Lindung Tri Puspasari
Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Padjadjaran

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Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Paramita, Hedi; Puspasari, Lindung Tri; Maharani, Rani; Supratman, Unang; Hidayat, Yusup; Meliansyah, Rika; Dono, Danar
CROPSAVER - Journal of Plant Protection Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

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Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Potensi Cuka Kayu Pinus dalam Pengendalian Penyakit Antraknosa pada Cabai Merah Sri Hartati; Rika Meliansyah; Lindung Tri Puspasari
Jurnal Fitopatologi Indonesia Vol 9 No 6 (2013)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (332.374 KB) | DOI: 10.14692/jfi.9.6.173

Abstract

Anthracnose disease caused by Colletotrichum capsici is a major disease on red chili which causes great economic losses. Wood vinegar, as a result of condensation of smoke, can be used as bactericide and fungicide. The use of pine wood vinegar is prospective for controlling anthracnose disease. The objective of this research was to study the potency of pine wood vinegar in controlling anthracnose on red chili. In vitro test was carried out to measure the inhibition of pine wood vinegar against the growth of mycelium mass and the germination of the conidium of C. capsici. The treatments were the concentration of the pine wood vinegar, i.e. 0, 1.5, 2.5, 3.5, 4.5, and 5 %, and a fungicide (2% of antracol) as comparison. The results showed that the lower the concentration of the pine wood vinegar, the lower the inhibition of the mycelium growth. The highest inhibition of the mycelium growth (76.1%) was caused by 5% of the pine wood vinegar, followed by 41.04% inhibition caused by 4.5% concentration. There was no germination of the conidium on all treatments.
Effect of Temperature, Storage Time, The Residual Test of Neem Oil Formulation (Azadirachta indica A. JUSS) and Bitung Formulation (Baringtonia asiatica) to Its Toxicity Against Large Cabbage Heart Caterpillar (Crocidolomia pavonana F) Neneng Sri Widayani; Afifah Nashirotul Haq; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.433 KB) | DOI: 10.24198/cs.v1i1.16996

Abstract

Effectiveness of pesticide affected by many factors such as physical environment and duration of storage. This study aims to determine the effect of temperature and storage time of formulation of neem 50 EC and residual effect of formulation of neem 50 EC and formulation of bitung 30 SC against C. pavonana larvae. The formulations tested on the experiment of effect storage time and temperature test using the newly prepared, neem formulation has been stored for 19 months at room temperature 24.6-31.7oC, and stored in low temperature (4oC) as well as controls. The toxicity of the neem oil formulation was tested at concentrations of 1%, 2%, and 3%. The experiment of residual effect was performed by spraying of the formulation on broccoli leaf, then the leaf samples were taken on days 0,1,2,3,5,7,9,11, and 13 days after application of insecticide. The results of the research indicated that storage at low temperatures (temperature 4oC) for 19 months can maintain the toxicity of the neem formulation to C. pavonana larvae. while storage at room temperature for 19 months resulted in a decreased toxicity. The residual activity of the neem formulations at concentration of 0.8% and 2.4% were not caused mortality of C. pavonana larvae at the residual age of 13.34 days and 14.66 days. The activity of Bitung formulation at concentration of 1.1% and 2.8% were not caused mortality of C. pavonana larvae at the residual age of 11.33 days and 16.26 days.
Cases of Fall Army Worm Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae) Attack on Maize in Bandung, Garut and Sumedang District, West Java. Yani Maharani; Vira Kusuma Dewi; Lindung Tri Puspasari; Lilian Rizkie; Yusup Hidayat; Danar Dono
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.498 KB) | DOI: 10.24198/cropsaver.v2i1.23013

Abstract

Bioactivity Formulation Of Leaf Extract Of Kalanchoe pinnata And Seed Of Azadirachta indica Against Spodoptera litura Hedi Paramita; Lindung Tri Puspasari; Yusup Hidayat; Rika Meliansyah; Danar Dono; Rani Maharani; Unang Supratman
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.565 KB) | DOI: 10.24198/cs.v1i1.16995

