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All Journal ACTA VETERINARIA INDONESIANA Jurnal Sain Veteriner Jurnal Medik Veteriner Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Abditani : Jurnal Pengabdian Masyarakat Indonesian Journal of Community Services Veterinary Biomedical and Clinical Journal journal of Basic Medical Veterinary Panrannuangku Jurnal Pengabdian Masyarakat Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Prosiding Seminar Nasional Unimus
Kusumarini, Shelly
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Jurnal Medik Veteriner

 1 
Screening Strongyloides spp. Infection from Wild Rodents Implications for Public Awareness and Attitudes on Zoonotic Diseases in Malang City, Indonesia Kusumarini, Shelly; Danuarta, Muhammad Fernanda; Karami, Farhan; Yesica, Reza; Wisesa, Ida Bagus Gde Rama; Yudhana, Aditya; Nurhidayah, Nanis
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.196-206

Abstract

Strongyloidiasis is a nematode parasite with broader distribution proved to infect humans and animals. Strongyloides ratti common endoparasites infected rodent as the most adaptive population in various environments. We have currently raised concerning the neglected tropical diseases (NTDs) situations in Indonesia. This study aimed to understand the incidence of Strongyloides spp. infecting wild rodents in Malang City during October to December 2021 by stratified random sampling. A total of 50 rats (Rattus norvegicus, Rattus rattus diardii, Mus musculus), 26 male (52%) and 24 female (48%). Following 12 juveniles (24%) and 38 adults (76%). Coprology examinations use floatation and sedimentation methods immediately after the gastrointestinal tract's stool collection (GITs). We also administered a limited survey to get responses from 80 people (housewives, sellers, employee, and students) to obtain risk transmission, public awareness, and attitudes. We were continually analyzed data using the chi-squared and Fisher Exact Test. The microscopic examination of stools was 28% positively detected S. ratti. Our study found an association presented between the age of rats and Strongyloidiasis infections (p<0.05). However, the gender of rats did not have a significant association (p>0.05) to the S. ratti several infections. In addition, most participants did not understand the zoonotic disease and these infections, indicating a low-level knowledge 71 (88%), because better education supports the increase of awareness. However, almost all participants have good practice of hygiene and sanitation toward COVID-19 situations 72 (90%). The further study recommends investigating Strongyloidiasis infections in another species and increasing the education program for housewives and sellers in a traditional marketplace to have better knowledge.
Diagnosis, Hematologic Profile, and Treatment of Cystoisosporiasis in Domestic Dog Fadila, Kurnia Asyafia; Kusumarini, Shelly
Jurnal Medik Veteriner Vol. 7 No. 1 (2024): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss1.2024.205-211

Abstract

Cystoisosporiasis infection is caused by Cystoisospora spp. or Isospora spp. in dogs. Cystoisospora spp. are protozoa ordo Eucoccidiorida. Cystoisosporiasis is considered more risky in puppies. Clinical manifestations of cystoisosporiasis tend to be watery diarrhea to melena in puppies which can also be concurrent with vomiting, and tenesmus, whereas in adult dogs known as symptomatic. A female domestic dog, aged >1 yo was kept indoors and had not been vaccinated or anthelmintic drugs. The dog has changed in the consistency of slightly soft feces with a frequency of defecation 1–2 times a day. Diagnose performed using microscopic fecal examination with the floating method found the presence of Cystoisospora spp. oocysts, followed by the calculation of oocysts per gram (OPG) using a two-chamber McMaster to determine the degree of Cystoisospora spp. infection. Blood examination showed leukocytosis, granulocytosis, and hypochromic anemia. The treatment was administered of 15 mg/kg BW sulfadiazine orally every 12 hours for 7 days, 14 mg/kg BW metronidazole every 12 hours for 7 days, and 300 mg Hemafort® every 24 hours for 7 days. After 7 days of treatment, we reported normal fecal consistency and negative parasitic manifestation. Prevention was done by paying attention to treatment, immediately identifying the presence of oocysts, educating owners to avoid animal distress, and maintaining a good hygiene environment.
Phylogenetic and Genetic Variation Analysis of ITS1 Gene of Trypanosoma lewisi in Wild Rats Using Polymerase Chain Reaction Yesica, Reza; Hardian, Andreas Bandang; Oktanella, Yudit; Kusumarini, Shelly; Prasetyo, Gabriela Jeanne Giacynta
Jurnal Medik Veteriner Vol. 7 No. 2 (2024): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol7.iss2.2024.335-348

Abstract

Murine Trypanosomiasis is a disease caused by the blood protozoan Trypanosoma lewisi in rats, with the transmission process mediated by the flea species Xenopsylla cheopis and Nosopsyllus fasciatus. Cases of trypanosomiasis have been documented due to Trypanosoma lewisi infecting rats and humans in various countries. Diagnosis of T. lewisi is typically conducted using polymerase chain reaction (PCR), which amplifies target DNA using specific primers. One such target gene for detection is the Internal Transcribed Spacer-1 (ITS1). Subsequent sequencing of PCR products enables analysis of genetic variation employing parameters such as nucleotide composition, genetic distance, and phylogenetic analysis with MEGA software. Test results based on percent identity values indicated a 98.51% homology of blood samples with the Chinese strain of T. lewisi (FJ011094.1), demonstrating genetic variation. Phylogram reconstruction revealed that samples 18, 19, and 37 of T. lewisi exhibit very close intraspecies relationships with T. lewisi from NCBI genebank with genetic distance ranging from 0.007 to 0.01. While the closest interspecies relationship was found with T. cruzi (KT305857.1) with a genetic distance of (d = 0.61).
Optimization of Primer Candidate Design for Toxocara cati Identification Using PCR Targeting the COX1 and ND5 Genes in Cats Yesica, Reza; Novita, Ni Kadek; Kusumarini R, Shelly; Sabri, Jasni
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.318-328

