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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Biogenesis: Jurnal Ilmiah Biologi Jurnal Pembangunan dan Alam Lestari AGRIVITA, Journal of Agricultural Science Panrita Abdi - Jurnal Pengabdian pada Masyarakat BIOPROSPEK Jurnal Sains Teknologi dan Lingkungan (JSTL) Jurnal SOLMA Jurnal Biologi Tropis AGRIPEAT Asian Journal of Agriculture Jurnal Hutan Tropika BiosciED: Journal of Biological Science and Education (Journal of Environmental Sustainability Management) Biodiversitas Journal of Biological Diversity
Budiman
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Mulawarman. Jl. Barong Tongkok No. 4, Samarinda 75119, East Kalimantan, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Education Engineering Environmental Science Immunology & microbiology Public Health Veterinary Other

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Kelimpahan Mikroplastik dalam Air Minum Isi Ulang: Efek Temperatur Perebusan Nabilah Zafirah Azzahra; Siti Maisaroh; Budiman Budiman; Fatmawati Patang
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 12 No. 2 (2026): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v12i2.890

Abstract

Microplastic contamination in refill drinking water is a relevant public health concern because this product is widely consumed. This study analyzed the effect of boiling temperature on microplastic abundance and characteristics in refill drinking water from Samarinda City. Four samples from different refill depots were randomly collected and treated as unboiled water, water boiled at 70°C, and water boiled at 100°C. Microplastics were separated by density separation using saturated NaCl, organic matter digestion with 30% H2O2, filtration, and microscopic identification. The results showed that refill drinking water contained pellets, fragments, films, and fibers, with black particles being dominant at 58.87%. Boiling at 70°C produced the lowest microplastic abundance and reduced particles by up to 50% compared with unboiled water. In contrast, boiling at 100°C left higher abundance and generated film particles of 20 particles/L. These findings indicate that moderate-temperature boiling is more effective for reducing microplastic exposure before water consumption
Phytoremediation potentials of eight plant species on the tropical gold mine reclamation site in North Sumatra, Indonesia SYAIFUL ANWAR; ASYRAF BIN MANSOR; HAZZEMAN HARIS; QESHY NATA HAYATI; NIR FATHIYA; BUDIMAN BUDIMAN
Asian Journal of Agriculture Vol. 10 No. 1 (2026)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/asianjagric/g100148

Abstract

Abstract. Anwar S, Mansor AB, Haris H, Hayati QN, Fathiya N, Budiman. 2026. Phytoremediation potentials of eight plant species on the tropical gold mine reclamation site in North Sumatra, Indonesia. Asian J Agric 10 (1): g100148. https://doi.org/10.13057/asianjagric/g100148. Post-mining landscapes are often characterized by degraded soils and elevated concentrations of metals, which may hinder ecological recovery and vegetation establishment. This study assessed the accumulation and translocation of metals, i.e., ferrum (Fe), manganese (Mn), zinc (Zn), copper (Cu), nickel (Ni), and aluminium (Al), in eight fast-growing plant species (i.e., Falcataria falcata, Hibiscus tiliaceus, Ixonanthes reticulata, Macaranga conifera, Macaranga tanarius, Melaleuca cajuputi, Melastoma malabathricum, and Samanea saman) at the Martabe gold mining reclamation site, North Sumatra, Indonesia. Soil and plant samples were collected from three reclaimed areas and analyzed using Atomic Absorption Spectrophotometry (AAS). Phytoremediation potentials were assessed using bioconcentration factors (BCF), translocation factors (TF), and phytomining potentials. The results showed that reclaimed soils were acidic and characterized by very high Al concentration (mean 110,100 mg kg-¹) and Fe concentrations (mean 83,309 mg kg-¹) and moderate concentrations of Mn (mean 653 mg kg-¹), Zn (mean 410 mg kg-¹), Cu (mean 277 mg kg-¹), and Ni (mean 513 mg kg-¹). Five planted species showed medium Mn accumulation (BCF 0.1-1), low Zn accumulation in all planted species (BCF 0.01-0.1) and low and negligible uptake in eight species of Fe, Cu, Ni, and Al. Translocation factors indicated Zn and Cu were preferentially translocated to shoots (TF>1), supporting phytoextraction, while Mn exhibited mixed translocation, with M. conifera acting as a phytostabilizer. Leguminous species, particularly I. reticulata, M. cajuputi, and S. saman, demonstrated relatively higher root-to-shoot translocation efficiencies. Preliminary phytomining estimates, based on single-plot biomass measurements, indicated a greater potential removal for Al (up to 13.7 kg ha-¹), Mn (up to 5.1 kg ha-¹), and Fe (up to 3.15 kg ha-¹) compared to other metals. The results demonstrate clear functional differentiation among planted species, and highlight the importance of trait-based species selection and mixed-species plantings to optimize phytoremediation and support revegetation on reclaimed gold mining lands.
Comparison of rhizobacterial communities between secondary forest and palm oil plantations in East Kalimantan, Indonesia ERVINDA YULIATIN; NOVA HARIANI; BODHI DHARMA; FATMAWATI PATANG; BUDIMAN BUDIMAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 1 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260148

