Article Per Year (5 Year)
p-Index From 2020 - 2025
2.012
P-Index
This Author published in this journals
All Journal Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Majalah Obat Tradisional Science and Technology Indonesia Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Jurnal Penelitian Pendidikan IPA (JPPIPA) Biota JURNAL BIDAN CENDRAWASIH PALU Jurnal Pengabdian kepada Masyarakat Narra J Basic and Applied Nursing Research Journal Jurnal Kedokteran Hewan Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Gemini Alam
Faculty of Pharmacy, Hasanuddin University, Makassar.
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

Published : 17 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 17 Documents
Search

 1   2 
SKRINING DAN ANALISIS KLT-BIOAUTOGRAFI SENYAWA ANTIMIKROBA BEBERAPA EKSTRAK SPONS ASAL PERAIRAN LAUT PULAU BARRANG LOMPO, SULAWESI SELATAN Yulianty, Risfah; Rante, Herlina; Alam, Gemini; Tahir, Akbar
Majalah Obat Tradisional Vol 16, No 2 (2011)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (166.514 KB) | DOI: 10.14499/mot-TradMedJ16iss2pp88-94

Abstract

Spons merupakan salah satu invertebrata filum Porifera yang menghasilkan senyawa aktif dengan berbagai variasi struktur dan salah satu aktivitas biologinya adalah sebagai antimikroba. Penelitian ini bertujuan untuk mengetahui ekstrak spons yang dapat menghambat pertumbuhan mikroba dan potensi senyawa aktif antimikroba dari spons asal perairan Pulau Barrang Lompo. Mikroba uji yang digunakan adalah Escherichia coli, Salmonella typhii, Staphylococcus aureus, dan Candida albicans. Bahan uji diperoleh dengan maserasi 14 buah sampel spons dengan metanol yang dilanjutkan dengan partisi berturut-turut menggunakan kloroform dan metanol, selanjutnya dilakukan uji KLT-bioautografi terhadap ekstrak yang aktif. Hasil penelitian menunjukkan bahwa ekstrak metanol dari sampel spons dengan kode BRLP-009 dan BRLP-010 mempunyai aktivitas paling potensial sebagai antimikroba.
Skrining Senyawa Antiinfeksi dari Spons yang Dikoleksi dari Bunaken, Manado Astuti, Puji; Alam, Gemini; Pratiwi, Sylvia; Hertiani, Triana; Wahyuono, Subagus
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 2 (2003): June 2003
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.243 KB) | DOI: 10.24002/biota.v8i2.2883

Abstract

The emergence of drug resistant and multidrug-resistant microbes as well as the lack of any current chemotherapy augmented the necessity to search for new and better anti-infective drug.. This study was aim to screen potential antiinfective extracts of sponges collected from Bunaken, Manado and to report on their antibacterial and antifungal properties. Testing for antiinfective agents was conducted using dilution method. Nutrient Agar was used as the testing media and nutrient broth for the inoculation of microorganisms. Staphylococcus aureus, Escherichia coli and Salmonella typhi were used as the testing bacteria and Candida albicans as the testing fungi. Chloramphenicol was used as positive control for anti bacterial activity and Ketokonazol for antifungi. The two acetone extracts tested (MD-01aceton and MD-02aceton), all of them showed inhibition activities. Following partition with chloroform and methanol, all chloroform extracts inhibited the growth of both bacteria and fungi. These extracts then are considered to be potential candidates for further isolation and characterization as antiinfective agents.
Pengaruh Umur Dan Pekerjaan Ibu Terhadap Kandungan Antioksidan Asi Enggar; Alam, Gemini; Maddeppungeng, Martira
Jurnal Bidan Cendrawasih Palu Vol 1 No 1 (2019): MARET
Publisher : Akademi Kebidanan Palu Sulawesi Tengah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Antioxidant is a compounds which can protect a baby from an oxidative stress and free radical hazard. Breast milk contains antioxidant and represents is the best food for a baby. This research animed at investigating the effect of women’s age and occupation on the breast milk antioxidant. The research used the cross sectional design with of 24 samples of the breastfeeding women who were selected using the purposive sampling technique. The respondents who were taken their breast milk samples were in healthy condition and were asked to become volunteers by contributing their breast milk in the sake of the research. The samples taken were the women who breastfed and had the babies ≤ 6 months old and fulfilled the inclusive and exclusive criteria. Oneway ANOVA analysis was used to perceive the effect of the women’s age and occupation on breast milk antioxidant. The research result indicates that the respondents’ age based on statistic test results is obtained the score of p= 0,563 ˃α=0,05 meaning that there is not significant effect between women’s age and breast milk antioxidant. The Respondent's occupation indicated the score of p= 0,055 ˃α=0,05 which means that there is no significant effect between the occupation and breast milk antioxidant. It is concluded that there is no effect of the women’s age and occupation on breast milk antioxidant. It is expected that the women who work outdoors can still give the breast milk. The women who can not give their breast milk directly can still give their breast milk by way squeezing.
Validated Chromatography Method for Analysis of Formaldehyde Migration Levels in Bottled Drinking Water Ramadhani, Nur; Yulianty, Risfah; Rifai, Yusnita; Aswad, Muhammad; Alam, Gemini; Naid, Tadjuddin; Kasim, Athia Kurnia
Science and Technology Indonesia Vol. 9 No. 3 (2024): July
Publisher : Research Center of Inorganic Materials and Coordination Complexes, FMIPA Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/sti.2024.9.3.660-668

