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All Journal Hemera Zoa Jurnal Sain Veteriner AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Prosiding Seminar Biologi Bioeksperimen: Jurnal Penelitian Biologi Edumatsains BERITA BIOLOGI Jurnal Pendidikan Biologi BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biodjati BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Sciscitatio : Journal for Biological Science Jurnal Kedokteran Meditek Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Vinsa Cantya Prakasita
Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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PENGARUH KONSENTRASI EKSTRAK BIJI DAN KULIT BUAH PINANG (Areca catechu L.) TERHADAP MORTALITAS LARVA NYAMUK Aedes aegypti Anjela Noya; Djoko Rahardjo; Vinsa Cantya Prakasita
EduMatSains : Jurnal Pendidikan, Matematika dan Sains Vol 6 No 2 (2022): Januari
Publisher : Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kristen Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33541/edumatsains.v6i2.3429

Abstract

The purpose of this study is to determine the potential extract of areca nut (Areca catechu L.) seeds, skin and the combination of the two extracts in killing the Aedes aegypti larvae. This study used a Completely Randomized Design in the seed extract test with a concentration of 16000, 17000, 18000, 19000 and 20000 ppm and skin extract with concentrations of 7000, 8000, 9000, 10000 and 11000 ppm. The factorial design was used in the combined extract of 0:100; 25:75; 50:50; 75:25 and 100:0. Each treatment used 4 replications. Data were analyzed by ANOVA and then continued by Tukey, LSD and Duncan tests. The last, data were analyzed by probit analysis to determine LC50 and LC90. The result indicates that areca nut seed and skin contain flavonoid, alkaloid, palmitic acid, phenols, terpenoids and steroids which are toxic. The killing power of seed extract was 64-97% (24hours) and 100% in all treatments (48hours). Skin extract was 52-88% (24hours) and 58-93% (48hours), while the combination extract was 55 -93% (24hours) and 95-100% (48hours). Concentration of 19000 ppm of seed extract with 95% mortality at 24 hours was effective when compared to the WHO standard (2005), while the skin extract had a lower LC value than the other two extracts.
Effectiveness of Frangipani Leaf Extract (Plumeria alba Linn.) as a UV Filter Irein Kusuma Angreini; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.3951

Abstract

UV filters are necessary since UV rays are harmful to human skin. UV filter compounds in commercial sunscreen products can cause adverse effects. Thus, natural UV filters containing flavonoids, e.g. Frangipani (Plumeria alba Linn.) leaves, are needed. This research examined the use of flavonoids from P. alba leaf as a UV filter with in vitro and in vivo. Maceration was performed with 70% ethyl alco-hol and liquid-liquid extraction for flavonoid purification. Parameters tested were total flavonoid compounds, SPF value, and clinical symptoms of the melanogenesis inhibition scoring. The purification result was 43.6%. The flavonoid concentration in pure extract (64.4 mg QE /g) was higher than crude extract (57.6 mg QE/g). The highest SPF value (33.88) was observed in pure extract at 1400 mg/mL, while the same concentration of crude extract resulted in a lower SPF value (33.06). Pearson correlation analysis (0.536) indicated a di-rectly proportional relationship between SPF value and extract concentration. In vivo analysis was performed by scoring between 0-4 following the severity of erythema. After 21 days of sunlight exposure, the worst erythema was found in the negative control group (score 1.292). The best result was found in the group treated with pure extract 1400 mg/ml (score 0.542).
Aktivitas Antibakteri Ekstrak Kulit Batang Belimbing Wuluh Terhadap Escherichia coli Penyebab Diare Jade Septhimoranie; Dwi Aditiyarini; Vinsa Cantya Prakasita
Bioeksperimen: Jurnal Penelitian Biologi Vol 8, No 2: September 2022
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v8i2.15749