Abstract

Abstract. Yuliatin E, Hariani N, Dharma B, Patang F, Budiman. 2025. Comparison of rhizobacterial communities between secondary forest and palm oil plantations in East Kalimantan, Indonesia. Biodiversitas 26: 490-499. The conversion of primary forests to plantations is considered a sustainable form of land management; however, its implications for soil rhizobacterial diversity remain insufficiently explored. This study compared the rhizobacterial communities associated with Palm oil Plantation (PP) and Secondary Forest (SF) in East Kalimantan using a metagenomic approach. The soil samples were collected around the Secondary Forest soil (SF) and Palm oil Plantation soil (PP) plant roots in Berambai, Samarinda. The samples were then analyzed for soil physico-chemical properties such as pH, nitrogen (N), phosphorus (P), potassium (K), organic matter, and C/N ratio. At the same time, the rhizobacterial diversity was analyzed using a metagenomic analysis through Illumina Hiseq. Soil physico-chemical assessments showed acidic conditions in both soils, with PP being more acidic (pH 4.41) than SF (pH 5.38); nutrient analysis indicated medium nitrogen levels and high organic carbon in both soils, while PP had elevated P content due to fertilization. Metagenomic analysis revealed similar rhizobacterial richness, but diversity was slightly higher in PP. Dominant phyla included Proteobacteria and Acidobacteriota, with notable orders like Rhizobiales and Acidobacteriales. The functional analysis highlighted rhizobacterial roles in organic decomposition, plant growth promotion, and nitrogen fixation, illustrating ecological adaptation to soil conditions and management practices. This study provides insights into the rhizobacterial functional diversity in distinct soil environments.
Bioprospecting of Bacillus species from East Kalimantan, Indonesia for developing sustainable mosquito larvicides NOVA HARIANI; BUDIMAN BUDIMAN; FATMAWATI PATANG; ERVINDA YULIATIN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 3 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260336

Abstract

Abstract. Hariani N, Budiman, Patang F, Yuliatin E. 2025. Bioprospecting of Bacillus species from East Kalimantan, Indonesia for developing sustainable mosquito larvicides. Biodiversitas 26: 1360-1366. Climate change, unplanned urbanization, environmental pollution, and the development of insecticide-resistant mosquito populations have increased Vector-Borne Disease (VBD) incidents in tropical and subtropical countries. They require an innovative and sustainable action strategy. As the endemic area of VBDs, East Kalimantan stores unexplored biocontrol agents such as Bacillus species. Therefore, this study tested the toxicity of nine single and three mixed isolates of Bacillus from East Kalimantan against mosquito larvae of both Aedes aegypti and non-Ae. aegypti species; and identified the selected Bacillus isolates using a 16S rDNA sequence approach. Five single isolates of Bacillus from East Kalimantan (BP1, BP2, BP3, BF2, and BF3) and their consortia (BPM, BFM, and BFP) decreased the larvae number over time. They demonstrated high effectiveness in reducing Ae. aegypti and non-Ae. aegypti larvae population with more than 50% mortality at 48 hours of exposure. However, utilizing a single isolate of Bacillus as a biocontrol agent against mosquitoes was preferred over using mixed isolates for efficiency in larvacide production, offering a sustainable alternative to chemical insecticides. Moreover, Bacillus-BP3 and Bacillus-BF2 isolates from East Kalimantan, identified as Bacillus subtilis, were strong and safe candidates for developing sustainable larvicides to control mosquito populations with larvae mortality percentage up to 100% in 24 hours, mainly Ae. aegypti. Other isolates were identified as Bacillus cereus (BP1) and Bacillus tropicus (BP2), which also showed high and consistent larvacide activity from the beginning of the exposure.
Plant diversity and vegetation structure across reclamation ages in a tropical gold mine landscape, North Sumatra, Indonesia SYAIFUL ANWAR; ASYRAF BIN MANSOR; HAZZEMAN HARIS; RIZKY WAHYUDI; QESHY NATA HAYATI; BUDIMAN BUDIMAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 12 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261209

