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PEDULI LINGKUNGAN RW 05 DESA CISEMPUR MELALUI PENGELOLAAN SAMPAH Ismail, Mochamad Rudyansyah; Astuty, Sri; Kurnia Agung, Mochamad Untung
Dharmakarya Vol 6, No 4 (2017): Desember
Publisher : Universitas Padjadjaran

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Permasalahan yang terdapat di lingkungan RW 05 Desa Cisempur adalah sampah. Pembungan akhir sampah di desa cisempur yang masih belum jelas dan sering terkendala dengan distribusi sampah. Hal ini perlu adanya pengelolaan yang baik mengenai pembuangan sampah di desa cisempur, terutama RW 05. Pengelolaan yang dilakukan adalah pengelolaan berbasis masyarakat, sehingga masyarakat sadar akan lingkungan yang sehat dan bersih. Penyediaan tempat sampah di sekitar lingkungan RW 05 Desa Cisempur adalah langkah awal dalam usaha penyadaran masyarakat dalam pengelolaan sampah. Kegiatan berikutnya adalah sosialisasi kepada anak-anak, pemuda, dan orang tua di lingkungan RW 05 Desa Cisempur. Masyarakat RW 05 Desa Cisempur tertarik akan pengelolaan sampah yang berbasis daur ulang dan pemanfaatan sampah.

Kelimpahan Bakteri Coliform Pada Air Laut, Sedimen dan Foraminifera Jenis Calcarina Di Ekosistem Terumbu Karang Pulau Pramuka, Kepulauan Seribu, DKI Jakarta Yuliadi, Lintang Permatasari; Askar, Agus Tri; Agung, Mochamad Untung Kurnia; Andriani, Yuli
Jurnal Akuatika Indonesia Vol 3, No 1 (2018): Jurnal Akuatika Indonesia (JAkI)
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Padjadjaran

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Bakteri coliformÂ sudah lama dikenal sebagai bioindikator cemaran bahan organik di suatu perairan. Perairan laut merupakan muara bagi semua jenis limbah baik organik maupun anorganik yang berasal dari daratan. Foraminifera yang merupakan diatom bentik yang khas ditemukan di ekosistem terumbu karang juga dapat terkontaminasi bakteri coliformÂ pada perairan yang mengalami kondisi pencemaran bahan organik. Penelitian ini bertujuan untuk mengetahui nilai kelimpahan bakteri coliformÂ padaÂ air laut, sedimen danÂ foraminifera jenisÂ calcarina. Penelitian ini dilaksanakan dari bulan Maret sampai Agustus 2017 di Laboratorium Mikrobiologi dan BioteknologiÂ Molekuler (MICROMOL)Â Fakultas Perikanan dan Ilmu Kelautan (FPIK) Universitas Padjadjaran. Metode yang digunakan pada penelitian ini adalah metode Most Probable Number (MPN) yang dibandingkan denganÂ standar baku mutuÂ Kep-51/MENKLH/2004. Hasil penelitian menunjukkan bahwa kelimpahan bakteri coliform pada air laut yaitu 9->1100 MPN/100Â mL, pada sedimen sebesar 7-803,33 MPN/100Â gÂ dan yang ditemukan pada foraminiferaÂ jenisÂ calcarinaÂ berkisar <3-16 MPN/100Â g. Nilai bakteri coliform yang didapat pada sampel sedimen dan foraminifera masih dibawah ambang batas baku mutu Kementrian Lingkugan Hidup Nomor 51 tahun 2004. Namun, Pada sampel air laut hanya stasiun 1 dan stasiun 2 sudah melebihi batas ambang baku mutu yaitu 1100 MPN/100Â ml.

Transposon Insertion Phenomenon during Cloning of a Partial Fragment Derived from Metagenomic DNA Isolated from Deep-Sea Water and Sediment of Kawio Island, North Sulawesi Agung, Mochamad Untung Kurnia; Moeis, Maelita Ramdani
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 3 (2013): December 2013
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v8i3.84