Abstract

The aim of this research is to know the most effective concentration of liquid formulation of Kalanchoe pinnata leaf extract 50 EC and Azadirachta indica seed extract 50 EC against S. litura. The experiment was conducted at the Laboratory of Pesticides and Enviromental Toxicology, Department of Plant Pests and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The concentration were tested of each formulation were 1%, 2%, 3 %, 4%,and control.  The experiment arranged in completely randomized design (CRD) and replicated three times. The results showed that the formulation of A. indica seed extract 50 EC at concentration of 2%, 3%, and 4% effective against S. Litura larvae with mortality of 76.7%, 86.7, and 93.3%  This formulation also causing a decrease in feeding activity, weight, and lenghten the development time of S.litura larvae. Unlike the case with liquid formulations of K. pinnata 50 EC extract which was less toxic and did not show significant differences in feed intake, weight of larvae, and development time of larvae compared control treatment.                Keywords: Toxicity, mortality, growth dearragement, botanical insecticide.
Laboratory Evaluation of Neem formulation bioactivity against Crocidolomia pavonana F. larvae Raden Arif Malik Ramadhan; Neneng Sri Widayani; Lindung Tri Puspasari; Yusup Hidayat; Danar Dono
CROPSAVER Vol 1, No 1 (2018)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.31 KB) | DOI: 10.24198/cs.v1i1.20334

Abstract

This study aims to evaluate the bioactivity of the formulation of Neem 50 EC  against Crocidolomia pavonana larvae in the laboratory. The study using six treatments and three replications. The treatment consisted of the formulation of neem 50 EC at concentrations of 0.3%, 0.5%, 0.8%, 1.3%, 2.3%, and control. The treatment was done by leaf feeding method and tested on C. pavonana instar II. The results of the research showed that the formulation of neem 50 EC at concentration of 2.3% caused mortality of C. pavonana larvae with mortality rate of 95% and has LC50 value aqual to 0.83%. Neem formulation can inhibit the development of C. pavonana larvae from instar I to instar IV, decrease food consumption, and decrease the dry weight of C. pavonana larvae instar IV.Keywords: Azadirachta indica, Bioactivity, Crocidolomia pavonana, neem formulation
Kompatibilitas Vegetatif Fusarium oxysporum dari Beberapa Tanaman Inang Sri Sri Hartati; Ummu Salamah Rustiani; Lindung Tri Puspasari; Wawan Kurniawan
Agrikultura Vol 27, No 3 (2016): Desember, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (647.943 KB) | DOI: 10.24198/agrikultura.v27i3.10875

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ABSTRACTVegetatif compatibility of Fusarium oxysporum on various hostsMany strains or race of Fusarium oxysporum can be grouped based on compatibility reproduction from a variety of different strains called Vegetative Compatibility Group (VCG). This study was aimed to determine how the grouping of several isolates of F. oxysporum and grouping of several hosts of the fungus by vegetative compatibility group. Fusarium oxysporum isolated from chickpea plants that showed symptoms of fusarium wilt. The isolates of F. oxysporum of chili and tomatoes obtained from the culture collections of Mycology Laboratory of IPB. Stages of vegetative compatibility testing assayed through recovery of nit mutants, the identification of phenotype of nit mutant, and complementation test. There are 29 mutants isolated from the isolates of F. oxysporum. Nit1 mutant was obtained from all isolates of beans, tomatoes and peppers. NitM and Nit3 mutant isolates were obtained from chickpea 4 and chili sequentially. Two VCG and one single self compatibility (SSC) were assayed from isolates of F. oxysporum based on complementation testing.Keywords: Beans, Fusarium wilt, Nit mutant, SSC, VCGABSTRAKJamur Fusarium oxysporum memiliki banyak forma spesialis dan ras. Jamur ini dapat dikelompokkan berdasarkan kompatibilitas reproduksi dari berbagai strain yang berbeda disebut dengan vegetative compatibility group (VCG). Penelitian ini bertujuan untuk mengetahui cara pengelompokkan F. oxysporum dan pengelompokkan jamur tersebut dari beberapa inang berdasarkan kelompok kompatibilitas vegetatifnya. Isolasi F. oxysporum dilakukan dari tanaman kacang panjang yang menunjukkan gejala layu fusarium. Isolat F. oxysporum dari cabai dan tomat berasal dari koleksi Laboratorium Mikologi IPB. Tahapan pengujian kompatibilitas vegetatif melalui pembiakan nit mutan, identifikasi fenotipe nit mutan, dan pengujian komplementasi. Isolasi mutan F. oxysporum didapatkan 29 mutan. Mutan nit1 didapatkan dari semua isolat yang diperoleh dari semua inang yang berbeda yaitu kacang panjang, tomat dan cabai. Mutan nitM hanya didapatkan dari isolat kacang panjang 4 dan mutan nit3 hanya didapatkan dari isolat cabai. Berdasarkan uji komplementasi F. oxysporum yang diuji terdiri dari dua VCG dan satu single self compatibility (SSC).Kata Kunci: Kacang panjang, Layu fusarium, Nit mutant, SSC, VCG
Komposisi Komunitas Serangga Aphidophaga dan Coccidophaga pada Agroekosistem Kacang Panjang (Vigna sinensis l.) di Kabupaten Garut Lindung Tri Puspasari; Martua Suhunan Sianipar; Sri Hartati
Agrikultura Vol 27, No 1 (2016): April, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.077 KB) | DOI: 10.24198/agrikultura.v27i1.8474

