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All Journal Agrikultura AGROTEK: Jurnal Ilmiah Ilmu Pertanian
Subakti Putri, Syifa Nabilah
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Pengaruh Beberapa Pelarut Ekstrak Daun Nerium oleander L. terhadap Mortalitas, Konsumsi Makan dan Kelulushidupan Spodoptera frugiperda J.E. Smith Subakti Putri, Syifa Nabilah; Susanto, Agus; Bari, Ichsan Nurul; Santriyani, Ai Siti
Agrikultura Vol 33, No 3 (2022): Desember, 2022
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v33i3.41671

Abstract

Tanaman beracun oleander (Nerium oleander) memiliki potensi untuk mengendalikan hama utama tanaman jagung, salah satunya ulat grayak jagung (Spodopetera frugiperda). Namun demikian, studi ekstrak daun N. oleander yang disiapkan dengan menggunakan pelarut yang berbeda belum diketahui efektivitasnya dalam mengendalikan S. frugiperda. Penelitian ini bertujuan untuk mengetahui perbedaan berbagai pelarut ekstrak daun N. oleander terhadap terhadap mortalitas, konsumsi makan, dan kelulushidupan larva S. frugiperda. Penelitian melibatkan ekstraksi daun N. oleander berdasarkan perbedaan polaritas pelarut (n-hexane, etanol, metanol, air) untuk lima konsentrasi uji yang berbeda serta perlakuan kontrol yang diulang empat kali menggunakan Rancangan Acak Lengkap (RAL) pada larva S. frugiperda instar III. Hasil penelitian menunjukkan bahwa ekstrak kasar metanol daun N. oleander memberikan pengaruh penghambatan konsumsi pakan larva S. frugiperda sebesar 0,071 ± 0,028 g dibandingkan kontrol 0,642 ± 0,031 g. Selain itu, ekstrak kasar metanol daun N. oleander menunjukkan persentase mortalitas tertinggi sebesar 97,5 ± 0,50%, diikuti oleh ekstrak kasar air 70 ± 0,82%, ekstrak kasar etanol 57,50 ± 0,96%, dan ekstrak kasar n-hexane 50,00 ± 0,82%. Kelulushidupan larva, pupa, dan imago S. frugiperda menunjukkan nilai persentase terendah sebesar 2-3,4% ketika diaplikasikan ekstrak kasar metanol daun N. oleander. Secara keseluruhan, metanol adalah pelarut yang paling baik untuk mengekstrak daun N. oleander terhadap mortalitas, daya konsumsi, dan kelulushidupan larva S. frugiperda.
THE EFFECT OF METHANOL EXTRACT OF Cerbera odollam LEAVES ON LOCOMOTION AND EXPLORATION ACTIVITIES OF Spodoptera frugiperda LARVAE Subakti Putri, Syifa Nabilah; Susanto, Agus; Bari, Ichsan Nurul
AGROTEK: Jurnal Ilmiah Ilmu Pertanian Vol 9, No 2 (2025): September
Publisher : Percetakan Umi Toaha Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/agrotek.v9i2.958

Abstract

The fall armyworm (FAW), Spodoptera frugiperda, is one of the major pests of maize, exhibiting high adaptability and causing significant losses to agriculture in Indonesia. An environmentally friendly approach to controlling FAW infestations involves using local toxic plants, such as Cerbera odollam, also known as Bintaro, which contains cardenolide glycosides, saponins, and alkaloids with potential as botanical insecticides. This study aimed to evaluate the effect of the methanol extract of C. odollam leaves on the locomotion and exploration activities of FAW larvae. The test was conducted using the leaf dip method at concentrations of 0, 1000, 2000, and 4000 ppm, with six replications. The samples were then analyzed and observed using the Organism Video Tracking Application ToxTrac v2.96. Results showed that higher extract concentrations led to a decrease in larval speed, from 17.48 mm/s to 1.83 mm/s, and a reduction in exploration area, from 48 to 9. In addition, larval immobility duration (freeze activity) increased nearly sevenfold compared to the control. These findings indicate that C. odollam shows promise as a natural insecticide that repels FAW larvae by limiting their movement and exploratory behavior.
Co-Authors Agus Susanto Bari, Ichsan Nurul Santriyani, Ai Siti Wiwik Handayani