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Keanekaragaman Jamur Makroskopis di Kawasan Hutan Universitas Palangka Raya Kalimantan Tengah Panjaitan, Desimaria; Wardhana, Vinsen Willi; Febiolasari, Santi Dwi
Jurnal Kajian Ilmiah Vol. 22 No. 2 (2022): May 2022
Publisher : Lembaga Penelitian, Pengabdian Kepada Masyarakat dan Publikasi (LPPMP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31599/hazg7p13

Abstract

Macroscopic fungi comprise a very diverse group of heterotrophic and mostly saprophytic organisms. This research purpose was to identify the diversity of macroscopic fungi from the forest of University of Palangka Raya. The fungi samples were collected from May until June 2021 using purposive sampling method. The samples were identified based on macroscopic characters such as the form of fruiting bodies, lamellae, cap shape and colours. There were 18 species of Basidiomycota which consist of 9 famili and 12 genera. The species were identified as Agrocybe sp., Auricularia sp.1, Auricularia sp.2, Collybia sp., Dacryopinax spathularia, Ganoderma sp.1, Ganoderma sp.2, Ganoderma sp.3, Hygrocybe sp., Marasmiellus sp., Mycena sp.1, Mycena sp.2, Pycnoporus sanguineus, Schizophyllum commune, Trametes sp.1, Trametes sp.2, Trametes sp.3, and Tyromyces sp. Descriptions of each species were provided.
Keanekaragaman Jamur Makroskopis di Hutan Gambut Arboretum Nyaru Menteng Kalimantan Tengah Desimaria Panjaitan; Frans Grovy Naibaho; Retno Agnestisia; Ivan Permana Putra
Konservasi Hayati Vol 19 No 2 (2023): OKTOBER
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v19i2.29404

Abstract

Information on macroscopic fungi from Central Kalimantan is still limited compared to other regions in Indonesia. Arboretum Nyaru Menteng, a conservation area located in Central Kalimantan, poses many macrofungi due to its favorable environmental conditions that support the growth of various types of macrofungi. However, the diversity of macroscopic fungi from Arboretum Nyaru Menteng has never been reported. Thus, the current works aimed to provide initial information on the diversity of macroscopic fungi in this conservation area. The exploration was done using the opportunistic sampling method. Fruiting bodies were documented, collected, and identified based on macroscopic characteristics. Four environmental microclimate variables were measured in four random sampling locations: temperature, light intensity, air humidity, and soil acidity. The results confirmed that there were 27 species of macrofungi belonging to 18 genera and 14 families. Polyporaceae was the most dominant family found in this research. Most fungi grew on a dead log while the others were recognized as mycorrhizal fungi and parasites on plants. The environmental measurements showed that Arboretum Nyaru Menteng has an optimal habitat for recorded fungi growth. The record of macrofungi diversity in this research contributes to Indonesia mycoflora, particularly from Central Kalimantan.
Inventarisasi Anggrek (Orchidaceae) di Kawasan Taman Wisata Alam Bukit Tangkiling Kalimantan Tengah Erna Wati; Sunariyati, Siti; Panjaitan, Desimaria
Konservasi Hayati Vol 20 No 1 (2024): APRIL
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/hayati.v20i1.29812