Transposon is well-known as mobile element found abundant both in prokaryote and eukaryote genomes. In bacteria, transposon (famous name of a transposable DNA) could jump from chromosome to plasmid and its contrary. One type of transposons in bacteria known as insertion sequence (IS), it does not contain any additional genes except a gene encoding transposase, an enzyme that correlated to transponsition activities. The finding of transposon insertion unfortunately found during cloning of a fragment derived from deep-sea metagenomic DNA in this research. In the initial, this research was aimed to clone and characterize the Ã¡-amylase encoded gene derived from metagenomic DNA isolated from deep-sea water and sediment of Kawio Island, North Sulawesi. Metagenomic DNA has been isolated from deep-sea water and sediment and by using Whole Genome Amplification (WGA) technique, the DNA it could be increased in quantities to 146,31 ng for each 1 ng of metagenomic DNA. A fragment of ~1000 bp in length was obtained by using touchdown PCR method. The presence of a transposon in this DNA fragment is proposed as a hypothesis for losing ~700 bp leaving just 310 bp cloned sequence. Analysis of sequencing result showed a highest similarity between this 310 bp partial fragment with a replication protein (Rep) encoded gene from Pseudomonas putida (Query Coverage: 88%; Max. Identity: 80%, Positive: 86%) and this protein is known to be involved in plasmid replication where transposase encoding genes known usually presence together with this gene (Rep gene) in a bacterial plasmid.

Kelimpahan Bakteri Coliform Pada Air Laut, Sedimen dan Foraminifera Jenis Calcarina Di Ekosistem Terumbu Karang Pulau Pramuka, Kepulauan Seribu, DKI Jakarta Askar, Agus Tri; Agung, Mochamad Untung Kurnia; Andriani, Yuli; Yuliadi, Lintang Permatasari
Jurnal Akuatika Indonesia Vol 3, No 1 (2018): Jurnal Akuatika Indonesia (JAkI)
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan Universitas Padjadjaran, Grha. Kandaga (P

Bakteri coliform sudah lama dikenal sebagai bioindikator cemaran bahan organik di suatu perairan. Perairan laut merupakan muara bagi semua jenis limbah baik organik maupun anorganik yang berasal dari daratan. Foraminifera yang merupakan diatom bentik yang khas ditemukan di ekosistem terumbu karang juga dapat terkontaminasi bakteri coliform pada perairan yang mengalami kondisi pencemaran bahan organik. Penelitian ini bertujuan untuk mengetahui nilai kelimpahan bakteri coliform pada air laut, sedimen dan foraminifera jenis calcarina. Penelitian ini dilaksanakan dari bulan Maret sampai Agustus 2017 di Laboratorium Mikrobiologi dan Bioteknologi Molekuler (MICROMOL) Fakultas Perikanan dan Ilmu Kelautan (FPIK) Universitas Padjadjaran. Metode yang digunakan pada penelitian ini adalah metode Most Probable Number (MPN) yang dibandingkan dengan standar baku mutu Kep-51/MENKLH/2004. Hasil penelitian menunjukkan bahwa kelimpahan bakteri coliform pada air laut yaitu 9->1100 MPN/100 mL, pada sedimen sebesar 7-803,33 MPN/100 g dan yang ditemukan pada foraminifera jenis calcarina berkisar <3-16 MPN/100 g. Nilai bakteri coliform yang didapat pada sampel sedimen dan foraminifera masih dibawah ambang batas baku mutu Kementrian Lingkugan Hidup Nomor 51 tahun 2004. Namun, Pada sampel air laut hanya stasiun 1 dan stasiun 2 sudah melebihi batas ambang baku mutu yaitu 1100 MPN/100 ml.

BIOPROSPEK AKTIVITAS METABOLIT SEKUNDER DARI LAMUN (Seagrass) DAN BEBERAPA JENIS MAKROALGA (Seaweeds) SEBAGAI KANDIDAT AGEN ANTIBAKTERI PATOGEN Vibrio harveyi YANG MENYERANG UDANG WINDU (Penaeus monodon) (Studi in-vitro) Kurnia Agung, Mochamad Untung; Mulyani, Yuniar; Riyantini, Indah
Jurnal Harpodon Borneo Vol 5, No 1 (2012): Volume 5 No 1 April 2012
Publisher : Jurnal Harpodon Borneo