Abstract

Diare merupakan salah satu gejala infeksi pada saluran cerna, yang dapat disebabkan oleh adanya organisme (bakteri, virus, dan/atau parasit), sehingga menyebabkan feses menjadi lebih cair dan frekuensinya lebih sering dari biasanya. Escherichia coli memiliki presentase yang tinggi dibandingkan bakteri lain pada feses penderita diare. Pengobatan yang dilakukan biasanya dengan menggunakan antibiotik, namun sekarang ini banyak ditemukan antibiotik yang telah resisten. Tanaman obat-obatan dapat digunakan sebagai alternatif dalam mengatasi diare. Salah satunya ialah kulit batang Belimbing Wuluh. Adanya kandungan senyawa yang bersifat antibakteri pada kulit batang Belimbing Wuluh dimungkinkan dapat menghambat pertumbuhan E. coli penyebab diare. Tujuan penelitian adalah untuk mengetahui aktivitas antibakteri ekstrak kulit batang Belimbing Wuluh terhadap E. coli penyebab diare. Pada penelitian ini menggunakan metode ekstraksi dekoktasi. Uji fitokimia dilakukan terhadap flavonoid, alkaloid, dan saponin. Uji total fenol dilakukan dengan menggunakan metode Folin-Ciocalteu. Uji antibakteri dilakukan dengan menggunakan disk diffusion method Kirby-Bauer, dilanjutkan uji MIC dengan metode mikrodilusi serta uji MBC. Analisis data dengan One-Way ANOVA dan post-hoc Duncan. Kulit batang Belimbing Wuluh mengandung senyawa saponin, alkaloid, flavonoid, dan fenol. Hasil rata-rata dari nilai total fenol adalah 25,12 ± 7,73(mg GAE)⁄g. Hasil uji aktivitas antibakteri menunjukkan bahwa ekstrak kulit batang Belimbing Wuluh dengan konsentrasi 2,5% masih dapat menghambat E. coli secara signifikan (P≤0,05).
THE EFFECT OF KESAMBI BARK EXTRACT ON MICE LYMPHOCYTE COUNT AND SPLEEN INDEX Christine Stefani Tamelan; Kukuh Madyaningrana; Vinsa Cantya Prakasita
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 8 No. 2 (2022): February 2022
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v8i2.6039

Abstract

Kesambi is a plant widely used by people of Timor Island. Flavonoid content of Kesambi is potential to be used as immunomodulators. This study aimed  to study the effect of Kesambi bark extract on lymphocyte count and spleen index of male mice as an indicator of their immune responses. Research included identification of phytochemical compounds macerated from Kesambi bark, lymphocytes count and spleen index calculation. Mice injected with sheep erythrocytes were treated orally with three doses of Kesambi extract (0.113 mg/g BW, 0.491 mg/g BW, and 0.712 mg/g BW) for 10 days. Lymphocyte count using blood smear method was carried out on day 0, 5 and 10, while lymphocyte count using hematology analyzer and spleen index measurement were performed after mice sacrificed. Kesambi bark contains alkaloids, flavonoids, tannins, and steroids. Treatment with Kesambi bark extract reduced mice lymphocyte count. Administration of 0.491 mg/gr BW dose reduced the mice lymphocytes count close to the number of lymphocytes in the negative control group. The spleen index of mice had no correlation with changes in the number of lymphocytes. This preliminary study shows a potential use of Kesambi bark extract as a natural immunosuppressant.
Combination of Wild ginger, Honey, and Probiotics as Growth Prometer Candidates : An in Vitro Study Marlin Cindy Claudya Malelak; Agnesia Endang Tri Hastuti Wahyuni; Agustina Dwi Wijayanti; Vinsa Cantya Prakasita
Jurnal Sain Veteriner Vol 40, No 3 (2022): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53256

Abstract

Growth Promoter Antibiotics are used to prevent disease and promote growth and production in poultry. Repeated admnistration of feed can have a microorganic resistance effect, accumulation of antibiotic residues in animal and environmental products and imbalance of normal microflora in the intestine. The antibacterial and carbohydrate content of some natural ingredients can be potential as a replacement candidate for AGP. This study aims to determine the role of a combination of wild ginger, honey, and probiotics (Bacillus subtilis and Lactobacillus acidophilus) as AGP candiate in Vitro. The antibacterial activity of the combination of wild ginger and honey against pathogens (E. coli) and their use against probiotics was tested by disk diffusion method, while the calculation of optical density values to determine the minimum inhibitory concentration and minimum bactericidal concentration was carried out on E. coli. The ability of inhibition of probiotics against pathogens is also done by the disk diffusion method. The disk diffusion test results showed the best combination was extract of 25% wild ginger aquades + 100% Lombok honey with inhibition zone diameter (8,53 ±, 03). Optical density values indicate this combination is able to inhibit and kill E. coli (DO 0,00 ±, 002) and support B. subtilis (DO 0,18 ±, 002) and L. acidophilus (DO 0.25 ±, 005) significantly better than positive control. MIC value of wild ginger aquades extract and honey combination against E. coli is wild ginger aquades extract 3.13% + Lombok honey 25%, while MBC value is wild ginger aquades extract 6,25% + Lombok honey 25%. The combination of B. subtilis and L. acidophilus showed the largest inhibitory zone diameter against E. coli (7,30 ±, 02 mm) compared to individual colonies. The combination of wild ginger and honey, in addition to inhibiting also able to kill pathogens and support the growth of probiotics, so this formula can be used as one of the replacement candidates for AGP.  
Aktivitas Antioksidan dan Antibakteri Ekstrak Etanol Adas Pagar (Eupatorium Capillifolium (Lam.) Small) terhadap Staphylococcus Aureus dan Escherichia Coli Feblicya Palimbunga; Dwi Aditiyarini; Vinsa Cantya Prakasita
BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains Vol 6 No 1 (2023): BIOEDUSAINS:Jurnal Pendidikan Biologi dan Sains
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/bioedusains.v6i1.5361

