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All Journal Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Bioma : Berkala Ilmiah Biologi Biology, Medicine, & Natural Product Chemistry Pharmacon INDONESIAN JOURNAL OF PHARMACY JC-T (Journal Cis-Trans): Jurnal Kimia dan Terapannya MEDIA INFORMASI Fullerene Journal of Chemistry Jurnal Ilmiah Jophus: Journal of Pharmacy UMUS Prosiding University Research Colloquium Sinteza Journal of Pharmacy Science and Technology Parapemikir Jurnal Ilmiah Farmasi CERATA Jurnal Sains dan Kesehatan Jurnal Farmasi Galenika
Wirasti, Wirasti
Prodi Sarjana Farmasi FIKes UMPP
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Penetapan Kadar Logam Berat Kadmium (Cd) dan Timbal (Pb) pada Ikan Kakap Putih, Ikan Kerapu dan Ikan Tigawaja di Perairan Kota Pekalongan Suryani, Irma; Nur, Achmad Vandian; Rahmasari, Khusna Santika; Wirasti, Wirasti
Jurnal Kimia Fullerene Vol 9 No 2 (2024): Fullerene Journal Of Chemistry
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37033/fjc.v9i2.694

Abstract

Kota Pekalongan adalah daerah industri batik yang limbahnya mengandung logam berat berakibat tercemarnya perairan. Pencemaran dapat mempengaruhi kualitas air serta diserap oleh biota perairan sehingga membuat hasil tangkapan ikan tercemar, sehingga jika dikonsumsi oleh manusia menyebabkan toksisitas. Logam kadmium (Cd) dan Timbal (Pb) bersifat karsinogenik, memberikan efek genotoksik, sitotoksik, kerusakan pada paru-paru, ginjal, hati, sistem saraf dan darah. Tujuan dari penelitian ini adalah mengetahui kandungan kadar logam berat kadmium (Cd) dan timbal (Pb) pada ikan kakap putih, ikan kerapu dan ikan tigawaja di Perairan Kota Pekalongan. Metode dalam penelitian ini dilakukan secara kuantitatif dengan menggunakan instrumen AAS (Atomic Absorption Spectrophotometry). Hasil penelitian menunjukkan bahwa kadar kadmium (Cd) dalam ikan kakap putih sebesar 0,17 mg/Kg, ikan kerapu sebesar 0,22 mg/Kg dan ikan tigawaja sebesar 0,19 mg/Kg. Kandungan kadar timbal (Pb) pada ikan kakap putih, ikan kerapu dan ikan tigawaja secara berurutan sebesar 1,64 mg/Kg, 2,63 mg/Kg dan 1,35 mg/Kg. Ketiga sampel kadar kadmiumnya melebihi batas ambang yang ditetapkan oleh BPOM No. 5 Tahun 2018 sebesar 0,10 mg/Kg. Kadar timbal dalam ketiga sampel melebihi batas ambang yang ditetapkan oleh BPOM No. 9 Tahun 2022 sebesar 0,3 mg/Kg.
Uji Efek Ekstrak Etanol Herba Ketumbar (Coriandrum sativum L.) Sebagai Afrodisiak Terhadap Tikus Putih Jantan Galur Wistar (Rattus norvegicus) Wirasti, Wirasti; Anjani, Lestari Dwi; Waznah, Urmatul; Wahyu Permadi, Yulian
JURNAL FARMASI GALENIKA Vol 12 No 2 (2025): Jurnal Farmasi Galenika
Publisher : Universitas Bhakti Kencana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70410/jfg.v12i2.303

