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All Journal REKAYASA Biology, Medicine, & Natural Product Chemistry BIOMA Health Notions Indonesia Journal of Halal KLOROFIL: Jurnal Ilmu Biologi dan Terapan Pontianak Nutrition Journal (PNJ) JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI KLOROFIL: Jurnal Ilmu Biologi dan Terapan Indonesian Journal of Biology Education BIOTROPIC
AGUSTINA, EVA
Department Of Biology, Faculty Of Sciences And Tecnology, Islamic State University Of Sunan Ampel, Surabaya
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Medicine & Pharmacology Nursing Physics Public Health

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Analisis Kadar Glukosa, Hemoglobin, dan Kolesterol Mencit (Mus Musculus) Setelah Diinduksi Aspartam Esti Tyastirin; Risa Purnamasari; Irul Hidayati; Eva Agustina; Moch. Irfan Hadi; Nova Lusiana; Mei Lina Fitri Kumalasari
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.119-125

Abstract

Aspartame has a lower calorie rating than other sweeteners. for example, cyclamate, lactose, saccharin, fructose and maltosa. Although it has low calorie, aspartame have 60 times sweeter than sugar (sucrose). Induction of aspartame can cause elevated levels of blood glucose and damage to the pancreatic β cells of mice. The purpose of this study was to analyze the dosing of aspartame levels; 13 mg/Kg body weight, 39 mg/Kg and 78 mg/Kg and those effect to the levels of glucose, cholesterol and haemoglobin of female mice (Mus musculus). The methode of research laboratory experiments using a complete randomized design (RAL). This study was using 24 mice with 4 treatment groups. Mice were given aspartame during surgical treatment, mice were made to perform measurement of Hb levels, glucose levels and cholesterol levels of neonatal mice. Data analysis using the Kruskal Wallis because Gaussian data but not homogeneous. The results showed an increase in the levels of glucose and cholesterol levels, but statistically there is no difference in glucose levels (p = 0,087), Hb (p = 0,899) and cholesterol (p = 0,168) in mice (Mus musculus) who were given variations of the dose of aspartame.
Identifikasi Senyawa Aktif dari Ekstrak Daun Jambu Air (Syzygium aqueum) dengan Perbandingan Beberapa Pelarut pada Metode Maserasi Eva Agustina; Funsu Andiarna; Nova Lusiana; Risa Purnamasari; Moch. Irfan Hadi
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.108-118

Abstract

Daun jambu air (Syzygium aqueum) dikenal sebagai salah satu obat tradisional, untuk menyembuhkan beberapa infeksi akibat antigen berupa bakteri. Penelitian ini bertujuan untuk mengetahui kandungan senyawa aktif yang terdapat dalam daun jambu air dengan beberapa pelarut pada proses maserasi. Metode ekstraksi yang digunakan dalam penelitian ini adalah ekstraksi dingin berupa maserasi dengan pemilihan pelarut yakni metanol (CH3OH), etil asetat dan n-heksana. Uji fitokimia yang dilakukan adalah uji saponin, flavonoid, alkaloid dan triterpenoid/steroid. Dari uji yang dilakukan diketahui sampel ekstrak daun jambu air dengan pelarut metanol positif mengandung beberapa senyawa aktif berupa flavonoid, saponin, alkaloid dan triterpenoid. Ekstrak daun jambu air dengan pelarut etil asetat dan n-heksana positif mengandung beberapa senyawa aktif berupa flavonoid, alkaloid dan triterpenoid. Pelarut yang paling bagus untuk mendapatkan banyak senyawa aktif adalah metanol. Kata kunci : Jambu air (Syzygium aqueum), maserasi, senyawa aktif
Dogs (Canis lupus familiaris) as Virus Carrier in Indonesia Moch Irfan Hadi; Muhammad Yusuf Alamudi; Mei Lina Fitri Kumalasari; Sri Hidayati; Tatag Bagus Prakarsa; Eva Agustina; Muhamad Ratodi; Misbakhul Munir; Eko Teguh Pribadi; Hanik Faizah
Biotropic : The Journal of Tropical Biology Vol. 2 No. 2 (2018): Biotropic, Volume 2, Nomor 2, 2018
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2018.2.2.100-107

