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All Journal Hemera Zoa Jurnal Sain Veteriner METAMORFOSA Journal of Biological Sciences Jurnal Veteriner AL KAUNIYAH Biosfer: Jurnal Tadris Biologi Prosiding Seminar Biologi Bioeksperimen: Jurnal Penelitian Biologi Edumatsains BERITA BIOLOGI Jurnal Pendidikan Biologi BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biodjati BIOEDUSAINS: Jurnal Pendidikan Biologi dan Sains Jurnal Pembelajaran dan Biologi Nukleus Sciscitatio : Journal for Biological Science Jurnal Kedokteran Meditek
Vinsa Cantya Prakasita
Universitas Kristen Duta Wacana
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Physics Public Health Social Sciences Veterinary Other

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Pengaruh Asap Cair Bambu Tali (Gigantochloa apus) terhadap Pertumbuhan Staphylococcus aureus dan Staphylococcus epidermidis Dessy Imelda Nirmasari Siregar; Dhira Satwika; Vinsa Cantya Prakasita
Jurnal Kedokteran Meditek Vol 28 No 2 (2022): MEI-AGUSTUS
Publisher : Fakultas Kedokteran Universitas Kristen Krida Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36452/jkdoktmeditek.v28i2.2419

Abstract

Staphylococcus aureus dan Staphylococcus epidermidis merupakan penyebab infeksi pada luka terbuka. Penggunaan antibiotik pada dosis yang berlebih untuk pengobatan infeksi dapat menyebabkan resistensi, oleh karena itu dibutuhkan agen antibakteri alternatif, misal dari tanaman yang dapat membunuh bakteri patogen. Bambu tali merupakan salah satu kekayaan alam yang tumbuh subur di Aek Nauli, Sumatera Utara. Masyarakat setempat menggunakan asap cair bambu tali yang dipercaya dapat mengobati infeksi luka terbuka. Belum banyak kajian potensi asap cair bambu tali, sehingga penelitian ini dilakukan untuk mengkaji potensi asap cair bambu tali sebagai agen antibakteri. Pembuatan asap cair dilakukan dengan melakukan pembakaran bambu tali. Uji fitokimia dilakukan dengan menggunakan GC-MS. Dilakukan uji antibakteri dengan metode difusi agar sumuran, pengukuran nilai MIC dan MBC untuk mengetahui efektivitasnya sebagai agen antibakteri. Hasil GC-MS menunjukkan bahwa senyawa utama yang terkandung dalam cairan asap bambu tali adalah furan, fenol dan asam lemak. Hasil uji antibakteri metode difusi agar menunjukkan bahwa asap cair bambu tali dapat menghambat pertumbuhan bakteri pada konsentrasi 20%. Didapatkan nilai MIC dan MBC 5% asap cair tali bambu terhadap Staphylococcus aureus dan Staphylococcus epidermidis.
Food Composition and Preference of Captive Javan Langur (Trachypithecus auratus) in Gembira Loka Zoo, Yogyakarta Eunnike Adelina; Laurentia Henrieta Permita Sari Purba; Vinsa Cantya Prakasita
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS (JPBN) Vol 9, No 3 (2023): Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4860

Abstract

Javan langur (Trachypithecus auratus) is one of the endemic primate species of Indonesia. Its populations were exposed to extinction risk caused by human activities. Thus, conservation efforts such as ex-situ conservation were needed to preserve this species. One of the javan langur ex-situ conservation in Yogyakarta is the Gembira Loka Zoo. In captivity, animals must be guaranteed to be free from hunger and stay nourished. One aspect to support this welfare is food supply. Therefore, this study aimed to identify the food composition and analyze the correlation between food preference and feeding duration of each food item group. We used scan animal sampling method with five minutes intervals to observe the behavior of seven individuals of captive javan langurs. The result showed that there are three groups of food (fruit, vegetable, and leaf) provided daily to javan langurs in Gembira Loka Zoo. All the individuals spent most their time to consumed food from vegetable group, except for infant female who had the highest duration in the leaf group. In addition, based on which food they consumed first every feeding time (preference food), javan langurs preferred vegetable over fruit and leaf. Based on the Spearman correlation test, there are positive correlations of duration and preference of feeding except for leaf groups. This result was different from the natural food composition of javan langurs which mostly consisted of leaves. This study might help the zoo to consider the food items provided for the animals to increase animal welfare.
Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Dwi Aditiyarini; Nita Sonia Sipayung; Vinsa Cantya Prakasita
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