Abstract

Polyethylene terephthalate (PET) is utilized in the containment of potable water, nonetheless, it is susceptible to impairment due to temperature and duration of storage. Migration of formaldehyde from PET arises as a consequence of thermal deterioration. This study aims to ascertain the extent of formaldehyde transference in PET-contained drinking water of varying condition, subjected to diverse temperature setting and storage durations. An examination into the levels of formaldehyde migration in polyethylene terephthalate (PET) bottled drinking water was effectively carried out employing a validated high-performance liquid chromatography (HPLC) technique. A total of 162 water samples from three different PET bottled water brands sourced from supermarkets in Palu City, Central Sulawesi, Indonesia, underwent analysis. The formaldehyde assessment was executed utilizing a mobile phase composition of methanol:acetonitrile:water (48:12:40 v/v) for 12.97 minutes at a flow rate of 0.8 mL/minute on the 1st day, the 14th day, and the 42nd day while stored at room temperature and exposed to sunlight. Formaldehyde, lacking effective chromophoric entities and not readily ionizable, necessitated derivatization with 2,4-dinitrophenylhydrazine before HPLC analysis. The findings of the study demonstrated exceptional method linearity within the concentration span of 0.5 to 1321 µg/mL, exhibiting a commendable correlation coefficient of 0.999. The limit of detection (LOD) and quantification (LOQ) were determined to be 17.33 and 57.76 µg/mL, correspondingly. Precision evaluations displayed average intraday and interday values of 128.46±3.48% and 103.19±0.12%, respectively (%RSD < 2/3 CV Horwitz ratio). The intraday and interday %recovery values were computed at 97.22±3.48% and 96.83±0.14% (n = 6). The scrutiny of formaldehyde concentrations in the water samples unveiled the absence of formaldehyde migration.
Piper crocatum and Aloe vera Difference Effectiveness in Complementary Treatment of Perineal Wounds Mubayyina, Firdaus; Alam, Gemini; Usman, Andi Nilawati; Ahmad, Mardiana; As’ad, Suryani; Raya, Indah
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.409

Abstract

Indonesia, with a maternal mortality rate (MMR) of 305 per 100,000 live births, is directly caused by post-partum hemorrhage (30.3%) and hypertension (27.1%). At the same time, post-partum infection ranks second as a contributor to maternal death after bleeding. Data from the Indonesian Demographic Health Survey (IDHS) showed that the incidence of perineal laceration or rupture was experienced by women who gave birth vaginally (75%). The prevalence of women who experience perineal tears is in the 25-30 years (24%) and women aged 32-39 years (62%). This study aimed to examine the differences in the effectiveness of Piper crocatum and Aloe vera in the complementary treatment of perineal wound healing in post-partum mothers. This study was a quasi-experimental pretest-posttest control group design with a total sampling method with a ratio of 1:1:1 for as many as 30 post-partum mothers with grade I and II injuries according to inclusion criteria with Intervention group red betel stew, aloe vera, and the Control group with perineal injuries at Gunung Sari Health Center and Sesela Health Center. The data observed were the degree of perineal wound healing using the REEDA scale (Redness, Edema, Ecchymosis, Discharge, and Approximation). The results showed differences in the effectiveness of Piper crocatum and aloe vera on perineal wound healing. The average results of the Mann-Whitney statistical test were on the 5th and 7th days (p-value< 0.005). This study shows that Piper crocatum can accelerate the healing of perineal wounds and become an alternative, complementary therapy in perineal wound healing.
Comparison of the Effectiveness of Piper Crocatum and Sour Turmeric Acid for Healing Time Lestari, Dewi; Usman, Andi Nilawati; Alam, Gemini; Hadju, Veni; Husin, Farid; Sartini, Sartini
Biota Vol 15 No 1 (2022)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/jb.v15i1.412

