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PENGEMBANGAN INOKULUM UNTUK MENINGKATKAN MUTU GIZI TEMPE Mien Karmini; Hermana Hermana; Erwin Affandi
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) JILID 18 (1995)
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v0i0.2230.

Abstract

Inokulum tempe yang lazim digunakan di Indonesia mengandung Rhizopus Sp. Penelitian terdahulu menemukan dua jenis bakteri penghasil vitamin B12, yaitu Citrobacter freundil dan Klebsiella pneumoniae, dan dua jenis bakteri penghasil Faktor-2, yaitu Corynebacterium Sp dan Micrococcus luteus dari air rendaman kedelai. Dalam penelitian ini dibuat inokulum tempe yang merupakan campuran kapang Rhizopus oligosporus dan masing-masing bakteri Klebsiella pneumoniae atau Citrobacter freundii mampu meningkatkan kadar vitamin B12 di dalam tempe lebih dari 100%. Inokulum campuran Rhizopus oligosporus dan Corynebacterium Sp atau Micrococcus luteus tidak menunjukkan adanya kandungan antioksidan Faktor-2 di dalam tempe.
PENGARUH FERMENTASI TERHADAP KANDUNGAN PROTEIN DAN KOMPOSISI ASAM AMINO DALAM SINGKONG Almasyhuri Almasyhuri; Endi Ridwan; Heru Yuniarti; Hermana Hermana
Penelitian Gizi dan Makanan (The Journal of Nutrition and Food Research) JILID 22 (1999)
Publisher : Persagi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/pgm.v0i0.1529.

Abstract

Effects of Solid Fermentation on Protein Content and Amino Acid Composition of Cassava.This study was carried out to assess the protein and amino acid quantity of solid fermentation of cassava (Manihot esculenta) using pure culture of the Rhizopus oligosporus and traditional inoculum (laru). The protein content of the fermented product was analyzed by Biuret method, and the animo acid composition by HPLC (high performance liquid chromatography) method. The results showed that solid fermentation of cassava increased the protein content from 2.1% to 4.0% and 4.7%. The animo acid contents of the fermented product increased 2.5 folds of that of cassava. Higher increase was seen in substrates fermented with traditional inoculum. This is due to the addition of coconut oil and ammonium to the cassava substrate which improved the growth of mold.Key words: cassava (Manihot esculenta), food fermentation, protein content, amino acid composition