Abstract

One of the common male sexual disorders is low libido (sexual desire). An alternative approach to overcome this problem is the use of aphrodisiacs, substances that stimulate and enhance sexual desire. Coriander (Coriandrum sativum L.) contains flavonoids, alkaloids, saponins, and terpenoids, which may improve blood circulation and potentially enhance sexual activity. This study aimed to evaluate the aphrodisiac activity of ethanolic extract of coriander herb (EHC) on the libido of male Wistar rats (Rattus norvegicus). The extract was obtained using the maceration method, while the aphrodisiac test was conducted on 25 male and 25 female rats aged 2–2.5 months with body weight ranging from 150–200 g. The animals were divided into five groups, each consisting of 5 males and 5 females: negative control (1% Na CMC), positive control (sildenafil 50 mg), and three treatment groups receiving EHC at doses of 400 mg/kgBW, 600 mg/kgBW, and 800 mg/kgBW. For five consecutive days (6:00 p.m.–7:00 a.m.), one male and one female were placed in the same cage, and sexual activities of male rats (introducing and mounting) were observed using CCTV recordings. The results showed that EHC exhibited aphrodisiac activity at a dose of 800 mg/kgBW, which was not significantly different from the positive control (sildenafil 50 mg) (p=0.571). Statistical analysis using One-Way ANOVA indicated a significant difference among groups (p<0.05), and Tukey’s post-hoc test confirmed significant differences among all EHC dose groups (p<0.05). In conclusion, the ethanolic extract of coriander herb demonstrates potential as an aphrodisiac to enhance libido in male Wistar rats.
Uji Aktivitas Antioksidan dan Inhibisi Enzim alfa-Amilase Nano Ekstrak Kulit Buah Semangka (Citrullus lanatus (Thunb.) Matsum & Nakai): Antioxidant and alpha-Amylase Inhibitory Activities of Nano Extract From Watermelon Rind (Citrullus Lanatus (Thunb.) Matsum & Nakai) Indriyani, Puspa; Rahmasari, Khusna Santika; Wirasti, Wirasti; Slamet, Slamet
Jurnal Sains dan Kesehatan Vol. 7 No. 6 (2025): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v7i6.2560

Abstract

Watermelon rind (albedo) contains secondary metabolites such as alkaloids, flavonoids, saponins, tannins, as well as vitamins, minerals, lycopene, and citrulline, which have potential as antioxidants and regulators of glucose metabolism. This study aimed to determine the antioxidant activity and ?-amylase enzyme inhibition of watermelon rind extract and nano-extract. The extract was prepared using the maceration method with 96% ethanol, while the nano-extract was produced through ionic gelation. Antioxidant activity was tested using the FRAP method with UV-Vis spectrophotometry, while enzyme inhibition was measured using a microplate reader. The extract yield obtained was 14.30%. Nano characterization showed a transmittance percentage of 97.56 ± 0.05, particle size of 418.63 nm ± 1.06, polydispersity index of 0.622 ± 0.03, and zeta potential of 46.29 mV ± 0.11. The IC50 values for antioxidant activity of the extract and nano-extract were 81.46 µg/mL ± 0.23 and 62.88 µg/mL ± 0.036, respectively, while the IC50 for ?-amylase inhibition was 59.17 µg/mL ± 1.79 and 43.51 µg/mL ± 1.59. The nano-extract was proven to enhance both activities. Keywords:          FRAP, Nano Extract of Watermelon Rind, ?-amylase enzyme   Abstrak Kulit semangka (albedo) mengandung senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin, tanin, serta vitamin, mineral, likopen, dan citrulline yang berpotensi sebagai antioksidan dan pengatur metabolisme glukosa. Penelitian ini bertujuan mengetahui aktivitas antioksidan dan inhibisi enzim ?-amilase dari ekstrak dan nano ekstrak kulit semangka. Ekstrak dibuat dengan metode maserasi menggunakan etanol 96%, sedangkan nano ekstrak melalui gelasi ionik. Aktivitas antioksidan diuji dengan metode FRAP menggunakan spektrofotometri UV-Vis, sedangkan inhibisi enzim diukur dengan microplate reader. Rendemen ekstrak diperoleh 14,30%. Karakterisasi nano menunjukkan % transmitan 97,56 ± 0,05, ukuran partikel 418,63 nm ± 1,06, indeks polidispersitas 0,622 ± 0,03, dan zeta potensial 46,29 mV ± 0,11. Nilai IC50 antioksidan ekstrak dan nano ekstrak masing-masing 81,46 µg/mL ± 0,23 dan 62,88 µg/mL ± 0,036, sedangkan IC50 inhibisi enzim ?-amilase 59,17 µg/mL ± 1,79 dan 43,51 µg/mL ± 1,59. Nano ekstrak terbukti meningkatkan kedua aktivitas tersebut. Kata Kunci:         FRAP, Nano Extract of Watermelon Rind, Enzim ?-Amilase
SINTESIS PREKURSOR SENYAWA 2-AMINOBUTYRIC ACID (ABA) DENGAN STARTING MATERIAL MONOSODIUM GLUTAMATE (MSG) Syarifah, Dewi; Slamet, Slamet; Wirasti, Wirasti; Mugiyanto, Eko
Prosiding University Research Colloquium Proceeding of The 8th University Research Colloquium 2018: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

