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All Journal JPS Unimus Biology, Medicine, & Natural Product Chemistry JRST (Jurnal Riset Sains dan Teknologi) Bioma : Jurnal Biologi Indonesia Abdimas Dewantara Media Ilmu Kesehatan Adimas : Jurnal Pengabdian Kepada Masyarakat Al-Tamimi Kesmas: Jurnal Ilmu Kesehatan Masyarakat (Journal of Public Health Sciences) Al-Asalmiya Nursing: Journal of Nursing Sciences jurnal abdimas saintika Jurnal Kesehatan Saintika Meditory Jurnal Kesehatan Jurnal Amanah Kesehatan Jurnal Ilmu Kesehatan Bhakti Setya Medika Jurnal Ilmu Kedokteran dan Kesehatan Indonesia Jurnal Ilmu Kedokteran dan Kesehatan Indonesia
Rahmatullah, Widia
POLTEKKES BHAKTI SETYA INDONESIA
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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IDENTIFICATION OF BACTERIAL CONTAMINATION ON MEDICAL SCISSORS BY BACTERIAL CULTURE METHOD IN THE AFTAP LABORATORY OF BHAKTI SETYA INDONESIA HEALTH COLTECH rahmatullah, widia; Hermawan, Danar; R, Rudina Azimata
Jurnal Kesehatan Saintika Meditory Vol 8, No 2 (2025): November 2025
Publisher : STIKES Syedza Saintika Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30633/jsm.v8i2.3339

Abstract

The room and equipment used for blood component processing must comply with the quality management system with one of the objectives to eliminate the risk of bacterial contamination in the blood supply unit. Medical scissors in the blood tapping process act as medical tools used to cut the blood bag tube after the blood volume obtained has been fulfilled. This study aims to determine and identify the presence of bacterial contamination in medical scissors in the blood tapping room (AFTAP) Poltekkes Bhakti Setya Indonesia Yogyakarta. This research method is True Experiment with The Posttest Only Design research design, namely the results of observations provide descriptive information. The samples taken were five medical scissors in the Aftap room Poltekkes Bhakti Setya Indonesia. The sampling method used is Quota sampling. The results of the study on the identification of bacterial contamination in medical scissors in the Blood Tapping Laboratory (AFTAP) Poltekkes Bhakti Setya Indonesia Yogyakarta found bacterial growth with details of 20 samples containing Gram Positive bacilli and cocci. The most commonly found bacteria are Gram Positive bacteria with a rod shape (Bacillus). 
Washed Erythrocyte (WE) Bag Bacteria Brevundimonas vesicularis Identification at the Blood Donor Unit PMI DKI Jakarta Widia Rahmatullah; Rina Oktaviyanti; Resmi Aini; Rudina Azimata R.; Reska Handayani
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.693-698

Abstract

A Packed Red Cell (PRC) component known as a washed erythrocyte (WE) has undergone two to three washings with sterile physiological saline to get rid of 99% of the plasma protein, antibodies, and some leukocytes. The goal of this study was to use Bact/alert media to detect the presence or absence of bacteria (anaerobes or aerobes) in WE blood components because there is a chance that germs from the environment could contaminate WE processing because it is still an open system and the manufacturing is still exposed to the elements. Blood samples come from WE blood products that have undergone quality checks for bacterial contamination in a lab dedicated to product quality. Checking for bacterial contamination in the blood involves utilizing the Bact / Alert tool to examine bacterial contamination. Blood product aerobic (BPA) and blood product anaerobic (BPN) media are used in the culture of blood product sample on Bact/alert. Aside from 1 (2.44%) component sample of WE blood that was identified as bacteria in BPA media, the results of the 41 samples examined in 2020 showed that there was aerobic bacterial contamination in BPA media but no anaerobic bacterial contamination in BPN media. This was confirmed by the examination of samples on BPN media, which yielded negative results in 41 samples (100%) and positive results from none of the 41 samples. The results of identification at the Microbiology Laboratory at the University of Indonesia showed that the aerobic bacteria that contaminated washed erythrocyte (WE) blood products on BPA microbiology media were Brevundimonas vesicularis.
Co-Authors Abdullah, Sulistiawati Ana Dewi Lukita Sari Ana Mardiyaningsih Azimata R, Rudina Azimata Rosyidah, Rudina B. Labito, Rahmin burhan, Amelia handayani Dahlia Ayu Pri Irani Fitriana Yulianti Frisilia Gryas Triesyulianti Hamida Ismaqonita Handayani, Reska Handyani, Reska Hendra Rohman Hermawan, Danar Hery Setiyawan Hery Setiyawan Kuswandari, Roselina Muflih, Arifin Munawarah Ningrum, Aulia Tata Pri Irani, Dahlia Ayu R, Rudina Azimata Reska Handayani Resmi Aini Resmi Aini Rina Oktaviyanti Rina Widiastuti Widiastuti Rosyidah, Rudina Azimata Rudina Azimata R. Rudina Azimata Rosyidah rudina azimata rosyidah Sari, Ana Dewi Lukita Shinta Shinta Shinta Shofiana Amini Rizki Sulaiman, Fadia Yonast Berliana Yonast Berliana Zainal Arifin Zona Putra, Resdika