Abstract

The purpose of the study was to investigate the presence of second-order metabolites and the antibacterial and antifungal activities against S. aureus and E. coli in an experimental manner. This study is being conducted using an experimental method. Metabolit is quickly identified by scrining fitokimia. Antioxidant activity was measured using the DPPH method. Antibacterial activity is carried out using the cakram diffusion method. Ekstraksi was carried out by the 96% etanol-maseration process. The results show that the extracted etanol adas pagar contains flavonoid, alkaloid, tanin, saponin, and terpenoid content. Ekstrak etanol adas pagar has strong anti-oxidant activity with an IC50 of 80,56 ppm. Due to its antibacterial potential, ekstrak etanol adas pagar can kill gram-positive Staphylococcus aureus as well as gram-negative Escherichia coli bacteria when applied in concentrations of 100% and 50%, respectively. The main finding of this study is that ekstrak etanol adas pagar can be used as a safe antibacterial and antifungal against the growth of S. aureus and E. coli. Keywords: Adas Pagar, Antibacterial, Antioxidant, Extraction, Metabolite
Potensi Ekstrak Kulit Buah Pepaya (Carica papaya L.) Untuk Isolasi Kolagen Cumi-cumi (Loligo sp.) Sebagai Penyembuhan Luka Kulit Mencit (Mus musculus) Angelia Astria; Aniek Prasetyaningsih; Vinsa Cantya Prakasita
Al-Kauniyah: Jurnal Biologi Vol 16, No 2 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i2.17629

Abstract

AbstrakKulit buah pepaya merupakan bagian dari buah pepaya yang tidak dikonsumsi dan mengandung enzim papain yang dapat digunakan sebagai pengganti papain murni untuk mengekstraksi kolagen. Tentakel cumi-cumi merupakan salah satu dari bahan baku marine collagen. Ekstraksi kolagen dari tentakel cumi-cumi menggunakan metode maserasi menggunakan CH3COOH dan penambahan ekstrak kulit buah pepaya pada konsentrasi 5, 10, 15, dan 20%. Hasil menunjukkan bahwa konsentrasi 15% ekstrak kulit buah pepaya memberikan rendemen yang terbaik yaitu 19,2% (w). Kolagen yang dihasilkan memenuhi kriteria Badan Standardisasi Nasional (BSN) (2014) pada jumlah mikrobia dan total coliform tetapi belum memenuhi pada kadar protein, pH, dan kadar air. Pada uji preklinis digunakan kolagen hasil ekstraksi dengan penambahan 20% ekstrak kulit buah pepaya yang memenuhi kriteria BSN dengan kadar protein terbanyak. Serum kolagen dibuat menjadi tiga konsentrasi, yaitu 5, 10, dan 20 mg/mL. Hasil pengujian preklinis menunjukkan bahwa kelompok perlakuan kolagen 20 mg/mL memiliki presentase penutupan luka kulit mencit terbaik sebesar 84,61%, dari hasil pengujian statistik menunjukkan tidak ada perbedaan signifikan dengan kelompok perlakuan kontrol positif.AbstractThe papaya peel, which is a part of papaya not typically consumed, contains the papain enzyme that can be used as a substitute for pure papain to extract the collagen. Squid tentacles are one of the mainsources of marine collagen. The extraction of collagen from squid tentacles was conducted using a maceration method with CH3CHOOH solvent, and the papaya peel extract was added at concentrations of 5, 10, 15, and 20%, respectively. The extraction process showed that the addition of 15% papaya peel extract provided the best result of 19.2% (bb). The collagen produced in this experiment met the BSN criteria for total microbes and total coliforms. However, it did not yet comply with the BSN criteria for protein content, pH, and water levels. The preclinical testing utilized the extracted collagen and the papaya peel extract with a 20% concentration, which met the BSN standards with the highest protein level. The serum collagen was divided into three concentrations: 5 mg/ml, 10 mg/ml, and 20 mg/ml. Preclinical studies showed that the treatment group receiving 20 mg/mL collagen exhibited the highest percentage of skin wound healing at 84.61%, and the statistical test found no significant difference compared to the positive control treatment group.
Pengaruh Asap Cair Bambu Tali (Gigantochloa apus) terhadap Pertumbuhan Staphylococcus aureus dan Staphylococcus epidermidis Dessy Imelda Nirmasari Siregar; Dhira Satwika; Vinsa Cantya Prakasita
Jurnal Kedokteran Meditek Vol 28 No 2 (2022): MEI-AGUSTUS
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v28i2.2419