The aim of the research is to synthesize the compound of Precursor of ABA from MSG the starting material by structural modification as well as to determine the characteristics and physical properties of the synthesis compound. It was done by modification of chemical structure starting with glutamic acid isolation from MSG. Then it isolate reacted with AlCl3 to obtain the ABA compound precursor. The rendemen was 18.5%, of synthesis identification obtained of white powder, odorless, dissolves in hot water and melt at a temperature of 187-189 0C. The UV-Vis spectrophotometry showed that the synthesis process formed a new compound. Confirm with HPLC analysis.
UJI AFRODISIAK EKSTRAK ETANOL BUAH TERUNG UNGU TERHADAP LIBIDO TIKUS PUTIH JANTAN Pangestu, Prayugo Sugeng; Permadi, Yulian Wahyu; Wirasti, Wirasti
Prosiding University Research Colloquium Proceeding of The 8th University Research Colloquium 2018: Bidang MIPA dan Kesehatan
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

Terung ungu (Solanum melongena L.) memiliki kandungan yang dapat memperlancar sirkulasi darah. Penelitian ini bertujuan untuk mengetahui efek afrodisiak ekstrak etanol buah terung ungu terhadap libido tikus putih jantan galur Wistar yang berumur 2-2,5 bulan. Penelitian dibagi 4 kelompok : Kontrol negatif (aquades 1 ml); EET Dosis1 (91,88 mg/130 g BB); EET Dosis2 (183,77 mg/130 g BB), K (+) (viagra) (0,585 mg/130 g BB). Pengamatan dilakukan dengan mengamati aktivitas seksual tikus putih jantan yaitu introducing dan mounting dengan alat bantu CCTV selama 7 hari dari pukul 19.00 WIB – 06.30 WIB. Data dianalisis menggunakan uji ANAVA satu arah, dilanjut dengan uji Tukey HSD dengan ? = 0,05, kemaknaan berdasarkan nilai p<0,05. Hasil pengamatan menunjukkan bahwa ekstrak etanol buah terung ungu memiliki aktivitas afrodisiak terhadap libido tikus putih jantan galur wistar pada dosis 183,77 mg/130 g BB.
Formation and Capasity Building Of Herbal Healthy Cadre in Api-Api Village, Wonokerto, Pekalongan Regarding TOGA Wirasti, Wirasti; Rahmatullah, St.; Permadi, ulian Wahyu
Prosiding University Research Colloquium Proceeding of The 15th University Research Colloquium 2022: Bidang Pengabdian Masyarakat
Publisher : Konsorsium Lembaga Penelitian dan Pengabdian kepada Masyarakat Perguruan Tinggi Muhammadiyah 'Aisyiyah (PTMA) Koordinator Wilayah Jawa Tengah - DIY