Abstract

Orchid (Orchidaceae) is one among the plants that grow in Taman Wisata Alam (TWA) Bukit Tangkiling, Central Kalimantan. The lack of information about orchid species in that area makes an inventory crucial. The purpose of this study was to inventory the species of orchids in the TWA Bukit Tangkiling area, as well as to learn about the environmental characteristics and distribution patterns of these plants. The research was conducted from October 2022 to March 2023. This research used the exploratory method and the sample was taken using the purposive sampling technique. Seven orchid species were identified as a result of this study: Acriopsis sp., Arachnis sp., Cymbidium finlaysonianum, Dendrobium crumenatum, Bulbophyllum purpurascens, Bromheadia finlaysoniana, and Dipodium pictum. Five species grow on plant stems (epiphytic), while two species grow on soil (terrestrial). Since each species was discovered less than 100 meters above sea level, they were all categorized as lowland orchid species. Dendrobium crumenatum is distributed randomly. The distribution of Bromheadia finlaysoniana, Bulbophyllum purpurascens, and Cyimbidium finlaysonianum is clustered. While the distribution pattern of the rest could not be determined because only one individual was found.
Peningkatan Keterampilan Guru-Guru Biologi SMA di Palangka Raya Dalam Pelatihan Pembuatan Preparat Permanen Jaringan Tumbuhan Sebagai Media Pembelajaran Lestari, Ria Windi; Aziz, Fadhila; Adelia, Kadek Ayu Cintya; Panjaitan, Desimaria; Septya, Lia
Jurnal Mandala Pengabdian Masyarakat Vol. 6 No. 1 (2025): Jurnal Mandala Pengabdian Masyarakat
Publisher : Progran Studi Farmasi Universitas Mandala Waluya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35311/jmpm.v6i1.593

Abstract

Praktikum mikroskopis pada pelajaran biologi di sekolah sering kali menghadapi kendala terkait dengan ketersediaan preparat yang berkualitas. Untuk itu, pelatihan pembuatan preparat permanen jaringan tumbuhan bertujuan untuk meningkatkan keterampilan guru dalam membuat preparat mikroskopis menggunakan teknik parafin. Kegiatan ini diselenggarakan di Gedung Pusat Pengembangan IPTEK dan Inovasi Gambut (PPIIG), Universitas Palangka Raya, dengan diikuti oleh sembilan orang guru biologi dari sekolah mitra sasaran. Pelatihan dimulai dengan pemaparan materi mengenai mikroteknik tumbuhan, yang mencakup langkah-langkah pembuatan preparat, mulai dari fiksasi, dehidrasi, penyayatan, pewarnaan, hingga pemasangan preparat. Para peserta juga diberikan kesempatan untuk praktik langsung pembuatan preparat segar dan awetan, serta teknik pewarnaan dan mounting menggunakan mikrotom dan safranin. Kuesioner pra dan pasca pelatihan digunakan untuk mengevaluasi pemahaman dan keterampilan peserta, yang menunjukkan peningkatan signifikan setelah kegiatan. Sebelum pelatihan, tidak ada peserta yang memahami atau memiliki keterampilan dalam pembuatan preparat permanen jaringan tumbuhan. Namun, setelah pelatihan, seluruh peserta berhasil menguasai teknik tersebut. Evaluasi ini menunjukkan bahwa pelatihan ini efektif dalam meningkatkan kompetensi praktis guru, yang diharapkan dapat meningkatkan kualitas praktikum biologi di sekolah. Ke depan, pelatihan ini memiliki potensi untuk dikembangkan lebih lanjut dengan topik-topik tambahan seperti perawatan mikroskop dan teknik kultur jaringan.
ISOLASI BAKTERI ENDOFIT BAWANG DAYAK (Eleutherine bulbosa) DAN UJI ANTAGONISME TERHADAP BAKTERI Escherichia coli DAN Staphylococcus aureus Frans Naibaho; Ebrry Dwi Putra; Liswara Neneng; Desimaria Panjaitan
Bioma Vol. 19 No. 1 (2023): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma19(1).5

Abstract

Antibiotic resistance is one of the main challenges faced by the health world today. Therefore, various efforts are being made to find new alternative antibiotics from various sources. Endophytic bacteria from medicinal plants are thought to have an antibacterial activity that can potentially inhibit pathogenic bacteria growth. This study aimed to isolate and characterize endophytic bacteria from the Dayak onion plant and determine their potential to inhibit the growth of two pathogenic bacteria Escherichia coli and Staphylococcus aureus. Bacterial isolation was carried out using the surface sterilization method and grown on nutrient agar media. The endophytic bacteria isolates obtained were then characterized macroscopically and microscopically. The agar plug diffusion method carried out the antagonism test against pathogenic bacteria. The result showed that 40 isolates of endophytic bacteria were successfully isolated from the Dayak onion plant's leaves, bulbs, and roots. Based on the antagonism test, 3 isolates were able to inhibit E.coli while 8 isolates were able to inhibit S. aureus. Three isolates (CED3, CED4, and CEU6) were able to inhibit both pathogenic bacteria, and the highest activity was shown by CED3 isolates.
Antimicrobial and Antioxidant Activities of Some Xylariaceous Fungi Panjaitan, Desimaria; Noraini; Norwahyunie; Fastanti, Fandri Sofiana; Naibaho, Frans Grovy
Biotropika: Journal of Tropical Biology Vol. 13 No. 3 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2025.013.03.01

