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All Journal Jurnal Perikanan Kelautan Jurnal Harpodon Borneo Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Akuatika Indonesia Jurnal Biodjati Omni-Akuatika Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan Jurnal Kreativitas PKM Hydrogen: Jurnal Kependidikan Kimia Farmers: Journal of Community Services
Mochamad Untung Kurnia Agung
Fakultas Perikanan Dan Ilmu Kelautan, Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Public Health Social Sciences Other

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ANALYSIS OF BACTERIAL ABUNDANCE IN THE INTESTINES OF COMMON CARP Cyprinus carpio TREATED WITH THE PROBIOTIC Bacillus subtilis Alin Shelina Nurashila; Yuniar Mulyani; Zuzy Anna; Mochamad Untung Kurnia Agung
AQUASAINS Vol 11, No 2 (2023)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/aqs.v11i2.p1269 - 1276

Abstract

Efforts to increase microbial populations in fish intestines tract can be done by utilizing microbes in the fish's digestive tract as probiotics. The application of Bacillus subtilis in fish feed mixtures can provide better growth performance in modifying microbes in the intestines of fish. The aim of this research was to analyze the bacterial community in the intestines of Common Carp(Cyprinus carpio) that have been given probiotics from the bacteria Bacillus subtilis with metagenome sequencing using 16S rRNA through Next Generation Sequencing (NGS) technology. Common carp samples were collected from maintenance conducted with different treatments, namely control fish without probiotic administration and fish treatment with mixed feed probiotic Bacillus subtilis. This research was conducted in the Laboratory of Fisheries Biotechnology, Faculty of Fisheries and Marine Science, Padjajaran University. Then molecular identification was carried out using Illumina's NGS in Novogene, Singapore. The results of the identification of using probiotic bacteria Bacillus subtilis in Common carp(Cyprinus carpio) feed affect the bacterial community in the fish intestine. In the intestines of carp that were given probiotics, the highest abundance was Proteobacteria, Fusobacteriota, and firmicutes.The highest abundance at the genus level in both samples was domiciled by the genus Cetobacterium followed by the Sphingomonas and Aeromonas.
Pengenalan Marine Citizenship melalui Pendidikan Lingkungan bagi Siswa SDN 02 Bagolo, Kalipucang Pangandaran Santi Rukminita Anggraeni; Mochamad Untung Kurnia Agung
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 6, No 10 (2023): Volume 6 No 10 2023
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v6i10.11648

