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Machine learning for potential anti-cancer discovery from black sea cucumbers Fahrury Romdendine, Muhammad; Fatriani, Rizka; Ananta Kusuma, Wisnu; Annisa, Annisa; Nurilmala, Mala
IAES International Journal of Artificial Intelligence (IJ-AI) Vol 13, No 3: September 2024
Publisher : Institute of Advanced Engineering and Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11591/ijai.v13.i3.pp3157-3163

Despite being an abundant marine organism in Indonesia, black sea cucumbers (Holothuria atra) is still underutilised due to its slightly bitter taste. This study aims to identify potential anti-cancer compounds from black sea cucumbers using machine learning (ML) to perform drug discovery. ML models were used to predict interactions between compounds from the organism with cancer-related proteins. Following prediction, all compounds were computationally validated through molecular docking. The validated compounds were then screened using absorption, distribution, metabolism, excretion, and toxicity (ADMET) Lab 2.0 to assess their druglike properties. The results showed that ML predicted seven out of 86 compounds were interacted with cancer-related proteins. Computational validation from the results showed that four out of seven compounds demonstrated stable interaction with proteins where only one compound meet the criteria of drug-like compound. The framework of ML and computational validation highlighted in this study shows a great promise in the future of drug discovery specifically for marine organisms. Since computational method only works in prediction realms, wet lab validation and clinical trials are imperative before the drug candidate can be produced as actual anti-cancer drug.

Pelatihan Pembuatan Pupuk Kompos di Desa Ngestirahayu dalam Upaya Kemandirian Pertanian Maretta, Gres; Istiadi, Khaerunissa Anbar; Wibawa, Fajri Arif; Budiono, Desi; Fatriani, Rizka; Hariyandi, Yopi; Astuti, Ayu Widia; Ferdinanda, Thierry; Aniesti, Frigia Rafilia
Lumbung Inovasi: Jurnal Pengabdian kepada Masyarakat Vol. 9 No. 4 (2024): December
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/linov.v9i4.2355

Desa Ngestirahayu merupakan salah satu Desa yang terletak di Kecamatan Punggur dengan mayoritas masyarakat Desa Ngestirahayu berprofesi sebagai petani. Selama ini, petani mengalami permasalahan salah satunya adalah ketersediaan dan supply pupuk subsidi yang dibatasi. Oleh sebab itu, diperlukan alternatif pupuk yang murah dan dapat diproduksi secara mandiri. Selain itu, pupuk organik dapat memperbaiki kualitas tanah dan menyediakan nutrisi esensial bagi tanaman secara lebih berkelanjutan. Pupuk organik dapat dibuat dengan menggunakan alat dan bahan yang sederhana dan murah. Namun masyarakat desa umumnya masih belum familiar dan memahami mengenai teknik pembuatan pupuk organik. Berdasarkan hal tersebut, maka perlu dilakukan pelatihan pembuatan pupuk organik pada KWT di desa Ngestirahayu. Hasil akhir dari pelaksanaan kegiatan adalah terjadinya peningkatan pengetahuan masyarakat sebesar 91,6% mengenai pupuk, bahan, proses pembuatan pupuk organik. Manfaat kegiatan ini yaitu meningkatkan pengetahuan mitra mengenai penggunaan pupuk organik, mengetahui bahan yang digunakan dan tatacara pembuatan pupuk organik. Masyarakat perlu dilakukan pembiasaan untuk mulai memilah sampah berdasarkan jenisnya mulai dari skala rumah tangga, sehingga memudahkan untuk mengolah sampah menjadi pupuk kompos. Selain itu, perlu dilakukan produksi pupuk kompos dalam jumlah banyak untuk mencukupi kebutuhan pupuk desa, sebagai kegiatan lanjutan dari program ini. Compost Fertilizer Production Training in Ngestirahayu Village to Achieve Agricultural Independence Abstract The village of Ngestirahayu is one of the villages located in the Punggur District, where the majority of the residents work as farmers. Farmers have long faced issues, one of which is the limited availability and supply of subsidized fertilizers. Therefore, there is a need for an affordable and self-produced fertilizer alternative. In addition, organic fertilizers can improve soil quality and provide essential nutrients for plants in a more sustainable manner. Organic fertilizers can be made using simple and inexpensive tools and materials. However, the village community is generally not yet familiar with or knowledgeable about organic fertilizer production techniques. Based on this, it is necessary to conduct training on organic fertilizer production for the Women's Farming Group (KWT) in the village of Ngestirahayu. The final outcome of this activity was a 91.6% increase in community knowledge regarding fertilizers, materials, and the process of making organic fertilizers. The benefits of this activity include enhancing partners' knowledge of organic fertilizer use, understanding the materials used, and learning the process of making organic fertilizers. The community needs to develop a habit of sorting waste by type at the household level, making it easier to process waste into compost. Furthermore, large-scale compost production is needed to meet the village's fertilizer needs as a follow-up activity of this program.