Abstract

Abstract. Anwar S, Mansor AB, Haris H, Wahyudi R, Hayati QN, Budiman. 2025. Plant diversity and vegetation structure across reclamation ages in a tropical gold mine landscape, North Sumatra, Indonesia. Biodiversitas 26: 6056-6073. Restoration is essential to recover biodiversity in post-mining landscapes. This study aimed to descriptively compare the plant diversity and vegetation structure in reclamation sites aged 3 to 12 years at the Martabe gold mine, North Sumatra, Indonesia. Vegetation was sampled using nested quadrat methods with 10% sampling intensity (2.36 ha of 22.8 ha total area); species richness (S), Shannon–Wiener diversity (H?), Margalef richness (R), and evenness (E) were calculated. The Morisita index quantified community similarity, and species dominance was assessed using the Importance Value Index (IVI). Density profiles (ind·ha-¹) were also constructed to examine stand structure. This research provides novel insights into the trajectories of ecological succession and restoration potential in tropical mining landscapes. Across all age groups, 177 species were recorded, with seedling-stage richness peaking at 58 species in 3-year-old sites. Seedling communities showed high diversity but low evenness, indicating pioneer species dominance. Sapling and pole stages exhibited moderate diversity with balanced species distributions, while tree communities had the lowest richness but increasing evenness with age. Community similarity increased from the seedling to the tree stage, suggesting convergence toward a mature assemblage. Falcataria falcata dominated the tree layer, while Samanea saman and Hibiscus tiliaceus prevailed in sapling and pole layers. Pioneer plants like Melastoma malabathricum and Asystasia gangetica were common among the young seedlings. The vegetation density displayed an inverted J-shaped pattern, indicating healthy regrowth. These findings reveal changes in species composition, dominance, and structure over a decade of reclamation, providing valuable information to enhance mine-land restoration strategies in tropical regions.
Spatial Variation in Water Quality Based on Biotic Indices Along Urban River Reservoir Continuum in Samarinda Budiman Budiman; Khairunnisa Luthfiah; Fatmawati Patang
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11509

Abstract

The Karang Mumus River and Benanga Reservoir are water bodies that play a vital role in supporting ecological functions and meeting the socio-economic needs of urban dwellers of Samarinda. However, pressure from urban anthropogenic activities has caused a decline in water quality in both water bodies. This study aims to assess water quality along the Benanga Reservoir and Karang Mumus River using benthic macroinvertebrates as ecological indicators. Macroinvertebrate benthos sampling was conducted in March 2024 in the upper, middle, and lower reaches of the Karang Mumus River, as well as the Benanga Reservoir, which is the source of the river's water flow. Furthermore, water quality assessment along these waters was determined based on the importance value index (INP), Shannon-Wiener diversity (H'), evenness (E), and Simpson dominance (D), as well as biotic indices, namely BMWP-ASPT (Biological Monitoring Working Party-Average Score Per Taxon) and FBI (Family Biotic Index). The results of the study showed that Benanga Reservoir has a fairly high diversity (H' = 2.05), with a dominance of taxa sensitive to pollution, such as the Baetidae family, indicating relatively good water conditions. BMWP-ASPT and FBI values ​​indicate that the quality of Benanga Reservoir is relatively good. In contrast, the upstream, middle, and downstream segments of the Karang Mumus River showed a gradual decline in water quality. In the middle and downstream segments, the dominance of taxa tolerant of aquatic environmental pollution, such as Lestidae and Tubificidae, was observed, along with a high FBI value (9.49), indicating that the water is highly polluted with organic materials.
Aklimatisasi Anggrek Spesies Hasil Kultur Jaringan melalui Pemberdayaan Mahasiswa Tingkat Akhir Program Studi Biologi Universitas Mulawarman Samsurianto; Ratna Kusuma; Yanti Puspita Sari; Dwi Susanto; Hetty Manurung; Bodhi Dharma; Eko Kusumawati; Budiman; Jusmaldi; Ervinda Yuliatin; Aloysia Sri Pujiyanti; Febry Rahmadhani Hasibuan
Panrita Abdi - Jurnal Pengabdian pada Masyarakat Vol. 10 No. 3 (2026): Jurnal Panrita Abdi - Juli 2026
Publisher : LP2M Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/pa.v10i3.48712

