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Chymase Level in Dengue Virus Infection With or Without Positive Non-Structural 1(NS1) Kasiyati, Menik; Nugraha, Jusak; Kahar, Hartono
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Thrombocytopenia, leukopenia, and monocytosis are laboratory parameters in the diagnosis of dengue virus infection. In addition to monocyte cells, mast cells also play a role in the innate immune response, where degranulation of mast cells will occur, which will secretion the active vaso mediator, Chymase. Chymase has a role in increasing vascular permeability resulting in plasma leakage in patients with dengue virus infection to determine the number of platelets, leukocytes, monocytes and chymase levels in patients with dengue infection in the acute phase. The platelet count mean in NS1 (+) was 132,140 cells / mm3 and the platelet count in the NS1 group was (-) 176,000 cells / mm3. The mean leukocytes NS1 (+) showed results of 4,350 cells / mm3 and NS1 (-) 5,250 cells / mm3. The mean monocyte NS1 (+) monocyte count was 8.26%, and NS1 (-) group was 8.76%. There were no significant differences in platelet counts, leukocytes and monocytes between NS1 (+) and NS1 (-) (P value> 0.05). The mean Chymase NS1 (+) 23.48, NS1 (-) 23.05 ng / mL and the control group 1.47ng / mL.

The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freundâ€™s Adjuvant Buana, Dwi Candra; Sudiana, I Ketut; Nugraha, Jusak
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.

Total Lymfosit Count (TLC) with CD4 in HIV/AIDS Patients at Kupang Wuan, Adrianus Ola; Herlinalt Gloria Banunu, Ayorince; Kambuno, Norma Tiku
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Human Immunodeficiency Virus (HIV) is a retrovirus originating from the retroviridae family of the genus lentivirus that infects and damages cells that have a molecule Cluster of Differentiation 4 (CD4), especially T lymphocytes that have receptors with high affinity for HIV. Total lymphocyte count / TLC has been proposed as an alternative guide to CD4 in limited health facilities. This study aims to determine the correlation between Total Lymphocyte Count (TLC) and CD4 in HIV/AIDS patients in the W.Z. Johannes Kupang hospital. The type of this research was observational analytic with a cross-sectional design. The study was conducted on 121 samples of patients who performed CD4 examination and Total Lymphocytic Count (TLC) in the laboratory of W.Z.Johannes Kupang Hospital. The Spearman correlation test shows a significance value of 0,000 with a Spearman correlation value of 0.799. Based on the results of this study it can be concluded that there is a significant correlation between Total Lymphocyte Count and CD4 and shows the direction of positive correlation with a very strong relationship, where the increase in the number of Total Lymphocyte Count is in line with the increase in CD4 counts.

The potency of green grass jelly extract (Premna oblongifolia Merr) as antihyperlipidemia towards aorta histopathology representation of rat (Rattus norvegicus) induced with high fatty diet (HFD) Winthoko, Eka Nora Vitaloka Aprilia Putri; Roosdiana, Anna; Pratama, Dyah Ayu Oktaviani A; Nugraha, Jusak; Purwanta, Marijam; Rifaâ€™I, Muh Husni; Rendy, Achmad Nur
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.174

Green grass jelly (Premna oblongifolia merr) is a plant containing fiber and chlorophyll which can lower cholesterol and triglyceride levels. This study have aims to investigate the potency of green grass jelly extract (Premna oblongifolia Merr) to prevent hyperlipidemia. The animal mode used for this study is male Rattus norvegicus, Wistar strain, the age of 8 weeks, and weight of 200 g which is divided into 5 groups of treatment namely group Kn (negative control), Kp (positive control), Kp1, Kp2, and Kp3 induced with HFD and green grass jelly extract at a dose of 5.27 g/ kg BW/ daily, 8.43 g/ kg BW/ daily, 9.37 g/ kg BW/ daily. The data of infiltrating fatty cells and macrophage on aorta histopathology was analyzed by description. This research showed that the treatment of green grass jelly extract (Premna oblongifolia Merr) to animals of hyperlipidemia model reduced infiltration fatty cells and macrophage. The conclusion of this study was the green grass jelly extract was able to prevent an increase of fatty cells and macrophage infiltration of rat (Rattus noervegicus) induced with HFD on dose 9.37 g/ kg/ BW/ daily.