Abstract

Maternal mortality (MMR) is still 359 per 100,000 live births, and the expected target is still far from what was proclaimed in 2030, the number of 70 per 100,000 live births. Based on data from WHO (Word Health Organization), in 2015, the deaths were around 2.7 million cases caused by perineal injuries. According to IDHS data (Indonesian Demographic and Health Survey), in 2017, the maternal mortality rate (MMR) was 305 per 100,000 live births caused by an infection in the perineal wound. The total maternal mortality rate (MMR) in various provinces in Indonesia has decreased from 4,226 to 4,221. In 2019 the maternal mortality rate (MMR) was most contributed by bleeding (30.3%) and postpartum infection (5%). This study aimed to determine the differences in the effectiveness of red betel and turmeric acid in the complementary wound healing treatment in postpartum women. This study was a quasi-experimental pretest-posttest control group design with a total sampling method by giving three intervention and control groups, as many as 30 postpartum women with perineal wounds grades I and II according to the inclusion criteria with the group given red betel, tamarind, and turmeric. Control group at Anutapura General Hospital Palu. Perineal wound care using the REEDA scale (Redness, Edema, Ecchymosis, Discharge, and Approximation). The results showed a difference in the effectiveness of red betel and turmeric acid on perineal healing. The average Mann-Whitney statistical test results were on the 5th and 7th days (P-Value < 0.005). This study shows that Piper crocatum can accelerate perineal wound healing and become an alternative for complementary therapy and perineal wound healing.
Study of Molecular Docking, Molecular Dynamics, Pharmacokinetics and Toxicity Prediction: Compounds from Nigella sativa Linn, Andrographis paniculata Nees, and Propolis as Inhibitors of Mycobacterium tuberculosis Growth Asarini; Abdillah, Syamsudin; Sani, Yulvian; Alam, Gemini
Basic and Applied Nursing Research Journal Vol 5 No 2 (2024): Basic and Applied Nursing Research Journal (BANRJ)
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/banrj.05.02.02

Abstract

Background: Tuberculosis (TB) is caused by *Mycobacterium tuberculosis*, and Multiple Drug-Resistant Tuberculosis (MDR-TB) arises from resistance to first-line treatments like Rifampicin and Isoniazid. Since current TB medications have been used for over four decades, discovering new drug candidates is critical. This research focuses on herbal compounds—Black cumin (*Nigella sativa*), Sambiloto (*Andrographis paniculata*), and propolis—as potential inhibitors of *M. tuberculosis* by targeting DHFR. The objective of the study is to predict the activity of these herbal compounds prior to in vitro and in vivo testing. Methods: This study employed computational tools, including Molegro Virtual Docking (MVD) and Molecular Dynamics (MD), to assess the interactions of the herbal compounds with DHFR (PDB ID: 2CIG). Pharmacokinetic predictions were also conducted to evaluate the absorption and toxicity of the compounds. Results: Molecular docking and MD simulations indicated that Andrographolide, Thymoquinone, and Caffeic Acid Phenethyl Ester effectively inhibited the growth of *M. tuberculosis*. The analysis revealed favorable binding interactions and conformational changes in DHFR, with significant activity observed for Thymoquinone. Conclusion: This study suggests that Andrographolide, Thymoquinone, and Caffeic Acid Phenethyl Ester may serve as promising candidates for further development as anti-tuberculosis drugs. Subsequent in vitro and in vivo studies are warranted to validate their therapeutic potential.
Scoping Review: Study of Herbs Consumption for Self-Medication in Indonesia 2019-2022 Azzahra, Fatimah; Astuti, Ayun Dwi; Arifin, Bustanul; Alam, Gemini
Majalah Obat Tradisional Vol 29, No 3 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.94091

Abstract

Indonesia is a country that has high biodiversity and is rich in traditional medicine from natural ingredients or herbs. One use of these natural ingredients is as a treatment for self-medication. Many previous studies have been carried out but none has reviewed it systematically, especially during the Covid-19 pandemic. This research was carried out systematically using the PRISMA statement approach using the Google Scholar data bank. Out of 2,214 papers in the 2019-2022 period,  45 articles/scientific papers papers met the criteria, including inclusion and exclusion criteria, for a description/profile of the use of natural ingredients or herbs as self-medication for traditional medicine. The central region of Java (D.I.Y Yogyakarta and Central Java) is the region with the largest number of articles. The distinctiveness of Indonesia's approach to self-medication lies in its utilization of various natural elements to address similar ailments, which can be attributed to geographical factors, cultural beliefs, and diverse knowledge across different regions. Meanwhile, Covid-related studies were very limited due to the availability or the preference for chemical self-medication.
Resistance status of Aedes mosquitoes as dengue vectors and the potential of plant larvicides from Indonesia for biological control: A narrative review Kasman, Kasman; Ishak, Hasanuddin; Alam, Gemini; Amiruddin, Ridwan; Hastutiek, Poedji; Arsin, Andi A.; Nasir, Sudirman; Ridha, Muhammad R.; Wahid, Isra
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1819