Abstract

The Xylariaceae family comprises cosmopolitan ascomycetous fungi that predominantly live as saprobes, contributing ecologically and forming interactions with various organisms. Due to their cultivability under laboratory conditions and potential to produce bioactive metabolites, members of Xylariaceae are increasingly explored for bioprospecting. This study aimed to evaluate the antimicrobial and antioxidant activities of mycelial extracts from Xylariaceae isolates collected from Central Kalimantan, Indonesia, as an initial step in exploring their bioprospective traits. Fungal specimens obtained from forest exploration were cultured in vitro to obtain axenic cultures. Liquid-state fermentation was performed to induce metabolite secretion, and the biomass was extracted (macerated) using ethyl acetate (EtOAc) and methanol (MeOH). The antimicrobial activity was tested against Bacillus cereus, Staphylococcus aureus, and Candida albicans using the disk diffusion method. Antioxidant capacity was assessed via the DPPH radical scavenging assay and expressed as IC50 values. Morphological examination of six isolates (DP61–DP66) revealed characteristic features of Xylariaceae, such as upright stromata, clavate dark structures, and smooth to punctate surfaces, while their mycelia appeared white and unpigmented. Ethyl acetate extracts generally exhibited broader antibacterial effects than methanolic extracts. The EtOAc-DP62 extract exhibited strong antibacterial activity, with inhibition zones of 13.3 mm and 13.2 mm against B. cereus and S. aureus, respectively. MeOH-DP66 demonstrated the strongest antifungal activity, with an inhibition zone of 21.7 mm. DP62 also exhibited potent antioxidant activity (IC50 = 3.44 µg/mL), surpassing ascorbic acid (IC50 = 28.32 µg/mL). Molecular identification based on ITS-rDNA sequences revealed DP62 as Nectriopsis epimycota (Bionectriaceae) and DP66 as Xylaria plebeja (Xylariaceae). Interestingly, DP62 may represent an endohyphal fungus initially associated with a xylariaceous fruiting body. These findings highlight the bioprospective value of these fungal isolates as promising antimicrobial and antioxidant-producing agents.
Studi Dasar Nepenthes spp. di Taman Wisata Alam Bukit Tangkiling, Palangka Raya, Kalimantan Tengah, Indonesia Rizki, Muhammad; Oktafiani, Helen; Panjaitan, Desimaria
Buitenzorg: Journal of Tropical Science Vol 2 No 2 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i2.19

Abstract

Pitcher plants (Nepenthes spp.) are carnivorous plants that flourish in the Bukit Tangkiling Nature Tourism Park (BTNTP), Palangka Raya, Central Kalimantan. Searches of global biodiversity databases and relevant literature revealed no prior records of Nepenthes from this area. This study aimed to document and provide a baseline record of Nepenthes species occurring within BTNTP. The study was conducted from March to May 2022 using exploration and purposive sampling methods to collect plant specimens. Three species, namely Nepenthes gracilis Korth, Nepenthes mirabilis (Lour.) Druce, and Nepenthes rafflesiana Jack, were recorded across various habitats, including scrub forest, heath forest, secondary forest, and peat forest, all below 100 m above sea level. These findings contribute to the species inventory of BTNTP and serve as a scientific reference for future ecological monitoring and management strategies aimed at maintaining habitat quality and supporting long-term Nepenthes conservation efforts in the region. Keywords: habitat, species record, pitcher plants, Nepenthes, Kalimantan