Abstract

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively
Antidiabetic Potential of Ethanol Extract from Moringa oleifera Leaves in Streptozotocin-Nicotinamide-Induced Female Mus musculus Vinsa Cantya Prakasita; Nadya Aprina Theodora; Enjelin Anjung Susilowati; Dwi Aditiyarini; Aniek Prasetyaningsih
Jurnal Biodjati Vol 9, No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30699

Abstract

Type 2 diabetes mellitus (DM) is a chronic metabolic dis­order characterized by elevated blood glucose levels due to insulin resistance. Type 2 diabetes is considerably more prevalent than oth­er forms (85–90%). The risk of type 2 diabetes is higher in women (53.2%). There is an urgent requirement for better and more afforda­ble treatment options considering DM therapy is expensive and may have adverse health effects. The study's objective is to examine how acute toxicity, blood glucose levels, and body weight are affected by Moringa leaf ethanol extract (MLEE). Maceration was employed to eliminate the leaves of the moringa plant. Phytochemical screening was completed to assess the total flavonoid content and screen for alkaloids, flavonoids, phenolics, saponins, and tannins. Acute toxicity testing was performed following OECD guideline 423. Clinical symp­toms of acute toxicity were observed every 30 minutes for the first 24 hours post-treatment, followed by observations every 24 hours up to 14 days. The estimated LD50 range was determined. Streptozotocin/ nicotinamide-induced female Mus musculus was administered to eval­uate the antidiabetic potential of MLEE. Six groups of mice were uti­lized, which included a healthy control group (aquades not induced), a negative control group (induced aquades), a positive control group (induced glimepiride 0.8 mg/kg BW), and three treatment groups with varying dosages of MLEE (induced; 0, 100, and 150 mg/kg BW). A semi-auto chemical analyzer was employed on days 0 through 31 to determine blood glucose levels. An analytical digital balance was utilized to calculate the body weight. With a total flavonoid concen­tration of 20.75%, MLEE incorporated alkaloids, flavonoids, pheno­lics, saponins, and tannins. MLEE demonstrated a significant effect in lowering blood glucose levels at a dose of 100 mg/kg BW (P<0.05). A significant positive correlation has been identified between body weight and blood glucose levels (P<0.05).
Aktivitas Antibakteri Ekstrak Temulawak (Curcuma xanthorrhiza) dan Bakteriosin Bifidobacterium longum terhadap Salmonella typhi Prakasita, Vinsa Cantya; Ilham, Jessika; Amarantini, Charis
SCISCITATIO Vol. 5 No. 1 (2024): Volume 5 Number 1, January 2024
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.51.147

Abstract

Demam tifoid merupakan penyakit infeksi yang disebabkan oleh Salmonella typhi yang menyerang sistem pencernaan. Infeksi Salmonella ini menyebabkan morbiditas dan mortalitas yang signifikan di seluruh dunia. Penyakit ini dapat diatasi dengan antibiotik, namun saat ini sudah banyak terjadi resistensi antibiotik sehingga penggunaannya kurang efektif. Temulawak merupakan salah satu tanaman yang digunakan sebagai obat tradisional di Indonesia yang memiliki banyak manfaat kesehatan. Bacteriocin memiliki aktivitas antimikroba terhadap bakteri patogen. Oleh karena itu, penelitian ini bertujuan untuk mengetahui aktivitas daya hambat ekstrak etanol temulawak dan bakteriosin Bifidobacterium longum terhadap pertumbuhan S. typhi. Ekstraksi temulawak dilakukan dengan metode maserasi dengan pelarut etanol. Skrining fitokimia dengan berbagai reagen dilakukan untuk mengetahui senyawa yang ada di dalamnya. Kadar total fenolik diukur dengan menggunakan persamaan regresi yang dibandingkan dengan asam galat. Bakteriosin B. longum diperoleh dengan cara menambahkan NaOH pada supernatan hasil panen dan memanaskannya pada suhu tinggi. Uji aktivitas daya hambat ekstrak etanol temulawak (EET) dan bakteriosin B. longum (BBl) dilakukan dengan metode difusi disk. EET mengandung alkaloid, saponin, terpenoid, flavonoid, dan fenol dengan kadar total fenol 30,73 ± 2,81 mg GEA/g. EET memiliki aktivitas daya hambat yang rendah hingga sedang, sedangkan BBl memiliki aktivitas daya hambat yang rendah terhadap S. typhi.
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9, No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Abstract