Bacterial deseases are seriousness problem in aquaculture. The uncontrolled of quality degradation of pond sites because of wastes and organic matters decompotition and also global climate changes has been predicted affect triggered the bacterial infections. Vibrio harveyi is one of patogenic bacteria which againts Tiger shrimp (Penaeus monodon). The recovery strategies has been applied both in the rehabilitation of pond sanitary and also it has been focussed in curative methods to overcome infection activities. The usage of synthetic antibiotics has been reported gift any negative effects, either for environment and rised pathogenic resintance because of uncontrolled exposure.Environmental friendly and based on biological methods has been promoted to overcome this problem. The usage of antibacterial agents derived from natural compounds has been recommended as an effective method. Several marine resources has been predicted as candidates of antibacterial agents againts Vibrio harveyi. Several seaweeds (macroalga) and seagrass has been explored in this research at in-vitro level of study to inhibit the activity of Vibrio harveyi. The result showed that all of the crude extracts derived from seagrass (Thallasia sp) and several kinds of seaweeds (Padina sp, Gracilaria sp and Sargassum sp) could inhibit the growth of Vibrio harveyi from the lowest concentration level (10 µg/mL). The highest inhibition activity has been showed from the antibacterial sensitivity test of crude extract of Thallasia sp at the concentration level of 10.000 µg/mL. Keywords : macroalgae, seagrass, secondary metabolite, antibacteria, Vibrio harveyi

BIOPROSPEK AKTIVITAS METABOLIT SEKUNDER DARI LAMUN (Seagrass) DAN BEBERAPA JENIS MAKROALGA (Seaweeds) SEBAGAI KANDIDAT AGEN ANTIBAKTERI PATOGEN Vibrio harveyi YANG MENYERANG UDANG WINDU (Penaeus monodon) (Studi in-vitro) Mochamad Untung Kurnia Agung; Yuniar Mulyani; Indah Riyantini
Jurnal Harpodon Borneo Vol 5, No 1 (2012): Volume 5 No 1 April 2012
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v5i1.78

Bacterial deseases are seriousness problem in aquaculture. The uncontrolled of quality degradation of pond sites because of wastes and organic matters decompotition and also global climate changes has been predicted affect triggered the bacterial infections. Vibrio harveyi is one of patogenic bacteria which againts Tiger shrimp (Penaeus monodon). The recovery strategies has been applied both in the rehabilitation of pond sanitary and also it has been focussed in curative methods to overcome infection activities. The usage of synthetic antibiotics has been reported gift any negative effects, either for environment and rised pathogenic resintance because of uncontrolled exposure.Environmental friendly and based on biological methods has been promoted to overcome this problem. The usage of antibacterial agents derived from natural compounds has been recommended as an effective method. Several marine resources has been predicted as candidates of antibacterial agents againts Vibrio harveyi. Several seaweeds (macroalga) and seagrass has been explored in this research at in-vitro level of study to inhibit the activity of Vibrio harveyi. The result showed that all of the crude extracts derived from seagrass (Thallasia sp) and several kinds of seaweeds (Padina sp, Gracilaria sp and Sargassum sp) could inhibit the growth of Vibrio harveyi from the lowest concentration level (10 µg/mL). The highest inhibition activity has been showed from the antibacterial sensitivity test of crude extract of Thallasia sp at the concentration level of 10.000 µg/mL. Keywords : macroalgae, seagrass, secondary metabolite, antibacteria, Vibrio harveyi

Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance Yuniar Mulyani; Karimah Syakirotin; Kiki Haetami; Yeni Mulyani; Evi Liviawaty; Mochamad Untung Kurnia Agung
Jurnal Biodjati Vol 5, No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5263

The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chloramphenicol, ampicillin, and metronidazole were used to determine the Bacillus resistance to antibiotics using the Kirby Bauer method. The resistance assay was carried out with a bacterial density of 108 CFU/mL. The results showed that Bacillus isolates derived from common carp intestine were closely related and resistant to antibiotics. Isolates that resistant to the three types of antibiotics were Cc.1.9 (CgN9) and Cc.2.18 (CgM18).

Antibacterial Activity of Haslea ostrearia Supernatant Adapted in Indonesia against Pathogenic Bacteria Relevant to Mariculture (In-Vitro Study) Ikfa Permatasari; Fiddy Semba Prasetiya; Sulastri Arsad; Mochamad Untung Kurnia Agung; Jean-Luc Mouget
Journal Omni-Akuatika Vol 15, No 1 (2019): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Haslea ostrearia has known as the only diatom which synthesizes a water-soluble blue pigment, marennine. It has some biological activities such as allelopathy, antioxidant, antibacterial, antiviral, and growth inhibitor. Marennine is available in two forms, intracellular which located in the apical of the cell and extracellular which released into the culture medium. This research aimed to test the bioactivity of Haslea ostrearia supernatant adapted in Indonesia as an antibacterial against pathogenic bacteria relevant to marine culture using in-vitro study. This research was using an explorative method, data analysed statistically and descriptive-comparatively observed. Observation parameters were the inhibitory zone of antibacterial activity, absorbance value of MIC test, and bacterial growth qualitatively from MBC test. Marennine concentration in the supernatant of Haslea ostrearia adapted in Indonesia is about 3.74 mg. L-1. The highest concentration supernatant of Haslea ostrearia that shown antibacterial activity is 3.5 mg. L-1 with inhibitory zone diameter is about 6.87 mm for Staphylococcus aureus and 7.14 mm for Vibrio harveyi, correspondingly. The minimum concentration that inhibits the growth of Staphylococcus aureus is 0.03 mg. L-1, while for Vibrio harveyi is 0.06 mg. L-1. Antibacterial activity of supernatant Haslea ostrearia originally adapted in Indonesia classified into bacteriostatic