Molecular Docking Study of Bioactive Compounds from Curcuma aeruginosa Roxb. as Antioxidant and Anticancer Activities Ambarsari, Laksmi; Nurjanah, Siti; Artika, I Made; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.327

Cancer is one type of dangerous disease that is increasing every year. Free radicals are the cause of gene mutations (cancer). Cancer will develop uncontrollably due to the occurrence of the cell cycle and the presence of compounds that trigger cell proliferation and inhibit the process of apoptosis. This cancer treatment is carried out by giving cyclin-dependent kinase2 (CDK2) and cyclooxygenase-2 (COX-2) inhibitor drugs to inhibit cancer development, as well as lipoxygenase (LOX) inhibitor drugs for the formation of free radicals. Curcuma aeruginosa RoxB., the plant, is known to have the potential for antioxidant and anticancer properties. This study aims to determine the molecular interaction between the dominant compound in the ethanol extract of C. aeruginosa with CDK2, COX-2, and LOX receptors. The type of ligand interaction with the receptor was determined through the parameters of affinity energy (∆G), inhibition constant (Ki), type of interaction, and percentage of binding site similarity (%BSS). The results showed that the gajutsulacton A had the best potential in inhibiting CDK2. The cucumenol may be a COX-2 inhibitor, and there are no compounds that can inhibit LOX as well as an antioxidant. Thus, our findings demonstrate the potential for C. aeruginosa bioactive to serve as anticancer candidate molecules against CDK2 and COX-2 receptors.

Phytochemical Screening, Cytotoxicity Test, and Antibacterial Activity Test of Wungu Plant Extract (Graptophyllum pictum L. Griff) Arista, Rini Anggi; Jalloh, Mohammed A.; Insani, Fahma Alifia; Balqis, Putri Azzahara; Larasati, Cindy; Arief, Barieq Farial Putra; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.330

Indonesia boasts a rich array of medicinal herbs, including the wungu (Graptophyllum pictum L. Griff), which is known for its potential as an anticancer and antibacterial agent. Here, we used qualitative phytochemical assays to identify bioactive compounds in various parts of the wungu plant (leaves, stem, and roots) and their efficacy in combating cancer and bacterial infections. The brine shrimp lethality test (BSLT) was used to measure anticancer properties and antibacterial activity against Escherichia coli. We found that the leaves contained flavonoids, triterpenoids, phenols, saponins, and alkaloids, whereas the stem and root contained steroids and alkaloids. Notably, the wungu plant exhibited no toxicity, as shown by the cytotoxicity test, indicating a lack of anticancer potential as all parts showed LC50 values exceeding 1000 ppm. However, the antibacterial assays strikingly showed efficacy against bacteria across all parts of the plants, with varying strengths from weak to strong. This study sheds light on the promising antibacterial properties of the wungu plant and its potential as a therapeutic agent.

Kepadatan dan Keanekaragaman Avifauna di Taman Hutan Raya (Tahura) Banten: Analisis dengan Metode Point Count Haryandi, Yopi; Fatriani, Rizka; Nugraheni, Latif Sofiana; Mukhoyyaroh, Qiswatun; Utami, Noviani
MAXIMUS: Journal of Biological and Life Sciences Vol. 3 No. 1 (2025)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v3i1.2148

Birds play a crucial ecological role in maintaining forest ecosystem balance. However, environmental changes and anthropogenic pressures can affect bird population density and diversity in a given area. This study aims to identify the population density and diversity of birds in Banten Grand Forest Park. Data collection was conducted on June 22–23, 2024, using the Point Count method at five observation points within the Tahura Banten area. Observations were carried out in the morning (06:00–10:00 WIB) and in the afternoon (15:00–18:00 WIB). Population density was analyzed using the formula D = N/A, while bird diversity was assessed using the Shannon-Wiener Index. The results recorded 95 individual birds, classified into 25 species and 16 families. The Black-capped Babbler (Pellorneum capistratum) and the Common Iora (Aegithina tiphia) were the species with the highest density, reaching 2.55 individuals per hectare. The Shannon-Wiener Index calculation yielded a value of H’ = 3.03, indicating a high level of species diversity in the study area. These findings suggest that Tahura Banten remains an important habitat for various bird species. This study highlights the importance of forest ecosystem conservation in Banten Grand Forest Park to ensure the sustainability of bird populations and biodiversity in the region. Keywords: Population density, bird diversity, Point Count, Tahura Banten, conservation

Caffeine Molecular Target Identification and Protein Interaction Analysis in Alzheimer's Disease Fatriani, Rizka; Haryandi, Yopi; Khairani, Iffa Afiqa; Anisa, Hida Arliani Nur
MAXIMUS: Journal of Biological and Life Sciences Vol. 3 No. 1 (2025)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LPPM), Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35472/maximus.v3i1.2143

Caffeine is known to have various biological effects, including its potential to modulate the central nervous system. This study aims to identify the molecular targets of caffeine and explore their relationship with Alzheimer's disease using a bioinformatics approach. The methods used include target prediction using SwissTargetPrediction and SuperPred, target relationship analysis with disease through the DAVID database, protein interaction exploration and enrichment analysis using STRING-DB, and network analysis using Cytoscape. The results showed that from the total targets obtained, there were 20 overlapping targets related to Alzheimer's. Protein interaction analysis revealed 17 nodes with 32 significant interactions, which provide insight into the molecular pathways that caffeine can manipulate in the context of neuroprotection. These findings are in line with various studies showing that caffeine consumption is associated with a reduced risk of Alzheimer's through modulation of the nervous system and inflammation.

