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Jurnal Teknologi Laboratorium
Published by Poltekkes Kemenkes Yogyakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Education,
Chemistry Other
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Articles 97 Documents
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Turkey Ozudogru, Osman; Yerlikaya, Emrah; Alayunt, Naci Omer; Çambay, Zafer
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.266

Abstract

The purpose of this study is to investigate the healing effect of Favipiravir used in pre-intensive care treatment of patients diagnosed with COVID-19 in order to elucidate the pathogenesis and complications of coronavirus. The data regarding the clinical findings of the patients in the hospital information system and the biochemical parameters made standard in the treatment/follow-up of COVID 19 were taken from the system and evaluated retrospectively. In addition, it was examined as a whole with mild, moderate and severe pulmonary involvement compared to CT findings. Hemogram, coagulation and biochemistry parameters used in the diagnosis and follow-up of COVID-19 were evaluated. SPSS 22.0 statistics program for Windows was used in statistical analysis to evaluate the data obtained from patient files and hospital information system. There is no definitive treatment protocol within the scope of treatment. Drug studies are currently ongoing. In this study, the first clinical findings, treatment types and recovery times of patients diagnosed with COVID-19, the healing effect of favipiravir used before intensive care were determined. Between group 1 (those who started treatment within 0-5 days) and group 2 (those who started treatment within 6-10 days), after 5 days of favipravir treatment, when serum parameters were compared, favipravir treatment was statistically significantly lower in the first group that was started early, WBC, Neutrophil, Creatine, CK, CRP, D-Dimer, PCT, LDH. By collecting the data obtained as a result of the research, early deaths can be prevented worldwide. Our study recommending alternative treatment approaches is important for the protection of patients' quality of life. In this study, when all biochemical markers were evaluated together, it was evaluated that starting Favipiravir treatment early was beneficial in treating COVID-19 disease.
A simple method for isolation of rest of trypsinized stem cells with magnetic beads Bhatia, Sudhir
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.268

Abstract

Till now, there is no laboratory in the world, which makes thoughts that there may be still stem cells remaining in the flasks after the trypsin treatment. The user checks the presence of stem cells during trypsinization with the microscope whether the whole population of stem cells has been collected. Magnetic beads are being used around the world to isolate different kinds of cells like CD4, CD8, CD34, and other cells. One of the advantages of magnetic isolation is that they can be used to isolate cells from solutions containing even a low number of targeted cells. We conducted the experiments to see whether all remaining MSC can be obtained from a trypsin-treated flask with magnetic beads isolation. During the magnetic isolation with CD90 specific Magnetic beads, it was found under the microscope that there are huge numbers of cells being attached to beads. The results indicate that there are still a huge number of rest cells in the trypsinized flask but most of the users think that they have isolated all cells, which is not true and they are throwing away valuable stem cells. The magnetic beads can be used to isolate the rest of MSC because for many applications, there is a need of the largest number of stem cells for conducting studies like flow cytometry, molecular testing along with pre-clinical and clinical studies.
Conserved B-cell epitope identification of envelope glycoprotein (GP120) HIV-1 to develop multi-strain vaccine candidate through bioinformatics approach Kharisma, Viol Dhea; Ansori, Arif Nur Muhammad; Posa, Gabrielle Ann Villar; Rizky, Wahyu Choirur; Permana, Sofy; Parikesit, Arli Aditya
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.274

Abstract

Acquired immune deficiency syndrome (AIDS) has been identified from US patients since 1981. AIDS is caused by infection with the human immunodeficiency virus type 1 (HIV-1) which is a retrovirus. HIV-1 gp120 can be recognized by the immune system because it is located outside the virion. The conserved region is identified in gp120, and it is recognized by an immune cell which then initiates specific immune responses, viral mutation escape, and increase vaccine protection coverage, a benefit derived from the conserved region-based vaccine design. However, previous researchers have little knowledge on this conserved region as a target for vaccine design. This paper explains how the conserved region of gp120 HIV-1 is a major target for vaccine design through a bioinformatics approach. The conserved region from gp120 was explored as a vaccine design target with a bioinformatics tool that consists of B-cell epitope mapping, vaccine properties, molecular docking, and dynamic simulation. The peptide vaccine candidate of B5 with the gp120 HIV-1 conserved region was found to provoke B-cell activation through a direct pathway, produce specific antibody, and increase protection from multi-strain viral infection.
Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa Kartika, Aprilia Indra; Fitria, Meutia Srikandi; Oktaviola, Vanny
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.276