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ABSTRACTComposition of Aphidophaga and Coccidophaga Insect Communities on Long Bean Agroecosystem (Vigna sismensis L.) at Garut RegencyThe research about Aphidophaga and Coccidophaga insect composition comunity on long beans (Vigna sinensis L.) agroecosystems was done in the month of April to November 2011 in Haruman Village, Leles District, Garut Regency. Research was conducted in the form of surveys which were done by collecting insects directly and using various traps ie yellow traps board, fitfall traps, and nets swinging. The dominant insect pest that found was Aphis craccivora which causing percentage of damage to the plants ranging from 20% to 90%. Types of aphidophaga and coccidophaga found were belong to the Order of Coleoptera : Family Coccinellidae Menochilus sexmaculatus, Micraspis sp., Harmonia sp., Verania lineata, Curinus coeruleus, Scymnus sp., Coccinella transversalis); the Order of Diptera : Family Syrphidae namely Ischiodon scutellaris; Neuroptera: Family Hemerobiidae; the Order of Diptera : Family Cecidomyiidae Aphidoletes aphidimyza; and there was also Carabidae beetles of the Order Coleoptera with species diversity index wasl relatively low. The highest abundance of predators of coccidophaga and aphidophaga group was dominated by Ischiodon scutellaris and Menochilus sexmaculatus.Key words: Diversity, Abundance, Dominant species, Insect pestsABSTRAKPenelitian mengenai komposisi komunitas serangga aphidophaga dan coccidophaga pada Agroekosistem kacang panjang (Vigna sinensis (L.) telah dilaksanakan pada Bulan April–November 2011 di Desa Haruman Kecamatan Leles Kabupaten Garut. Penelitian dilakukan menggunakan metode survei dengan mengoleksi serangga secara langsung, dan menggunakan berbagai perangkap antara lain perangkap papan kuning, fitfall trap, dan jaring ayun. Serangga hama dominan yang ditemukan adalah Aphis craccivora dengan persentase serangan berkisar 20% - 90%. Jenis aphidophaga dan coccidophaga lain yang ditemukan yaitu dari Ordo Coleoptera : Famili Coccinellidae: Menochilus sexmaculatus, Micraspis sp., Harmonia sp., Verania lineata, Curinus coeruleus, Scymnus sp., Coccinella transversalis; Ordo Diptera : Famili Syrphidae yaitu Ischiodon scutellaris; Neuroptera : Famili Hemerobiidae; Ordo Diptera : Famili Cecidomyiidae Aphidoletes aphidimyza; serta terdapat pula kumbang Carabidae dari Ordo Coleoptera dengan indeks keragaman spesies yang masih tergolong rendah. Kelimpahan tertinggi predator dari kelompok aphidophaga dan coccidophaga didominasi oleh spesies Ischiodon scutellaris dan Menochilus sexmaculatus.Kata kunci : Keragaman, Kelimpahan, Spesies dominan, Serangga hama
Bioaktivitas Formulasi Minyak Biji Azadirachta indica (A. Juss) terhadap Spodoptera litura F. Raden Arif Malik Ramadhan; Lindung Tri Puspasari; Rika Meliansyah; Rani Maharani; Yusup Hidayat; Danar Dono
Agrikultura Vol 27, No 1 (2016): April, 2016
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (475.974 KB) | DOI: 10.24198/agrikultura.v27i1.8470