Abstract

Staphylococcus aureus dan Staphylococcus epidermidis merupakan penyebab infeksi pada luka terbuka. Penggunaan antibiotik pada dosis yang berlebih untuk pengobatan infeksi dapat menyebabkan resistensi, oleh karena itu dibutuhkan agen antibakteri alternatif, misal dari tanaman yang dapat membunuh bakteri patogen. Bambu tali merupakan salah satu kekayaan alam yang tumbuh subur di Aek Nauli, Sumatera Utara. Masyarakat setempat menggunakan asap cair bambu tali yang dipercaya dapat mengobati infeksi luka terbuka. Belum banyak kajian potensi asap cair bambu tali, sehingga penelitian ini dilakukan untuk mengkaji potensi asap cair bambu tali sebagai agen antibakteri. Pembuatan asap cair dilakukan dengan melakukan pembakaran bambu tali. Uji fitokimia dilakukan dengan menggunakan GC-MS. Dilakukan uji antibakteri dengan metode difusi agar sumuran, pengukuran nilai MIC dan MBC untuk mengetahui efektivitasnya sebagai agen antibakteri. Hasil GC-MS menunjukkan bahwa senyawa utama yang terkandung dalam cairan asap bambu tali adalah furan, fenol dan asam lemak. Hasil uji antibakteri metode difusi agar menunjukkan bahwa asap cair bambu tali dapat menghambat pertumbuhan bakteri pada konsentrasi 20%. Didapatkan nilai MIC dan MBC 5% asap cair tali bambu terhadap Staphylococcus aureus dan Staphylococcus epidermidis.
Food Composition and Preference of Captive Javan Langur (Trachypithecus auratus) in Gembira Loka Zoo, Yogyakarta Eunnike Adelina; Laurentia Henrieta Permita Sari Purba; Vinsa Cantya Prakasita
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 9, No 3 (2023): Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4860

Abstract

Javan langur (Trachypithecus auratus) is one of the endemic primate species of Indonesia. Its populations were exposed to extinction risk caused by human activities. Thus, conservation efforts such as ex-situ conservation were needed to preserve this species. One of the javan langur ex-situ conservation in Yogyakarta is the Gembira Loka Zoo. In captivity, animals must be guaranteed to be free from hunger and stay nourished. One aspect to support this welfare is food supply. Therefore, this study aimed to identify the food composition and analyze the correlation between food preference and feeding duration of each food item group. We used scan animal sampling method with five minutes intervals to observe the behavior of seven individuals of captive javan langurs. The result showed that there are three groups of food (fruit, vegetable, and leaf) provided daily to javan langurs in Gembira Loka Zoo. All the individuals spent most their time to consumed food from vegetable group, except for infant female who had the highest duration in the leaf group. In addition, based on which food they consumed first every feeding time (preference food), javan langurs preferred vegetable over fruit and leaf. Based on the Spearman correlation test, there are positive correlations of duration and preference of feeding except for leaf groups. This result was different from the natural food composition of javan langurs which mostly consisted of leaves. This study might help the zoo to consider the food items provided for the animals to increase animal welfare.
Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Dwi Aditiyarini; Nita Sonia Sipayung; Vinsa Cantya Prakasita
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

Abstract

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively
Co-Authors Adrenalin, Sruti Listra Agnesia Endang Tri H. Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Agustina Viktoria Tae Ajiguna Ajiguna Anak Agung Gede Sugianthara Angelia Astria Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Anjela Noya Centaury Harjani, Centaury CHARIS AMARANTINI Charles Rangga Tabbu Christine Stefani Tamelan Dessy Imelda Nirmasari Siregar Dhira Satwika Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Enjelin Anjung Susilowati Eunnike Adelina Fauziah, Ima Feblicya Palimbunga Ilham, Jessika Ima Fauziah Ima Fauziah Ima Fauziah Imanjati, Lynda Nugrahaning Inggrid, Annabelle Marie Irein Kusuma Angreini Jade Septhimoranie Jeffi Chandra Kukuh Madyaningrana Laurentia Henrieta Permita Sari Purba Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Maria Trivonia Sema Marlin Cindy Claudya Malelak Nadya Aprina Theodora Nita Sonia Sipayung Pepiana, Marlen Aviati Sarah Prasetya, Heribertus Sigit Priskila Melinia Wuni Relisantika Yulia Sinaga Sema, Maria Trivonia Septhimoranie, Jade Setiawan, Dwi Cahyo Budi Setya Harianto Sidna Artanto Sipayung, Nita Sonia Sitarina Widyarini Sitarina Widyarini Sruti Listra Adrenalin Sruti Listra Adrenalin Sruti Listra Adrenalin Susilowati, Enjelin Anjung Tara Inastu Kandarp Tati Aryani Theodora, Nadya Aprina Thomas Emanuel Manggotu Nahak Yovita Devina Yovita Devina