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Abstract

VillageApi-apui WonokertoDisctrict,PekalonganRegency locatedapproximately6 km to the north Pantai Utara Jawa (Pantura). The livelihoods of the Api-api villages communityisfisherman,pond farmers,convectionfarmers, rice farmer ect. The health level of the Api-Api community was initially relatively good because awareness for treatment was good, but after the rob and the covid-19 pandemic, the health of the community village declined and the environment became less well -maintained. To support and further improve the level of public health, it necessary to form herbal healthy cadre. The aim of this activity servicecommunityis to increase and empower community in Api-Api Vilage, Wonokerto District, Pekalongan Regency. The Service vommunityiscarriedout inseveralstage. The first stage:observation,thesecondstage is a shared perceptionwith thevillagehead,the thirdstageiscounselingon how to plant family medicinalplants(TOGA),the fourth stage istrainingon how to select and growmedicinal plants. ThisMethodsactivity iscollaborationwith the villagegovermantand training. The results of this activity is an increase inknowledgeandundrstandingof herba; healthy cadres in Api-Api Village by 38,2%. The community (Herbal healthy cadres) incerased their knowledge about how select and grow medicinal plant . TheConclusionthis activityis to increase capacityofknowledgeplantmedicinaland how toselectas well as plantTOGA.
Analisis Kadar Seng (Zn) dan Besi (Fe) Pada Kangkung Air (Ipomoea aquatica Forssk.) Berdasarkan Tempat Tumbuh Dengan Metode AAS (Atomic Absorption Spectrophotometry) Khoirunnisa, Aishwarya; Nur, Achmad Vandian; Rahmasari, Khusna Santika; WIrasti, Wirasti
Bioma : Berkala Ilmiah Biologi Vol. 25 No 2, Tahun 2023
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.2023.57317

Abstract

Water spinach (Ipomoea aquatica Forssk.) is a vegetable that is widely found and consumed by the public, containing among others protein, calcium, phosphorus, iron, potassium, zinc and vitamins A, B1 and C. Zn is a micronutrient that functions for growth and development of bone metabolism. Fe is a micromineral for the formation of hemoglobin in the blood. The purpose of this study was to determine the content and amount of zinc and iron in water spinach based on different growing places using the AAS (Atomic Absorption Spectrophotometry) method. The sample used in this study was water spinach (Ipomoea aquatica Forssk.) taken from 3 locations, namely Sapugarut, Coprayan and Kandang Panjang. The method in this study was qualitatively using color testing and quantitatively using AAS. The results of qualitative testing on Fe produced a brownish color and Zn produced a clear solution with a white precipitate. Quantitative test results using the AAS method at a wavelength of 248.3 nm for zinc and 213.9 nm for iron. The Zn content in the sample obtained was sample A of 0.3869 mg/100 g, sample B of 0.5199 mg/100 g and sample C of 0.2556 mg/100 g while the level of Fe in sample A was 2.502 mg/100 g, sample B was 2.275 mg/100 g and sample C was 1.925 mg/100 g. The results of the study showed that the Zn and Fe levels had different levels, this was because the nutrient content in the soil had different nutrients.
Uji Aktivitas Antiinflamasi Fraksi Metanol dan Fraksi n-Heksan Daun Asam Jawa (Tamarindus indica) dengan Metode Stabilisasi Membran Sel Darah Merah secara Invitro Anggara, Aulia Fajjar; Wirasti, Wirasti; Waznah, Urwatul
Sinteza Vol. 1 No. 1 (2021): February
Publisher : Universitas Hamzanwadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (123.759 KB) | DOI: 10.29408/sinteza.v1i1.3204