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ABSTRACTBioactivity Formulation of Seed Neem Oil Azadirachta indica (A.Juss) against Spodoptera litura (F)The purpose of this research was to know the influence of neem seed extract formulation (Azadin 50 EC) on the mortality, larvae development, larvae weight, and food consumption of Armyworm (Spodoptera litura). This research used randomized complete design with 6 treatments and 5 replications, i.e. control, formula at concentration of 0.2%, 0.4%; 0.8%; 1.6%; and 3.2%. Correlation of concentration neem seed oil formulation and mortality of test insect was analysed using probit analysis, weight of test larvae presented in mean and standard deviation, and development time and food consumption of test larvae analysed with analysis of varians. Formula Azadin 50 EC had LC50 value of 0.659% (0.550-0.781%) at 12 days after treatment. The mortality increased significantly in pupae stage that caused LC50 value become 0.152%. The formula prolonged development time, decrease the weight of test insect and decrease food consumption by the larvae.Keywords: Lethal concentration, Mortality, Growth derangement, ExtractABSTRAKPenelitian ini bertujuan untuk mempelajari perlakuan formulasi minyak biji mimba Azadin 50 EC terhadap mortalitas, perkembangan larva, bobot larva dan konsumsi pakan ulat grayak (Spodoptera litura F.). Metode Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan dengan 5 kali ulangan. Perlakuan tersebut yaitu : Kontrol, konsentrasi formula minyak mimba Azadin 50 EC 0,2%; 0,4%; 0,8%; 1,6% dan 3,2%. Hubungan mortalitas dengan konsentrasi formula dianalisis menggunakan analisis probit, sedangkan data bobot larva, konsumsi pakan dan waktu perkembangan larva dianalisis dengan sidik ragam. Hasil penelitian menunjukkan bahwa formula minyak biji A. Indica memiliki nilai LC50 sebesar 0,659% (0,550-0,781%) terhadap larva instar 2 hingga instar 4. Pada fase pupa kematian kembali meningkat tajam sehingga nilai LC50 menjadi 0,152%. Perlakuan formula tersebut mengakibatkan perpanjangan waktu perkembangan, menurunkan bobot, dan menurunkan konsumsi pakan larva uji.Kata Kunci: Konsentrasi letal, Mortalitas, Gangguan perkembangan, Ekstrak
Pengaruh Penambahan Beberapa Esens Buah pada Perangkap Metil Eugenol terhadap Ketertarikan Lalat Buah Bactrocera dorsalis Kompleks pada Pertanaman Mangga di Desa Pasirmuncang, Majalengka Agus Susanto; Wahyu Daradjat Natawigena; Lindung Tri Puspasari; Neng Inne Nur Atami
Jurnal Perlindungan Tanaman Indonesia Vol 22, No 2 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.27001

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Fruit fly (Bactrocera dorsalis Complex) is one of the major pests in horticultural commodities in Indonesia. In this present study, a control method of formulating methyl eugenol and the addition of fruit essences was tested to attract B. dorsalis Complex. The objective was to find out the effect of fruit essences addition in the performance of methyl eugenol in attracting fruit flies and to obtain the most effective fruit essences for attracting male and female fruit flies. The experiment was conducted on mango plantation in Pasirmuncang village, Majalengka, West Java from March 2016 until December 2016. Several synthetic fruit essences which were separately added to methyl eugenol in this experiment were mango, citrus, guava, and starfruit essences. The results showed that the addition of fruit essences on methyl eugenol traps had the same effectiveness  or as good as  any single methyl eugenol trap. Moreover, the additions of mango and orange essences  were not effective enough in attracting female fruit flies, although the number of female fruit flies that were caught were more than the other treatments. IntisariLalat buah Bactrocera dorsalis Kompleks merupakan salah satu hama utama pada komoditas hortikultura. Pada penelitian ini, metode pengendalian dengan memformulasikan metil eugenol dan tambahan esens buah diuji untuk menarik B. dorsalis Kompleks. Tujuannya adalah untuk mengetahui efek penambahan esens buah pada kinerja perangkap metil eugenol dalam menarik lalat buah serta untuk mendapatkan esens buah yang efektif untuk menarik lalat buah betina. Penelitian ini dilakukan di perkebunan mangga di desa Pasirmuncang, Majalengka, Jawa Barat dari bulan Maret 2016 hingga bulan Desember 2016. Beberapa jenis esens buah sintetik yang secara terpisah ditambahkan pada metil eugenol pada percobaan ini adalah mangga, jeruk, jambu biji, dan belimbing. Hasil penelitian menunjukkan bahwa penambahan esens buah pada perangkap metil eugenol memiliki keefektifan yang sama baiknya dengan perangkap metil eugenol secara tunggal. Selain itu, penambahan esens mangga dan esens jeruk belum cukup efektif dalam menarik lalat buah betina meskipun jumlah lalat buah betina yang tertangkap lebih banyak dibandingkan dengan perlakuan lainnya.