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.
PERSEPSI MAHASISWA PRODI BIOLOGI TENTANG IMPLEMENTASI MERDEKA BELAJAR KAMPUS MERDEKA DI SALAH SATU PERGURUAN TINGGI SWASTA INDONESIA Prakasita, Vinsa Cantya; Prasetyaningsih, Aniek; Prasetya, Heribertus Sigit
Jurnal Pendidikan Biologi Vol 15, No 2 (2024)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um052v15i2p180-186

Abstract

Abstrak. Merdeka Belajar-Kampus Merdeka (MBKM) merupakan kebijakan baru di bidang pendidikan di Indonesia. Pemerintah mengharapkan adanya peningkatan kompetensi lulusan, baik hard skills maupun soft skills serta sifat kapital intelektual yang kompetitif dan komperatif untuk menyambut globalisasi. Persepsi mahasiswa mengenai pemahaman dan kesiapan implementasi MBKM perlu diketahui sebagai masukan kepada pemangku kebijakan agar tujuan program dapat tercapai. Penelitian ini menggunakan pendekatan deskriptif-kuantitatif dan kuantitatif. Data diperoleh dari hasil Survei Implementasi MBKM 2021 yang dilakukan oleh Kementerian Pendidikan dan Kebudayaan Republik Indonesia terhadap mahasiswa Prodi Biologi di salah satu PTS Indonesia. Hasil analisis menunjukkan bahwa MBKM dapat menjadi solusi untuk meningkatkan hard skills dan soft skills mahasiswa. Bentuk kegiatan pembelajaran MBKM sesuai dengan kebutuhan lulusan Prodi Biologi di masa mendatang. Sebagai kesimpulan, pemahaman yang baik mengenai program MBKM dan dukungan dari pemerintah perguruan tinggi maupun orang tua akan mendukung terwujudnya tujuan dari program MBKM yaitu mencetak pemimpin masa depan yang unggul dan berkepribadian. Abstract. Freedom to Learn (MBKM) is a new policy of education in Indonesia. The government expects an increase in the competence of graduates, both hard skills and soft skills as well as the competitive and comparative nature of intellectual capital to face up globalization. Students' perceptions of understanding and readiness to implement MBKM need to be known as input to policy makers so that program objectives can be achieved. This research uses descriptive-quantitative and quantitative approaches. The data was obtained from the results of the 2021 MBKM Implementation Survey conducted by the Ministry of Education and Culture of the Republic of Indonesia on Biology Study Program students at one of the Indonesian private universities. The results of the analysis show that MBKM can be a solution to improve students' hard skills and soft skills. The form of MBKM learning activities is in accordance with the needs of future Biology Study Program graduates. In conclusion, a good understanding of the MBKM program and support from the university government and parents will support the realization of the objectives of the MBKM program, which is to produce future leaders who are superior and with good personalities.
Preliminary Selection of Potential Probiotic Lactic Acid Bacteria Isolated from Cocoa Beans (Theobroma cacao) Amarantini, Charis; Prakasita, Vinsa Cantya; Sema, Maria Trivonia
Biosfer: Jurnal Tadris Biologi Vol 12 No 1 (2021): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v12i1.7128

Abstract

This study aims to isolate and identify LABs that spontaneously grow fermented cocoa beans that have the potential as probiotics. The isolation was carried out selectively using an MRS agar medium supplemented with 1% CaCO3. LAB's potential as a probiotic was selected based on the acid resistance test, bile salt resistance test, and antimicrobial activity test. The results showed that three isolates of lactic acid bacteria, namely strains K1B1, K2T2, and K3B4 were tolerant of gastric acid's pH conditions (pH 2.5, pH 3, and pH 4). They also can survive the bile salts (0.3%, 0, 5%, and 1%) and have antibacterial characteristics. The three isolates were identified as Lactobacillus Plantarum species (ID 99.9%).
Antibacterial Activity of Dendrophthoe pentandra (L.) Leaves Extract Against Staphylococcus aureus and Escherichia coli Inggrid, Annabelle Marie; Aditiyarini, Dwi; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 2 (2024): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i2.30436