Abstract

A Virus is an individual that cannot be described as an animal or a plant. If animals and plants contain two nucleic acids (DNA and RNA), on the contrary, the virus only has one of them. These nucleic acids can stimulate a complete virus replication cycle. The virus can only replicate and live on a living host if the host is finally dead, then the virus will move on the cells that are still alive. The virus has genetic material which is a protective protein coat called a capsid. Viruses can infect various varieties of organisms, both eukaryotes (animals, plants, protists, and fungi) and prokaryotes (bacteria and archaea). The Virus infects bacteria known as bacteriophage (phage). The Virus can cause serious diseases for humans such as AIDS, HIV, rabies, etc. Dogs belonging to Canidae family are the sibling of wolves, foxes and raccoon dogs. Among all members of Canidae, dogs have the most closely related to wolves which are the ancestors of dogs. The Canidae family generally has a small elongated body, sharp ear and muzzle, sharp smelling, can run fast and can swim. Dogs are human best friends. While taking care of the dogs, they can be attacked by various diseases. The closeness of the relationship between humans and dogs raises the potential for disease transmission, especially zoonosis and pandemics viruses. Keywords: Canis lupus familiaris, Carier, Influenza, virus
Pengaruh Pemberian Ekstrak Daging Buah Kurma Ajwa (phoenix dactylifera) Terhadap Hitung Jenis Leukosit Embrio dan Induk Mencit (mus musculus) Bunting Eva Agustina; Nova Lusiana; Risa Purnamasari
Biotropic : The Journal of Tropical Biology Vol. 3 No. 2 (2019): Biotropic, Volume 3 Nomor 2, 2019
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2019.3.2.135-145

Abstract

Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daging buah Kurma Ajwa (Phoenix dactylifera) terhadap hitung jenis leukosit embrio dan induk mencit (Mus musculus) bunting. Setiap perlakuan dibagi dalam empat kelompok perlakuan ekstrak. Kelompok I (kelompok kontrol), kelompok II (3 kurma setara 3,12 mg/kg BB mencit), kelompok III (5 kurma setara 5, 2 mg/kg BB mencit), dan kelompok IV (7 kurma setara 7, 28 mg/kg BB mencit). Ekstrak diberikan pada masa kebuntingan 14 sampai 18 hari melalui peroral. Pada hari ke 19 kebuntingan dilakukan pembedahan untuk diambil darahnya pada bagian jantung mencit. Hitung jenis leukosit melalui teknik apusan darah dengan mengamati 100 sel leukosit perbesaran 400 kali pada mikroskop. Hasilnya ekstrak daging buah Kurma Ajwa (P. dactylifera) berpengaruh signifikan terhadap hitung limfosit dan basofil, tetapi tidak berpengaruh terhadap hitung monosit, eosinofil dan neutrofil pada embrio mencit. Sedangkan pada induk mencit berpengaruh terhadap hitung eosinofil dan monosit dan tidak berpengaruh terhadap limfosit, basofil dan neutrofil. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak daging buah Kurma Ajwa (Phoenix dactylifera) terhadap hitung jenis leukosit embrio dan induk mencit (Mus musculus) bunting. Setiap perlakuan dibagi dalam empat kelompok perlakuan ekstrak. Kelompok I (kelompok kontrol), kelompok II (3 kurma setara 3,12 mg/kg BB mencit), kelompok III (5 kurma setara 5, 2 mg/kg BB mencit), dan kelompok IV (7 kurma setara 7, 28 mg/kg BB mencit). Ekstrak diberikan pada masa kebuntingan 14 sampai 18 hari melalui peroral. Pada hari ke 19 kebuntingan dilakukan pembedahan untuk diambil darahnya pada bagian jantung mencit. Hitung jenis leukosit melalui teknik apusan darah dengan mengamati 100 sel leukosit perbesaran 400 kali pada mikroskop. Hasilnya ekstrak daging buah Kurma Ajwa (P. dactylifera) berpengaruh signifikan terhadap hitung limfosit dan basofil, tetapi tidak berpengaruh terhadap hitung monosit, eosinofil dan neutrofil pada embrio mencit. Sedangkan pada induk mencit berpengaruh terhadap hitung eosinofil dan monosit dan tidak berpengaruh terhadap limfosit, basofil dan neutrofil.
Kontaminasi Residu Pestisida Organofosfat di Dalam Tanaman Holtikultura Widia Purnamasari; Moch Irfan Hadi; Eva Agustina
Biotropic : The Journal of Tropical Biology Vol. 4 No. 2 (2020): Biotropic, Volume 4 Nomor 2, 2020
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.2020.4.2.98-110