Abstract

Dengue fever remains a major public health threat in Indonesia, exacerbated by rising insecticide resistance in Aedes aegypti. Strategies relying on chemical insecticides, while initially effective, have led to widespread resistance in mosquito populations. This resistance is particularly pronounced in areas such as Java, where the extensive use of insecticides, including organophosphates and pyrethroids, has been documented. To address this challenge, one promising alternative is the utilization of biolarvicides derived from local Indonesian plant materials. Biolarvicides are environmentally friendly, safe, and have the potential to mitigate the adverse impacts associated with chemical insecticides. Numerous studies have explored the larvicidal properties of indigenous plants native to Indonesia, demonstrating their efficacy against A. aegypti. The aim of this study was to examine insecticide resistance in Aedes mosquitoes across Indonesia, highlighting geographical variations and underexplored regions, and exploring plant-based biolarvicides as sustainable alternatives.  Biolarvicides derived from native Indonesian plants could be eco-friendly alternative for dengue vector management. Their integration into existing control strategies could significantly enhance efforts to control dengue while reducing the environmental and health risks posed by chemical insecticides.
THE ANTIMYCOBACTERIAL POTENTIAL OF SAMBILOTO (Andrographis paniculata Nees) EXTRACT AGAINST Mycobacterium tuberculosis H37Rv WITH Microscopic-Observation AND Drug-Susceptibility (MODS) METHODE Asarini; Abdillah, Syamsudin; Sani, Yulvian; Alam, Gemini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.8626

Abstract

Andrographis paniculata, commonly known as sambiloto, is empirically used for various treatments, including its ability to inhibit the growth of Mycobacterium tuberculosis (M. tuberculosis). This study aims to evaluate the growth inhibition activity of M. tuberculosis strain H37Rv using sambiloto herb extract (Andrographis paniculata). The sambiloto extract was prepared using the maceration method. M. tuberculosis was grown on MODS medium, and the toxicity of sambiloto (A. paniculata) was analyzed using pharmacokinetic prediction studies (pkCMS). The results showed that at concentrations of 0.255 mg/ml, 1.275 mg/ml, and 2.55 mg/ml of sambiloto extract, M. tuberculosis growth occurred on days 7 to 14. Further observations were made until day 28, and it was found that starting at a concentration of 6.375 mg/ml, sambiloto extract did not show M. tuberculosis growth in MODS medium. The positive control, isoniazid, did not show bacterial growth, while the negative control showed extensive bacterial growth on day 12. The determination of M. tuberculosis growth was based on microscopic observations of the bacterial colonies, focusing on cord formation. In conclusion, this study, the use of an extract with a concentration of 6.375 mg/ml in the MODS method showed no growth of Mycobacterium tuberculosis, indicating that the extract is effective in inhibiting bacterial growth at this concentration. The online pkCSM test conducted in this study showed that the extract used is not cytotoxic, meaning that the extract is safe for body cells and does not cause cellular damage. Therefore, it has the potential to be an adjunct therapy in the treatment of tuberculosis.
Co-Authors . Lukman . Mufidah . Subehan . Syamsudin Abd Halim Umar Ahmad, Mardiana Aisyah Fatmawaty Akbar Tahir, Akbar Andi Nilawati Usman Arsin, Andi A. Arsyad, Muhammad Aryadi Asarini Astuti, Ayun Dwi As’ad, Suryani Bustanul Arifin Dewi Lestari Dewi Purwaningsih Djabir, Yulia Yusrini Elly Wahyudin Enggar Fatimah Azzahra Gatot S Lawrence Haeriah Haeriah, Haeriah Hasanuddin Ishak Herlina Rante Husin, Farid Indah Raya Isra Wahid Kasim, Athia Kurnia Kasman Kasman Latifah Rahman Lukman Hakim M Rusdi Mangilep, Muhammad Agung Ady Marianti A Manggau Martira Maddeppungeng, Martira Marwati Marwati Matias N Kolobani Mubayyina, Firdaus Muh. Akbar Bahar Muh. Nasrum Massi Muhammad Aswad Natsir Dijde, Nana Juniarti Natsir Djide Nursamsiar Poedji Hastutiek Pratiwi, Sylvia Puji Astuti Putri, Dwi Anggara Ramadhani, Nur Ridha, Muhammad R. Ridwan Amiruddin Risfah Yulianty Riska Nufika Ritha Pratiwi Sami, Fitriyanti J. Sardjiman S Sartini Sartini Sitarina Widyarini Subagus Wahyuono Sudirman Nasir Syamsu Nur, Syamsu Tadjuddin Naid Triana Hertiani Veni Hadju Wahyu Hendrarti Yulvian Sani Yusnita Rifai, Yusnita