Abstract

Buah asam jawa digunakan masyarakat sebagai antiinflamasi atau peradangan. Tanaman asam jawa (Tamarindus Indica) menghasilkan senyawa-senyawa organik seperti senyawa alkaloid, flavonoid, fenolik, tanin dan saponin. Antiinflamasi merupakan obat yang bekerja dengan cara menghambat hormon pemicu peradangan. Tujuan penelitian ini yaitu mengetahui aktivitas antiinflamasi fraksi methanol dan n-heksan daun asam jawa (Tamarindus indica) pada sampel darah sapi. Metode penelitian ini yaitu eksperimental menggunakan metode HRBC (Human Red Blood Cell) secara invitro. Data yang diuji adalah besarnya absorbansi pada masing-masing sampel, analisis data menggunakan ANOVA satu arah. Dilanjutkan dengan uji tukey untuk melihat perbedaan antar kelompok. Hasil dari penelitian ini adalah ekstrak daun asam jawa, fraksi metanol dan fraksi n-heksan dapat menstabilkan membran sel darah merah. Partisi metanol dan partisi n-heksan dengan konsentrasi 1000 ppm mempunyai efek stabilisasi membran. Fraksi metanol memiliki daya stabilisasi membran 89,9% lebih besar dibandingkan fraksi n-heksan 86,6% dan senyawa yang diperkirakan mempengaruhi stabilisasi membran yaitu, alkaloid, flavonoid, tannin, saponin dan steroid. Simpulan ekstrak daun asam jawa, fraksi metanol dan fraksi n-heksan efektif untuk antiinflamasi dengan metode stabilisasi membran sel darah merah. Saran perlu dilakukan penelitian lebih lanjut terhadap senyawa yang berpengaruh pada aktivitas antiinflamasi stabilisasi membran sel darah merah dengan metode yang lain.
UJI AKTIVITAS ANTIOKSIDAN DAN PENGHAMBATAN ENZIM KOLAGENASE EKSTRAK ETANOL UWI UNGU (Discorea alata L): Uji Aktivitas Antioksidan dan Penghambatan Enzim Kolagenase Ekstrak Etanol Uwi Ungu (Discorea Alata L) Izzudin, Muhammad Izzudin Lutfi; Wirasti, Wirasti; Waznah, Urmatul; Nur, Achmad Vandian
Jurnal Ilmiah JOPHUS : Journal Of Pharmacy UMUS Vol. 6 No. 2 (2025): Februari
Publisher : Program Studi Farmasi, Universitas Muhadi Setiabudi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46772/jophus.v6i2.824

Abstract

Antioksidan merupakan senyawa yang berperan dalam menangkal radikal bebas. Radikal bebas merupakan senyawa yang bersifat reaktif dan tidak stabil selain itu memiliki sifat yang merusak sel dan jaringan. Antioksidan yang berasal dari bahan alam teruji secara klinis efektif dalam menangkal radikal bebas. Uwi ungu (Dioscorea alata L.) merupakan umbi yang memiliki kandungan tinggi antioksidan karena adanya senyawa flavonoid. Antioksidan ini dapat digunakan dalam penghambatan enzim kolagenase dalam memicu terbentuknya kerutan pada kulit. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dan penghambatan enzim kolagenase pada ekstrak etanol uwi ungu. Uwi ungu diesktraksi secara maserasi lalu dilakukan pengujian antioksidan dengan metode DPPH (2,2-Diphenyl-1-picrylhydrazyl) dan penghambatan enzim kolagenase menggunakan FALGPA sebagai substrat dan 1,10-Phenanthroline sebagai kontrol positif dan Buffer kolagen sebagai control inhibitor. Aktivitas antioksidan dan penghambatan enzim kolagenase dinyatakan dalam (Inhibiton Concentration 50) IC50. Hasil penelitian menunjukkan bahwa ekstrak etanol uwi ungu memiliki IC50 yaitu sebesar 64,59 µg/mL dan juga memiliki aktivitas penghambatan enzim kolagenase sebesar 23,80 %. Data yang diperoleh dianalisis statistik menggunakan uji Analysis of Variance (ANOVA) untuk mengetahui perbedaan antar kelompok perlakuan dan kelompok perlakuan yang terbaik.Kata kunci : Ekstrak uwi ungu, antioksidan, DPPH, enzim kolagenase, IC50
Stabilitas Membran Sel Eritrosit Ekstrak, Fraksi Kloroform, Etil Asetat dan Metanol Herba Patah Tulang (Euphorbia Tirucalli L.) Fika, Irma; Wirasti, Wirasti; Pambudi, Dwi Bagus; Waznah, Urmatul
JOURNAL OF PHARMACY SCIENCE AND TECHNOLOGY Volume 5 No. 1: Juli 2024
Publisher : Prodi Farmasi Fakultas Kedokteran Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/pst.v5i1.402