Abstract

Excessive use of organophosphate pesticides is becoming more common these days. This can affect the level of pesticide residues in farmers' crops. Excessive spraying of pesticides can result in environmental pollution both small and large scale. If excessive exposure to these pesticides can cause damage to the ecosystem. The purpose of this study was to determine the level of organophosphate pesticide residues found in horticulture plants. This research method is a systematic review, by analyzing various studies regarding the contamination of organophosphate pesticide residues in horticultural crops. There are 20 studies from within the country. The results obtained in the form of levels of pesticide residues that are still below the threshold of the BMR (Maximum Residue Limit) of pesticides determined by SNI. Two other studies show that no pesticide residue content was detected. Conclusion: This shows that the sample tested is still safe for consumption. Socialization and education about the doses of pesticide use and how to reduce levels of pesticides in horticultural crops are needed to reduce the risk of exposure to pesticides
Effect of Different Concentration of SCOBY Kombucha as an Anti-acne Against Staphylococcus aureus in The Ear of Mice (Mus musculus) Anggun Putri Ferdyanti; Eva Agustina; Hanik Faizah; Risa Purnamasari
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.1143-1150

Abstract

SCOBY (Symbiotic Culture of Bacteria and Yeast) results from symbiosis between yeast and bacteria during fermentation. SCOBY kombucha gel combined with orange peel extract has antibacterial compounds that can inhibit the growth of S. aureus bacteria. The method in this study was to make variations in the concentration of SCOBY in the gel and inject S. aureus bacteria into the ears of mice. After 2x24 hours, the diameter of the inflammation caused was observed and SCOBY kombucha gel was applied (0%, 2%, 4%, 8%, 10%, and 12%), positive control (Mediclin 1%), and negative control (without gel application). The results showed that the higher the concentration of SCOBY, the faster the healing activity of the gel against S. aureus. SCOBY kombucha gel has the fastest healing activity in inhibiting acne pathogen S. aureus 12% SCOBY gel. Inflammation in the ears of mice treated with 12% SCOBY gel had healed by the 90th hour, while in other groups of mice it had only healed at the last observation hour, namely the 114th hour.
Analisis Aktivitas Antioksidan Daun Sawi menggunakan Metode DPPH dan Uv-Vis Spektrofotometri Aisyah Rian Dini; Ayu Pita Sari; Naila Khurriyatul Friskyah Afsokhah Nabi; Aisha Nurlatifa; Annisa Rajiyyah Aulia; Azhwa Nayla Salsabilla; Ines Puspita Sari; Eva Agustina
Pontianak Nutrition Journal (PNJ) Vol 9, No 1 (2026): Maret 2026
Publisher : Poltekkes Kemenkes Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/pnj.v9i1.2203

Abstract

Daun sawi hijau mengandung senyawa fenolik sebagai antioksidan alami, yang bermanfaat bagi kesehatan maupun di bidang industri pangan. Penelitian ini bertujuan untuk menganalisis aktivitas antioksidan pada ekstrak daun sawi hijau secara kuantitatif menggunakan metode DPPH dan spektrofotometri UV-Vis. Penelitian dimulai dengan mengeringkan daun sawi hijau, kemudian bubuk simplisia daun sawi hijau diekstraksi menggunakan metode maserasi dengan pelarut metanol. Setelah itu, dilakukan uji aktivitas antioksidan pada berbagai konsentrasi, yaitu 10-50 ppm. Pengukuran absorbansi dilakukan pada panjang gelombang maksimum 517 nm untuk menentukan persentase inhibisi radikal DPPH. Hasil ekstraksi menunjukkan rendemen sebesar 3,22% yang tergolong rendah. Sementara itu, uji DPPH menunjukkan bahwa nilai persentase inhibisi pada konsentrasi 10-50 ppm berada pada rentang negatif hingga mendekati nol. Nilai IC₅₀ yang didapatkan menunjukkan hasil yang tinggi, sehingga aktivitas antioksidan pada ekstrak sawi hijau tergolong sangat lemah, namun hasil ini tidak dapat dikatakan valid karena persen inhibisi tidak mencapai 50% dalam rentang konsentrasi yang diuji.
Co-Authors Aisha Nurlatifa Aisyah Rian Dini Alfin Fatwa Mei Afifudin Anggun Putri Ferdyanti Annisa Rajiyyah Aulia Ayu Pita Sari Azhwa Nayla Salsabilla Eko Teguh Pribadi Esti Tyastirin Fanny Fajrin Aulia Rosyada Farida Yulianti Funsu Andiarna Hanik Faizah Ines Puspita Sari Irul Hidayati Mei Lina Fitri Kumalasari Misbakhul Munir Moch. Irfan Hadi Muhamad Ratodi Muhammad Muhibbuddin Abdillah Muhammad Yusuf Alamudi N. R Khoirotun Nazilah Naila Khurriyatul Friskyah Afsokhah Nabi Nirmala Fitria Firdhausi Nova Lusiana Risa Purnamasari Rony Irawanto Saiku Rokhim Salsa Nabila Sri Hidayati Tatag Bagus Prakarsa Vida Nofrianinda Vindi Fristy Kartika Widia Purnamasari Yuanita Rachmawati