TUBERKULOSIS (TB) ADALAH SUATU P GAMBARAN INDURASI DAN EKSPRESI CD163 SEBAGAI MARKER AKTIVASI MAKROFAG M2 PADA TIKUS RATTUS NORVEGICUS YANG DIIJEKSI DENGAN PURIFIED PROTEIN DERIVATE (PPD) lestari, veni ayu
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1) (inpress)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.179

Abstrak               Tuberkulosis (TB) adalah suatu penyakit infeksi menular yang disebabkan oleh Mycobacterium tuberculosis (MTB), yang dapat menyerang beberapa organ terutama paru-paru. Untuk mengidentifikasi kasus TB mulai dikembangkan Tuberculin Skin Test (TST). Tes kulit tuberkulin, yang melibatkan pemantauan reaksi kekebalan terhadap suntikan Purified Protein Derivative (PPD). Tujuan penelitian ini adalah untuk mengamati indurasi dan ekspresi CD163 sebagai marker aktivasi makrofag M2 pada tikus. Metode penelitian ini bersifat eksperimental yang akan disajikan dalam bentuk gambar secara deskriptif. Sampel penelitian ini adalah tikus (Rattus norvegicus) dengan jumlah 7 ekor yang dibagi menjadi 2 yaitu 1 ekor untuk kontrol dan 6 ekor untuk perlakukan. Penelitian ini dilakukan di Laboratorium Biokimia Universitas Airlangga pada bulan April 2019. Hasil penelitian ini adalah pada tikus perlakuan terjadi indurasi setelah 42 dan 72 jam setelah diinjeksi Purified Protein Derivative (PPD) dengan hasil ? 5 mm yang dapat diartikan sebagai indurasi positif sehingga dapat disimpulkan makrofag teraktivasi dan hasil pemeriksaan imunohistokimia pada jaringan kulit tikus dengan mikroskop didapatkan hasil dari 6 sampel perlakuan 3 sampel diantaranya menunjukkan adanya ekspresi CD163 ditandai dengan adanya sel radang dan granuloma. Maka dapat disimpulkan bahwa PPD yang diinjeksikan pada tikus dapat mengaktifkan sel radang dan granuloma serta mengekpresikan CD163 pada jaringan kulit tikus.     Kata Kunci : PPD, Tikus, CD163

The potential of traditional balinese spices against the growth of Salmonella sp in vitro Jirna, I Nyoman; Sudarmanto, I Gede; Kurniawan, Surya Bayu; Ratih, Gusti Ayu Made; Rasyid, Burhannuddin
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.200

Prevention by utilizing medicinal plants can be used as an alternative treatment. This study aims to identify active antimicrobial substances and test the antimicrobial potential of traditional Balinese spices, namely Basa Selem, Basa Gede, Basa Wangen, Basa Bawang Jahe, and Basa Rajang against Salmonella sp bacteria in vitro. The True-experimental method with Posttest only-control design was used in this study, by intervening in the treatment group as well as the presence of positive and negative controls. The maceration method with 96% EtOH solvent was used to extract active substances and identify the levels of antimicrobial active substances. The TLC Spectrophotodensitometer instrument and the diffusion method (discs) were used to test antimicrobial potential. Data analysis was performed using the one-way ANOVA test. All five samples showed flavonoids, alkaloids, tannins, and phenols in qualitative tests. Based on quantitative test results of five samples, the highest compound content obtained in Basa Wangen (6.66 mg/ml of tannins), Basa Gede (3.74 mg/ml of flavonoids), Basa Bawang Jahe (2.49 mg/ml of tannins), Basa Selem (2.87 mg/ml of tannin), and Basa Rajang (6.96 mg/ml of flavonoids). There are differences in the antimicrobial potential of various types of traditional Balinese spices against the growth of Salmonella sp in vitro (sig = 0.037). The traditional Balinese spices have the antimicrobial potential of the intermediate category with a range of inhibition (16-20 mm) based on the NCCLS standard.

Second allogeneic stem cell transplantation in acute leukemia patients: single-centre experience BakÄ±rtaÅŸ, Mehmet; YiÄŸenoÄŸlu, TuÄŸÃ§e Nur; BaÅŸcÄ±, Semih; Uncu Ulu, Bahar; Ã–zcan, NurgÃ¼l; Ä°skender, Dicle; Dal, Mehmet Sinan; KÄ±zÄ±l Ã‡akar, Merih; AltuntaÅŸ, Fevzi
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.208

Acute leukaemia patients who relapse after the first allogeneic stem cell transplantation (Allo-SCT) have a poor prognosis. Participating in clinical trials is the best option for these patients. If patients cannot participate in clinical trials, as the treatment options are limited, the second allo-SCT constitutes the potential curative treatment option. The data of acute leukaemia patients who underwent second allo-SCT because of relapsed/refractory disease after the first allo-SCT at our centre between December 2009 and February 2019 were analyzed retrospectively. Three hundred nineteen acute leukaemia patients were performed allo-SCT at our centre. 20 of these 319 acute leukaemia patients relapsed after first allo-SCT and underwent second allo-SCT. 10 AML patients and 10 ALL patients were included in the study. After second allo-SCT overall survival (OS) was 26.1Â±10.8 weeks, and progression-free survival (PFS) was 19.9Â±8.6 weeks. If the patients cannot participate in clinical trials, second allo-SCT should be considered for patients with late (â‰¥12 months) relapses after the first allo-SCT. If possible, haploidentical donors should be selected for second allo-SCT and patients should be in complete remission before the transplant.

