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All Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Majalah Obat Tradisional As-syifaa Jurnal Farmasi Journal Microbiology Science Jurnal Pengabdian Farmasi dan Sains
Nurung, Ayyub Harly
Fakultas Farmasi, Universitas Muslim Indonesia, Makassar
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Potential of Antibacterial Producing Rhizosphere Bacteria from Porang Roots (Amorphophallus muelleri Blume) against Gastrointestinal Pathogens Nuryanti, Siska; Nurung, Ayyub Harly; Akihito mr, Andi Al Kahfi
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1342

Abstract

Increasing antibiotic resistance in gastrointestinal pathogens such as Escherichia coli, Shigella dysenteriae, and Vibrio cholerae encourages the search for new antimicrobial agents. This study aims to evaluate the antibacterial potential of rhizosphere bacteria from porang roots (Amorphophallus muelleri Blume) against the three pathogens through in vitro tests. Isolation was carried out from rhizosphere soil using a serial dilution technique. Eleven isolates were characterized morphologically and tested for antibacterial activity using the agar diffusion method. Two selected isolates (IBRAM-2 and IBRAM-11) were fermented and extracted with ethyl acetate. Antibacterial activity was evaluated using the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests with the microdilution and drop plate methods, each with three replications and chloramphenicol as a positive control. The results showed an inhibition zone of 13.2–18.7 mm. The MIC value is 25,000 ppm, while the MBC value ranges from 25,000–50,000 ppm. Although it shows antibacterial activity, the high concentration required indicates weak antibacterial potential.
Molecular Analysis and Activities of Bioactive Compounds from Symbiont Bacteria Isolates Polycarpa aurata Herwin, Herwin; Alam, Gemini; Sartini, Sartini; Rahim, Abdul; Nurung, Ayyub Harly; Amirullah, Amirullah; Haq, Nasrul
Majalah Obat Tradisional Vol 31, No 1 (2026)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.105930

Abstract

Symbiont bacteria associated with Polycarpa aurata are known to produce secondary metabolites with diverse bioactive properties, including antibacterial, anticancer, antifungal, and antiprotozoal activities. This study evaluated the antibacterial and antibiofilm activities of symbiotic bacterial isolates obtained from the white morphotype of P. aurata collected from Barrang Lompo Island, Makassar City, Indonesia. The agar diffusion method and ELISA reader assay were employed for antibacterial and antibiofilm analyses, respectively. Four pure isolates were obtained and designated AQ2-1, AL2-3, AL2-4, and AL2-5. Molecular identification based on 16S rRNA gene sequencing indicated that isolate AQ2-1 corresponded to Pseudomonas aeruginosa strain AB18. This isolate exhibited a pronounced inhibition zone against Bacillus subtilis, with an optimal incubation period of 68 h for secondary metabolite production. Chemical analysis of the bioactive compounds from isolate AQ2-1 identified adenine riboside and bis(2-ethylhexyl) benzene-1,2-dicarboxylate as the major constituents. Adenine riboside exhibited the highest antibacterial activity, producing an inhibition zone of 12.2 mm against Escherichia coli ATCC 25922, while bis(2-ethylhexyl) benzene-1,2-dicarboxylate produced an inhibition zone of 10.8 mm against Staphylococcus epidermidis ATCC 14990. In the antibiofilm assay, adenine riboside achieved the highest inhibition at 77.09% against Staphylococcus aureus ATCC 25923, whereas bis(2-ethylhexyl) benzene-1,2-dicarboxylate inhibited Streptococcus mutans ATCC 25175 by 73.61%. These findings demonstrate that secondary metabolites produced by the AQ2-1 isolate of symbiont bacteria from P. aurata possess strong antibacterial and antibiofilm activities and may serve as promising candidates for future antimicrobial development.
Co-Authors Abdul Rahim Adnan, Afdina Putri Akihito mr, Andi Al Kahfi Ambon, Nur Intan Amirullah -, Amirullah Asnita Asnita Bayu Putra, Bayu Dewi Kartikawati Paramita Erwing, Erwing Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Fitriana Gemini Alam haq, Nasrul Herwin Herwin Herwin Herwin Herwin, Herwin Massaressung, Andi Musfatir, Harvanita Muzakkir Baits, Muzakkir Naim, Nurul Khatimah Nurul Khatimah Naim Putra, Aryadi Surya R. Rusli Rachmat Kosman Rachmat Kosman, Rachmat Rahmadhani, Paramita Rahman, Safriana Ririn Ririn Rusli Safriani Rahman, Safriani Sandyna, Muh. Ivan Saputra, Muh. Satrio Surya Sartini Sartini Siska Nuryanti, Siska Sitti Amirah Sri Herwiyanti, Sri Tadjuddin Naid Umaiyah, Ummi Putri Widowati, Fairuz Dyah Yoga Yuniadi