Abstract

Sapuran, Wonosobo is a popular area with several local tea plantations, which are infested by mistletoe, such as Dendrophthoe pentandra (L.) Miq. Mistletoe has been widely reported to have antibacterial activity due to its secondary metabolite content. Therefore, this study aimed to identify and explore the antibacterial activity of D. pentandra leaves extract against Staphylococcus aureus and Escherichia coli. The test samples were extracted using the maceration method, followed by phytochemical screening and total assay of flavonoids, phenols, and tannins. Antibacterial activity tests were carried out using disk diffusion, Minimum Inhibitory Concentration (MIC), and Minimum Bactericidal Concentration (MBC). The yield from the maceration method using 96% ethanol solvent was 8.78%, containing secondary metabolite compounds, including alkaloids, saponins, steroids, terpenoids, and flavonoids. The total flavonoid, phenol, and tanin content obtained were 4.09 ± 0.70 mg QE/g, 1.76 ± 0.16 g GAE/g, and 1.37 ± 0.14 mg TAE/g, respectively. In addition, the extract showed medium inhibition of S. aureus at a concentration of 400 and 525 mg/m, with no inhibitory effect against E. coli. The MIC and MBC from the microdilution method for S. aureus were at 4400 and 8800 mg/mL extract, while values of 28000 and 56000 mg/mL were obtained for E. coli. Based on these results, the inhibition activity of ethanol extract of D. pentandra against S. aureus was greater compared to E. coli.
Antibacterial Activity of Scurrula ferruginea (Roxb. Ex Jack) Leaves Extract Against Staphylococcus aureus and Escherichia coli Aditiyarini, Dwi; Sipayung, Nita Sonia; Prakasita, Vinsa Cantya
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30445

Abstract

The mistletoe (Scurrula ferruginea) is a parasitic plant obtaining nutrients and water from host plants at Nglinggo tea plantation, Kulon Progo, Yogyakarta. Despite the numerous benefits of Scurrula ferruginea leaf, substantiation regarding its pharmacological activity is still lacking. Therefore, this research aimed to identify Scurrula ferruginea species and obtain secondary metabolites with antibacterial activity. The experiment was carried out by extracting Scurrula ferruginea leaf using maceration methods with 96% ethanol, followed by qualitatively and quantitatively phytochemical screening, inhibitory zone test, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Several compounds, such as flavonoids, tannins, phenols, saponins, alkaloids, terpenoids, and steroids, were identified in the mistletoe extract. The results showed total flavonoid, phenolic, and tannin levels were 7.41 ± 0.38 mg QE/mg, 1672 ± 50.99 mg GAE/g, and 1.43 ± 0.15 mg TAE/g, respectively. Scurrula ferruginea leaf ethanolic extract showed the potential to form an inhibition zone against S. aureus in the moderate category at 525 mg/mL, but no significant activity was observed against E. coli. The MIC results of Scurrula ferruginea leaf ethanolic extract against S. aureus were 0.6%, and the MBC was at 2.4%. Furthermore, the MIC value against E. coli was 14%, and the MBC value was 28%. Based on these results, it could be concluded that Scurrula ferruginea leaf ethanolic extract showed high effectiveness in inhibiting and killing S. aureus bacteria compared to E. coli, with MIC and MBC values of 0.6% and 2.4%, respectively
Co-Authors Adrenalin, Sruti Listra Agnesia Endang Tri H. Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Agustina Viktoria Tae Ajiguna Ajiguna Anak Agung Gede Sugianthara Angelia Astria Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Aniek Prasetyaningsih Anjela Noya CHARIS AMARANTINI Charles Rangga Tabbu Christine Stefani Tamelan Dessy Imelda Nirmasari Siregar Dhira Satwika Djoko Rahardjo Djoko Rahardjo Dwi Aditiyarini Enjelin Anjung Susilowati Eunnike Adelina Fauziah, Ima Feblicya Palimbunga Ilham, Jessika Ima Fauziah Ima Fauziah Ima Fauziah Imanjati, Lynda Nugrahaning Inggrid, Annabelle Marie Irein Kusuma Angreini Jade Septhimoranie Jeffi Chandra Kukuh Madyaningrana Laurentia Henrieta Permita Sari Purba Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Lynda Nugrahaning Imanjati Maria Trivonia Sema Marlin Cindy Claudya Malelak Nadya Aprina Theodora Nita Sonia Sipayung Pepiana, Marlen Aviati Sarah Prasetya, Heribertus Sigit Priskila Melinia Wuni Relisantika Yulia Sinaga Sema, Maria Trivonia Septhimoranie, Jade Setiawan, Dwi Cahyo Budi Setya Harianto Sidna Artanto Sipayung, Nita Sonia Sitarina Widyarini Sitarina Widyarini Sruti Listra Adrenalin Sruti Listra Adrenalin Sruti Listra Adrenalin Susilowati, Enjelin Anjung Tara Inastu Kandarp Tati Aryani Theodora, Nadya Aprina Thomas Emanuel Manggotu Nahak Yovita Devina Yovita Devina