ayu Cintya Adelia Krestina, Widya Liswara Neneng Malik, Handayani Mirnawati Dewi Naibaho, Frans Noraini Norwahyunie Rahman, Sudarman Rahmawati, Maynissa Retno Agnestisia Ria Windi Lestari Rizko Hadi Rovik, Anwar Santi Dwi Febiolasari Sari Namarito Simamarta Satriani, Inriyati Septya, Lia Siti Sunariyati Stevin Carolius Angga Turnip, Oktaviani Naulita Vinsen Willi Wardhana Zulviana