Abstract

ABSTRAK Bagolo merupakan salah satu daerah tujuan wisata pantai di wilayah Pangandaran. Pesisir Bagolo adalah merupakan habitat alami rumput laut dan lamun. Marine citizenship merupakan pelibatan berbagai lapisan masyarakat untuk berperan aktif melestarikan laut dan sumberdayanya. Generasi muda memainkan peran strategis pengelolaan laut secara lestari dalam jangka panjang. Kegiatan pengabdian kepada masyarakat (PKM) ini bertujuan mengenalkan marine citizenship kepada siswa sekolah dasar di Desa Bagolo.Tahapan kegiatan pengabdian masyarakat terdiri dari persiapan, pelaksanaan dan evaluasi. Hasil wawancara dan observasi pada tahap persiapan kemudian diformulasi menjadi bentuk pendidikan lingkungan bagi 49 orang siswa kelas 4 dan 5 di SDN 02 Bagolo. Aktivitas pendidikan lingkungan yang dilakukan terdiri dari pemberian materi dan evaluasi pengetahuan siswa tentang jenis dan nilai penting sumberdaya hayati yang ada di pantai Karapyak, membuat herbarium dan pengawetan spesimen basah. Indikator tercapainya tujuan pengabdian kepada masyarakat adalah keterlibatan aktif siswa dalam proses diskusi untuk evaluasi pemahaman terhadap materi dan proses pembuatan awetan biota laut. Program pendidikan lingkungan yang lebih sistematis dan berkala yang juga melibatkan pihak guru dan orang tua diperlukan agar pengetahuan siswa dapat menjadi bagian dari pembentukan karakter peduli lingkungan dan laut. Kata Kunci: Herbarium, Karakter Siswa, Partipasi Pengelolaan Laut  ABSTRACT Bagolo is one of the beach tourism destinations in the Pangandaran region. Bagolo Coast is a natural habitat for seaweed and sea grass. Marine citizenship is the involvement of various groups of society to actively support the sustanability of the sea and its resources. The young generation plays a strategic role in long-term sustainable marine management. This community service activity (PKM) aimed to introduce maritime citizenship to elementary school students in Bagolo Village. The stages of community service activities consist of preparation, implementation and evaluation. The results of the interviews and observations during the preparatory stage were then formulated into a form of environmental education for 49 students in grades 4 and 5 at SDN 02 Bagolo. Environmental education activities consist of providing material and evaluating students' knowledge about type and the importance of biological resources on the Karapyak beach, making herbarium and preserving wet specimen. An indicator of goal achievement was the active involvement of students in the discussion process to evaluate understanding of the material and the process of making marine biota preservation. Further systematic and periodic environmental education program involving teachers and parents is needed so that students' knowledge can become part of the character development of caring the environment and the sea. Keywords: Herbarium, Student Character, Marine Management Participation
Penyuluhan Daring Manajemen Kualitas Air untuk Budidaya Ikan dalam Ember di Desa Cipacing, Kecamatan Jatinangor, Kabupaten Sumedang, Jawa Barat Yuniar Mulyani; Dian Yuni Pratiwi; Mochamad Untung Kurnia Agung
Farmers: Journal of Community Services Vol 2, No 1 (2021)
Publisher : Unpad Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/fjcs.v2i1.31546

Abstract

Budidaya ikan dalam ember (budikdamber) dapat menjadi solusi untuk meningkatkan ketahanan pangan dan alternatif usaha di tempat yang lahanya terbatas seperti Desa Cipacing. Budidaya ikan dalam ember juga dapat menjadi salah satu alternatif usaha untuk meningkatkan perekonomian di masyarakat. Salah satu faktor yang paling penting dalam melakukan budidaya ikan dalam ember adalah kualitas air. Kualitas air yang buruk dapat menyebabkan kematian pada ikan sehingga menurunkan hasil panen budidaya. Penyuluhan daring ini bertujuan untuk memberikan pengetahuan tentang manajemen kualitas air pada budidaya ikan dalam ember. Informasi ini penting untuk diketahui agar masyarakat mampu melakukan budidaya ikan dalam ember dengan manajemen kualitas air yang baik. Penyuluhan ini dilakukan melalui dua tahapan yaitu persiapan dan pelaksanaan. Pelaksanaan dilakukan secara daring diikuti oleh anggota karang taruna Desa Cipacing sebanyak 15 orang. Sebagian besar peserta belum memahami mengenai kualitas air pada budidaya ikan dalam ember. Setelah penyuluhan, masyarakat telah mampu memahami manajemen kualitas air untuk budidaya ikan dalam ember.Kata Kunci: Ikan, Budikdamber, Kualitas air, Penyuluhan daring, Cipacing
THE 16S rRNA GENE MARKER-BASED MOLECULAR IDENTIFICATION OF CULTURABLE COLIFORM BACTERIA ISOLATED FROM FORAMINIFERA CALCARINA DERIVED FROM PRAMUKA ISLAND WATERS, THE SERIBU ISLAND DISTRICT, JAKARTA PROVINCE Agung, Mochamad Untung Kurnia -; Askar, Agus Tri; Andriani, Yuli; Yuliadi, Lintang Permatasari
Jurnal Harpodon Borneo VOLUME 13 NO. 1 APRIL 2020
Publisher : Fakultas Perikanan Dan Ilmu Kelautan Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/harpodon.v13i1.1404