Abstract

Student empowerment through the acclimatization of tissue culture–derived orchids represents a strategic approach that integrates biodiversity conservation efforts with the enhancement of human resource capacity. This community service program aimed to improve the knowledge and skills of final-year Biology students in acclimatizing two orchid species of high conservation and economic value, namely Coelogyne pandurata and Phalaenopsis gigantea. The program employed a combination of theoretical instruction, demonstrations of acclimatization techniques, hands-on training, and interactive discussions on business opportunities in orchid cultivation. A total of 15 students participated in the program, which was preceded by a pre-test and concluded with a post-test to assess improvements in participants’ understanding. The evaluation results showed a significant increase in post-test scores (85.33%) compared to pre-test scores (58.67%), with an average N-Gain value of 0.65, classified as moderate. In addition, participants were able to independently perform all stages of the acclimatization process, including the removal of plantlets from culture bottles, washing off residual media, planting in sterile substrates, and the covering process. Questionnaire results indicated a high level of participant satisfaction with the materials, methods, and overall benefits of the program. Overall, this community service activity successfully enhanced students’ knowledge and technical skills in the acclimatization of tissue culture–derived orchids, supported the conservation of high-value orchid species, and strengthened students’ capacity in horticulture and bioresource-based entrepreneurial development. Pemberdayaan mahasiswa melalui kegiatan aklimatisasi anggrek hasil kultur jaringan merupakan pendekatan strategis yang mengintegrasikan upaya konservasi keanekaragaman hayati dengan peningkatan kapasitas sumber daya manusia. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan mahasiswa tingkat akhir Program Studi Biologi dalam melakukan aklimatisasi dua spesies anggrek bernilai konservasi dan ekonomi tinggi, yaitu Coelogyne pandurata dan Phalaenopsis gigantea. Metode pelaksanaan kegiatan meliputi pemberian materi teoritis, demonstrasi teknik aklimatisasi, praktik langsung (hands-on training), serta diskusi interaktif terkait peluang usaha budidaya anggrek. Kegiatan diikuti oleh 15 mahasiswa dan diawali dengan pre-test serta diakhiri dengan post-test untuk mengukur peningkatan pemahaman peserta. Hasil evaluasi menunjukkan adanya peningkatan signifikan nilai post-test sebesar 85,33% dibandingkan nilai pre-test sebesar 58,67%, dengan nilai rata-rata N-Gain sebesar 0,65 yang tergolong dalam kategori sedang. Selain itu, peserta mampu melaksanakan seluruh tahapan aklimatisasi secara mandiri, mulai dari pelepasan planlet dari botol kultur, pencucian sisa media, penanaman pada media steril, hingga proses penyungkupan. Hasil angket menunjukkan tingkat kepuasan peserta yang tinggi terhadap materi, metode, dan manfaat kegiatan. Secara keseluruhan, kegiatan pengabdian ini berhasil meningkatkan kompetensi pengetahuan dan keterampilan mahasiswa dalam aklimatisasi anggrek hasil kultur jaringan, mendukung upaya konservasi anggrek bernilai tinggi, serta memperkuat kapasitas mahasiswa dalam bidang hortikultura dan pengembangan kewirausahaan berbasis sumber daya hayati.
Co-Authors Aloysia Sri Pujiyanti Annida Nurul F. A., Annisa Nurul Ilmi dan Fauzan Ibram M. Arisoesiloningsih, Endang ASYRAF BIN MANSOR ASYRAF BIN MANSOR BODHI DHARMA Bodhi Dharma Dina Hayati Putri Dwi Susanto Edi Wibowo, Ratno Bagus Eko Kusumawati Endang Arisoesilaningsih ERVINDA YULIATIN Ervinda Yuliatin FATMAWATI PATANG Fatmawati Patang Fatmawati Patang Febry Rahmadhani Hasibuan Febry Rahmadhani Hasibuan Fidelis Kristianto Fudiantoro, Aldi Handiargo, Ferdy HAZZEMAN HARIS HAZZEMAN HARIS Hetty Manurung Jusmaldi Jusmaldi Khairunnisa Luthfiah Kurniati, Reni Nabilah Zafirah Azzahra NIR FATHIYA NOVA HARIANI Nova Hariani Nurmianti, Nurmianti Nurmianti, Nurmianti QESHY NATA HAYATI QESHY NATA HAYATI Ratna Kusuma RIZKY WAHYUDI Samsurianto Siti Maisaroh Sumarso Sumarso Sutra, Anang SYAIFUL ANWAR SYAIFUL ANWAR Yanti Puspita Sari Yuliatin, Ervinda