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DETEKSI KONTAMINAN FRAGMEN DNA PENGKODE cyt b BABI PADA SAMPEL SOFTGELLCANDY TAK BERLABEL HALAL Yuanita Rachmawati; Saiku Rokhim; Misbakhul Munir; Eva Agustina
Indonesia Journal of Halal Vol 1 (1) 2018
Publisher : Pusat Kajian Halal Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.92 KB) | DOI: 10.14710/halal.v1i1.3115

Abstract

Abstrak Softgellcandy adalah permen bertekstur lunak yang diproses dengan penambahan komponen hidrokoloid seperti agar, gum, pektin, pati, kaegenan, gelatin dan lain-lain yang digunakan untuk modifikasi tekstur sehingga menghasilkan produk yang kenyal. Sayangnya distribusi Softgellcandy di pasaran seringkali terlepas dari pengawasan lembaga berwenang. Banyak ditemukan bermacam merk Softgellcandy yang tidak berBPOM maupun tidak berlabel Halal. Gelatin menjadi titik kritis kehalalan Softgellcandy. Penelitian ini menguji 15 sampel Softgellcandy tak berlabel halal yang dijual bebas di Surabaya dengan primer pengkode fragmen DNA cytochrome b Babi. Metode yang digunakan adalah konvensional PCR pada suhu 98oC-2 menit; 95oC-30 detik; 61oC-30 detik; 72oC-40 detik; dan 72oC-3 menit, selama 30 siklus. Visualisasi hasil PCR menggunakan elektroforesis 2% gel agarosa menunjukkan dari 15 sampel, 8 sampel terindikasi kontaminan DNA babi ditandai dengan pita DNA sebesar ±149bp. Pemerintah perlu melakukan monitoring lebih ketat terkait peredaran produk makanan tak berlabel halal yang dijual bebas di pasaran, mengingat Halal menjadi issue yang sangat sensitif di negara dengan mayoritas penduduk muslim terbesar di dunia ini. Karena halal adalah suatu keharusan. Keywords: Softgellcandy, cyt b Babi, PCR
UJI AKTIVITAS SENYAWA ANTIOKSIDAN DARI EKSTRAK DAUN TIIN (Ficus Carica Linn) DENGAN PELARUT AIR, METANOL DAN CAMPURAN METANOL-AIR EVA AGUSTINA
KLOROFIL: Jurnal Ilmu Biologi dan Terapan Vol 1, No 1 (2017): Klorofil: Jurnal Ilmu Biologi dan Terapan
Publisher : Program Studi Biologi Fakultas Sains dan Teknologi Universitas Islam Negeri Sumatera Utara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30821/kfl:jibt.v1i1.1240

Abstract

Antioxidants can ward off free radicals in the body to resist oxidative damage caused by free radicals. The aim of this research is to know the influence of water solvent, methanol and methanol-water mixture to antioxidant activity. The extraction is done by maceration method. The extraction results in the phytochemical test and functional group analysis to determine the compounds contained in Pig leaf extract. Further testing of antioxidant activity with DPPH method. Pig leaf extract with methanol solvent has antioxidant activity with IC50 3,3005 μg / ml value, Pig leaf extract with water solvent has antioxidant activity 3,6976 μg / ml and Pig leaf extract with methanol solvent: water has antioxidant activity 13,6140 μg / ml. The IC50 <50 μg / ml value indicates that the Pig leaf extract with some solvents has potent antioxidant potential. Pig leaf extract with methanol solvent has the best antioxidant activity with IC50 3,3005 μg / ml value because according to phytochemical test and functional group analysis that methanol solvent is able to extract more active compound such as flavonoid, Triterpenoid and Sterol, Alkaloid and Saponin followed by Pig leaf extract with water solvent and methanol mixture: water.
Respon Tanaman Daun Tombak (Sagittaria lancifolia) Dalam Cekaman Logam Berat Tembaga (Cu) Alfin Fatwa Mei Afifudin; Eva Agustina; Nirmala Fitria Firdhausi; Rony Irawanto
JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Vol 7, No 2 (2022): Mei 2022
Publisher : Universitas Al Azhar Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36722/sst.v7i2.1118