Abstract

Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA is needed to determine the type of bacterial contaminants in C. racemosa. The isolates of C. racemosa were cultured in HIA, BAP, and BHI media. Bacteria from BHI media were isolated by DNA, PCR for 16S rRNA gene, and sequencing. Bacteria isolate C. racemosa was found to have the α-hemolytic ability in BAP media. The sequencing analysis showed that the three bacterial colonies of C. racemosa isolate had high similarity with V. parahemolyticus, Caldalkalibacillus mannanilyticus, and Exiguobacterium profundum.
Air-Dried and Wet Fixation on Fine Needle Aspiration Biopsy (FNAB) Specimen Nuroini, Fitri
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.279

Abstract

Kualitas pewarnaan Diff-Quick bergantung pada beberapa faktor diantaranya adalah metode fiksasi yaitu fiksasi kering dan fiksasi basah. Kedua metode tersebut memiliki kekurangannya masing-masing, fiksasi kering dapat menyebabkan pecahnya sel sedangkan fiksasi basah lebih memakan waktu dan biaya dalam pengerjaannya, namun fiksasi kering lebih sering digunakan karena lebih cepat dan murah. Tujuan penelitian untuk membandingkan hasil pewarnaan Diff-Quick sediaan sitologi yang difiksasi dengan metode fiksasi kering dan metode fiksasi basah. Jenis penelitian adalah penelitian eksperimen. Sampel diperoleh dari RS K.R.M.T. Wongsonegoro Semarang berjumlah 36 sediaan yang dibagi menjadi tiga kelompok yaitu kelompok kontrol, kelompok fiksasi kering, dan kelompok fiksasi basah. Ketiga kelompok tersebut diwarnai dengan Diff-Quick. Pewarnaan sediaan fiksasi kering memberikan hasil 4 sediaan kurang baik, 5 sediaan baik, dan 3 sediaan sangat baik sedangkan pewarnaan sediaan fiksasi basah memberikan hasil 0 sediaan kurang baik, 8 sediaan baik, dan 4 sediaan sangat baik. Hal ini menunjukkan hasil pewarnaan sediaan yang difiksasi basah memiliki kualitas yang cenderung lebih baik dan lebih konsisten dibanding hasil pewarnaan sediaan fiksasi kering. Meskipun secara statistik melalui uji Post-Hoc menunjukkan tidak ada perbedaan, kualitas pewarnaan Diff-Quick sediaan sitologi yang difiksasi dengan metode fiksasi basah lebih baik dari pada fiksasi kering.
Digital communication in health promotion in handling tuberculosis sputum Makalew, Linda Augustien; Rambi, Elne Vieke; Makaminan, Muhammad Ali
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.285

Abstract

The Covid-19 pandemic that has caught the attention of not only Indonesia but the rest of the world. The 3M movement, namely using masks, washing hands and keeping a distance to minimize the transmission of tuberculosis, is very useful for the eradication of TB tuberculosis. However, this is a challenge in itself for microscopic officers at the Microscopic Reference Center (MRC) in education and handling of sputum for tuberculosis patients who are currently being treated. The purpose of this study was to test VisKomLAM 1, 2, 3 and 4 in bridging communication between officers in sputum management education for tuberculosis sufferers at each examination during the Covid-19 pandemic. The research locations took MRC Tuminting, Wawonasa and Tikala Baru in Manado City and MRC Telaga in Gorontalo District. Analysis with paired t test showed a significance below 5%, which means that VisKomLAM 1, 2, 3 and 4 can bridge education from officers to tuberculosis sufferers in the Covid-19 pandemic era. It is recommended that health workers always strive to promote tuberculosis health in achieving tuberculosis elimination by 2050
Multi drugs resistance to Diabetes Mellitus patients with tuberculosis in Manado City Rambi, Elne Vieke; Sukandar, Dyan R; Makalew, Linda Augustien; Tomastola, Yohanis; Konoralma, Ketrina
Jurnal Teknologi Laboratorium Vol 10 No 1 (2021): inpress
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v10i1.286

Abstract

Diabetes mellitus (DM) with pulmonary tuberculosis (TB) is an infectious disease if not educated regularly, there will be a high risk of drug resistance and even some anti-tuberculosis drugs. This study aims to identify anti-tuberculosis drug resistance in DM patients with TB in Manado City. The population in the study types 2 DM patients as amount 80 patients. Based on TCM/GenExpert examination from 47 respondents, there were 17 respondents positive multi drugs resistance rifampicin (RR). Sampling taking based on inclusion criteria, i.e., have had type DM for five years, had suffered TB MDR RR based on GenXpert examination as much as 17 respondents followed in the resistance test with Sputum TB culture and MGIT method. The result of the study showed that MDR DM-TB with MGIT method as followed is obtained from 17 samples, six samples (35.30%) resistance INH 0.4 mg and 1 sample (5.88%) MDR canamycin, and still sensitive INH 0.4 mg and camaycin is ten samples (58.82%). This study results could be used to program planning of prevention and controlling efforts TB-DM in this treatment obedience and regimen anti-tuberculosis medicine for MDR-TB patients.

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