Abstract

Contamination of coliform bacteria in benthic foraminifera has been reported due to pollution of organic wastes in the aquatic environment around coral reef ecosystems and this event was known to interfere the process of foraminifera shell formation which in turn resulted the disruption of the role of foraminifera in the process of formation of coral reef bottom sediments. The aim of this research is to identify the isolates of culturable coliform bacteria that contaminate foraminifera Calcarina species isolated from the waters of the Pramuka Island, the Seribu Island district, Jakarta Province using the 16S rRNA gene markers. Foraminifera sampling was carried out in the waters of Pramuka Island, the Seribu Island district, Jakarta Province in 5 (five) stations, while the process of bacterial isolation and molecular identification were carried out at the Laboratory of Microbiology and Molecular Biotechnology (MICROMOL), Faculty of Fisheries and Marine Sciences (FPIK), University Padjadjaran. Molecular identification was carried out using the Polymerase Chain Reaction (PCR) method based on the 16S rRNA gene markers. Sequencing is done by sending PCR results to 1st Base, sequencing service company, in Singapore and then, the aligning of sequencing results with databases in genBank was done using  the Basic Local Alignment Search Tool (BLASTTM) program available on the National Center for Biotechnology Information (NCBI) website. The results of 16S rRNA gene amplification from the five isolates produced amplicons of ± 1400 bp length with concentrations ranging from 157.5 µg / mL-230 µg / mL and with a purity ratio ranging from 1.477-1.769. While the results of BLAST and phylogenetic analysis showed that the five isolates were closely related to the isolate Eschericia coli strain inspire99 (Acc No. JQ315935.1), which was isolated from the waters of the Bay of Bengal, India. These results also indicate the existence of ecological connectivity between the waters of the Bay of Bengal in India and the waters of Pramuka Island in Indonesia.
Antioxidant of Ethanol Extract and Toxicity of Fractions from Aspergillus unguis a Marine Sponge Symbiont of Aaptos suberitoides Masyerli, Scify Bilqis Nawafi; Efdi, Mai; Bashari, Muhammad Hasan; Untung Kurnia Agung, Mochamad; Subhan, Beginer; Efahmi, Efahmi; Andriani, Yosie; Syafrizayanti, Syafrizayanti
Hydrogen: Jurnal Kependidikan Kimia Vol 13, No 1 (2025): February 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i1.14645

Abstract

The marine sponge Aaptos suberitoides has been shown to have anticancer properties, with clear evidence of its capacity to suppress the growth of cancer cells. However, the pharmaceutical exploration of chemicals from marine organisms causes significant environmental concerns. Aspergillus unguis, a fungal symbiont of the marine sponge A. suberitoides, has been isolated for its potential in sustainable natural products resources. This study assesses the antioxidant activity of ethanol extract and the toxicity of four levels fractions from ethanol extracts of A. unguis mycelium and these results were reported for the first time in this study. The antioxidant of ethanol extract was determined using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The fractions were examined for toxicity using the Brine Shrimp Lethality Test (BSLT). The ethanol extract of A. unguis shows very strong antioxidant bioactivity (IC50 = 42.84 mg/L). The LC50 values for hexane, chloroform, ethyl acetate, and butanol fractions were determined to be 74.11 µg/mL, 93.84 µg/mL, 59.37 µg/mL, and 142.79 µg/mL, respectively. It indicates significant toxicity. These preliminary results are important knowledge for further research into the bioactivity potential of the metabolites as candidate anticancer compounds, aligning with marine pharmaceutical drug development.
Antioxidant of Ethanol Extract and Toxicity of Fractions from Aspergillus unguis a Marine Sponge Symbiont of Aaptos suberitoides Masyerli, Scify Bilqis Nawafi; Efdi, Mai; Bashari, Muhammad Hasan; Untung Kurnia Agung, Mochamad; Subhan, Beginer; Efahmi, Efahmi; Andriani, Yosie; Syafrizayanti, Syafrizayanti
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 1 (2025): February 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i1.14645