Abstract

One type of heavy metal that pollutes waters is heavy metal copper (Cu). Cu metal is basically an essential metal for living things, but with high levels of Cu metal, it can interfere with the body's metabolic processes. Therefore, there needs to be a solution to overcome Cu metal pollution in the waters. One of them is phytoremediation, or the use of plants to absorb pollutants. Spear leaf plant (Sagittaria lancifolia) is proven to be able to absorb heavy metal copper (Cu). However, the growth response is not yet known in more detail. Therefore, this study aimed to determine the growth response of pike leaf (Sagitaria lancifolia) under stress of heavy metal copper (Cu). This research is an experimental study with a completely randomized design (CRD). The experiment was conducted with 8 treatments (4 treatments with 2 tests) and 3 replications. The 4 treatments were distinguished based on differences in the concentration of Cu metal used, namely 0 mg/L, 1mg/L, 2mg/L, and 3 mg/L. The results showed that exposure to heavy metal copper (Cu) in each concentration variation and detention time variation did not have a significant effect on the growth and morphological conditions of spear leaf (Sagittaria lancifolia) plant, so this plant deserves to be used as an option in recovery efforts of water environment contaminated with heavy metal copper (Cu).Keywords – Response, Lanceleaf arrowhead, Sagittaria lancifolia, Heavy metals, Copper (Cu)
Pengaruh Konsentrasi Gula Terhadap Karakteristik Fisika, Kimia dan Aktivitas Antioksidan Teh Kombucha Daun Belimbing Wuluh (Avverhoa bilimbi Linn.) Fanny Fajrin Aulia Rosyada; Eva Agustina; Hanik Faizah
Rekayasa Vol 16, No 1: April 2023
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v16i1.16977

Abstract

One of the fermented traditional drinks that can have a health effect is kombucha. The materials that can be used in making kombucha is wuluh starfruit leaves. The research was conducted to determine the effect of sugar concentration of the physical, chemical characteristic, and antioxidant activities of wuluh starfruit leaf kombucha tea. This research is an experimental study with a complete randomized design method (CRD) consisting of 3 treatments. Kombucha wuluh starfruit leaves are made with a sugar concentration of 10%, 15% and 20%. The physical characteristics tested include organoleptic tests and the chemical characteristics include a test of the pH value, the total levels of titrated acids (tat), phenolics statistically  analyzed  using  the Kruskal Wallis test and the Mann Whitney test. The alcohol data and antioxidant activity (IC50) were analyzed descriptively. The results of the Kruskal Wallis test  have a significant difference in the pH. The best result based on the physical, chemical characteristic and antioxidant activity is were kombucha with a sugar concentration of 10% with a pH value of 3, a tat content of 0.11%, a phenolic content of 87.33 mg/ml GAE, an alcohol content of 0.41% and an IC50 value of 3.65 ppm.
Assessing the Tolerance of Sagittaria Lancifolia (Spear Plant) to Linear Alkylbenzene Sulfonates (LAS) Exposure Salsa Nabila; Risa Purnamasari; Eva Agustina; Rony Irawanto
Indonesian Journal of Biology Education Vol. 6 No. 1 (2023): INDONESIAN JOURNAL OF BIOLOGY EDUCATION
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

One of pollutant that commonly found in aquatic environment is detergent. Detergent pollution can cause skin irritation and other health problems in humans and animals that use contaminated water sources. To address this issue, it is critical to implement effective policies and regulations to control detergent pollution and promote sustainable wastewater management practices in Indonesia. One of the approaches to overcome detergent pollution is to use plants as pollutant absorbing agents, or commonly called phytoremediation techniques. Therefore, this study aims to determine the growth response of spear plant (Sagittaria lancifolia) in response to the stress of Linear Alkylbenzene Sulfonate (LAS) detergent. This research is an experimental research using a completely randomized design (CRD). There were four treatments tested with three replicates for each treatment. Treatment variations were based on the concentrations used in this study, namely 0 mg/L, 10 mg/L, 30 mg/L, and 50 mg/L. The research was conducted for four months, starting from July to October 2022, and was conducted in the greenhouse of Purwodadi Botanical Garden – BRIN. The result showed that although it experienced some morphological changes such as leaf drying, discoloration, and structural changes, Sagittaria lancifolia were able to survive and conduct metabolic activities properly during 28 days of Linear Alkylbenzene Sulfonates (LAS) pollutant stress.  Thus, Sagittaria lancifolia are able to be used as one of the strategies in restoring the aquatic environment polluted by domestic waste, including LAS detergent.