Abstract

Toxocara cati is an obligate extracellular parasite within the phylum Nematoda. This species is responsible for toxocariasis, a zoonotic disease. The disease transmission occurs via infective eggs, earthworms, cockroaches, birds, and rodents that contain larvae in their tissues. In cats, infection with this parasite can lead to symptoms such as diarrhea, hypoalbuminemia, anorexia, and a distended abdomen. This research aimed to identify an optimal candidate primer design through in silico analysis using BLAST primers from the National Center for Biotechnology Information (NCBI). This study targeted the mitochondrial genes COX1 and ND5 for the molecular identification of T. cati. Using in silico methods, primer candidates were designed and evaluated based on key parameters, including primer length, melting temperature (Tm), GC content, potential for secondary structure formation, and specificity. Candidate primers were screened using the NCBI primer-BLAST tool and validated through BLAST analysis to ensure sequence specificity. The primer pair that met all the criteria for an optimal candidate primer design comprised the forward primer TCND5F (5′-ACTGCTGGCCTTGTATTGGT-3′) and the reverse primer TCND5R (5′-ACACAGAACGCCTAAACCTCA-3′), both targeting the ND5 gene region.
Epidemiology and Spatial Distribution of Parasitic Infections in Cats in Malang, East Java, Indonesia: Risk Factors and Public Health Implications Yesica, Reza; Pamungkas, Hafiz Bintang; Rahardja, Azzam Dhiya’ulhaq; Putri, Denissa Rachmawati Purnama; Kusumarini R, Shelly
Jurnal Medik Veteriner Vol. 8 No. 2 (2025): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss2.2025.398-413

Abstract

Cats are common companion animals that are susceptible to various parasitic infections, particularly under unsanitary environmental conditions. Apart from transmitting zoonotic infections, infected cats may also suffer from metabolic disorders, malnutrition, anemia, and even death. Fecal, hair, and ectoparasite samples from 170 cats (stray and domestic) were collected from 12 districts in Malang. Statistical tests (Chi-square, OR, and RR) and QGIS-based spatial mapping were employed for data analysis. The findings revealed that 46.4% (79/170) of gastrointestinal endoparasite cases involved Ancylostoma spp. (20.6%), Toxocara cati (19.4%), Toxascaris leonina (7.6%), Strongyloides spp. (2.9%), Dipylidium caninum (4.1%), Cystoisospora felis (5.8%), Cystoisospora rivolta (3.5%), and Toxoplasma gondii-like oocysts (1.1%). Ectoparasite infestations accounted for 52.9% (90/170), with Ctenocephalides felis (50.5%), Sarcoptes scabiei (3.5%), Felicola subrostratus (1.1%), and Rhipicephalus sanguineus (0.5%) being recorded. Risk factor analysis revealed a significant correlation (p < 0.05) between lifestyle and the prevalence rates of cat gastrointestinal endoparasite infections and ectoparasite infestations, while sex and altitude showed no significant correlation (p > 0.05) with either. The findings of the study bear significance for both human and animal health, highlighting the importance of interdisciplinary collaboration among preventive medicine, veterinary science, and public health, and providing a valuable evidence base to support policy development and targeted intervention programs aimed at controlling zoonotic parasites in companion animals, particularly in urban and peri-urban areas.
Co-Authors Abror, Nuzula Ahmad Agnes Tageomi Nakita Anisa Wuri Kartika Annadhifa, Chandra Luki Azmi Tsabitah Billa, Frida Ayu Salsana Chandra Luki A. Chandra Luki Annadhifa Danuarta, Muhammad Fernanda Dian Nugraheni Eka Pramystha Hestianah Fadila, Kurnia Asyafia Fajar Shodiq Permata Fane Vandania Farida Puspita Zuhria Fitroh Rafli Firdausi Frida Ayu Salsana B. Frida Ayu Salsana Billa Hanifa, Filza Hannani Vawzia Hardian, Andreas Bandang Ida Bagus Gde Rama Wisesa Ida Wisesa Irawan, Heri Janumurti, Rizkirana Karami, Farhan Lazulfa, Zaky Indana Lestari, Putri Dwi Novita, Ni Kadek Nurhidayah, Nanis Pamungkas, Hafiz Bintang Paramanandi, Dini Agusti Prasetyo, Gabriela Jeanne Giacynta Putri Dwi Lestari Putri, Angelica Maharani Putri, Denissa Rachmawati Purnama Qosimah, Dahliatul Rahardja, Azzam Dhiya’ulhaq Reza Yesica Reza Yesica Sabri, Jasni Salas Salma Sholekhah Salsana Billa, Frida Ayu Sholekhah, Salas Salma Suryo Kuncorojati Tuska, Habib Syaiful Arif Tutik Juniastuti Vandania, Fane Wisesa, I Bagus Gde Rama Wisesa, Ida Bagus Gde Rama Yesica, Reza Yudhana, Aditya Yudit Oktanella, Yudit Yustia Nur Holizah Yustinasari, Lita Rakhma Zaky Indana Lazulfa Zuhria, Farida Puspita