Abstract

The marine sponge Aaptos suberitoides has been shown to have anticancer properties, with clear evidence of its capacity to suppress the growth of cancer cells. However, the pharmaceutical exploration of chemicals from marine organisms causes significant environmental concerns. Aspergillus unguis, a fungal symbiont of the marine sponge A. suberitoides, has been isolated for its potential in sustainable natural products resources. This study assesses the antioxidant activity of ethanol extract and the toxicity of four levels fractions from ethanol extracts of A. unguis mycelium and these results were reported for the first time in this study. The antioxidant of ethanol extract was determined using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. The fractions were examined for toxicity using the Brine Shrimp Lethality Test (BSLT). The ethanol extract of A. unguis shows very strong antioxidant bioactivity (IC50 = 42.84 mg/L). The LC50 values for hexane, chloroform, ethyl acetate, and butanol fractions were determined to be 74.11 µg/mL, 93.84 µg/mL, 59.37 µg/mL, and 142.79 µg/mL, respectively. It indicates significant toxicity. These preliminary results are important knowledge for further research into the bioactivity potential of the metabolites as candidate anticancer compounds, aligning with marine pharmaceutical drug development.
Molecular Identification of Bacillus Derived from the Common Carp Intestine and Their Antibiotics Resistance Mulyani, Yuniar; Syakirotin, Karimah; Haetami, Kiki; Mulyani, Yeni; Liviawaty, Evi; Agung, Mochamad Untung Kurnia
Jurnal Biodjati Vol 5 No 2 (2020): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v5i2.5263

Abstract

The use of bacteria as a biological control agent in aquaculture is such new hope overcoming the negative impacts of the impropriate use of antibiotics. Bacillus is widely known as a potential probiotic for aquaculture, especially which are derived from aquatic biota. Due to a concern that the role of Bacillus as a probiotic agent will be disrupted by residues of antibiotics in the water, this research aimed to identify Bacillus species from common carp intestine based on the molecular method and investigate their resistance to some com-mercial antibiotics that widely used in aquaculture. Chloramphenicol, ampicillin, and metronidazole were used to determine the Bacillus resistance to antibiotics using the Kirby Bauer method. The resistance assay was carried out with a bacterial density of 108 CFU/mL. The results showed that Bacillus isolates derived from common carp  intestine were closely related and resistant to antibiotics. Isolates that resistant to the three types of antibiotics were Cc.1.9 (CgN9) and Cc.2.18 (CgM18).
Co-Authors Agid Faisal Harahap Agus Tri Askar Ahmad Tidjani Aini Iftinaan K J Alin Shelina Nurashila Askar, Agus Tri Askar, Agus Tri Bashari, Muhammad Hasan Beginer Subhan Dede Supriatna Dian Yuni Pratiwi Dinur Piranto Donny Juliandri Prihadi Efahmi, Efahmi Evi Liviawaty Fiddy Semba Prasetiya Fiddy Semba Prasetiya Ibnu Dwi Buwono Iis Rostini Ikfa Permatasari Indah Riyantini Indah Riyantini Indah Riyantini Indah Riyantini Iskandar - Jean-Luc Mouget Karimah Syakirotin Kiki Haetami Lintang Permatasari Yuliadi MAELITA RAMDANI MOEIS Mai Efdi Masyerli, Scify Bilqis Nawafi Mega Laksmini Syamsudin Mochamad Rudyansyah Ismail Muhamad Ali Rahman Muhamad Hariza Kurniawan Nurfitri Nurfitri Rita Rostika Santi Rukminita Anggraeni Sri Astuty Sri Astuty Sriati - Suci - Febnikayani Sulastri Arsad Sulastri Arsad sunarto sunarto Syafrizayanti, Syafrizayanti Syakirotin, Karimah Tengku Alwie Petra Sya’bani Titin Herawati Wahyuniar Pamungkas Walim Lili Yeni Mulyani Yosie Andriani Yuli Andriani Yuli Andriani Yuliadi, Lintang Permatasari Yuliadi, Lintang Permatasari Yuliadi, Lintang Permatasari Yuniar Mulyani Zuzy Anna