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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati AGRIVITA, Journal of Agricultural Science Makara Journal of Science Filogeni: Jurnal Mahasiswa Biologi
Yusuf, Adib Fakhruddin
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology Veterinary

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Journal : Makara Journal of Science

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Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
Publisher : UI Scholars Hub

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Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Inter-Simple Sequence Repeats Application on the Identification of Genetic Variation of a New Indonesian Melon Cultivar “Melona” Salsabila, Tantri Ajeng Salma; Yusuf, Adib Fakhruddin; Wibowo, Wiko Arif; Kusnanda, Prima Sekti; Daryono, Budi Setiadi
Makara Journal of Science Vol. 29, No. 1
Publisher : UI Scholars Hub

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The field of identification of molecular markers has seen significant advancements, with the Inter-Simple Sequence Repeat (ISSR) emerging as a prominent tool for characterizing genetic variation within species. This study focuses on the new Indonesian melon cultivar “Melona,” developed through selective breeding of commercial melons. This study aimed to assess the utility of ISSR markers in evaluating the genetic variation and stability of the “Melona” cultivar. Comparisons were made with established cultivars—‘Inthanon,” “Rich Sweetness,” “Chamoe,” and “Hikapel”—using five ISSR primers: UBC 807, UBC 808, UBC 810, UBC 812, and UBC 826. A total of 70 DNA fragments with a high polymorphism rate were produced, thereby providing insight into phenetic relationships among the cultivars with simi-larity indices <70%. These genetic distinctions were corroborated through morphological evaluations. These findings indicate that ISSR markers effectively reveal genetic variation within species, offering a practical approach for devel-oping new plant varieties in the Cucurbitaceae family.
Co-Authors Angellya, Bunga Finovel Anjar Tri Wibowo Aurantika, Rindu Awik Puji Dyah Nurhayati BUDI SETIADI DARYONO Ganies Riza Aristya Hasanah, Muslifah Hasibuan, Aldy Riau Wansyah Hidayat, Madyan Akmal Indra Lesmana Karmilah, Karmilah Kusnanda, Prima Sekti Latifah, Vida Rahma Meitha, Karlia Meriem, Selis Miftahudin . Nainggolan, Ananto Puradi Niken Satuti Nur Handayani Nugroho, Giri Nurcahyati, Vivi Indah Nurdilfa, Nurdilfa Nurhasanah, Anggun Diyan Pratiwi, Arini Dian Putro, Karso Suryo Salsabila, Tantri Ajeng Salma Sartika, Dian Setiyobudi, Rizal Hermawan Sulaiman, ‪Teuku Nanda Saifullah TATIK CHIKMAWATI Tuty Arisuryanti, Tuty Wibowo, Wiko Arif Widyasari, Adristi Shafa