Study of Symbiont Bacteria of Acropora digitifera Coral From Ciletuh Bay, Sukabumi by Using Culture and Molecular Approach Nurfitri Nurfitri; Fiddy Semba Prasetiya; Indah Riyantini; Yuniar Mulyani; Sulastri Arsad; Mochamad Untung Kurnia Agung
Journal Omni-Akuatika Vol 15, No 2 (2019): Omni-Akuatika November
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2019.15.2.748

Bacteria are one of the prokaryotic microorganisms that are symbiotic with coral reefs. These microorganisms help corals in secreting mucus layers which are used as a place to live for bacteria and control the presence of pathogenic bacteria in corals. The purpose of this study was to determine the diversity of bacterial communities associated with Acropora digitifera corals in Ciletuh bay, West Java, by culture approach. Sampling was carried out at a depth of 4 meters. Morphology-based identification and molecular approach based on sequence of the small ribosomal unit (16S) rRNA gene were used to determine the bacterial species in the samples. A total of six pure isolates were identified based on morphological observation and the molecular DNA characteristics from three of them were identified with 16S rRNA gene sequences. The identification using 16S rRNA gene showed that the isolate ACD.P4.PH7.P had a close relationship with the BF strain and zb strain of Bacillus flexus (acc number MH569560.1) with a similarity of 85.44 %. While ACD.P4.PH9.P isolate has a close relationship with Bacillus sp. c234 (acc number FJ950647.1) with a similarity of 98.50 %. Additionally, ACD.P4.PH9.K isolates closely related to Bacillus sp. of strain 6RM1 (acc number MK134607.1) with a similarity of 94.78 %. This study also revealed that both microscopic identifications by morphological traits and molecular approach using 16S rRNA gene can be used in bacterial diversity assessment.

Analisis Kandungan Logam Berat Timbal (Pb) Pada Makrozoobenthos Di Kawasan Mangrove Desa Pusakajaya Utara Kecamatan Cilebar Karawang Ahmad Tidjani; Walim Lili; Mochamad Untung Kurnia Agung
Jurnal Perikanan Kelautan Vol 7, No 2 (2016): Jurnal Perikanan dan Kelautan
Publisher : Fakultas Perikanan dan Ilmu Kelautan Universitas Padjadjaran

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Tujuan penelitian ini adalah untuk mengetahui konsentrasi logam timbal pada makrozoobenthos, di kawasan mangrove Desa Pusakajaya Utara Kecamatan Cilebar Kabupaten Karawang. Metode yang digunakan dalam penelitian ini adalah metode survei. Pengukuran kandungan logam berat pada air, sedimen dan makrozoobenthos dengan menggunakan metode potensiometrik. Makrozoobenthos di kawasan mangrove Desa Pusakajaya Utara yang teridentifikasi terdiri dari 5 genus yang seluruhnya tergolong dalam filum moluska dan kedalam kelas gastropoda. Kecuali 1 genus yang tergolong dalam filum arthropoda dan kedalam kelas malacostraca. Kelimpahan makrozoobenthos paling rendah berada di Stasiun 2 dengan kepadatan 171 individu/m2 dan kelimpahan tertinggi berada pada Stasiun 1 dengan kepadatan 236 individu/m2. Kandungan logam timbal pada makrozoobenthos berkisar antara 22,431 – 25,222 mg/kg secara umum lebih rendah dari kandungan logam timbal pada sedimen dengan kandungan logam timbal berkisar antara 31,897 – 36,689 mg/kg sementara kandungan logam timbal pada air berkisar antara 5,398 – 6,670 ppm.

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