Does platelet/ lymphocyte ratio a predictor of CD34+ peripheral blood stem cell yield in the healthy donors mobilized with GCSF? Gokgoz, Zafer
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29238/teknolabjournal.v9i1.209

This research aimed to determine whether there is a correlation between platelet/lymphocyte ratio the number of collected CD34+ stem cells. This retrospective study included 94 adult related stem cell donors who were healthy and volunteer by screening their files between the years 2016 and 2018. All donors were mobilized using 2.5 mcg/kg lenograstim or filgrastim and underwent peripheral stem cell apheresis. Complete blood counts were tested at baseline before G-CSF administration (preâ€“G-CSF), and before PBSC collection after mobilization with G-CSF administration. The patients were divided into two groups as aged below and over 50 years old. From these comparative data, only BMI value of the group aged below 50 years was statistically significantly lower than the other group, whereas no statistically significant difference was found between the groups in terms of other parameters. The numbers of the collected CD34+ stem cells of both age groups were similar, and no significant difference was found. The values of platelet/lymphocyte ratio, early measurement of CD34+ stem cells and the amount of the collected CD34+ stem cells of both groups at the first and second days of the procedure were found similar. This research show that a high platelet count and consequently, a high platelet/lymphocyte ratio may be correlated with the number of collected CD34+ stem cells but our hypotheses revealed insignificant outcomes.

Modulation of severe acute respiratory syndrome coronavirus (SARS-CoV-2) in receptor, innate immunity and drug antiviral candidate Tarigan, Indra Lasmana; Arum, Kartika
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

The Coronavirus disease-19 (COVID-19) is a contagious acute respiratory infectious disease caused by SARS-CoV-2 as a global pandemic in 2020. This disease most spreads and causes some severe cases, even death in the world. The primary purpose of this review discusses the recent article that was published regarding COVID-19 genomic modulation, the mechanism of innate immunity, and the screening of anti-viral drug candidates, for treating COVID-19 patients. This review used the latest paper regarding COVID-19 with 63 journals with high impact factors such as Nature, The Lancet, Cells, International Journal of Biological Sciences, Mol Biol Methods. Journal of Microbiology, Immunology, and Infection, Nat. Rev. Microbiol, and other international journals indexed by Scopus, Elsevier, and Springer through in vivo and in vitro studies. The genomic of SARS-CoV-2 consist high similarly to coronaviruses family, albeit possessing a different pathway even has higher affinity, due to changing some nitrogen bases are supposed to have a significant effect on its pneumonia. Herein, we report review article an update on the recent literature of the COVID-19 modulation genome, mechanism of innate immunity, and medical literature. Moreover, we report anti-viral drugs that have been developed from synthetic drugs and medicinal compounds from plants. Several studies have been re-analyzed using in vitro, in vivo, and modelling using bioinformatics tools.

Perspective of molecular immune response of SARS-COV-2 infection Rohmah, Martina Kurnia; Rahman Nurdianto, Arif
Jurnal Teknologi Laboratorium Vol 9 No 1 (2020): 2020 (1): Special Edition "COVID-19"
Publisher : POLTEKKES KEMENKES YOGYAKARTA

COVID-19 is a type of Pneumonia caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). When COVID-19 arise in Wuhan China and rapidly spread throughout to the World, we need to learn how pathogenesis and immune responses occur in the bodies in more detail. COVID-19 is the third Severe Respiratory Disease outbreak caused by the Coronavirus in the past two decades after Severe Acute Respiratory Syndrome (SARS) in the 2002 and Middle East Respiratory Syndrome (MERS) in 2012. The Articles from PUBMED and Research Gate were searched for studies on the immune response of COVID-19 infection by SARS-CoV-2. SARS-CoV-2 increases the number of neutrophils, suppresses IFN, increases the activity of Th1/Th17, B cells, CD8+ and CD4+, and causes cytokine storms especially pro-inflammatory cytokines which can increase respiration disorders and multi-organ damage. This review tries to explain about pathogenesis and immune responses of COVID-19 to provide a reference in designing the appropriate immune intervention for treatment and therapeutic such as drug or vaccine based on the recent research progress SARS-CoV-2 and previous studies about SARS CoV and MERS CoV.

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Contact Name
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Contact Email
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Journal Mail Official
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Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA