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All Journal Jurnal Pelayanan dan Pengabdian Masyarakat Indonesia Jurnal Ilmu Kesehatan dan Gizi Central Publisher Jurnal Pengabdian Masyarakat Indonesia Sejahtera Jurnal Intelek Insan Cendikia Ekspresi: Publikasi Kegiatan Pengabdian Indonesia Panggung Kebaikan : Jurnal Pengabdian Sosial JURNAL TEKNOLOGI, KESEHATAN DAN ILMU SOSIAL Klinik: Jurnal Ilmiah Kedokteran dan Kesehatan
Manuppak Irianto Tampubolon
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Identifikasi Bakteri dan Jamur pada Makanan dan Minuman Menggunakan Metode Polymerase Chain Reaction (PCR) dan Analisis Deoxyribonucleic Acid (DNA) Molekuler Devina Chandra; Manuppak Irianto Tampubolon; Monica Suryani; Supartiningsih Supartiningsih
Jurnal Ilmiah Kedokteran dan Kesehatan Vol. 5 No. 3 (2026): September: Jurnal Ilmiah Kedokteran dan Kesehatan
Publisher : Lembaga Pengembangan Kinerja Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/klinik.v5i3.7660

Abstract

Contamination of food and beverages by pathogenic bacteria and spoilage fungi remains a major public health concern, particularly in traditional and street-vended products where hygienic control is often inadequate. Conventional culture-based identification methods are time-consuming and often fail to detect slow-growing or non-culturable microorganisms. This study aimed to identify bacterial and fungal contaminants in food and beverage samples using Polymerase Chain Reaction (PCR) combined with molecular Deoxyribonucleic Acid (DNA) analysis. Samples were collected from local food vendors, and total genomic DNA was extracted using a commercial extraction kit. Bacterial identification targeted the 16S rRNA gene, while fungal identification targeted the Internal Transcribed Spacer (ITS) region, both amplified by PCR and visualized by agarose gel electrophoresis. Amplicons were sequenced and compared with reference sequences in the GenBank database using the Basic Local Alignment Search Tool (BLAST). The results revealed the presence of pathogenic and spoilage microorganisms, including Escherichia coli, Staphylococcus aureus, Salmonella sp., and Aspergillus species, indicating inadequate sanitation during food handling and storage. These findings confirm that PCR-based molecular analysis provides a rapid, sensitive, and specific approach for detecting microbial contamination in food and beverages.
Co-Authors Adinda Nur'Sabilla Alfi Sapitri Alfian Rejekinta Munthe Catur Rafia Ningrum Cut Masyitah Thaib Dedi Maruli Tua Napitupulu Desni Rinanda Silitonga Devi Rahmayuni Br Harahap Devina Chandra Dhea Nur Fadhilah Dumartina Hutauruk Eva Diansari Marbun Febriana Rianti Br Siagian Maria Pratiwi Manik Monica Suryani Natanael Priltius Sindy Yupani Br Sembiring Steven Tandiono Supartiningsih - - Widya Astuti Wiwin Katarina Sihotang Yossi Rodearni Br Manurung