Molecular Insights into the Therapeutic Potential of Curcuma amada Rhizome: A Network Pharmacology and Docking Approach Fatriani, Rizka; Istiadi, Khaerunissa Anbar; Khairani, Iffa Afiqa
Jurnal Jamu Indonesia Vol. 10 No. 2 (2025): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v10i2.397

The rhizome of Curcuma amada (ginger mango) has traditionally been used for various medicinal purposes. However, its molecular targets and mechanisms of action are still poorly understood. This study aimed to predict the potential bioactive compounds and target proteins of C. amada using network pharmacology and molecular docking approaches. A total of 110 compounds were identified, and their predicted targets were analyzed through a protein-protein interaction (PPI) network, enrichment, and disease analysis. Key targets include phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (PIK3CA) and tyrosine-protein kinase JAK2 (JAK2), both of which are closely related to cancer-related pathways. Four compounds, β-eudesmol, (E,Z)-farnesol, spathulenol, and τ-muurolol, were selected for molecular docking studies. Validation of the docking protocol through re-docking showed low RMSD values (0.667 Å for PIK3CA and 0.474 Å for JAK2), confirming the reliability of the method. The docking results demonstrated that the native ligands and selected compounds formed multiple hydrogen bonds and extensive hydrophobic interactions with key residues in the active site. Notably, most of the interactions were hydrophobic, which is consistent with the volatile nature of the ligands. The binding affinity was below –5 kcal/mol for all tested compounds. These findings suggest that C. amada rhizomes contain bioactive compounds capable of modulating cancer-related targets, thus providing a molecular basis for their potential therapeutic effects.

Molecular Docking Study of Bioactive Compounds from Curcuma aeruginosa Roxb. as Antioxidant and Anticancer Activities Ambarsari, Laksmi; Nurjanah, Siti; Artika, I Made; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.327

Cancer is one type of dangerous disease that is increasing every year. Free radicals are the cause of gene mutations (cancer). Cancer will develop uncontrollably due to the occurrence of the cell cycle and the presence of compounds that trigger cell proliferation and inhibit the process of apoptosis. This cancer treatment is carried out by giving cyclin-dependent kinase2 (CDK2) and cyclooxygenase-2 (COX-2) inhibitor drugs to inhibit cancer development, as well as lipoxygenase (LOX) inhibitor drugs for the formation of free radicals. Curcuma aeruginosa RoxB., the plant, is known to have the potential for antioxidant and anticancer properties. This study aims to determine the molecular interaction between the dominant compound in the ethanol extract of C. aeruginosa with CDK2, COX-2, and LOX receptors. The type of ligand interaction with the receptor was determined through the parameters of affinity energy (∆G), inhibition constant (Ki), type of interaction, and percentage of binding site similarity (%BSS). The results showed that the gajutsulacton A had the best potential in inhibiting CDK2. The cucumenol may be a COX-2 inhibitor, and there are no compounds that can inhibit LOX as well as an antioxidant. Thus, our findings demonstrate the potential for C. aeruginosa bioactive to serve as anticancer candidate molecules against CDK2 and COX-2 receptors.

Phytochemical Screening, Cytotoxicity Test, and Antibacterial Activity Test of Wungu Plant Extract (Graptophyllum pictum L. Griff) Arista, Rini Anggi; Jalloh, Mohammed A.; Insani, Fahma Alifia; Balqis, Putri Azzahara; Larasati, Cindy; Arief, Barieq Farial Putra; Fatriani, Rizka
Jurnal Jamu Indonesia Vol. 8 No. 2 (2023): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v8i2.330

Indonesia boasts a rich array of medicinal herbs, including the wungu (Graptophyllum pictum L. Griff), which is known for its potential as an anticancer and antibacterial agent. Here, we used qualitative phytochemical assays to identify bioactive compounds in various parts of the wungu plant (leaves, stem, and roots) and their efficacy in combating cancer and bacterial infections. The brine shrimp lethality test (BSLT) was used to measure anticancer properties and antibacterial activity against Escherichia coli. We found that the leaves contained flavonoids, triterpenoids, phenols, saponins, and alkaloids, whereas the stem and root contained steroids and alkaloids. Notably, the wungu plant exhibited no toxicity, as shown by the cytotoxicity test, indicating a lack of anticancer potential as all parts showed LC50 values exceeding 1000 ppm. However, the antibacterial assays strikingly showed efficacy against bacteria across all parts of the plants, with varying strengths from weak to strong. This study sheds light on the promising antibacterial properties of the wungu plant and its potential as a therapeutic agent.

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