Abstract

Euphorbia tirucalli L. (patah tulang) contains secondary metabolites with potential anti-inflammatory activity. This study aimed to identify its bioactive compounds and evaluate erythrocyte membrane stabilization of the extract and fractions. Extraction was performed by maceration with 96% ethanol, followed by fractionation using chloroform, ethyl acetate, and methanol. Total flavonoid and phenolic contents were measured by UV-Vis spectrophotometry, while erythrocyte membrane stabilization was tested in vitro. Phytochemical screening revealed flavonoids, phenols, saponins, steroids, triterpenoids, and tannins. The phenolic content was 432.1 ± 99.4 mg/g in the extract, 1,057 ± 88.02 mg/g in the chloroform fraction, 254.4 ± 48.84 mg/g in the ethyl acetate fraction, and 643.2 ± 267.3 mg/g in the methanol fraction. The highest flavonoid content was found in the chloroform fraction (4.26 ± 0.12 mg/g), followed by the extract (3.63 ± 0.35 mg/g), ethyl acetate (3.56 ± 0.36 mg/g), and methanol (2.6 ± 0.03 mg/g). Membrane stabilization assays showed the extract provided the strongest protection (95.04%), followed by chloroform (89.51%), ethyl acetate (87.71%), and methanol (87.52%), approaching the positive control diclofenac sodium (90.59%). Kruskal-Wallis and Tukey tests indicated the ethanol extract at 100 μg/mL (p=0.997 >0.001) and chloroform fraction at 500 μg/mL (p=0.023 >0.001) were not significantly different from the control, while ethyl acetate and methanol fractions differed significantly (p<0.001). These results demonstrate that the ethanol extract and chloroform fraction possess the highest anti-inflammatory potential, supported by high phenolic and flavonoid levels and consistent statistical analysis.
Co-Authors Achmad Vandian Nur, Achmad Vandian Ainun Muthoharoh Anggara, Aulia Fajjar Anjani, Lestari Dwi Antonius Padua Ratu Arief Nurrochmad Dewi Syarifah Dirman, Arifin Dwi Bagus Prambudi Eko Mugiyanto Eko Mugiyanto, Eko Endang Lukitaningsih Fika, Irma Indriyani, Puspa Irma Suryani Isyti&#039;aroh Isyti&#039;aroh Izzudin, Muhammad Izzudin Lutfi Khoirunnisa, Aishwarya Khusna Santika Rahmasari Khusnul Milatul Khasanah Mahdanya Putri A.B Mufrodah, Nely Nanang Fakhrudin, Nanang Pambudi, Dwi Bagus Pangestu, Prayugo Sugeng Permadi, ulian Wahyu Prayugo Sugeng Pangestu Rahmatullah Rahmatullah Rahmatullah, ST. Rahmawati, Adillah Safitri, Arista Siti Khuzaiyah Slamet . Slamet Slamet St. Rahmatullah St. Rahmatullah Syarifah, Dewi Titi Lestari Umarella, Novel Urmatul Waznah Wahyu Permadi, Yulian Waznah, Urwatul Yulian Wahyu Permadi